1989 Fiscal Year Final Research Report Summary
Effect of Multiple-Dose Irradiation on Cultured Mammalian Cells
| Project/Area Number |
63570494
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Yokohama City University |
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Principal Investigator |
MATSUI Kengo Yokohama City University, School of Medicine, Professor, 医学部, 教授 (60117760)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Nobuo Yokohama City University, School of Medicine, Assistant, 医学部, 助手 (20046139)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Multiple-Dose Irradiation / Sublethal Damage / Potentially Lethal Damage / Repair / C3H 10T1 / Cells |
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| Research Abstract |
Using plateau phase C3H 10T1/ cells, we studied the effect of multiple-dose irradiation on the repair capacity of cells after further irradiation. Cells were irradiated with repeated doses of 2.5 Gy delivered two fractions per day 6 to 7 hours interval. The cell survival after exposure to 1 to 9fractions was lower above 5 fractions as compared to that predicted by calculating from single dose survival curve. Repair kinetics experiments showed that cells were less able to repair potentially lethal damage after test dose following multiple dose irradiations. There was, however, no difference in the ability to repair sublethal damage and potentially lethal damage sensitive to anisotonic treatment in preirradiated and untreated cells. Thus, it would appear that an enhancement of the lethal expression of potentially lethal damage only of three types of damage may, at least in part, contribute to the difference between the cell survival curve after multiple fractions and that predicted by calculation.
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