1990 Fiscal Year Final Research Report Summary
Study on Mechanism of Implantation
| Project/Area Number |
63570775
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | 福井医科大学 |
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Principal Investigator |
TOMINAGA Toshiro Fukui Medical School OB-GYN, Professor., 医学部, 教授 (10026908)
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| Project Period (FY) |
1988 – 1990
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| Keywords | Implantation / Rabbit / Blastocyst / Endometrium / In vitro |
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| Research Abstract |
Knowledge of implantation in human beings is urgently needed for the improvement of infertility treatment and fertility control.
In the present study an in vitro model experiment of implantation was carried out using rabbit blastocysts at implantation stage and endometrial tissue reconstituted from purely separated epithelial and stromal cells opposed each other with collagen membrane ling between them.
The results were as follows :
1. Cultures of purely separated epithelial and stromal cells of endometrium :
Enzymatical digestion of endometrial tissues, filtration and short time cultures yielded purely separated epithelial cells and stromal cells.
2. Reconstitution of endometrial tissue in vitro :
Separated epithelial and stromal cells were cultured on opposite sides of collagen membrane, and epithelial cells were cultured on basement membrane extract. These cultures resulted in the formation of endometrial tissues similar to those in vivo.
3. In vitro model experiment of implantation :
Blastocysts at implantation were placed on reconstituted endometrium in vitro. Attachment occurred in 6 hours followed by zona lysis, fine cell membrane linkage formation, and desmosomelike junctional complex and partial cytoplasmic fusion between trophoblast and epithelial cells. This in vitro implantation was accomplished by cooperation between the blastocyst and endometrium.
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