1991 Fiscal Year Final Research Report Summary
On the genesis of trophoblastic deseases using androgenetic and triploid eggs in the mouse
| Project/Area Number |
63570785
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kagawa Medical School |
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Principal Investigator |
HANDO Tamotsu Kagawa Medical School, Department of Perinato-Gynecology, Professsor, 医学部, 教授 (40092720)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Kazuyuki Kagawa Medical School, Department of Perinato-Gynecology, Lecturer, 医学部附属病院, 助手 (80219005)
OHNO Masayuki Kagawa Medical School, Department of Perinato-Gynecology, Assistant Prof., 医学部附属病院, 講師 (40213821)
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| Project Period (FY) |
1988 – 1990
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| Keywords | hydatidiform mole / androgenesis / choriocarcinoma / triploidy |
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| Research Abstract |
Hydatidiform moles can be separated into two entities, complete and partial, on the basis of cytogenetics and histopathology. Complete moles are androgenetic in origin, whereas partial moles are triploid. Some of complete moles have been observed clinically to change to choriocarcinomas, but the mechanism of these changes have not been known. So, we designed a study to investigate the morphologic change of the androgenetic eggs in the mouse. The androgenetic egg was produced bY enucleation of the female pronucleus from the triploid egg with two male pronuclei. About 30 to 40 percent of eggs fertilized in vitro were triploidy, using sperms preincubated in the culture solution supplemented with 20 percent fetal calf serum. A female pronucleus could be aspirated from triploid egg by using a small-boreglass pipette (5 to 10 mum in outer diameter) controlled by a micromanipulator. About 20 percent of enucleated diploid androgenetic eggs developed to the two cell stage in vitro. Twenty androgenetic eggs were transplanted in the oviduct or under the capsule of the kidney and testis, but the development of the eggs was not observed. Forty triploid eggs were transplanted in the oviduct, but the implantation in the uterus was not observed. The androgenetic and triploid eggs have not developed in our study. But when these eggs are observed to change to choriocarcinoma, the chromosomal abnormalities as a cause of carcinogenesis in trophoblastic disease, like other cancer in general as well, might be looked as important etiological factor.
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Research Products
(6 results)
