1990 Fiscal Year Final Research Report Summary
Effect of Drugs for Intravenous Sedation on Human Neutrophil Chemiluminescence
| Project/Area Number |
63570946
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Showa University |
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Principal Investigator |
YOSHIMURA Setsu Showa university, dentistry, associate professor, 歯学部, 助教授 (50112735)
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| Co-Investigator(Kenkyū-buntansha) |
IKUMI Shuji Showa university, dentistry, assistant, 歯学部, 助手 (70211026)
GOTOH Kinuko Showa university, dentistry, assistant, 歯学部, 助手 (70176262)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Chemiluminescence / Neutrophil / Intravenous sedation / Diazepam / Benzodiazepine |
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| Research Abstract |
The chemiluminescence is an index of oxygen free-radical generation, which is an important bactericidal mechanism. The effect of benzodiazepine compound, diazepam on human neutrophil chemiluminescence was evaluated. Chemiluminescence was measured after neutrophil activation of the opsonized zymosan . ht emission was measured using an ATP photometer.
In vitro studies, we found that diazepam produce reductions in chemiluminescence at concentrations of 1 ug/ml to 40ug/ml and the depression was dose dependent. This inhibitory effect of diazepam was reversible and chemiluminescence was recovered after washing out of diazepam.
In clinical practice, we investigated plasma concentrations and the effect of diazepam on human neutrophil chemiluminescence in healthy volunteers received intravenous sedation with diazepam (0.2mg/kg). Mean plasma umiconcentration at three minutes after administration of diazepam was 14.1 <plus-minus>1.9 mu/ml. we found the chemiluminescence was inhibited transiently undergoing intravenous sedation with diazepa
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