1989 Fiscal Year Final Research Report Summary

ACETYLTRANSFERASE (PLATELET-ACTIVATING FACTOR SYNTHESIS) ACTIVITY OF NEUTROPHILS IN INFLAMMATORY DISORDERS: EFFECTS OF CYTOKINES INCLUDING TUMOR NECROSIS FACTOR.

Research Project

Project/Area Number	63571105
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Laboratory medicine
Research Institution	YAMANASHI MEDICAL COLLEGE
Principal Investigator	OZAKI Yukio DEPARTMENT OF CLINICAL AND LABORATORY MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (30134539)
Project Period (FY)	1988 – 1989
Keywords	NEUTROPHILS / PLATELET-ACTIVATING FACTOR / CYTOKINE / TUMOR NECROSIS FACTOR
Research Abstract	Of the five cytokines tested (tumor necrosis factor, interleukin 1, colony-stimulating factor, interferon alpha, beta, and gamma), only tumor necrosis factor (TNF) showed a potent stimulatory effect on the acetyltransferase activity of human neutrophils, which is a key enzyme for the production of platelet-activating factor. This potentiating effect of TNF was time- and dose-dependent and could be effectively blocked by antiserum against TNF. None of Intracellular Ca^<++> elevation, protein kinase C activation, and arachidonic acid metabolites appeared to be involved in the TNF-induced potentiation of the acetyltransferase activity in neutrophils, and the mechanism of TNF potentiation remains to be clarified.