1989 Fiscal Year Final Research Report Summary
Development and automation of enzyme immunoassay of prostanoids
| Project/Area Number |
63870015
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Tokushima University |
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Principal Investigator |
YAMAMOTO Shozo Tokushima University School of Medicine Professor, 医学部, 教授 (50025607)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Yoko Tokushima University School of Medicine Assistant-professor, 医学部, 助手 (60035441)
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| Project Period (FY) |
1988 – 1989
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| Keywords | prostaglandin / thromboxane / bioactive compounds / enzyme immunoassay / monoclonal antibody / radioimmunoassay |
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| Research Abstract |
For the study on the in vivo metabolism of bioactive prostanoids we developed an enzyme-linked immunoassay as a sensitive and specific assay method, and investigated the assay conditions of these prostaglandins in urine and blood. We raised a monoclonal antibody against 11-dehydrothromboxane B_2, which has a longer metabolic half-life than thromboxane B_2 itself and is considered as a reliable parameter of the endogenous thromboxane synthesis. The antibody hardly cross-reacted with thromboxane B_2, and used for radioimmunoassay of 11-dehydrothromboxane. The antibody was also utilized for immunoaffinity extraction and purification of 11-dehydrothromboxane from human urine and blood. The immunoaffinity-purified material was subjected to radioimmunoassay, and the validity of the assay was confirmed by gas chromatography-mass spectrometry.
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