1989 Fiscal Year Final Research Report Summary
Research and Development of New Bone Substitute Using BMP with Atelocollagen
| Project/Area Number |
63870078
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ENOMOTO Shoji TMDU, Oral Surgery, Professor, 歯学部, 教授 (40013940)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Teruo KOKEN Co., I.td., Institute, Head, 日本医用高分子研究所, 所長
NAGURA Hideaki TMDU, Oral Surgery, Assistant Prof., 歯学部, 助教授 (80013960)
ENOMOTO Shoji TMDU, Oral Surgery, Professor (40013940)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Bone Substitute / BMP / Hydroxyapatite / Atelocollagen / Reconstruction of Mandible / Bone-Induction |
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| Research Abstract |
1. Purification of Bone Morphogenetic Protein (BMP): 1) A crude BMP, which was extracted from decalcified bovine bone, was separated with gel filtration, cation exchange chromatography, heparin affinity chromatography, and finally separated with reverse-phase HPLC. SDS-PAGE erectrophoretic examination of the final BMP-fraction showed two smear bands of 16kDa and 30-40kDa under non- reducing conditions, 16kDa and 18kDa tinder reducing conditions. Amino acid sequence analysis revealed the similarity of the protein with a molecular weight of 16kDa to BMP-3 which was reported by J. Wazney et al. in 1988.2) Purification of BMP extracted from human teeth was also performed in the same way as bovine bone. Separation with a series of chromatographies glowed the similarity of elution patterns to that of bovine bone. However, the final purification is under investigation.
2. Examination of BMP-composite (AC-BMP, HA-AC-BMP) by implantation into bone- defects: 1)Aterocoliagen-BMP composite (AC-BMP)
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and liydroxyapatite-atelocollagen -BMP composite (HA-AC-BMP) (both were lyophilized and compressed), HA/AC/BMP mixture (gel) were implanted intoskull defects of rats. Both AC-GMP composite and HA-AC-BMP composite consistently induced the most remarkable new-bone formation from early stage. The repair of defects which had been implanted with AC-BMP composite and HA-AC-BMP composite were the most rapid and complete. 2) HA-AC-BMP composite was also implanted into mandible of monkey. Three months after implantation, the defect was completely regenerated by newly formed bone in the implantation of RA-AC-BMP composite, while small amount of new bone was formed only at the rim of the host bed in the implantation of HA-AC composite as control.
These results indicate that AC-BMP composite and HA-AC-BMP composite have excellent bone-inducing and bone-regenerating potency. Results from the Primates suggest the possibility of these BMP-composite (AC-BMP, HA-AC-BMP) inclinical use as bone-inductive biomaterials in place of fresh autogenous bone. Less
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Research Products
(3 results)
