Drug delivery system for the clinical application of cytokines

1989 Fiscal Year Final Research Report Summary

• Project/Area Number: 63870095
• Research Category: Grant-in-Aid for Developmental Scientific Research
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: University of Tokyo
• Principal Investigator: OSAWA Toshiaki Univ. of Tokyo, Fac. of Pharm. Sci., Professor, 薬学部, 教授 (40012603)
• Co-Investigator(Kenkyū-buntansha): HIGUCHI Masahiro Denki Kagaku Kogyo Co., Research fellow, 中央研究所, 研究員 ; IMAI Yasuyuki Univ. of Tokyo, Fac. of Pharm. Sci., Research associate, 薬学部, 助手 (80160034)
• Project Period (FY): 1988 – 1989
• Keywords: Immunoliposomes / Lymphotoxin / Interferon-gamma / Nude mouse / Human melanoma A375

Research Abstract

Recombinant human LT was encapsulated into immunoliposomes on which monoclonal anti-A375 antibody was conjugated. These immunoliposomes showed stronger cytotoidcity toward A375 cells in in vitro experiments and also in vivo experiments. When mouse IFN-Y was encapsulated in anti-A375-conjugated immunoliposomes together with human LT and those immunoliposomes were administered into nude mice twice a week starting from 2 days after implantation of A375 cells, significantly stronger cytotoxicity was observed compared with the experiments using immunoliposomes in which only human LT was encapsulated. These results clearly indicated that at least a part of cytotoxicity of LT-immunoliposomes was exerted through the immune system of host animals.