| Project/Area Number |
63880021
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | Kyoto University |
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Principal Investigator |
SODA Kenji Kyoto University, Institute for Chemical Research, Professor, 化学研究所, 教授 (30027023)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Kousuke Unitica Co. Ltd., Director, 中央研究所, 部長
YOSHIMURA Tohru Kyoto Univ., Inst. for Chem. Res., Assistant Prof., 化学研究所, 助手 (70182821)
TANIZAWA Katsuyuki Osaka Univ., Inst. Sci. Indus. Res., Associate Prof., 産業科学研究所, 助教授 (20133134)
ESAKI Nobuyoshi Kyoto Univ., Inst. for Chem. Res., Associate Prof., 化学研究所, 助教授 (50135597)
TANAKA Hidehiko Okayama Univ., Fac. of Agric., Prof., 農学部, 教授 (90065912)
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| Project Period (FY) |
1988 – 1990
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| Keywords | Amino acid / Vitamin / Microbial enzyme / Isotope-labeling |
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| Research Abstract |
New methods on isotope-labeling of amino acid and vitamin were established with microbial enzymes, which have high stereo-specificity of reaction. First, or ^<35>S- labeling ofS-alkylcysteine was established by beta -replacement reaction of S-alkylcysleine alpha, beta -lyase, which was newly purified from a thermophile (Bacillus sp. 41A). Second, deuterium or tritium-labeling of D-amino acid was developed Stereoselectively with D-amino acid aminotransferase. Stereoselectively-deulerated NADH and NADPH were also prepared by coupling of glutamate racemase and glutamate dehydrogenase. Further, double isotope-labeling of Lysine was carried out by L-lysine epsilon -dehydrogenase and amino acid racemase. Finally. Substitution of S- (beta -aminoethyl) - cysteine for active-site lysine of thermostable aspartate aminotransferase was established by gene technology and chemical modification. Thus, many effective isotope-labeling of amino acid, vitamin and protein were developed.
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