2021 Fiscal Year Annual Research Report
Site-Selective Protein Chemical Modification of Exposed Tyrosine Residues Using Tyrosine Click Reaction
Publicly Offered Research
| Project Area | Non-equilibrium-state molecular movies and their applications |
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| Project/Area Number |
20H05438
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Maity Basudev 東京工業大学, 生命理工学院, 特任助教 (60815421)
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | CO release reaction / Initial intermediate / TR-SFX method |
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| Outline of Annual Research Achievements |
In this research, we demonstrated a visualization of photolysis reaction by a synthetic metal complex using TR-SFX method. We fixed a photoactive CO releasing Mn(CO)3 reaction center into porous Lysozyme microcrystals. X-ray crystal structure revealed an octahedral Mn reaction center with 3CO and two water molecules. Three CO ligands oriented in a facial manner. By controlling the time and light dose, we succeeded in determining the CO release process and structure of a bicarbonyl intermediate species in real-time. In addition, we performed quantum chemical calculations which was in good agreement with the experimental results. The demonstration has a great scope to explore the mechanism of difficult organic, inorganic, or synthetic metalloprotein reactions.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Research Products
(2 results)
