Zonation of auxin responsiveness to control the periodicity of stem cell amplification

2023 Fiscal Year Annual Research Report

• Project Area: Intrinsic periodicity of cellular systems and its modulation as the driving force behind plant development
• Project/Area Number: 22H04733
• Research Institution: Tokyo University of Science
• Principal Investigator: 西浜 竜一 東京理科大学, 創域理工学部生命生物科学科, 教授 (70283455)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: 幹細胞 / 二叉分枝 / オーキシン / 細胞感コミュニケーション / ゼニゴケ / 転写因子

Outline of Annual Research Achievements

植物は周期的に幹細胞群を新たにつくり、枝分かれを行う。ゼニゴケは、葉状体の先端に存在する頂端幹細胞を周期的に増幅して二叉分枝を行うが、その際の幹細胞の動態はよくわかっていなかった。植物ホルモン・オーキシンへの応答性が、幹細胞ゾーンでは低く、その周辺では高いことが示唆されたため、オーキシン応答マーカーを確立して、その動態を追う予定であったが期間内には確立できなかった。しかし、幹細胞ゾーンで特異的に発現する転写因子（S）と、オーキシン応答促進に関わる転写因子（A）の発現レポーターを用いて、ライブイメージング観察を行ったところ、分枝進行に伴ってAの発現が低い領域が拡大し、Sの発現はその領域中で揺らぎながら最終的に両端に固定される様子が観察された。よって、新しい幹細胞は元の幹細胞から直接生じるのではなく、低オーキシン応答性領域が拡大し、その両端に新たに形成されることが示唆された。
AとSの標的候補として前年度に同定した、分泌性ペプチドとその推定受容体の遺伝子の発現レポーター株を観察したところ、AとSの発現部位とよく合致する発現パターンが観察された。ペプチド遺伝子の誘導過剰発現株で発現誘導を行ったところ、幹細胞ゾーンが乱れるとともに、メリステム内で分化に向かう細胞がより早く分化すると考えられる表現型が見られた。よって、このペプチドを介した細胞間コミュニケーションが、オーキシン応答性制御と協調して幹細胞性を制御することが示唆され、それが幹細胞動態、およびオーキシン応答性の区画化に及ぼす影響をさらに解析することで、二叉分枝のより詳細なメカニズムの解明が期待される。

Research Progress Status

令和5年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和5年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] National Taiwan University(その他の国・地域)
  • Country Name: その他の国・地域
  • Counterpart Institution: National Taiwan University
• [Int'l Joint Research] University of Edinburgh(英国)
  • Country Name: UNITED KINGDOM
  • Counterpart Institution: University of Edinburgh
• [Int'l Joint Research] Wageningen University(オランダ)
  • Country Name: NETHERLANDS
  • Counterpart Institution: Wageningen University
• [Int'l Joint Research] Monash University(オーストラリア)
  • Country Name: AUSTRALIA
  • Counterpart Institution: Monash University
• [Int'l Joint Research] Max Planck Institute(ドイツ)
  • Country Name: GERMANY
  • Counterpart Institution: Max Planck Institute
• [Journal Article] A non-canonical BZR/BES transcription factor regulates the development of haploid reproductive organs in Marchantia polymorpha (2024)
  • Author(s): Furuya Tomoyuki、Saegusa Natsumi、Yamaoka Shohei、Tomoita Yuki、Minamino Naoki、Niwa Masaki、Inoue Keisuke、Yamamoto Chiaki、Motomura Kazuki、Shimadzu Shunji、Nishihama Ryuichi、Ishizaki Kimitsune、Ueda Takashi、Fukaki Hidehiro、Kohchi Takayuki、Fukuda Hiroo、Kasahara Masahiro、Araki Takashi、Kondo Yuki
  • Journal Title: Nature Plants Volume: n/a Pages: n/a
  • DOI: 10.1038/s41477-024-01669-0
  • Peer Reviewed
• [Journal Article] Dual Regulation of Cytochrome P450 Gene Expression by Two Distinct Small RNAs, a Novel tasiRNA and miRNA, in <i>Marchantia polymorpha</i> (2024)
  • Author(s): Hung Yu-Ling、Hong Syuan-Fei、Wei Wei-Lun、Cheng Shiuan、Yu Jia-Zhen、Tjita Veny、Yong Qian-Yuan、Nishihama Ryuichi、Kohchi Takayuki、Bowman John L、Chien Yuan-Chi、Chiu Yen-Hsin、Yang Ho-Chun、Lu Mei-Yeh Jade、Pan Zhao-Jun、Wang Chun-Neng、Lin Shih-Shun
  • Journal Title: Plant And Cell Physiology Volume: n/a Pages: pcae029
  • DOI: 10.1093/pcp/pcae029
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Convergent evolution of water-conducting cells in Marchantia recruited the ZHOUPI gene promoting cell wall reinforcement and programmed cell death (2024)
  • Author(s): Lu YT, Loue-Manifel J, Bollier N, Gadient P, De Winter F, Carella P, Hoguin A, Grey-Switzman S, Marnas H, Simon F, Copin A, Fischer S, de Leau E, Schornack S, Nishihama R, Kohchi T, Depege Fargeix N, Ingram G, Nowack MK, Goodrich J.
  • Journal Title: Current Biology Volume: 34 Pages: 793～807.e7
  • DOI: 10.1016/j.cub.2024.01.014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane H+-ATPase (2024)
  • Author(s): Fuji Saashia、Yamauchi Shota、Sugiyama Naoyuki、Kohchi Takayuki、Nishihama Ryuichi、Shimazaki Ken-ichiro、Takemiya Atsushi
  • Journal Title: Nature Communications Volume: 15 Pages: 1195
  • DOI: 10.1038/s41467-024-45236-9
  • Peer Reviewed / Open Access
• [Journal Article] Conserved CKI1-mediated signaling is required for female germline specification in Marchantia polymorpha (2024)
  • Author(s): Bao Haonan、Sun Rui、Iwano Megumi、Yoshitake Yoshihiro、Aki Shiori S.、Umeda Masaaki、Nishihama Ryuichi、Yamaoka Shohei、Kohchi Takayuki
  • Journal Title: Current Biology Volume: 34 Pages: 1324～1332.e6
  • DOI: 10.1016/j.cub.2024.01.013
  • Peer Reviewed
• [Journal Article] The phosphorylated pathway of serine biosynthesis affects sperm, embryo, and sporophyte development, and metabolism in Marchantia polymorpha (2024)
  • Author(s): Wang Mengyao、Tabeta Hiromitsu、Ohtaka Kinuka、Kuwahara Ayuko、Nishihama Ryuichi、Ishikawa Toshiki、Toyooka Kiminori、Sato Mayuko、Wakazaki Mayumi、Akashi Hiromichi、Tsugawa Hiroshi、Shoji Tsubasa、Okazaki Yozo、Yoshida Keisuke、Sato Ryoichi、Ferjani Ali、Kohchi Takayuki、Hirai Masami Yokota
  • Journal Title: Communications Biology Volume: 7 Pages: 102
  • DOI: 10.1038/s42003-023-05746-6
  • Peer Reviewed / Open Access
• [Journal Article] Transcription of the Antisense Long Non-Coding RNA, <i>SUPPRESSOR OF FEMINIZATION</i>, Represses Expression of the Female-Promoting Gene <i>FEMALE GAMETOPHYTE MYB</i> in the Liverwort <i>Marchantia polymorpha</i> (2024)
  • Author(s): Kajiwara Tomoaki、Miyazaki Motoki、Yamaoka Shohei、Yoshitake Yoshihiro、Yasui Yukiko、Nishihama Ryuichi、Kohchi Takayuki
  • Journal Title: Plant And Cell Physiology Volume: 65 Pages: 338～349
  • DOI: 10.1093/pcp/pcad170
  • Peer Reviewed / Open Access
• [Journal Article] RAF-like protein kinases mediate a deeply conserved, rapid auxin response (2024)
  • Author(s): Kuhn Andre、Roosjen Mark、Mutte Sumanth、Dubey Shiv Mani、Carrillo Carrasco Vanessa Polet、Boeren Sjef、Monzer Aline、Koehorst Jasper、Kohchi Takayuki、Nishihama Ryuichi、Fendrych Maty??、Sprakel Joris、Friml Ji??、Weijers Dolf
  • Journal Title: Cell Volume: 187 Pages: 130～148.e17
  • DOI: 10.1016/j.cell.2023.11.021
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hormonal and genetic control of pluripotency in bryophyte model systems (2024)
  • Author(s): Flores-Sandoval Eduardo、Nishihama Ryuichi、Bowman John L.
  • Journal Title: Current Opinion in Plant Biology Volume: 77 Pages: 102486～102486
  • DOI: 10.1016/j.pbi.2023.102486
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] LysM-mediated signaling in Marchantia polymorpha highlights the conservation of pattern-triggered immunity in land plants (2023)
  • Author(s): Yotsui, I., Matsui, H., Miyauchi, S., Iwakawa, H., Melkonian, K., Schluter, T., Michavila, S., Kanazawa, T., Nomura, Y., Stolze, S.C., Jeon, H.W., Yan, Y., Harzen, A., Sugano, S.S., Shirakawa, M., Nishihama, R., Ichihashi, Y., Ibanez, S.G., Shirasu, K., Ueda, T., Kohchi, T., Nakagami, H.
  • Journal Title: Current Biology Volume: 33 Pages: 3732～3746.e8
  • DOI: 10.1016/j.cub.2023.07.068
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Harnessing Deep Learning to Analyze Cryptic Morphological Variability of?<i>Marchantia polymorpha</i> (2023)
  • Author(s): Tomizawa Yoko、Minamino Naoki、Shimokawa Eita、Kawamura Shogo、Komatsu Aino、Hiwatashi Takuma、Nishihama Ryuichi、Ueda Takashi、Kohchi Takayuki、Kondo Yohei
  • Journal Title: Plant And Cell Physiology Volume: 64 Pages: 1343～1355
  • DOI: 10.1093/pcp/pcad117
  • Peer Reviewed / Open Access
• [Journal Article] A bHLH heterodimer regulates germ cell differentiation in land plant gametophytes (2023)
  • Author(s): Saito Misaki、Momiki Ryosuke、Ebine Kazuo、Yoshitake Yoshihiro、Nishihama Ryuichi、Miyakawa Takuya、Nakano Takeshi、Mitsuda Nobutaka、Araki Takashi、Kohchi Takayuki、Yamaoka Shohei
  • Journal Title: Current Biology Volume: 33 Pages: 4980～4987.e6
  • DOI: 10.1016/j.cub.2023.09.020
  • Peer Reviewed
• [Journal Article] Biosynthesis of gibberellin-related compounds modulates far-red light responses in the liverwort <i>Marchantia polymorpha</i> (2023)
  • Author(s): Sun Rui、Okabe Maiko、Miyazaki Sho、Ishida Toshiaki、Mashiguchi Kiyoshi、Inoue Keisuke、Yoshitake Yoshihiro、Yamaoka Shohei、Nishihama Ryuichi、Kawaide Hiroshi、Nakajima Masatoshi、Yamaguchi Shinjiro、Kohchi Takayuki
  • Journal Title: The Plant Cell Volume: 35 Pages: 4111～4132
  • DOI: 10.1093/plcell/koad216
  • Peer Reviewed
• [Presentation] 幹細胞因子MpLAXRの発現パターンから見るゼニゴケ二叉分枝の解析 (2024)
  • Author(s): 今井雄星, 灰庭瑛実, 岩木彩, 石田咲子, 河内孝之, 西浜竜一
  • Organizer: 日本植物生理学会第65回年会
• [Presentation] Analysis of regeneration control by the MAP kinase MpMPK1 in Marchantia polymorpha (2024)
  • Author(s): Rika Yanuki, Shota Yamauchi, Ryuichi Nishihama
  • Organizer: 日本植物生理学会第65回年会
• [Presentation] Regulatory pathway for decapitation-triggered cellular reprogramming in the liverwort Marchantia polymorpha (2023)
  • Author(s): Ryuichi Nishihama
  • Organizer: International Plant Regeneration Workshop “Molecular Insights into Plant Regeneration: From Cells to Tissues and Beyond”
  • Int'l Joint Research / Invited
• [Presentation] Involvement of MAP kinase signaling in regeneration of Marchantia polymorpha (2023)
  • Author(s): Rika Yanuki, Shota Yamauchi, Ryuichi Nishihama
  • Organizer: International Plant Regeneration Workshop “Molecular Insights into Plant Regeneration: From Cells to Tissues and Beyond”
  • Int'l Joint Research
• [Presentation] Analysis of the functions of the bHLH transcription factor MpLRL in apical growth and regeneration in Marchantia polymorpha (2023)
  • Author(s): Haruka Mine, Aya Iwaki, Emi Hainiwa, Shohei Yamaoka, Takayuki Kohchi, Ryuichi Nishihama
  • Organizer: International Plant Regeneration Workshop “Molecular Insights into Plant Regeneration: From Cells to Tissues and Beyond”
• [Presentation] Involvement of MAP kinase signaling in regeneration of Marchantia polymorpha (2023)
  • Author(s): Rika Yanuki, Shota Yamauchi, Ryuichi Nishihama
  • Organizer: Taiwan Japan Plant Biology 2023
  • Int'l Joint Research
• [Presentation] Establishment and maintenance of stemness by limiting auxin responsiveness in Marchantia polymorpha (2023)
  • Author(s): Yusei Imai, Emi Hainiwa, Sakiko Ishida, Aya Iwaki, Takeshi Kinami, Kohchi Takayuki, Ryuichi Nishihama
  • Organizer: Taiwan Japan Plant Biology 2023
  • Int'l Joint Research / Invited
• [Presentation] Phosphorylation of two Thr residues in the C-terminal auto-inhibitory domain of plasma membrane H+-ATPase is crucial for light-induced stomatal opening (2023)
  • Author(s): Fuji, S., Yamauchi, S., Sugiyama, N., Kohchi, T., Nishihama, R., Shimazaki, K. Takemiya, A.
  • Organizer: 33rd International Conference on Arabidopsis Research
  • Int'l Joint Research
• [Presentation] 三次元頂端成長の確立とオーキシン~ゼニゴケの信号伝達経路の役割から探る~ (2023)
  • Author(s): 西浜竜一、鈴木秀政、河内孝之
  • Organizer: 日本植物学会第87回大会シンポジウム「植物の陸上進出の鍵となった細胞機能改変」
  • Invited
• [Remarks] 東京理科大学創域理工学部生命生物科学科西浜研究室
  • URL: https://www.rs.tus.ac.jp/nishihama/