2023 Fiscal Year Annual Research Report

Direct visualization of the excited state structural dynamics of a synthetic Cu(I)-phenanthroline complex by TR-SFX method

Publicly Offered Research

Project Area	Non-equilibrium-state molecular movies and their applications
Project/Area Number	22H04744
Research Institution	Tokyo Institute of Technology
Principal Investigator	Maity Basudev 東京工業大学, 生命理工学院, 特任助教 (60815421)
Project Period (FY)	2022-04-01 – 2024-03-31
Keywords	TR-SFX / Mn-carbonyl complex / Lysozyme / Serial crystallography / Reaction intermediate
Outline of Annual Research Achievements	We immobilized a light-sensitive Mn-carbonyl complex in the porous lysozyme crystal and characterized by ATR-IR and absorbance spectroscopy. We determined the structure under darkness and compared with metal-free protein. We applied TR-SFX method to determine the changes at the metal reaction center after light irradiation. This gave interesting features such as releasing of CO ligand sequentially and the intermediate in which a water molecule was coordinated. The release of CO ligand was estimated by considering the appearance of negative density features after light irradiation which was were increased with increasing delay time. High laser intensity was found to trigger the CO release reaction. The results were validated by the QM MM calculations.
Research Progress Status	令和5年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	令和5年度が最終年度であるため、記入しない。

(3 results)