The Germline: Its Developmental Cycle and Epigenome Network

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 19061001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University (2010-2012); National Institute of Genetics (2007-2009)
• Principal Investigator: SASAKI Hiroyuki 九州大学, 生体防御医学研究所, 教授 (30183825)
• Co-Investigator(Kenkyū-buntansha): MATSUI Yasuhisa 東北大学, 加齢医学研究所, 教授 (40241575) ; 阿部 訓也 独立行政法人理化学研究所, 動物変異動態解析技術開発チーム, チームリーダー (40240915) ; 小倉 淳郎 独立行政法人理化学研究所, 遺伝工学基盤技術室, 室長 (20194524) ; 中辻 憲夫 京都大学, 物資-細胞統合システム拠点, 教授 (80237312) ; 仲野 徹 大阪大学, 生命機能研究科, 教授 (00172370)
• Co-Investigator(Renkei-kenkyūsha): ABE Kuniya 独立行政法人理化学研究所, 動物変異動態解析技術開発チーム, チームリーダー (40240915) ; OGURA Atsuo 独立行政法人理化学研究所, 遺伝工学基盤技術室, 室長 (20194524) ; NAKATSUJI Norio 京都大学, 物質-細胞統合システム拠点, 教授 (80237312) ; NAKANO Toru 大阪大学, 生命機能研究科, 教授 (00172370)
• Project Period (FY): 2007 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥64,400,000 (Direct Cost: ¥64,400,000); Fiscal Year 2012: ¥9,200,000 (Direct Cost: ¥9,200,000); Fiscal Year 2011: ¥9,200,000 (Direct Cost: ¥9,200,000); Fiscal Year 2010: ¥18,400,000 (Direct Cost: ¥18,400,000); Fiscal Year 2009: ¥9,200,000 (Direct Cost: ¥9,200,000); Fiscal Year 2008: ¥9,200,000 (Direct Cost: ¥9,200,000); Fiscal Year 2007: ¥9,200,000 (Direct Cost: ¥9,200,000)
• Keywords: 発生・分化 / 発現制御 / 生殖細胞 / エピゲノム / リプログラミング

Research Abstract

As the management team of the Grants-in-Aid for Scientific Research on Priority Areas titled “The Germline: Its Developmental Cycle and Epigenome Network”, we organized various scientific and social activities and promoted three major research projects: 1) cell fate determination for germline development; 2) epigenomic regulation in gametogenesis and meiosis; 3) epigenomic reprogramming in fertilization and early development. Our major scientific outcomes are: in vitro induction of primordial germ cells from pluripotent stem cells; discovery and correction of aberrant X-chromosome inactivation in cloned mice; dissection of the epigenomic regulatory networks in germ cells. To share the latest research results with the community, we made a website in Japanese and English and hosted four domestic and one international symposium. We also supported young scientists’ forum.

Research Products

• [Journal Article] Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice (2012)
  • Author(s): Hayashi, K., Ogushi, S., Kurimoto, K., Shimamoto, S., Ohta, H., Saitou, M
  • Journal Title: Science Volume: 338 Issue: 6109 Pages: 971-975
  • DOI: 10.1126/science.1226889
  • Peer Reviewed
• [Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5MeC to 5HmC in early embryos (2012)
  • Author(s): Nakamura, T., Liu, Y.U., Nakashima, H., Umehara, H., Inoue, K., Matoba, S., Tachibana, M., Ogura, A., Shinkai, Y., Nakano, T
  • Journal Title: Nature Volume: 486 Issue: 7403 Pages: 415-419
  • DOI: 10.1038/nature11093
  • Peer Reviewed
• [Journal Article] Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells (2011)
  • Author(s): 林克彦, 栗本一基, 大田浩, 荒牧伸弥, 斎藤通紀
  • Journal Title: Cell Volume: 146 Issue: 4 Pages: 519-532
  • DOI: 10.1016/j.cell.2011.06.052
  • Peer Reviewed
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus (2011)
  • Author(s): Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D., Sasaki, H
  • Journal Title: Science Volume: 332 Issue: 6031 Pages: 848-52
  • DOI: 10.1126/science.1203919
  • Peer Reviewed
• [Journal Article] The way of the germline: its developmental cycle and epigenome network (2011)
  • Author(s): Chuva de Sousa Lopes SM, Roelen BA
  • Journal Title: Differentiation Volume: 81 Issue: 4 Pages: 217-221
  • DOI: 10.1016/j.diff.2011.02.003
• [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer (2010)
  • Author(s): Inoue, K., Kohda T., Sugimoto, M., Sado T., Ogonuki, N., Matoba, S., Shiura, H., Ikeda, R., Mochida, K., Fujii, T., Sawai, K., Otte, A.P, Tian, X.C., Yang, X., Ishino, F., Abe, K., Ogura, A
  • Journal Title: Science Volume: 330 Issue: 6003 Pages: 496-499
  • DOI: 10.1126/science.1194174
  • Peer Reviewed
• [Journal Article] Proviral silencing in embryonic stem cells requires the histone methyltrans-ferase ESET (2010)
  • Author(s): Matsui, T., Leung, D., Miyashita, H., Maksakova, I.A., Miyachi, H., Kimura, H., Tachibana, M., Lorincz, M.C., Shinkai, Y
  • Journal Title: Nature Volume: 464 Issue: 7290 Pages: 927-931
  • DOI: 10.1038/nature08858
  • Peer Reviewed
• [Journal Article] Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET. (2010)
  • Author(s): Matsui, T., Leung, D., Miyashita, H., Maksakova, I.A., Miyachi, H., Kimura, H., Tachibana, M., Lorincz, M.C., Shinkai, Y.
  • Journal Title: Nature 464 Pages: 927-931
• [Journal Article] Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation. (2010)
  • Author(s): Kanatsu-Shinohara, M., Takashima, S., Shinohara, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA 107 Pages: 6210-6215
• [Journal Article] A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila (2009)
  • Author(s): Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., Kotani, H., Asai, K., Siomi, H., Siomi, MC
  • Journal Title: Nature Volume: 461 Issue: 7268 Pages: 1296-1299
  • DOI: 10.1038/nature08501
• [Journal Article] A signaling principle for the specification of the germ cell lineage in mice. (2009)
  • Author(s): Ohinata, Y., Ohta, H., Shigeta, M., Yamanaka, K., Wakayama, T., Saitou, M.
  • Journal Title: Cell 137 Pages: 398-400
• [Journal Article] Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-Cyclin D2 activation. (2009)
  • Author(s): Lee, J., Kanatsu-Shinohara, M., Morimoto, H., Kazuki, Y., Takashima, S., Oshimura, M., Toyokuni, S., Shinohara, T
  • Journal Title: Cell Stem Cell 5 Pages: 76-86
• [Journal Article] A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. (2009)
  • Author(s): Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., Kotani, H., Asai, K., Siomi, H., Siomi. M.C.
  • Journal Title: Nature 461 Pages: 1296-1299
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. (2009)
  • Author(s): Shoji, M., Tanaka, T., Hosokawa, M., Reuter, M., Stark, A., Kato, Y., Kondoh, G., Okawa, K., Chujo, T., Suzuki, T., Hata, K., Martin, S.L., Noce, T., Kuramochi-Miyagawa, S., Nakano, T., Sasaki, H., Pillai, R.S., Nakatsuji, N., Chuma, S
  • Journal Title: Dev. Cell 17 Pages: 1-13
• [Journal Article] Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes (2008)
  • Author(s): Watanabe, T., Totoki, Y., Toyoda, A., Kaneda, M., Kuramochi-Miyagawa, S., Obata, Y., Chiba, H., Kohara, Y., Kono, T., Nakano, T., Surani, M.A., Sakaki, Y., Sasaki, H
  • Journal Title: Nature Volume: 453 Issue: 7194 Pages: 539-543
  • DOI: 10.1038/nature06908
• [Journal Article] DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. (2008)
  • Author(s): Kuramochi-Miyagawa, S., Watanabe, T., Gotoh, K., Totoki, Y., Toyoda, A., Ikawa, M., Asada, N., Kojima, K., Yamaguchi, Y., Ijiri, T.W., Hata, K., Li, E., Matsuda, Y., Kimura, T., Okabe, M., Sakaki, Y., Sasaki, H., Nakano, T.
  • Journal Title: Genes Dev. 22 Pages: 908-917
• [Journal Article] Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes. (2008)
  • Author(s): Watanabe, T., Totoki, Y., Toyoda, A., Kaneda, M., Kuramochi-Miyagawa, S., Obata, Y., Chiba, H., Kohara, Y., Kono, T., Nakano, T., Surani, M.A., Sasaki, Y., Sasaki, H.
  • Journal Title: Nature 453 Pages: 539-543
• [Journal Article] Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. (2008)
  • Author(s): Hirasawa, R., Chiba, H., Kaneda, M., Tajima, S., Li, E., Jaenisch, R., Sasaki, H.
  • Journal Title: Genes Dev. 22 Pages: 1607-1616
• [Journal Article] Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice. (2008)
  • Author(s): Kurimoto, K., Yabuta, Y., Ohinata, Y., Shigeta, M., Yamanaka, K., Saitou M.
  • Journal Title: Genes Dev. 22 Pages: 1617-1635
• [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice. (2008)
  • Author(s): Yamaji, M., Seki, Y., Kurimoto, K., Yabuta, Y., Yuasa, M., Shigeta, M, Yamanaka, K., Ohinata, Y., Saitou, M.
  • Journal Title: Nat. Genet. 40 Pages: 1016-1022
• [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends onβ1-integrin. (2008)
  • Author(s): Kanatsu-Shinohara, M., Takehashi, M., Takashima, S., Lee, J., Morimoto, H., Chuma, S., Raducanu, A., Nakatsuji, N., Fassler R., Shinohara, T.
  • Journal Title: Cell Stem Cell 3 Pages: 533-542
• [Book] A global single-cell cDNA amplification method for quantitative microarray analysis. In "PCR Protocols 3rd edition" Ed. by Danny Park (Humana Press, USA) (2011)
  • Author(s): Kurimoto, K. and Saitou, M
  • Publisher: Methods in Molecular Biology
• [Book] 「卵子学」(森 崇英・総編集) (2011)
  • Author(s): 青木不学、小倉淳郎、河村和弘、斎藤通紀、河野友宏、佐々木裕之、田中智、松居靖久
  • Publisher: 京都大学学術出版会
• [Book] Germline modification using mouse spermatogonial stem cells (2010)
  • Author(s): Kanatsu-Shinohara, M. and Shinohara, T
  • Publisher: Methods Enzymol
• [Book] Somatic cell nuclear transfer in the mouse. In "Microinjection: Methods and Applications Ed. by David J. Carroll (Humana Press, USA) (2009)
  • Author(s): Kishigami, S. and Wakayama, T
  • Publisher: Methods in Molecular Biology
• [Book] 「クローンマンモスへの道-クローン技術最前線の技術における発生・再生医療技術を探る 」(単著) (2009)
  • Author(s): 若山照彦
  • Publisher: アドスリー社
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html#
• [Remarks] 領域ホームページ
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html
• [Remarks] 領域ホームページ
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html
• [Remarks] 領域ホームページ
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html
• [Remarks] 領域ホームページ
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html
• [Remarks] 領域ホームページ
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html