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The Germline: Its Developmental Cycle and Epigenome Network

Administrative Group

Project AreaThe germline: its developmental cycle and epigenome network
Project/Area Number 19061001
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyushu University (2010-2012)
National Institute of Genetics (2007-2009)

Principal Investigator

SASAKI Hiroyuki  九州大学, 生体防御医学研究所, 教授 (30183825)

Co-Investigator(Kenkyū-buntansha) MATSUI Yasuhisa  東北大学, 加齢医学研究所, 教授 (40241575)
阿部 訓也  独立行政法人理化学研究所, 動物変異動態解析技術開発チーム, チームリーダー (40240915)
小倉 淳郎  独立行政法人理化学研究所, 遺伝工学基盤技術室, 室長 (20194524)
中辻 憲夫  京都大学, 物資-細胞統合システム拠点, 教授 (80237312)
仲野 徹  大阪大学, 生命機能研究科, 教授 (00172370)
Co-Investigator(Renkei-kenkyūsha) ABE Kuniya  独立行政法人理化学研究所, 動物変異動態解析技術開発チーム, チームリーダー (40240915)
OGURA Atsuo  独立行政法人理化学研究所, 遺伝工学基盤技術室, 室長 (20194524)
NAKATSUJI Norio  京都大学, 物質-細胞統合システム拠点, 教授 (80237312)
NAKANO Toru  大阪大学, 生命機能研究科, 教授 (00172370)
Project Period (FY) 2007 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥64,400,000 (Direct Cost: ¥64,400,000)
Fiscal Year 2012: ¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 2011: ¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 2010: ¥18,400,000 (Direct Cost: ¥18,400,000)
Fiscal Year 2009: ¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 2008: ¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 2007: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywords発生・分化 / 発現制御 / 生殖細胞 / エピゲノム / リプログラミング
Research Abstract

As the management team of the Grants-in-Aid for Scientific Research on Priority Areas titled “The Germline: Its Developmental Cycle and Epigenome Network”, we organized various scientific and social activities and promoted three major research projects: 1) cell fate determination for germline development; 2) epigenomic regulation in gametogenesis and meiosis; 3) epigenomic reprogramming in fertilization and early development. Our major scientific outcomes are: in vitro induction of primordial germ cells from pluripotent stem cells; discovery and correction of aberrant X-chromosome inactivation in cloned mice; dissection of the epigenomic regulatory networks in germ cells. To share the latest research results with the community, we made a website in Japanese and English and hosted four domestic and one international symposium. We also supported young scientists’ forum.

Report

(8 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report

Research Products

(34 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (21 results) (of which Peer Reviewed: 6 results) Book (5 results) Remarks (8 results)

  • [Journal Article] Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice2012

    • Author(s)
      Hayashi, K., Ogushi, S., Kurimoto, K., Shimamoto, S., Ohta, H., Saitou, M
    • Journal Title

      Science

      Volume: 338 Issue: 6109 Pages: 971-975

    • DOI

      10.1126/science.1226889

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5MeC to 5HmC in early embryos2012

    • Author(s)
      Nakamura, T., Liu, Y.U., Nakashima, H., Umehara, H., Inoue, K., Matoba, S., Tachibana, M., Ogura, A., Shinkai, Y., Nakano, T
    • Journal Title

      Nature

      Volume: 486 Issue: 7403 Pages: 415-419

    • DOI

      10.1038/nature11093

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells2011

    • Author(s)
      林克彦, 栗本一基, 大田浩, 荒牧伸弥, 斎藤通紀
    • Journal Title

      Cell

      Volume: 146 Issue: 4 Pages: 519-532

    • DOI

      10.1016/j.cell.2011.06.052

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus2011

    • Author(s)
      Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D., Sasaki, H
    • Journal Title

      Science

      Volume: 332 Issue: 6031 Pages: 848-52

    • DOI

      10.1126/science.1203919

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The way of the germline: its developmental cycle and epigenome network2011

    • Author(s)
      Chuva de Sousa Lopes SM, Roelen BA
    • Journal Title

      Differentiation

      Volume: 81 Issue: 4 Pages: 217-221

    • DOI

      10.1016/j.diff.2011.02.003

    • Related Report
      2012 Final Research Report
  • [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer2010

    • Author(s)
      Inoue, K., Kohda T., Sugimoto, M., Sado T., Ogonuki, N., Matoba, S., Shiura, H., Ikeda, R., Mochida, K., Fujii, T., Sawai, K., Otte, A.P, Tian, X.C., Yang, X., Ishino, F., Abe, K., Ogura, A
    • Journal Title

      Science

      Volume: 330 Issue: 6003 Pages: 496-499

    • DOI

      10.1126/science.1194174

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proviral silencing in embryonic stem cells requires the histone methyltrans-ferase ESET2010

    • Author(s)
      Matsui, T., Leung, D., Miyashita, H., Maksakova, I.A., Miyachi, H., Kimura, H., Tachibana, M., Lorincz, M.C., Shinkai, Y
    • Journal Title

      Nature

      Volume: 464 Issue: 7290 Pages: 927-931

    • DOI

      10.1038/nature08858

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET.2010

    • Author(s)
      Matsui, T., Leung, D., Miyashita, H., Maksakova, I.A., Miyachi, H., Kimura, H., Tachibana, M., Lorincz, M.C., Shinkai, Y.
    • Journal Title

      Nature 464

      Pages: 927-931

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation.2010

    • Author(s)
      Kanatsu-Shinohara, M., Takashima, S., Shinohara, T.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 107

      Pages: 6210-6215

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila2009

    • Author(s)
      Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., Kotani, H., Asai, K., Siomi, H., Siomi, MC
    • Journal Title

      Nature

      Volume: 461 Issue: 7268 Pages: 1296-1299

    • DOI

      10.1038/nature08501

    • Related Report
      2012 Final Research Report
  • [Journal Article] A signaling principle for the specification of the germ cell lineage in mice.2009

    • Author(s)
      Ohinata, Y., Ohta, H., Shigeta, M., Yamanaka, K., Wakayama, T., Saitou, M.
    • Journal Title

      Cell 137

      Pages: 398-400

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-Cyclin D2 activation.2009

    • Author(s)
      Lee, J., Kanatsu-Shinohara, M., Morimoto, H., Kazuki, Y., Takashima, S., Oshimura, M., Toyokuni, S., Shinohara, T
    • Journal Title

      Cell Stem Cell 5

      Pages: 76-86

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila.2009

    • Author(s)
      Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., Kotani, H., Asai, K., Siomi, H., Siomi. M.C.
    • Journal Title

      Nature 461

      Pages: 1296-1299

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.2009

    • Author(s)
      Shoji, M., Tanaka, T., Hosokawa, M., Reuter, M., Stark, A., Kato, Y., Kondoh, G., Okawa, K., Chujo, T., Suzuki, T., Hata, K., Martin, S.L., Noce, T., Kuramochi-Miyagawa, S., Nakano, T., Sasaki, H., Pillai, R.S., Nakatsuji, N., Chuma, S
    • Journal Title

      Dev. Cell 17

      Pages: 1-13

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes2008

    • Author(s)
      Watanabe, T., Totoki, Y., Toyoda, A., Kaneda, M., Kuramochi-Miyagawa, S., Obata, Y., Chiba, H., Kohara, Y., Kono, T., Nakano, T., Surani, M.A., Sakaki, Y., Sasaki, H
    • Journal Title

      Nature

      Volume: 453 Issue: 7194 Pages: 539-543

    • DOI

      10.1038/nature06908

    • Related Report
      2012 Final Research Report
  • [Journal Article] DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes.2008

    • Author(s)
      Kuramochi-Miyagawa, S., Watanabe, T., Gotoh, K., Totoki, Y., Toyoda, A., Ikawa, M., Asada, N., Kojima, K., Yamaguchi, Y., Ijiri, T.W., Hata, K., Li, E., Matsuda, Y., Kimura, T., Okabe, M., Sakaki, Y., Sasaki, H., Nakano, T.
    • Journal Title

      Genes Dev. 22

      Pages: 908-917

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes.2008

    • Author(s)
      Watanabe, T., Totoki, Y., Toyoda, A., Kaneda, M., Kuramochi-Miyagawa, S., Obata, Y., Chiba, H., Kohara, Y., Kono, T., Nakano, T., Surani, M.A., Sasaki, Y., Sasaki, H.
    • Journal Title

      Nature 453

      Pages: 539-543

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development.2008

    • Author(s)
      Hirasawa, R., Chiba, H., Kaneda, M., Tajima, S., Li, E., Jaenisch, R., Sasaki, H.
    • Journal Title

      Genes Dev. 22

      Pages: 1607-1616

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice.2008

    • Author(s)
      Kurimoto, K., Yabuta, Y., Ohinata, Y., Shigeta, M., Yamanaka, K., Saitou M.
    • Journal Title

      Genes Dev. 22

      Pages: 1617-1635

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice.2008

    • Author(s)
      Yamaji, M., Seki, Y., Kurimoto, K., Yabuta, Y., Yuasa, M., Shigeta, M, Yamanaka, K., Ohinata, Y., Saitou, M.
    • Journal Title

      Nat. Genet. 40

      Pages: 1016-1022

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends onβ1-integrin.2008

    • Author(s)
      Kanatsu-Shinohara, M., Takehashi, M., Takashima, S., Lee, J., Morimoto, H., Chuma, S., Raducanu, A., Nakatsuji, N., Fassler R., Shinohara, T.
    • Journal Title

      Cell Stem Cell 3

      Pages: 533-542

    • Related Report
      2009 Self-evaluation Report
  • [Book] A global single-cell cDNA amplification method for quantitative microarray analysis. In "PCR Protocols 3rd edition" Ed. by Danny Park (Humana Press, USA)2011

    • Author(s)
      Kurimoto, K. and Saitou, M
    • Publisher
      Methods in Molecular Biology
    • Related Report
      2012 Final Research Report
  • [Book] 「卵子学」(森 崇英・総編集)2011

    • Author(s)
      青木不学、小倉淳郎、河村和弘、斎藤通紀、河野友宏、佐々木裕之、田中智、松居靖久
    • Publisher
      京都大学学術出版会
    • Related Report
      2012 Final Research Report
  • [Book] Germline modification using mouse spermatogonial stem cells2010

    • Author(s)
      Kanatsu-Shinohara, M. and Shinohara, T
    • Publisher
      Methods Enzymol
    • Related Report
      2012 Final Research Report
  • [Book] Somatic cell nuclear transfer in the mouse. In "Microinjection: Methods and Applications Ed. by David J. Carroll (Humana Press, USA)2009

    • Author(s)
      Kishigami, S. and Wakayama, T
    • Publisher
      Methods in Molecular Biology
    • Related Report
      2012 Final Research Report
  • [Book] 「クローンマンモスへの道-クローン技術最前線の技術における発生・再生医療技術を探る 」(単著)2009

    • Author(s)
      若山照彦
    • Publisher
      アドスリー社
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.brc.riken.go.jp/lab/mcd/germline/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.brc.riken.go.jp/lab/mcd/germline/index.html#

    • Related Report
      2012 Annual Research Report
  • [Remarks] 領域ホームページ

    • URL

      http://www.brc.riken.go.jp/lab/mcd/germline/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks] 領域ホームページ

    • URL

      http://www.brc.riken.go.jp/lab/mcd/germline/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.brc.riken.go.jp/lab/mcd/germline/index.html

    • Related Report
      2009 Self-evaluation Report
  • [Remarks] 領域ホームページ

    • URL

      http://www.brc.riken.go.jp/lab/mcd/germline/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks] 領域ホームページ

    • URL

      http://www.brc.riken.go.jp/lab/mcd/germline/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks] 領域ホームページ

    • URL

      http://www.brc.riken.go.jp/lab/mcd/germline/index.html

    • Related Report
      2007 Annual Research Report

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Published: 2007-03-31   Modified: 2019-07-29  

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