Multi-dimensional fluorescent live imaging of cellular functions and molecular activities

• Project Area: Mutli-dimensional fluorescence live imaging of cellular function and molecular activity
• Project/Area Number: 22113001
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: MICHIYUKI Matsuda 京都大学, 生命科学研究科, 教授 (10199812)
• Co-Investigator(Kenkyū-buntansha): IMAMURA Takeshi 愛媛大学, 医学(系)研究科(研究院), 教授 (70264421) ; KIYOKAWA Etsuko 金沢医科大学, 医学部, 教授 (80300929)
• Co-Investigator(Renkei-kenkyūsha): MIYAWAKI Atsushi 独立行政法人理化学研究所, 細胞機能探索技術開発チーム, チームリーダー (80251445) ; NEMOTO Tomomi 北海道大学, 電子科学研究所, 教授 (50291084) ; OKADA Takaharu 独立行政法人理化学研究所, 免疫細胞動態研究ユニット, ユニットリーダー (50452272) ; ISHII Masaru 大阪大学, 大学院・生命機能研究科, 教授 (10324758) ; FUKUHARA Shigetomo 独立行政法人国立循環器病研究センター, 細胞生物学部, 室長 (70332880)
• Project Period (FY): 2010-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥114,400,000 (Direct Cost: ¥88,000,000、Indirect Cost: ¥26,400,000); Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2013: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2012: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2011: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2010: ¥33,670,000 (Direct Cost: ¥25,900,000、Indirect Cost: ¥7,770,000)
• Keywords: 蛍光イメージング / 蛍光顕微鏡 / 細胞生物学 / 蛍光タンパク質 / 蛍光プローブ / 細胞生物 / がん / 蛍光生体イメージング / 国際情報交換 / 国際研究者交流 / ライブイメージング / ケミカルバイオロジー / 生体イメージング / 二光子顕微鏡 / 超解像度イメージング / 緑色蛍光タンパク質 / FRET

Outline of Annual Research Achievements

平成26年度までの計画班員および公募班員の研究報告書を取りまとめ、編集作業を行った後、評価報告書を作成し、関係者に送付した。また、研究終了後も要望の強いDNAの配布や技術講習会も開催した。さらに、Web情報の更新も行った。平成27年度に新しい新学術領域研究「レゾナンスバイオ」が始まったので、この領域への情報の引き継ぎを行った。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse skin. (2015)
  • Author(s): Hiratsuka T, Fujita Y, Naoki H, Aoki K, Kamioka Y, Matsuda M
  • Journal Title: eLife Volume: - Pages: 1-18
  • DOI: 10.7554/elife.05178
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Circuit-dependent striatal PKA and ERK signaling underlies rapid behavioral shift in mating reaction of male mice. (2015)
  • Author(s): Goto A, Nakahara I, Yamaguchi T, Kamioka Y, Sumiyama K, Matsuda M, Nakanishi S, Funabiki K
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: １１２ Issue: 21 Pages: 6718-6723
  • DOI: 10.1073/pnas.1507121112
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Heterogeneity in ERK activity as visualized by in vivo FRET imaging or mammary tumor cells developed in MMTV-Neu mice. (2014)
  • Author(s): Yuka Kumagai, Honda Naoki, Eiji Nakasyo, Yuji Kamioka, Etsuko Kiyokawa, Michiyuki Matsuda
  • Journal Title: Oncogene Volume: (ネット上で先行掲載) Issue: 8 Pages: 1051-7
  • DOI: 10.1038/onc.2014.28
  • Peer Reviewed / Open Access
• [Journal Article] In vivo imaging reveals PKA regulation of ERK activity during neutrophil recruitment to inflamed intestines. (2014)
  • Author(s): Mizuno, R., Kamioka, Y., Kabashima, K., Imajo, M., Sumiyama, K., Nakasho, E., Ito, T., Hamazaki, Y., Okuchi, Y., Sakai, Y., Kiyokawa, E., and Matsuda, M.
  • Journal Title: J Exp Med Volume: 211 Issue: 6 Pages: 1123-36
  • DOI: 10.1084/jem.20132112
  • NAID: 120005439165
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chimaerin suppresses rac1 activation at the apical membrane to maintain the cyst structure (2012)
  • Author(s): Yagi, S., Matsuda, M., Kiyokawa, E.
  • Journal Title: Plos Ones Volume: 7 Issue: 12 Pages: e52258-e52258
  • DOI: 10.1371/journal.pone.0052258
  • Peer Reviewed
• [Journal Article] Activated ras protein accelerates cell cycle progression to perturb madin-darby canine kidney cystogenesis (2012)
  • Author(s): Yagi, S., Matsuda, M., Kiyokawa, E.
  • Journal Title: Journal of Biological Chemistry Volume: 38 Issue: 38 Pages: 31703-11
  • DOI: 10.1074/jbc.m112.377804
  • Peer Reviewed
• [Journal Article] Live Imaging of Protein Kinase Activities in Transgenic Mice Expressing FRET Biosensors (2012)
  • Author(s): Kamioka, Y., Sumiyama, K., Mizuno, R., Sakia, Y., Hirata, E., Kiyokawa, E., Matsuda, M.
  • Journal Title: Cell Structure and Function Volume: 37 Issue: 1 Pages: 65-73
  • DOI: 10.1247/csf.11045
  • NAID: 130004137578
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] Suppression of Rac1 Activity at the Apical Membrane of MDCK cells is Essential for Cyst Structure Maintenance (2012)
  • Author(s): Shunsuke Yagi, Michiyuki Matsuda, Etsuko Kiyokawa
  • Journal Title: EMBO Reports Volume: 13(3) Issue: 3 Pages: 237-243
  • DOI: 10.1038/embor.2011.249
  • Peer Reviewed
• [Journal Article] Phosphorylation of STEF/Tiam2 by protein kinase A is critical for Rac1 activation and neurite outgrowth in dibutyryl cAMP-treated PC12D cells (2011)
  • Author(s): Goto A, Hoshino M, Matsuda M, Nakamura T
  • Journal Title: Mol. Biol. Cell Volume: 22 Issue: 10 Pages: 1780-1792
  • DOI: 10.1091/mbc.e10-09-0783
  • Peer Reviewed
• [Journal Article] A genetically encoded Forster resonance energy transfer biosensor for two-photon excitation microscopy (2011)
  • Author(s): Y.Kumagai, Y.Kamioka, S.Yagi, M.Matsuda, E.Kiyokawa
  • Journal Title: Anal.Biochem. Volume: 413 Issue: 2 Pages: 192-199
  • DOI: 10.1016/j.ab.2011.02.021
  • Peer Reviewed
• [Journal Article] SH3BP1, an Exocyst-Associated RhoGAP, Inactivates Rac1 at the Front to Drive Cell Motility (2011)
  • Author(s): M.C.Parrini, A.Sadou-Dubourgnoux, K.Aoki, K.Kunida, M.Biondini, A.Hatzoglou, P.Poullet, E.Formstecher, C.Yeaman, M.Matsuda, C.Rosse, J.Camonis
  • Journal Title: Molecular Cell Volume: 42 Issue: 5 Pages: 650-661
  • DOI: 10.1016/j.molcel.2011.03.032
  • Peer Reviewed
• [Journal Article] The scaffold protein Shoc2/SUR-8 accelerates the interaction of Ras and Raf. (2010)
  • Author(s): Matsunaga-Udagawa R, Fujita Y, Yoshiki S, Terai K, Kamioka Y, Kiyokawa E, Yugi K, Aoki K, Matsuda M.
  • Journal Title: J Biol Chem. Volume: 285 Pages: 7818-7826
  • Peer Reviewed
• [Journal Article] Ras and Calcium Signaling Pathways Converge at Raf1 via the Shoc2 Scaffold Protein. (2010)
  • Author(s): Yoshiki S, Matsunaga-Udagawa R, Aoki K, Kamioka Y, Kiyokawa E, Matsuda M.
  • Journal Title: Mol Biol Cell. Volume: 15;21(6): Pages: 1088-1096
  • Peer Reviewed
• [Journal Article] Multiple decisive phosphorylation sites for the negative feedback regulation of SOS1 via ERK. (2010)
  • Author(s): Kamioka Y, Yasuda S, Fujita Y, Aoki K, Matsuda M.
  • Journal Title: J Biol Chem.19(6) Volume: 285 Pages: 33540-33548
  • Peer Reviewed
• [Presentation] Transgenic mouse lines with heritable and functional FRET biosensors (2015)
  • Author(s): Michiyuki Matsuda
  • Organizer: nternational Symposium on Multi-dimensional Fluorescence Live Imaging of Cellular Function and Molecular Activities
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2015-01-28
• [Presentation] Constitutively active mutation of Hras can be inhibited by cell contact interface (2015)
  • Author(s): Noriyuki Kawabata
  • Organizer: nternational Symposium on Multi-dimensional Fluorescence Live Imaging of Cellular Function and Molecular Activities
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2015-01-27
• [Presentation] Intravital Imaging of Signal Transduction Pathways (2013)
  • Author(s): Michiyuki Matsuda
  • Organizer: 「TGF-βファミリーシグナル国際共同研究拠点」第三回国際シンポジウム
  • Place of Presentation: 松山市 大和屋本店
  • Year and Date: 2013-10-29
• [Presentation] 管腔におけるアピカル膜でのRac1活性の抑制は上皮構造の形態維持に必要である (2011)
  • Author(s): 八木俊輔、松田道行、清川悦子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-01
• [Presentation] Live imaging of the mouse intestine by two-photon excitation microscopy (2011)
  • Author(s): Rei Mizuno, Etsuko Kiyokawa, Yoshiharu Sakai, Michiyuki Matsuda
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-04
• [Presentation] Live imaging of the mouse intestine by two-photon excitation microscopy (2011)
  • Author(s): Rei Mizuno, Etsuko Kiyokawa, Yoshiharu Sakai, Michiyuki Matsuda
  • Organizer: FASEB summer research conference Gastrointestinal tract XIV
  • Place of Presentation: The Steamboat Grand Resort(アメリカ)
  • Year and Date: 2011-08-17
• [Presentation] Visualisation of ERK kinase activity during tubulogenesis (2011)
  • Author(s): Kumagai Y, Matsuda M, Kiyokawa E
  • Organizer: Gordon Research Conference on Mammary Gland Biology 2011
  • Place of Presentation: Salve Regina University(アメリカ)
  • Year and Date: 2011-06-14
• [Presentation] Mechanism of Ras-induced polyp formation in vitro (2011)
  • Author(s): Atsuro Sakurai, Michiyuki Matsuda, Etsuko Kiyokawa
  • Organizer: FASEB summer research conference on Regulation and function of small GTPases
  • Place of Presentation: Vermont academy(アメリカ)
  • Year and Date: 2011-06-06
• [Remarks] 細胞機能と分子活性の多次元蛍光生体イメージング
  • URL: http://www.imaging.lif.kyoto-u.ac.jp/
• [Remarks] 細胞機能と分子活性の多次元イメージング
  • URL: http://www.imaging.lif.kyoto-u.ac.jp/papers/index.html