シリア・中心体系による生体情報フローの制御

• Project Area: Cilium-centrosome system regulating biosignal flows
• Project/Area Number: 24113001
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Research Institution: Osaka University
• Principal Investigator: 濱田 博司 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (00208589)
• Co-Investigator(Kenkyū-buntansha): 月田 早智子 大阪大学, 生命機能研究科, 教授 (00188517) ; 松崎 文雄 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (10173824) ; 稲垣 昌樹 愛知県がんセンター(研究所), その他部局等, その他(移行) (30183007) ; 五島 直樹 国立研究開発法人産業技術総合研究所, その他部局等, 研究員 (70215482) ; 広野 雅文 法政大学, 生命科学部, 教授 (10212177)
• Project Fiscal Year: 2012-06-28 – 2017-03-31
• Project Status: Granted(Fiscal Year 2016)
• Budget Amount: ¥105,430,000 (Direct Cost : ¥81,100,000、Indirect Cost : ¥24,330,000); Fiscal Year 2016 : ¥22,490,000 (Direct Cost : ¥17,300,000、Indirect Cost : ¥5,190,000); Fiscal Year 2015 : ¥18,720,000 (Direct Cost : ¥14,400,000、Indirect Cost : ¥4,320,000); Fiscal Year 2014 : ¥22,620,000 (Direct Cost : ¥17,400,000、Indirect Cost : ¥5,220,000); Fiscal Year 2013 : ¥18,720,000 (Direct Cost : ¥14,400,000、Indirect Cost : ¥4,320,000); Fiscal Year 2012 : ¥22,880,000 (Direct Cost : ¥17,600,000、Indirect Cost : ¥5,280,000)
• Keywords: 繊毛 / 中心体 / シグナル因子 / 細胞・組織 / 発現制御 / 発生・分化

Outline of Annual Research Achievements

H26年度は、総括班として以下のような活動を行った。
1)第3回領域会議を7月17日～18日に倉敷で開催し、計画研究員と公募研究員が参加して，研究の進展状況を発表しつつ、最新の情報を交換した。
2)第15回国際細胞膜研究フォーラム (iCeMS・京都大学）を、2015年3月2~4日に本新学術研究領域と共催で開催した。
3)電子顕微鏡観察、中心体・シリア関連の遺伝子リソースの確立・提供、変異マウスの作成・提供など、広い研究支援を行った。
4)本領域のHPを、情報交換のプラットフォームとして活用した。

Current Status of Research Progress

Research has progressed on the whole more than it was originally planned.

Reason

３年度目に行うべきことは、順調に進んだため。

Research Output

• [Journal Article] RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. (2015)
  • Author(s): Shiratsuchi, G., Takaoka, K., Ashikawa, T., Hamada H. and Kitagawa, D
  • Journal Title: EMBO J. Volume : 34 Pages : 97-114
  • DOI: 10.15252/emboj.201488979
• [Journal Article] Intestinal deletion of Claudin-7 enhances paracellular organic solute flux and initiates colonic inflammation in mice (2015)
  • Author(s): Tanaka H, Takechi M, Kiyonari H, Shioi G, Tamura A, Tsukita S.
  • Journal Title: Gut Volume : 印刷中 Pages : 印刷中
  • DOI: 10.1136/gutjnl-2014-308419
• [Journal Article] Tight junctions. Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin (2015)
  • Author(s): Saitoh Y, Suzuki H, Tani K, Nishikawa K, Irie K, Ogura Y, Tamura A, Tsukita S, Fujiyoshi Y.
  • Journal Title: Science Volume : 347 Pages : 775-778
  • DOI: 10.1126/science.1261833
• [Journal Article] Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm. (2014)
  • Author(s): Dong, F., Shinohara, K., Nabeshima, R., Botilde, Y., Asai, Y., Fukumoto, A., Hasegawa, T., Matsuo, M., Takeda, H., Shiratori, H., Nakamura T., and Hamada, H.
  • Journal Title: J. Cell Biol. Volume : 204 Pages : 203-213
  • DOI: 10.1083/jcb.201304076
• [Journal Article] Self-regulated left-right asymmetric expression of Pitx2c in the developing limb (2014)
  • Author(s): Shiratori, H., Yashiro, Iwai, N., Oki, S., Ikawa, Y., Kanata, K., and Hamada, H.
  • Journal Title: Dev. Biol. Volume : 395 Pages : 331-341
  • DOI: 10.1016/j.ydbio.2014.09.002
• [Journal Article] Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole (2014)
  • Author(s): Ohta M, Ashikawa T, Nozaki Y, Kozuka-Hata H, Goto H, Inagaki M, Oyama M, Kitagawa D.
  • Journal Title: Nat. Commun. Volume : 5 Pages : 5267
  • DOI: 10.1038/ncomms6267
• [Journal Article] Ubiquitin-proteasome system controls ciliogenesis at the initial step of axoneme extension (2014)
  • Author(s): Kasahara K, Kawakami Y, Kiyono T, Yonemura S, Kawamura Y, Era S, Matsuzaki F, Goshima N, Inagaki M.
  • Journal Title: Nat. Commun. Volume : 5 Pages : 5081
  • DOI: 10.1038/ncomms6081
• [Journal Article] Cdk1 coordinates timely activation of MKlp2 kinesin with relocation of the chromosome passenger complex for cytokinesis (2014)
  • Author(s): Kitagawa M, Fung SY, Hameed UF, Goto H, Inagaki M, Lee SH.
  • Journal Title: Cell Rep. Volume : 7 Pages : 166-179
  • DOI: 10.1016/j.celrep.2014.02.034
• [Journal Article] Hydrodynamic phase synchronization in mouse node cilia (2013)
  • Author(s): Takamatsu, A.*, Shinohara, K., Ishikawa, T. and Hamada, H.
  • Journal Title: Physical Review Letters. Volume : 110 Pages : 248107
• [Journal Article] Cluap1 localizes preferentially to the base and tip of cilia and is required for ciliogenesis in the mouse embryo (2013)
  • Author(s): Botilde, Y., Yoshiba, S., Shinohara, K., Hasegawa, T., Nishimura, H., Shiratori, H. and Hamada, H.
  • Journal Title: Developmental Biology Volume : 381 Pages : 203-212
  • DOI: 10.1016/j.ydbio.2013.05.024
• [Journal Article] Asymmetric rotational stroke in mouse node cilia during left-right determination. (2013)
  • Author(s): Takamatsu, A., Ishikawa, T., Shinohara, K., and Hamada, H.
  • Journal Title: Physical Review E. Volume : 87 Pages : 050701
• [Journal Article] The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK (2013)
  • Author(s): Yano T, Matsui T, Tamura A, Uji M, Tsukita S.
  • Journal Title: J Cell Biol. Volume : 203 Pages : 605-614
  • DOI: 10.1083/jcb.201304194
• [Journal Article] Two appendages homologous between basal bodies and centrioles are formed using distinct Odf2 domains (2013)
  • Author(s): Tateishi K, Yamazaki Y, Nishida T, Watanabe S, Kunimoto K, Ishikawa H, Tsukita S.
  • Journal Title: J Cell Biol. Volume : 203 Pages : 417-425
  • DOI: 10.1083/jcb.201303071
• [Journal Article] PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3γ and is required for metaphase-anaphase transition (2013)
  • Author(s): Kasahara K, Goto H, Izawa I, Kiyono T, Watanabe N, Elowe S, Nigg EA, Inagaki M.
  • Journal Title: Nat Commun. Volume : 4 Pages : 1882
  • DOI: 10.1038/ncomms2879
• [Presentation] Role of motile and immotile cilia in left-right asymmetry (2015)
  • Author(s): Hamada, H.
  • Organizer: Keystone Symposia (Heart Disease and Regeneration: Insights from Development)
  • Place of Presentation: Copper Mountain, Colorado, USA
  • Year and Date: 2015-03-01 – 2015-03-06
• [Presentation] Role of motile and immotile cilia in left-right symmetry breaking (2015)
  • Author(s): Hamada, H.
  • Organizer: Gordon Research Conference (CILIA, MUCUS & MUCOCILIARY INTERACTIONS)
  • Place of Presentation: Galveston, Texas, USA
  • Year and Date: 2015-02-08 – 2015-02-12
• [Presentation] Polarization of node cells by asymmetric Wnt5 distribution (2013)
  • Author(s): Hamada, H.
  • Organizer: OIST Conference (Gradients and Signaling)
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2013-11-11 – 2013-11-15
• [Presentation] Generating and sensing the flow signal for establishing left-right asymmetry (2013)
  • Author(s): Hamada, H.
  • Organizer: Annual Meeting of Korean Physiology Society
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2013-10-23 – 2013-10-25
• [Presentation] Multiple roles of cilia in left-right symmetry breaking (2013)
  • Author(s): Hamada, H.
  • Organizer: The 7th TAKAO Symposium
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2013-07-13 – 2013-07-15
• [Remarks] 新学術領域研究、シリア・中心体による生体情報フローの制御
  • URL: http://www.nig.ac.jp/labs/NigPrjct/cilia-centrosome/index.html
• [Remarks] 新学術領域研究、シリア・中心体系による生体情報フローの制御
  • URL: http://www.nig.ac.jp/labs/NigPrjct/cilia-centrosome/index.html