シリア・中心体系による生体情報フローの制御

• Project Area: Cilium-centrosome system regulating biosignal flows
• Project/Area Number: 24113001
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Research Institution: Institute of Physical and Chemical Research(2016); Osaka University(2012-2015)
• Principal Investigator: 濱田 博司 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (00208589)
• Co-Investigator(Kenkyū-buntansha): 月田 早智子 大阪大学, 生命機能研究科, 教授 (00188517) ; 松崎 文雄 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (10173824) ; 広野 雅文 法政大学, 生命科学部, 教授 (10212177) ; 稲垣 昌樹 愛知県がんセンター(研究所), その他部局等, その他(移行) (30183007) ; 五島 直樹 国立研究開発法人産業技術総合研究所, その他部局等, 研究員 (70215482) ; 岸本 健雄 お茶の水女子大学, サイエンスエデュケーションセンター, 客員教授 (00124222)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Project Status: Granted(Fiscal Year 2016)
• Budget Amount: ¥105,430,000 (Direct Cost : ¥81,100,000、Indirect Cost : ¥24,330,000); Fiscal Year 2016 : ¥22,490,000 (Direct Cost : ¥17,300,000、Indirect Cost : ¥5,190,000); Fiscal Year 2015 : ¥18,720,000 (Direct Cost : ¥14,400,000、Indirect Cost : ¥4,320,000); Fiscal Year 2014 : ¥22,620,000 (Direct Cost : ¥17,400,000、Indirect Cost : ¥5,220,000); Fiscal Year 2013 : ¥18,720,000 (Direct Cost : ¥14,400,000、Indirect Cost : ¥4,320,000); Fiscal Year 2012 : ¥22,880,000 (Direct Cost : ¥17,600,000、Indirect Cost : ¥5,280,000)
• Keywords: 繊毛 / 中心体 / シグナル因子 / 細胞・組織 / 発生制御 / 発生・分化 / 発現制御

Outline of Annual Research Achievements

1. 領域会議：第４回領域会議を8月27日～28日で倉敷シーサイドホテルで開催し、計画研究員と更新した公募研究員が参加し、研究の進展状況を発表しつつ、最新の情報を交換した。多くの共同研究が実施されている事が判った。2. 広報：日本生物物理学会（2015年9月13日～15日、金沢大学）において、池上班員と西坂班員が繊毛に関するシンポジウムを企画した。そのなかで、本新学術領域がDr.Charles Lindemann (Oakland University)を海外から招待し、共催講演をしてもらった。また、日本分子生物学会（2015年、12月1日～4日、神戸）で、班員が繊毛・中心体に関するワークショップを企画・実施した。また、H28年度の日本分子生物学会に対しても、繊毛・中心体に関するワークショップを企画・応募した。3. 技術支援：比較的小さな研究チームしかもたない公募班員を中心に、電子顕微鏡観察，中心体・シリア関連の遺伝子リソースの提供、変異マウスの作成・提供などの研究支援を行い、領域内の連携と共同研究を促進した。これまでの技術支援により、幾つかの共同研究の成果が発表された。
4. 国際シンポジウム：最終年度で開催予定の国際シンポジウムについて、日程や場所、海外招待者を協議・決定し、プログラムを確定した。また、この国際シンポジウムは、繊毛研究会と共同で開催することとした。

Current Status of Research Progress

2 :  Research has progressed on the whole more than it was originally planned.

Reason

４年目に行う予定だった集会・広報・技術支援活動が、順調に進んだため。

Strategy for Future Research Activity

1. 成果の発表：領域会議を開催し、情報交換・共同研究を促進する。最終年度に向けて、成果の発表につなげる。
2. 集会：海外の研究者を招待した国際シンポジウムを開催し，本領域研究の成果を発表しつつ、最新の情報を得る。
3. 技術支援：引き続き、電子顕微鏡・ゲノミクス解析・プロテオミクス解析・変異マウス作成・クラミドモナスのサンプル調製などの、技術支援を行う。
4. 広報：本領域研究のホームページや学会発表を通して、成果を社会へ発信する。昨年と同様に、日本分子生物学会・細胞生物学会などで、繊毛や中心体に関するワークショップを企画する。

Research Products

• [Int'l Joint Research] Oxford University/University of Birmingham/Cambridge University(イギリス)
  • Country Name: イギリス
  • Counterpart Institution: Oxford University/University of Birmingham/Cambridge University
• [Int'l Joint Research] University Medical Center Hamburg/University of Munster/Hohenheim University(ドイツ)
  • Country Name: ドイツ
  • Counterpart Institution: University Medical Center Hamburg/University of Munster/Hohenheim University
• [Int'l Joint Research] New York University/Emory University(アメリカ)
  • Country Name: アメリカ
  • Counterpart Institution: New York University/Emory University
• [Int'l Joint Research] Tel Aviv University(イスラエル)
  • Country Name: イスラエル
  • Counterpart Institution: Tel Aviv University
• [Int'l Joint Research] 北京工科大学(中国)
  • Country Name: 中国
  • Counterpart Institution: 北京工科大学
• [Int'l Joint Research]
  • # of Other Countries: 3
• [Journal Article] Cell cycle-independent transitions in temporal identity of mammalian neural progenitor cells (2016)
  • Author(s): Okamoto M, Miyata T, Konno D, Ueda HR, Kasukawa T, Hashimoto M, Matsuzaki F*, and Kawaguchi A* (corresponding authors)
  • Journal Title: Nat Commun. Volume : 印刷中 Pages : 印刷中
  • DOI: in press
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Claudin-21 Has a Paracellular Channel Role at Tight Junctions (2016)
  • Author(s): Tanaka H, Yamamoto Y, Kashihara H, Yamazaki Y, Tani K, Fujiyoshi Y, Mineta K, Takeuchi K, Tamura A, Tsukita S
  • Journal Title: Mol Cell Biol Volume : 36 Pages : 954-964
  • DOI: 10.1128/MCB.00758-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway (2016)
  • Author(s): Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M.
  • Journal Title: J Cell Biol Volume : 212 Pages : 409-423
  • DOI: 10.1083/jcb.201507046
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture (2016)
  • Author(s): Hilbert M*, Noga A*, Frey D*, Hamel V*, Guichard P, Kraatz SH, Pfreundschuh M, Hosner S, Flückiger I, Jaussi R, Wieser MM, Thieltges KM, Deupi X, Müller DJ, Kammerer RA, Gönczy P**, Hirono M**, Steinmetz MO**(**Corresponding authors)
  • Journal Title: Nat Cell Biol Volume : 18 Pages : 393-403
  • DOI: 10.1038/ncb3329
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Absence of radial spokes in mouse node cilia is required for rotational movement but confers ultrastructural instability as a trade-off. (2015)
  • Author(s): Shinohara, K., Chen, D., Nishida, T., Misaki, K., Yonemura, S. and Hamada, H.
  • Journal Title: Dev Cell Volume : 35 Pages : 236-246
  • DOI: 10.1016/j.devcel.2015.10.001
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. (2015)
  • Author(s): Shiratsuchi, G., Takaoka, K., Ashikawa, T., Hamada H. and Kitagawa, D
  • Journal Title: EMBO J. Volume : 34 Pages : 97-114
  • DOI: 10.15252/emboj.201488979
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Intestinal deletion of Claudin-7 enhances paracellular organic solute flux and initiates colonic inflammation in mice (2015)
  • Author(s): Tanaka H, Takechi M, Kiyonari H, Shioi G, Tamura A, Tsukita S.
  • Journal Title: Gut Volume : 印刷中 Pages : 印刷中
  • DOI: 10.1136/gutjnl-2014-308419
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tight junctions. Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin (2015)
  • Author(s): Saitoh Y, Suzuki H, Tani K, Nishikawa K, Irie K, Ogura Y, Tamura A, Tsukita S, Fujiyoshi Y.
  • Journal Title: Science Volume : 347 Pages : 775-778
  • DOI: 10.1126/science.1261833
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Self-regulated left-right asymmetric expression of Pitx2c in the developing limb (2014)
  • Author(s): Shiratori, H., Yashiro, Iwai, N., Oki, S., Ikawa, Y., Kanata, K., and Hamada, H.
  • Journal Title: Dev. Biol. Volume : 395 Pages : 331-341
  • DOI: 10.1016/j.ydbio.2014.09.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole (2014)
  • Author(s): Ohta M, Ashikawa T, Nozaki Y, Kozuka-Hata H, Goto H, Inagaki M, Oyama M, Kitagawa D.
  • Journal Title: Nat. Commun. Volume : 5 Pages : 5267
  • DOI: 10.1038/ncomms6267
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ubiquitin-proteasome system controls ciliogenesis at the initial step of axoneme extension (2014)
  • Author(s): Kasahara K, Kawakami Y, Kiyono T, Yonemura S, Kawamura Y, Era S, Matsuzaki F, Goshima N, Inagaki M.
  • Journal Title: Nat. Commun. Volume : 5 Pages : 5081
  • DOI: 10.1038/ncomms6081
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cdk1 coordinates timely activation of MKlp2 kinesin with relocation of the chromosome passenger complex for cytokinesis (2014)
  • Author(s): Kitagawa M, Fung SY, Hameed UF, Goto H, Inagaki M, Lee SH.
  • Journal Title: Cell Rep. Volume : 7 Pages : 166-179
  • DOI: 10.1016/j.celrep.2014.02.034
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm. (2014)
  • Author(s): Dong, F., Shinohara, K., Nabeshima, R., Botilde, Y., Asai, Y., Fukumoto, A., Hasegawa, T., Matsuo, M., Takeda, H., Shiratori, H., Nakamura T., and Hamada, H.
  • Journal Title: J. Cell Biol. Volume : 204 Pages : 203-213
  • DOI: 10.1083/jcb.201304076
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hydrodynamic phase synchronization in mouse node cilia (2013)
  • Author(s): Takamatsu, A.*, Shinohara, K., Ishikawa, T. and Hamada, H.
  • Journal Title: Physical Review Letters. Volume : 110 Pages : 248107
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cluap1 localizes preferentially to the base and tip of cilia and is required for ciliogenesis in the mouse embryo (2013)
  • Author(s): Botilde, Y., Yoshiba, S., Shinohara, K., Hasegawa, T., Nishimura, H., Shiratori, H. and Hamada, H.
  • Journal Title: Developmental Biology Volume : 381 Pages : 203-212
  • DOI: 10.1016/j.ydbio.2013.05.024
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Asymmetric rotational stroke in mouse node cilia during left-right determination. (2013)
  • Author(s): Takamatsu, A., Ishikawa, T., Shinohara, K., and Hamada, H.
  • Journal Title: Physical Review E. Volume : 87 Pages : 050701
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK (2013)
  • Author(s): Yano T, Matsui T, Tamura A, Uji M, Tsukita S.
  • Journal Title: J Cell Biol. Volume : 203 Pages : 605-614
  • DOI: 10.1083/jcb.201304194
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Two appendages homologous between basal bodies and centrioles are formed using distinct Odf2 domains (2013)
  • Author(s): Tateishi K, Yamazaki Y, Nishida T, Watanabe S, Kunimoto K, Ishikawa H, Tsukita S.
  • Journal Title: J Cell Biol. Volume : 203 Pages : 417-425
  • DOI: 10.1083/jcb.201303071
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3γ and is required for metaphase-anaphase transition (2013)
  • Author(s): Kasahara K, Goto H, Izawa I, Kiyono T, Watanabe N, Elowe S, Nigg EA, Inagaki M.
  • Journal Title: Nat Commun. Volume : 4 Pages : 1882
  • DOI: 10.1038/ncomms2879
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Cortical expansion during the development of mammalian complex brains (2015)
  • Author(s): Matsuzaki, F.
  • Organizer: 第３８回分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01 – 2015-12-04
  • Invited
• [Presentation] Role of maternal Nodal signaling in pre-mplantation development (2015)
  • Author(s): Hamada H.
  • Organizer: Mouse Molecular Genetics
  • Place of Presentation: Hinxton, UK
  • Year and Date: 2015-09-16 – 2015-09-19
  • Int'l Joint Research / Invited
• [Presentation] Role of tight junctions in biological systems (2015)
  • Author(s): Tsukita,S.
  • Organizer: Singapore Gastric Cancer Consortium 8th Annual Scientific Meeting
  • Place of Presentation: Tower Block, Singapore
  • Year and Date: 2015-07-22 – 2015-07-23
  • Int'l Joint Research / Invited
• [Presentation] Role of motile and immotile cilia in left-right asymmetry (2015)
  • Author(s): Hamada, H.
  • Organizer: Keystone Symposia (Heart Disease and Regeneration: Insights from Development)
  • Place of Presentation: Copper Mountain, Colorado, USA
  • Year and Date: 2015-03-01 – 2015-03-06
  • Invited
• [Presentation] Role of motile and immotile cilia in left-right symmetry breaking (2015)
  • Author(s): Hamada, H.
  • Organizer: Gordon Research Conference (CILIA, MUCUS & MUCOCILIARY INTERACTIONS)
  • Place of Presentation: Galveston, Texas, USA
  • Year and Date: 2015-02-08 – 2015-02-12
  • Invited
• [Presentation] Polarization of node cells by asymmetric Wnt5 distribution (2013)
  • Author(s): Hamada, H.
  • Organizer: OIST Conference (Gradients and Signaling)
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2013-11-11 – 2013-11-15
  • Invited
• [Presentation] Generating and sensing the flow signal for establishing left-right asymmetry (2013)
  • Author(s): Hamada, H.
  • Organizer: Annual Meeting of Korean Physiology Society
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2013-10-23 – 2013-10-25
  • Invited
• [Presentation] Multiple roles of cilia in left-right symmetry breaking (2013)
  • Author(s): Hamada, H.
  • Organizer: The 7th TAKAO Symposium
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2013-07-13 – 2013-07-15
  • Invited
• [Remarks] 個体パターニング研究チーム
  • URL: http://www.cdb.riken.jp/research/laboratory/hamada.html
• [Remarks] 月田研究室
  • URL: http://www.fbs.osaka-u.ac.jp/labs/tsukita/
• [Remarks] Cold Spring Harbor Perspectives in Biology
  • URL: http://cshperspectives.cshlp.org/search?fulltext=fumio+Matsuzaki&submit=yes&x=10&y=11
• [Remarks] 非対称細胞分裂研究チーム
  • URL: http://www.cdb.riken.jp/cas/
• [Remarks] 新学術領域研究、シリア・中心体系による生体情報フローの制御
  • URL: http://www.nig.ac.jp/labs/NigPrjct/cilia-centrosome/index.html
• [Remarks] 新学術領域研究、シリア・中心体による生体情報フローの制御
  • URL: http://www.nig.ac.jp/labs/NigPrjct/cilia-centrosome/index.html