| Budget Amount *help |
¥85,540,000 (Direct Cost: ¥65,800,000、Indirect Cost: ¥19,740,000)
Fiscal Year 2019: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2015: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
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| Outline of Final Research Achievements |
Three-dimensional morphogenesis of epithelial cells plays pivotal roles in many biological events as it is observed in embryogenesis. Mechanical properties of the extracellular substrate such as viscoelasticity and geometrical constraints are known as crucial factors that affect 3D morphogenesis of epithelial sheets. In this study, we found that epithelial cells (MDCK cells) cultured on a Matrigel crosslinked by Genipin exhibit dome formation by both the swelling of extracellular matrix and aquaporin water transport triggered by the basal hypertonic stress. Computer simulation was conducted and revealed that uneven swelling with a positive feedback between extending cell and enhanced water transport induces the dome formation. Moreover, we succeeded to establish a novel spherical substrate for cell culture by using a gelatin gel capsule. Human dermal fibroblasts cultured on the gel capsule made wrinkle-like structures on the spherical substrate, mimicking the early embryogenesis.
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