| Budget Amount *help |
¥81,250,000 (Direct Cost: ¥62,500,000、Indirect Cost: ¥18,750,000)
Fiscal Year 2019: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2018: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2017: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2015: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
|
|---|
| Outline of Final Research Achievements |
In order to estimate 3D stress distribution in Xenopus laevis embryos, we have established two methods, a sectioning method and a piercing method. In the sectioning method, an embryo is cut flat with a thin wire to measure the surface topography of the section along with the stiffness distribution on the surface. Stress distribution is estimated from these data with a finite element analysis as the stress distribution necessary to make the surface flat again. In the piercing method, a fine needle is pierced into an embryo and removed. Then, 3D shape of the pierced column is measured by serial sectioning of the frozen embryo. We have found in gastrula that the embryonic surface is in tension and embryonic core is in compression, and that the level of stresses changes during the process of development, and in tailbud that the notochord produces high compressive force which may cause tissue elongation.
|
