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Establishment of visualization of stress distribution in fresh embryos toward understanding of morphogenesis from biomechanical viewpoint

Planned Research

Project AreaDiscovery of the logic that establishes the 3D structure of organisms
Project/Area Number 15H05860
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University (2016-2019)
Nagoya Institute of Technology (2015)

Principal Investigator

Matsumoto Takeo  名古屋大学, 工学研究科, 教授 (30209639)

Co-Investigator(Kenkyū-buntansha) 田村 篤敬  鳥取大学, 工学研究科, 准教授 (30394836)
杉田 修啓  名古屋工業大学, 工学(系)研究科(研究院), 准教授 (20532104)
Project Period (FY) 2015-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥81,250,000 (Direct Cost: ¥62,500,000、Indirect Cost: ¥18,750,000)
Fiscal Year 2019: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2018: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2017: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2015: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Keywordsバイオメカニクス / 力学解析 / 細胞・組織 / 発生・形態形成 / 生物・生体工学 / 組織・細胞
Outline of Final Research Achievements

In order to estimate 3D stress distribution in Xenopus laevis embryos, we have established two methods, a sectioning method and a piercing method. In the sectioning method, an embryo is cut flat with a thin wire to measure the surface topography of the section along with the stiffness distribution on the surface. Stress distribution is estimated from these data with a finite element analysis as the stress distribution necessary to make the surface flat again. In the piercing method, a fine needle is pierced into an embryo and removed. Then, 3D shape of the pierced column is measured by serial sectioning of the frozen embryo. We have found in gastrula that the embryonic surface is in tension and embryonic core is in compression, and that the level of stresses changes during the process of development, and in tailbud that the notochord produces high compressive force which may cause tissue elongation.

Academic Significance and Societal Importance of the Research Achievements

発生現象に対する力学因子の影響は,近年その重要性が認識されはじめ,シート状の組織や胚の表面にレーザで穴を開けた際の周辺の組織変形の様子から平面内の力分布を求めることは盛んに行われるようになってきている.しかし,胚内部の3次元的な力分布については,計測方法の欠如から殆ど判っていなかった.そこで我々は,胚を切断したり,微細針を刺入したりした際の組織の3次元的な変形から内部の応力状態を推定する方法を考案し,その有効性を確認した.本法の普及により,胚内部の力分布が明らかとなり,発生に対する力学因子の影響がより深く理解できるようになると期待される.

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report

Research Products

(44 results)

All 2020 2019 2018 2017 2016 2015 Other

All Journal Article (18 results) (of which Peer Reviewed: 16 results,  Open Access: 8 results,  Acknowledgement Compliant: 7 results) Presentation (22 results) (of which Int'l Joint Research: 6 results,  Invited: 6 results) Book (1 results) Remarks (3 results)

  • [Journal Article] 細胞核への力学刺激と核内クロマチン分布2020

    • Author(s)
      松本健郎
    • Journal Title

      医学のあゆみ『ラミノパチーとはなにか』

      Volume: 272-2 Pages: 147-151

    • Related Report
      2019 Annual Research Report
  • [Journal Article] A novel FRET analysis method for tension dynamics in a single actin stress fiber: Application to MC3T3-E1 cells during movement on a substrate2019

    • Author(s)
      Wang Junfeng、Sugita Shukei、Michiue Tatsuo、Tsuboi Takashi、Kitaguchi Tetsuya、Matsumoto Takeo
    • Journal Title

      J Biorheol

      Volume: 33 Issue: 1 Pages: 21-26

    • DOI

      10.17106/jbr.33.21

    • NAID

      130007674886

    • ISSN
      1867-0466, 1867-0474
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shape-dependent regulation of differentiation lineages of bone marrow-derived cells under cyclic stretch2019

    • Author(s)
      Maeda Eijiro、Atsumi Yoshinori、Ishiguro Mai、Nagayama Kazuaki、Matsumoto Takeo
    • Journal Title

      Journal of Biomechanics

      Volume: 96 Pages: 109371-109371

    • DOI

      10.1016/j.jbiomech.2019.109371

    • NAID

      120006808764

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Heterogeneous Deformation in the Wall of Rabbit Thoracic Aorta at Microscopic Level2019

    • Author(s)
      Fan Y, Wang JF, Maeda E, Murase K, Matsumoto T
    • Journal Title

      ABE

      Volume: 8 Issue: 0 Pages: 17-13

    • DOI

      10.14326/abe.8.7

    • NAID

      130007552693

    • ISSN
      2187-5219
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photoelasticity-based evaluation of cellular contractile force for phenotypic discrimination of vascular smooth muscle cells2019

    • Author(s)
      Sugita Shukei, Mizutani Eri, Hozaki Masatoshi, Nakamura Masanori, Matsumoto Takeo
    • Journal Title

      Scientific Reports

      Volume: 9 Pages: 3960-3960

    • DOI

      10.1038/s41598-019-40578-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Direct application of mechanical stimulation to cell adhesion sites using a novel magnetic-driven micropillar substrate2018

    • Author(s)
      Nagayama K, Inoue T, Hamada Y, Sugita S, Matsumoto T
    • Journal Title

      Biomedical Microdevices

      Volume: 20 Pages: 85-85

    • DOI

      10.1007/s10544-018-0328-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of cyclic compression on the mechanical properties and calcification process of immature chick bone tissue in culture2017

    • Author(s)
      Maeda Eijiro、Nakagaki Masashi、Ichikawa Katsuhisa、Nagayama Kazuaki、Matsumoto Takeo
    • Journal Title

      Bone Reports

      Volume: 6 Pages: 120-128

    • DOI

      10.1016/j.bonr.2017.04.002

    • NAID

      120006331843

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Measurement of surface topography and stiffness distribution on cross-section of Xenopus laevis tailbud for estimation of mechanical environment in embryo2017

    • Author(s)
      Murakami Fumiaki、Ando Yoriko、Miyagi Asuka、Sugita Shukei、Ueno Naoto、Matsumoto Takeo
    • Journal Title

      Development, Growth & Differentiation

      Volume: 59 Pages: 434-443

    • DOI

      10.1111/dgd.12372

    • NAID

      120006331850

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel patterned magnetic micropillar array substrate for analysis of cellular mechanical responses2017

    • Author(s)
      Nagayama Kazuaki、Inoue Takuya、Hamada Yasuhiro、Matsumoto Takeo
    • Journal Title

      J Biomechanics

      Volume: 65 Pages: 194-202

    • DOI

      10.1016/j.jbiomech.2017.10.017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiphoton microscopy observations of 3D elastin and collagen fiber microstructure changes during pressurization in aortic media2017

    • Author(s)
      Sugita Shukei、Matsumoto Takeo
    • Journal Title

      Biomechanics and Modeling in Mechanobiology

      Volume: 16 Pages: 763-773

    • DOI

      10.1007/s10237-016-0851-9

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of actin filaments of MC3T3-E1 cells during adhesion process to substrate2016

    • Author(s)
      J Wang, S Sugita, K Nagayama, T Matsumoto
    • Journal Title

      JBSE

      Volume: 3 Issue: 2 Pages: 15-00637

    • DOI

      10.1299/jbse.15-00637

    • NAID

      130005158997

    • ISSN
      1880-9863
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Observations of intracellular tension dynamics of MC3T3-E1 cells during substrate adhesion using a FRET-based actinin tension sensor2016

    • Author(s)
      Wang J, Ito M, Zhong W, Sugita S, Michiue T, Tsuboi T, Kitaguchi T, Matsumoto T
    • Journal Title

      JBSE

      Volume: 11 Issue: 4 Pages: 16-00504

    • DOI

      10.1299/jbse.16-00504

    • NAID

      130005251141

    • ISSN
      1880-9863
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Differences in the Mechanical Properties of the Developing Cerebral Cortical Proliferative Zone between Mice and Ferrets at both the Tissue and Single-Cell Levels.2016

    • Author(s)
      Nagasaka, A., Shinoda, T., Kawaue, T., Suzuki, M., Nagayama, K., Matsumoto, T., Ueno, N., Kawaguchi, A. and Miyata, T.
    • Journal Title

      Front. Cell Dev. Biol

      Volume: 25 Pages: 139-139

    • DOI

      10.3389/fcell.2016.00139

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Multiphasic Stress Relaxation Response of Freshly Isolated and Cultured Vascular Smooth Muscle Cells Measured by Quasi-In Situ Tensile Test2015

    • Author(s)
      Nagayama K, Saito S, Matsumoto T
    • Journal Title

      Bio-Medical Materials and Engineering

      Volume: 25 Pages: 299-312

    • DOI

      10.3233/bme-151276

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mechanical trapping of the nucleus on micropillared surfaces inhibits the proliferation of vascular smooth muscle cells but not cervical cancer HeLa cells2015

    • Author(s)
      Nagayama K, Hamaji Y, Sato Y, Matsumoto T
    • Journal Title

      Journal of Biomechanics

      Volume: 48 Pages: 1796-1803

    • DOI

      10.1016/j.jbiomech.2015.05.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dynamic transport and cementation of skeletal elements build up pole-and-beam structured skeleton of sponges2015

    • Author(s)
      Nakayama S, Arima K, Kawai K, Mohri K, Inui C, Sugano W, Koba H, Tamada K, Nakata YJ, Kishimoto K, Arai-Shindo M, Kojima C, Matsumoto T, Fujimori T, Agata K, Funayama N
    • Journal Title

      Current Biology

      Volume: 25 Pages: 2549-54

    • DOI

      10.1016/j.cub.2015.08.023

    • NAID

      120005669373

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of Morphological Changes in Focal Adhesion during Cell Adhesion and Spreading on Glass Substrate2015

    • Author(s)
      王 軍鋒,杉田修啓,長山和亮,松本健郎
    • Journal Title

      TJSBME

      Volume: 53 Issue: 6 Pages: 311-318

    • DOI

      10.11239/jsmbe.53.311

    • NAID

      130005155604

    • ISSN
      1347-443X, 1881-4379
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 組織再生に向けた細胞のメカノトランスダクションの理解とその制御【依頼解説】2015

    • Author(s)
      長山和亮,松本健郎
    • Journal Title

      日本薬学会誌「ファルマシア」

      Volume: 51 Pages: 1066-1068

    • Related Report
      2015 Annual Research Report
  • [Presentation] 応答曲面法を用いたアフリカツメガエル原腸胚表層の力学特性の推定2020

    • Author(s)
      山本朋実,内藤大地,松本健郎,田村篤敬
    • Organizer
      日本機械学会中国四国支部第58期総会・講演会 (2020/3/6, 東広島)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Clearing of Xenopus laevis Tailbud Embryos and Quantification of their 3D-tissue Structure toward Finite Element Analysis2019

    • Author(s)
      Ando Y, Hayashi K, Maeda E, Tsunoda R, Tanaka H, Murase K, Ueno N, Matsumoto T
    • Organizer
      The 10th Asian-Pacific Conference on Biomechanics (2019/11/1-3, Taipei, Taiwan)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 押込試験によるアフリカツメガエル尾芽胚切断面かたさの多点計測装置の開発2019

    • Author(s)
      角田涼,安藤頼子,前田英次郎,上野直人,松本健郎
    • Organizer
      日本機械学会第32回バイオエンジニアリング講演会 (2019/12/20-21, 金沢)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 数値モデルを用いたアフリカツメガエル原腸胚表層の力学特性の推定2019

    • Author(s)
      山本朋実,才木美香,田村篤敬,松本健郎
    • Organizer
      日本機械学会第32回バイオエンジニアリング講演会 (2019/12/20-21, 金沢)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Biomechanical approaches toward elucidation of mechanical environment in Xenopus laevis embryos2018

    • Author(s)
      Matsumoto T
    • Organizer
      Japan-Toronto Morphogenesis Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Macroscopic and microscopic heterogeneities in the aortic wall: Close correlation between biomechanical environment and protein expression2018

    • Author(s)
      Matsumoto T
    • Organizer
      21st International Conference on Mechanics in Medicine and Biology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] アフリカツメガエル新鮮原腸胚内部の構造観察と力学環境の推定2018

    • Author(s)
      丹下祥之,安東頼子,前田英次郎,村瀬晃平,上野直人,松本健郎
    • Organizer
      第41回日本バイオレオロジー学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 粒子散布法によるアフリカツメガエル尾芽胚のかたさ計測2018

    • Author(s)
      角田 涼,安東頼子,前田英次郎,村瀬晃平,上野直人,松本健郎
    • Organizer
      第41回日本バイオレオロジー学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 刺入法によるアフリカツメガエル新鮮胚内部の力学環境の推定2018

    • Author(s)
      丹下祥之,安東頼子,前田英次郎,村瀬晃平,上野直人,松本健郎
    • Organizer
      日本機械学会第31回バイオエンジニアリング講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アフリカツメガエル尾芽胚切断面かたさの同時多点計測2018

    • Author(s)
      角田 涼,安東頼子,上野直人,前田英次郎,村瀬晃平,松本健郎
    • Organizer
      日本機械学会東海支部第49回学生員卒業研究発表講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アフリカツメガエル新鮮原腸胚内部の力学環境の推定に関する研究2018

    • Author(s)
      丹下祥之,安東頼子,前田英次郎,村瀬晃平,上野直人,松本健郎
    • Organizer
      日本機械学会東海支部第67期総会・講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Biomechanical Approaches toward Estimation of Stress Distribution in Xenopus Laevis Embryos2017

    • Author(s)
      Matsumoto T
    • Organizer
      Japan-UCI Meeting on 3D Morphogenesis
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Estimation of shear deformation of glycocalyx layer on vascular endothelial cells in response to fluid flow2017

    • Author(s)
      Matsumoto T, Takahashi Y, Owaki Y, Nagayama K
    • Organizer
      The Third International Symposium on Mechanobiology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 刺入法によるアフリカツメガエル新鮮原腸胚内部の力学環境の推定に関する研究2017

    • Author(s)
      丹下祥之,前田英次郎,村瀬晃平,上野直人,松本健郎
    • Organizer
      第40回日本バイオレオロジー学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アフリカツメガエル胚内部の3次元応力分布の推定2017

    • Author(s)
      松本健郎
    • Organizer
      日本機械学会第29回バイオエンジニアリング講演会
    • Related Report
      2016 Annual Research Report
  • [Presentation] FRETに基づくアクチニン張力センサを用いた基板接着過程でのMC3T3-E1細胞内張力のダイナミクスの観察2017

    • Author(s)
      王軍鋒,伊藤将大,鐘文浩,杉田修啓,道上達男,坪井貴司,北口哲也,松本健郎
    • Organizer
      日本機械学会第29回バイオエンジニアリング講演会
    • Related Report
      2016 Annual Research Report
  • [Presentation] アフリカツメガエル新鮮原腸胚内部の張力分布異方性の計測に関する研究2017

    • Author(s)
      丹下祥之,前田英次郎,村瀬晃平,上野直人,松本健郎
    • Organizer
      日本機械学会東海支部第48回学生員卒業研究発表講演会
    • Related Report
      2016 Annual Research Report
  • [Presentation] り鉢様形状の培養基板面が細胞集団の挙動に与える影響の観察2017

    • Author(s)
      金森宗一郎,前田英次郎,村瀬晃平,松本健郎
    • Organizer
      日本機械学会東海支部第48回学生員卒業研究発表講演会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 胚断面高さおよびかたさ分布計測によるアフリカツメガエル脊索形成時の力学場の推定2016

    • Author(s)
      村上史哲,宮城明日香,杉田修啓,上野直人,松本健郎
    • Organizer
      日本機械学会第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京工業大学,東京都
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体組織内力分布を細胞の寸法レベルで明らかにする2016

    • Author(s)
      松本健郎
    • Organizer
      基生研研究会『物理学は生物現象の謎を解けるか』
    • Place of Presentation
      基礎生物学研究所,愛知県岡崎市
    • Year and Date
      2016-01-05
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Estimation of stress distribution in developing Xenopus tail bud2016

    • Author(s)
      Matsumoto T
    • Organizer
      Japan-Austria joint meeting “Understanding the logic behind developmental dynamics”
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] アフリカツメガエル新鮮胚断面かたさ分布瞬時計測法の高精度化に関する研究2016

    • Author(s)
      加藤雅也,宮城明日香,杉田修啓,上野直人,松本健郎
    • Organizer
      日本機械学会東海支部第47回学生員卒業研究発表講演会
    • Place of Presentation
      愛知工業大学,愛知県八草町
    • Related Report
      2015 Annual Research Report
  • [Book] Advances in Metallic Biomaterials: Tissues, Materials and Biological Reactions (Niinomi M, Narushima T, Nakai M, eds.)2015

    • Author(s)
      Matsumoto T, Sugita S, Yaguchi T
    • Total Pages
      348
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Remarks] 名古屋大学・バイオメカニクス研究室

    • URL

      http://bio.mech.nagoya-u.ac.jp/

    • Related Report
      2019 Annual Research Report
  • [Remarks] バイオメカニクス研究室

    • URL

      http://bio.mech.nagoya-u.ac.jp/

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] 名古屋工業大学 大学院工学研究科 機能工学専攻 バイオメカニクス研究室

    • URL

      http://biomech.web.nitech.ac.jp/Previous/top.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-07-02   Modified: 2022-11-04  

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