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膜の疎水領域でのリポクオリティ認識機構とナノ膜ドメインの解明

Planned

Project AreaQuality of lipids in biological systems
Project/Area Number 15H05901
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

岡村 康司  大阪大学, 医学系研究科, 教授 (80201987)

Co-Investigator(Kenkyū-buntansha) 中川 敦史  大阪大学, 蛋白質研究所, 教授 (20188890)
Project Period (FY) 2015-06-29 – 2020-03-31
Project Status Granted (Fiscal Year 2019)
Budget Amount *help
¥95,290,000 (Direct Cost: ¥73,300,000、Indirect Cost: ¥21,990,000)
Fiscal Year 2019: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2018: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2017: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2016: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Keywords脂質 / 蛋白質 / シグナル伝達 / 酵素 / 生理学 / イオンチャネル / タンパク質ドメイン / 電位センサー / イノシトールリン脂質
Outline of Annual Research Achievements

哺乳類培養細胞でのイノシトールリン脂質の動態を解析する効果的な分子ツールとしてこれまでに世界的に用いられてきた脊椎動物ゼブラフィッシュ由来Voltage-sensing phosphatase(Dr-VSP)について、前年度までにホヤ由来VSP(Ci-VSP)での詳細な解析から見出したホスファターゼドメイン中のhydrophobic spineの膜脂質との相互作用候補アミノ酸部位を改変することにより、電位依存性酵素活性を向上させることによる分子ツールの改良が可能かを、主に発現系細胞HEK293T細胞での電気生理学的解析、蛍光顕微鏡をもちいた発現解析によって検討した。
これまでの脂質のリポクオリティを認識する分子機序としてCi-VSPのhydrophobic spineのL284に対応するL223をFに変異させた変異体は電位依存的酵素活性を1.7倍増加させた(Kir2.1活性での評価)。更に細胞膜への限局を向上させるため、これまでの解析から細胞膜へのターゲットを向上させることが知られていたCi-VSPのN末端側細胞内領域とのキメラを作成した(CiDr-VSP-mChe-L223F)(表面での限局を確認するため蛍光タンパク質も融合させた)。これにより野生型のDr-VSPよりも2.1倍から2.8倍に電位依存的酵素活性を向上させることに成功した。また、研究分担者の中川のチームによる解析によって、様々なイノシトールリン脂質を基質としたVSPの共結晶を作製し高分解能での構造情報を得ることに成功した。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

これまでに明らかにしてきたVSPの膜との相互作用部位hydrophobic spineが、ホヤ由来VSPだけでなく脊椎動物のVSPについても保存されていることが明らかになり、さらにこの部位のアミノ酸変異により、イノシトールリン脂質のレベルを短時間で変化させる分子ツールとしての性質を向上させることに成功した。VSPのX線結晶解析についても、基質との共結晶が複数得られており、結合部位の構造基盤が明らかになりつつある。

Strategy for Future Research Activity

VSPについてはリポクオリティ認識機構の原理を深く知るため、Ci-VSPについて、これまでに確立してきた発現系細胞での非天然アミノ酸ANAPを用いたVoltage clamp fluorometry法を更に工夫し、側鎖間での相互作用を解析する。これまでに電位依存的なスペクトルの変化の計測が可能であったH182Anapのコンストラクトを用いて、更に解像度の高いシグナルを得られるかを検討する。またこのシグナルの分子機構について、他の部位に2つ目の変異を導入するなどによる解析をおこなって明らかにする。VSPの構造については、これまでに得てきた様々な基質との共結晶構造について更に分解能を上げられるかを検討する(研究分担者中川)。

Report

(4 results)
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report

Research Products

(157 results)

All 2020 2019 2018 2017 2016 2015

All Journal Article (36 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 32 results,  Open Access: 17 results,  Acknowledgement Compliant: 9 results) Presentation (116 results) (of which Int'l Joint Research: 36 results,  Invited: 33 results) Book (5 results)

  • [Journal Article] The Slo3/Lrrc52 complex is sensitive to phosphoinotsitides.2020

    • Author(s)
      Kawai T, Okamura Y.
    • Journal Title

      Channels

      Volume: -

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Hv1/VSOP regulates neutrophil directional migration and ERK activity by tuning ROS production.2020

    • Author(s)
      Okochi Y, Umemoto E, Okamura Y.
    • Journal Title

      J Leukoc Biol .

      Volume: 107 Pages: 819-831

    • DOI

      10.1002/jlb.2a0320-110rr

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activation Mechanisms of Voltage Sensing Phosphatase2020

    • Author(s)
      川鍋 陽. 西澤和久. 岡村康司
    • Journal Title

      Biophysics

      Volume: 60 Issue: 2 Pages: 105-107

    • DOI

      10.2142/biophys.60.105

    • NAID

      130007818361

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Engineering an enhanced voltage-sensing phosphatase2020

    • Author(s)
      Kawanabe Akira、Mizutani Natsuki、Polat Onur K.、Yonezawa Tomoko、Kawai Takafumi、Mori Masayuki X.、Okamura Yasushi
    • Journal Title

      Journal of General Physiology

      Volume: 152

    • DOI

      10.1085/jgp.201912491

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polarized PtdIns(4,5)P2distribution mediated by a voltage-sensing phosphatase (VSP) regulates sperm motility2019

    • Author(s)
      Kawai Takafumi、Miyata Haruhiko、Nakanishi Hiroki、Sakata Souhei、Morioka Shin、Sasaki Junko、Watanabe Masahiko、Sakimura Kenji、Fujimoto Toyoshi、Sasaki Takehiko、Ikawa Masahito、Okamura Yasushi
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 116 Pages: 26020-26026

    • DOI

      10.1073/pnas.1916867116

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional analysis of a double-point mutation in the KCNJ2 gene identified in a family with Andersen-Tawil syndrome2019

    • Author(s)
      Fukumura Shinobu、Yamauchi Kosuke、Kawanabe Akira、Yamamoto Akiyo、Nakaza Maki、Kubota Tomoya、Kato Shinsuke、Sasaki Ryogen、Okamura Yasushi、Takahashi Masanori P.
    • Journal Title

      Journal of the Neurological Sciences.

      Volume: 407 Pages: 116521-116521

    • DOI

      10.1016/j.jns.2019.116521

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct functional properties of two electrogenic isoforms of the SLC 34 Na‐Pi cotransporter2019

    • Author(s)
      Mizutani Natsuki、Okochi Yoshifumi、Okamura Yasushi
    • Journal Title

      Physiol. Reports

      Volume: 7

    • DOI

      10.14814/phy2.14156

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ホスフォイノシチドによるチャネル・トランスポーターの制御2019

    • Author(s)
      岡村康司、水谷夏希、大河内善史
    • Journal Title

      医学のあゆみ

      Volume: 269 Pages: 1108-1113

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Real-time functional analysis of Hv1 channel in neutrophils: a new approach from zebrafish model2019

    • Author(s)
      Ratanayotha Adisorn、Kawai Takafumi、Okamura Yasushi
    • Journal Title

      American Journal of Physiology

      Volume: 316 Pages: 819-831

    • DOI

      10.1152/ajpregu.00326.2018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of coupling to voltage sensors in voltage-evoked cellular signals2019

    • Author(s)
      OKAMURA Yasushi、OKOCHI Yoshifumi
    • Journal Title

      Proc. Jpn. Acad., Ser. B

      Volume: 95 Issue: 3 Pages: 111-135

    • DOI

      10.2183/pjab.95.010

    • NAID

      130007610041

    • ISSN
      0386-2208, 1349-2896
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dynamic structural rearrangements and functional regulation of voltage‐sensing phosphatase2019

    • Author(s)
      Sakata S and Okamura Y
    • Journal Title

      The Journal of Physiology

      Volume: 597 Pages: 29-40

    • DOI

      10.1113/jp274113

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The hydrophobic nature of a novel membrane interface regulates the enzyme activity of a voltage-sensing phosphatase2018

    • Author(s)
      Kawanabe A, Hashimoto M, Mishizawa M, Nishizawa N, Narita H, Yonezawa T, Jinno Y, Sakata S, Nakawaga A and Okamura Y
    • Journal Title

      eLife

      Volume: 7 Pages: 2097-2131

    • DOI

      10.7554/elife.41653

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Zn2+-Binding to the Voltage-Gated Proton Channel Hv1/VSOP2018

    • Author(s)
      Iwaki Masayo、Takeshita Kohei、Kondo Hiroko X.、Kinoshita Kengo、Okamura Yasushi、Takano Yu、Nakagawa Atsushi、Kandori Hideki
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 122 Pages: 9076-9080

    • DOI

      10.1021/acs.jpcb.8b04890

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tracking the sarcoplasmic reticulum membrane voltage in muscle with a FRET biosensor2018

    • Author(s)
      Sanchez Colline、Berthier Christine、Allard Bruno、Perrot Jimmy、Bouvard Cl?ment、Tsutsui Hidekazu、Okamura Yasushi、Jacquemond Vincent
    • Journal Title

      The Journal of General Physiology

      Volume: 150 Pages: 1163-1177

    • DOI

      10.1085/jgp.201812035

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Conservation of the Ca2+-permeability through the voltage sensor domain of mammalian CatSper subunit2018

    • Author(s)
      Arima Hiroki、Tsutsui Hidekazu、Okamura Yasushi
    • Journal Title

      Channels

      Volume: 12 Pages: 240-248

    • DOI

      10.1080/19336950.2018.1476791

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Induction of divalent cation permeability by heterologous expression of a voltage sensor domain2018

    • Author(s)
      Arima Hiroki、Tsutsui Hidekazu、Sakamoto Ayako、Yoshida Manabu、Okamura Yasushi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Biomembranes

      Volume: 1860 Pages: 981-990

    • DOI

      10.1016/j.bbamem.2018.01.004

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mechanistic insight into the suppression of microglial ROS production by voltage-gated proton channels(VSOP/Hv1)2018

    • Author(s)
      Kawai T, Tatsumi S, Kihara S, Sakimura K, Okamura Y.
    • Journal Title

      Channels

      Volume: 12 Issue: 1 Pages: 43108-43108

    • DOI

      10.1080/19336950.2017.1385684

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Voltage-Sensing Phosphatases: Biophysics, Physiology, and Molecular Engineering2018

    • Author(s)
      Okamura Yasushi、Kawanabe Akira、Kawai Takafumi
    • Journal Title

      Physiological Reviews

      Volume: 98 Pages: 2097-2131

    • DOI

      10.1152/physrev.00056.2017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Evolutionary History of Voltage-Gated Sodium Channels.2018

    • Author(s)
      Nishino Atsuo, Okamura Yasushi
    • Journal Title

      Handbook of Experimental Pharmacology, Springer

      Volume: Epub ahead of print Pages: 1-30

    • DOI

      10.1007/164_2017_70

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脂質-イオンチャネル相互連関2018

    • Author(s)
      岡村康司、大澤匡範
    • Journal Title

      「脂質クオリティ」実験医学増刊

      Volume: vol.36, No.10 Pages: 97-104

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unconventional role of voltage-gated proton channels (VSOP/Hv1) in regulation of microglial ROS production.2017

    • Author(s)
      Kawai T, Okochi Y, Ozaki T, Imura Y, Koizumi S, Yamazaki M, Abe M, Sakimura K, Yamashita T, Okamura Y.
    • Journal Title

      J Neurochem.

      Volume: 142 Issue: 5 Pages: 686-699

    • DOI

      10.1111/jnc.14106

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular and functional characterization of the voltage-gated proton channel in zebrafish neutrophils2017

    • Author(s)
      Ratanayotha Adisorn、Kawai Takafumi、Higashijima Shin-ichi、Okamura Yasushi
    • Journal Title

      Physiol Rep

      Volume: 5

    • DOI

      10.14814/phy2.13345

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 電位センサードメインを用いた膜電位プローブの進歩2017

    • Author(s)
      河合 喬文、筒井 秀和、岡村 康司
    • Journal Title

      生体の科学

      Volume: 68 Issue: 5 Pages: 444-445

    • DOI

      10.11477/mf.2425200686

    • ISSN
      0370-9531, 1883-5503
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate.2017

    • Author(s)
      Shinobu Hirai, Kohji Hotta, Yoshihiro Kubo, Atsuo Nishino, Shigeo Okabe, Yasushi Okamura, Haruo Okado
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: - Pages: 3939-3944

    • DOI

      10.1073/pnas.1612943114

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging.2017

    • Author(s)
      Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda M, and Nagai T
    • Journal Title

      Scientific Report

      Volume: 7 Pages: 42398-42398

    • DOI

      10.1038/srep42398

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Comparison between mouse and sea urchin orthologs of voltage-gated proton channel suggests role of S3 segment in activation gating2016

    • Author(s)
      Sakata S, Miyawaki N, McCormack TJ, Arima H, Kawanabe A, Ozkucur N, Kurokawa T, Jinno Y, Fujiwara Y, Okamura Y
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1858 Pages: 2972-2983

    • DOI

      10.1016/j.bbamem.2016.09.008

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Voltage-dependent motion of the catalytic region of voltage-sensing phosphatase monitored by a fluorescent amino acid2016

    • Author(s)
      Sakata S, Jinno Y, Kawanabe A, Okamura Y
    • Journal Title

      Proc Natil Acad Sci USA

      Volume: 113 Pages: 7521-7526

    • DOI

      10.1073/pnas.1604218113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural basis for the membrane association of ankyrinG via palmitoylation2016

    • Author(s)
      Fujiwara Y, Kondo H, Shirota M, Kobayashi M, Takeshita K, Nakagawa A, Okamura Y, Kinoshita K
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 23981-23981

    • DOI

      10.1038/srep23981

    • NAID

      120006770875

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Recent Views of Proton-Selective Permeation: Lesson from Voltage-Gated Proton Channel2016

    • Author(s)
      岡村 康司
    • Journal Title

      Biophysics

      Volume: 56 Issue: 3 Pages: 154-158

    • DOI

      10.2142/biophys.56.154

    • NAID

      130005153075

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simple scheme of lipid enzyme can explain complex lives of phosphoinositides.2016

    • Author(s)
      Okamura Y.
    • Journal Title

      PNAS

      Volume: 113 Pages: 7012-7014

    • DOI

      10.1073/pnas.1607427113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] SPring-8 BL44XU, beamline designed for structure analysis of large biological macromolecular assemblies2016

    • Author(s)
      Akifumi Higashiura, Eiki Yamashita, Masato Yoshimura, Kazuya Hasegawa, Yukito Furukawa, Takashi, Kumasaka, Go Ueno, Masaki Yamamoto, Tomitake Tsukihara and Atsushi Nakagawa
    • Journal Title

      AIP Conference Proceedings

      Volume: 1741

    • Related Report
      2016 Annual Research Report
  • [Journal Article] The voltage-gated proton channel Hv1/VSOP inhibits neutrophil granule release2016

    • Author(s)
      Okochi Y, Aratani Y, Adissu HA, Miyawaki N, Sasaki M, Suzuki K, Okamura Y.
    • Journal Title

      J Microbiol Methods

      Volume: 99 Pages: 159-163

    • DOI

      10.1189/jlb.3hi0814-393r

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effects of unsaturated fatty acids on the kinetics of voltage-gated proton channels heterologously expressed in cultured cells.2016

    • Author(s)
      Kawanabe A., Okamura Y.
    • Journal Title

      J. Physiol.

      Volume: 594 Pages: 595-610

    • DOI

      10.1113/jp271274

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Direct Interaction between the Voltage-sensors Produces Cooperative Sustained Deactivation in Voltage-gated H+ Channel Dimers.2016

    • Author(s)
      Okuda H, Yonezawa Y, Takano Y, Okamura Y, Fujiwara Y.
    • Journal Title

      J BIOL CHEM

      Volume: 291 Pages: 5935-47

    • DOI

      10.1074/jbc.m115.666834

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural insight into the regulation mechanism of proton leakage based on crystal structure of voltage-gated proton channel (VSOP)2015

    • Author(s)
      竹下 浩平,岡村 康司,中川 敦史
    • Journal Title

      生化学

      Volume: 87-5 Issue: 5 Pages: 625-628

    • DOI

      10.14952/SEIKAGAKU.2015.870625

    • NAID

      40020650286

    • ISSN
      0037-1017
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gating mechanisms of voltage-gated proton channels.2015

    • Author(s)
      Okamura Y, Fujiwara Y, Sakata S.
    • Journal Title

      Annual Rev. Biochem.

      Volume: 84 Pages: 685-709

    • DOI

      10.1146/annurev-biochem-060614-034307

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] The conformational change of the cytoplasmic region of voltage-sensing phosphatase2020

    • Author(s)
      Akira Kawanabe,Manami Nishizawa,Kazuhisa Nishizawa,Hirotaka Narita,Tomoko Yonezawa,Yuka Jinno,Souhei Sakata,Atsushi Nakagawa,Yasushi Okamura.
    • Organizer
      the 97th Annual Meeting of the Physiological Society of Japan(第97回日本生理学会大会)誌上開催
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of phosphoinositide distribution by voltage-sensing phosphatase in mouse spermatozoa2020

    • Author(s)
      Takafumi Kawai,Yasushi Okamura
    • Organizer
      the 97th Annual Meeting of the Physiological Society of Japan(第97回日本生理学会大会)誌上開催
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analyzing PI(4,5)P2 sensitivity of GABAA receptor by voltage sensing phosphatase2020

    • Author(s)
      Shunich Sugimoto,Takafumi Kawai,Yasushi Okamura
    • Organizer
      the 97th Annual Meeting of the Physiological Society of Japan(第97回日本生理学会大会)誌上開催
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Alteration in the coupling between voltage sensor movement and phosphatase activity in voltage-sensing phosphatase with mutation in voltage sensor domani2020

    • Author(s)
      Natsuki Mizutani, Yasushi Okamura
    • Organizer
      the 97th Annual Meeting of the Physiological Society of Japan(第97回日本生理学会大会)誌上開催
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment of a method measuring membrane potential in phagosomes2020

    • Author(s)
      Yoshifumi Okochi,Hidekazu Tsutsui,Yasushi Okamura
    • Organizer
      the 97th Annual Meeting of the Physiological Society of Japan(第97回日本生理学会大会)誌上開催
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Coupling Mechanisms of VSD Mutants of CI-VSP2020

    • Author(s)
      Natsuki Mizutani,Yasushi Okamura
    • Organizer
      64th Biophysical Society Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス精子に存在する電位依存性ホスファターゼの機能2019

    • Author(s)
      河合喬文、岡村康司
    • Organizer
      第112回近畿生理学談話会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Regulation of ion channels by distribution of phospholipids in mouse sperm2019

    • Author(s)
      Yasushi Okamura
    • Organizer
      Ion Channel Modulation Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Improvement of voltage-sensing phosphatase as a molecular tool for phosphoinositide depletion in living cells2019

    • Author(s)
      Akira kawanabe, Natsuki Mizutani, Tomoko Yonezawa, Yasushi Okamura
    • Organizer
      The 57th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evolutional insight into the orign of clustering of voltage-gated sodium channels2019

    • Author(s)
      Yasushi Okamura,Yuka Jinno, Masaki Hashimoto,Junko Nishino,Yasunori Sasakura,Atsuo Nishino,Tamafumi Kawai
    • Organizer
      NEURO2019(第42回日本神経科学大会、第62回日本神経化学会大会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of membrane potential of phagosome in phagocytes2019

    • Author(s)
      Yoshifumi Okochi,Hidekazu Tsutsui,Yasushi Okamura
    • Organizer
      60th International Conference on the Bioscience of Lipids(ICBL2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Function of voltage-sensing phosphatase in mouse sperm2019

    • Author(s)
      Takahumi Kawai,Haruhiko Miyata, Hiroko Nakanishi,Souhei Sakata,Yoshifumi Okochi,Masahiko Watanabe,Toyoshi Fujimoto,Kenji Sakimura, Takehiko Sasaki,Masahito Ikawa,Yasushi Okamura
    • Organizer
      60th International Conference on the Bioscience of Lipids(ICBL2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The hydrophobic protein-membrane interface maintains an activity of a phosphoinositide phosphatase2019

    • Author(s)
      Akira Kawanabe,Masaki Hashimoto,Tomoko Yonezawa,Yuka Jinno,Yasushi Okamura
    • Organizer
      60th International Conference on the Bioscience of Lipids(ICBL2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Voltage sensing phosphatase: crossroad of electrical signal and lipid signal2019

    • Author(s)
      Yasushi Okamura,Tkakahumi Kawai,Akira Kawanabe
    • Organizer
      60th International Conference on the Bioscience of Lipids (ICBL2019), 2nd International Conference onLipoquality Abstracts
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional role of VSP in mouse sperm through its regulation of phosphoinositide distribution2019

    • Author(s)
      Yasushi Okamura
    • Organizer
      The 7th International Ion Channel Conference (IICC-7)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanisms and physiological functions of voltage-sensing phosphatase, VSP2019

    • Author(s)
      Yasushi Okamura
    • Organizer
      Seminar on Vollum Institute, Oregon Health & Science University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Zn2+ sensitivity of Hv1 channel: an evolutionary perspective2019

    • Author(s)
      Adisorn Ratanayotha, Takafumi Kawai, Yasushi Okamura
    • Organizer
      The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cytoplasmic conformational changes of VSP detected by voltage clamp fluorescence spectroscopy2019

    • Author(s)
      Akira Kawanabe, Tomoko Yonezawa, Yasushi Okamura
    • Organizer
      The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The comparison of sensitivity between NaPi-IIa and NaPi-IIb activity to phosphoinositides2019

    • Author(s)
      Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
    • Organizer
      The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hv1/VSOP voltage-gated proton channel inhibits migration in response to fMLF in neutrophils2019

    • Author(s)
      Yoshifumi Okochi, Yasushi Okamura
    • Organizer
      The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A new mechanism of Ca2+-independent voltage-dependent secretion in dorsal root ganglion neurons2019

    • Author(s)
      Zhuan Zhou, Yuan Wang, Hiroki Arima, Rong Huang, Yuqi Hang, Xingyu Du, Feipeng Zhu, Zuying Chai, Changhe Wang, Yasushi Okamura
    • Organizer
      The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional analysis of voltage-sensing phosphatase in mouse sperm2019

    • Author(s)
      Takafumi Kawai, Haruhiko Miyata, Hiroki Nakanishi, Souhei Sakata, Yoshifumi Okochi, Masahiko Watanabe, Kenji Sakimura, Takehiko Sasaki, Masahito Ikawa, Yasushi Okamura
    • Organizer
      The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Electrophysiological characterization of two types of Na-Pi cotransporters2018

    • Author(s)
      Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
    • Organizer
      The 49th NIPS International Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of two-step cytoplasmic conformational changes of voltage sensing phosphatase2018

    • Author(s)
      Akira Kawanabe, Masaki Hashimoto, Tomoko Yonezawa, Yuka Jinno, Yasushi Okamura
    • Organizer
      The 49th NIPS International Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Toward understanding of membrane potential in phagosomai membrane2018

    • Author(s)
      Yoshifumi Okochi, Hidekazu Tsutsui, Yasushi Okamura
    • Organizer
      The 49th NIPS International Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rapid gating of sea squirt Nav1 channel does not require auxiliary subunit2018

    • Author(s)
      Takafumi Kawai, Natsuki Eguchi, Masaki Hashimoto, Yuki Yabuuchi, Yuka Jinno, Akira Kawanabe, Junko Nishino, Atsuo Nishino, Yasushi Okamura
    • Organizer
      The 49th NIPS International Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metal binding to the voltage-gated proton channel Hv1/VSOP2018

    • Author(s)
      Masayo Iwaki, Kohei Takeshita, Hiroko X. Kondo, Kengo Kinoshita, Yasushi Okamura, Yu Takano, Atsushi Nakagawa, Hideki Kandori
    • Organizer
      The 49th NIPS International Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電位依存性プロトンチャネルHv1/VSOPは、活性酸素の産生量の制御を介し て、好中球の走化性を抑制する2018

    • Author(s)
      大河内善史、岡村康司
    • Organizer
      第111回 近畿生理学談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電位依存性プロトンチャネルは肝臓の糖新生を調節する2018

    • Author(s)
      香山建斗、河合喬文、辰巳翔基、山本浩靖、崎村建司、木原進士、岡村康司
    • Organizer
      第111回 近畿生理学談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Na-Pi cotransporter活性のPI(4,5)P2に対する感受性の解析2018

    • Author(s)
      水谷夏希、大河内善史、岡村康司
    • Organizer
      第111回 近畿生理学談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脊椎動物の稠密な脳の起源をホヤのNav チャネルから理解する2018

    • Author(s)
      江口 夏生、神野 有香、橋本 真宜、河合 喬文、西野 純子、大塚 幸雄、川鍋 陽、西野 敦雄、笹倉 靖徳、岡村 康司
    • Organizer
      第4回ホヤ研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電位依存性ホスファターゼVSPの疎水性膜界面の役割2018

    • Author(s)
      川鍋 陽, 橋本 真宜, 米澤 智子, 神野 有香, 岡村 康司
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The analysis of sensitivity of Na-Pi cotransporter activity to phosphoinositides2018

    • Author(s)
      Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
    • Organizer
      Europhysiology 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Age-dependent regulatory function of microglial voltage-gated proton channels2018

    • Author(s)
      Takafumi Kawai, Keizo Takao, Kenji Sakimura, Tsuyoshi Miyakawa, Yasushi Okamura
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Sea squirt voltage-gated sodium channel : kinship with the origin of vertebrate neural sodium channels2018

    • Author(s)
      Natsuki Eguchi, Masaki Hashimoto, Yuka Jinno, Akira Kawanabe, Takafumi Kawai, Yasushi Okamura
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 膜の疎水領域でのリポクオリティ認識機構とナノ膜ドメインの解明2018

    • Author(s)
      岡村 康司、中川 敦史
    • Organizer
      新学術領域研究「脂質クオリティが解き明かす生命現象」第4 回領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電位依存性脂質ホスファターゼにおける疎水性膜界面の役割2018

    • Author(s)
      川鍋陽、橋本真宜、米澤智子、神野有香、岡村康司
    • Organizer
      新学術領域研究「脂質クオリティが解き明かす生命現象」第4 回領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] イノシトールリン脂質によるナトリウム依存性リン酸トランスポーターの活性制御2018

    • Author(s)
      水谷夏希、大河内善史、岡村康司
    • Organizer
      新学術領域研究「脂質クオリティが解き明かす生命現象」第4 回領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] Mechanisms and physiological roles of voltage-sensing phosphatase2018

    • Author(s)
      Yasushi Okamura, Akira Kawanabe, Kazuhisa Nishizawa, Takafumi Kawai
    • Organizer
      The 49th NIPS International Symposium
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 電位依存性ホスファターゼVSP:14年を経て見えたこと、見えないこと2018

    • Author(s)
      岡村 康司、河合 喬文、川鍋 陽、筒井 秀和、坂田 宗平、佐々木 雄彦
    • Organizer
      心血管膜輸送研究会2018「心臓・血管系の頑健性と精緻な制御を支える分子基盤の統合的解明」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 電位依存性ホスファターゼにおける膜電位変化から脱リン酸化反応の変換機構2018

    • Author(s)
      岡村 康司, 川鍋 陽, 坂田 宗平, 神野 有香
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Coupling from electric signal to lipid signal; voltage-sensing phosphoinositide phosphatase2018

    • Author(s)
      Yasushi Okamura
    • Organizer
      Institut NeuroMyoGene, LES SEMINAIRES DE L’INMG
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Ion-permeable voltage sensor domains2018

    • Author(s)
      Yasushi Okamura
    • Organizer
      WCP2018 KYOTO Satellite Symposia New Insights into Ion Channel Functions and Pharmacology
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 膜電位感受性イノシトールリン脂質ホスファターゼの動作原理とその応用2018

    • Author(s)
      岡村 康司
    • Organizer
      2018年 生理学研究所研究会 オルガネラ膜ナノドメインの機能と動態
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 電位依存性ホスファターゼVSPの膜相互作用部位による酵素活性調節2018

    • Author(s)
      川鍋陽、橋本真宜、米澤智子、神野有香、岡村康司
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Na-Pi transporterの活性制御機構2018

    • Author(s)
      水谷夏希、大河内善史、岡村康司
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 貪食細胞における食胞膜電位変化の計測2018

    • Author(s)
      大河内善史、筒井秀和、岡村康司
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 実験と数理モデルに基づいたTRPM4チャネル催不整脈性の多階層解析2018

    • Author(s)
      胡耀鵬、沈揚華、倉原琳、平石敬三、市川純、沼田朋大、岡村康司、朱欣、井上隆司
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 精子における電位依存性ホスファターゼによるイノシトールリン脂質の制御2018

    • Author(s)
      河合 喬文、宮田 治彦、中西 広樹、坂田 宗平、大河内 善史、渡辺 雅彦、﨑村 建司、佐々木 雄彦、伊川 正人、岡村 康司
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Voltage-sensor domain proteins; from structural mechanisms to voltage reporter2018

    • Author(s)
      Yasushi Okamura
    • Organizer
      KI-OU Joint Symposium in Neuroscience
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 電位依存性ホスファターゼの機能解析2017

    • Author(s)
      川鍋陽, 坂田宗平, 筒井秀和, 岡村康司, 近藤寛子,米澤康滋, 鷹野優, 成田宏隆, 中川敦史
    • Organizer
      CREST平成29年度領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウス精子における電位依存性ホスファターゼの機能2017

    • Author(s)
      河合喬文、宮田治彦、中西広樹、坂田宗平、大河内善史、渡辺雅彦、崎村建司、佐々木雄彦、伊川正人、岡村康司
    • Organizer
      第110回 近畿生理学談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電位依存性ホスファターゼVSP の膜近傍領域の役割2017

    • Author(s)
      川鍋陽、橋本真宜、米澤智子、神野有香、坂田宗平、岡村康司
    • Organizer
      第110回 近畿生理学談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電位依存性プロトンチャネルが肝臓の糖代謝に寄与する可能性について2017

    • Author(s)
      香山建斗、河合喬文、辰巳翔基、山本浩靖、崎村建司、木原進、岡村康司
    • Organizer
      第110回 近畿生理学談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genetically encoded fluorescent amino acid for structure function studies of ion channels2017

    • Author(s)
      Claudio Tiecher, Akira Kawanabe, Yasushi Okamura, Armagan Kocer
    • Organizer
      DutchBioPhysics conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of histidine and carboxylate residues for zinc inhibition in the voltage-gated proton channel Hv1/VSOP2017

    • Author(s)
      Masayo Iwaki, Kohei Takeshita, Hiroki Arima, Yasushi Okamura, Atsushi Nakagawa, Hideki Kandori
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The critical role of the hydrophobic membrane interacting region in voltage-sensing phosphatase (VSP)2017

    • Author(s)
      Akira Kawanabe, Masaki Hashimoto, Tomoko Yonezawa, Yuka Jinno, Souhei Sakata, Yasushi Okamura
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電位依存性ホスファターゼVSPの酵素活性に対する膜近傍領域の役割2017

    • Author(s)
      川鍋陽、橋本真宜、米澤智子、神野有香、坂田宗平、岡村康司
    • Organizer
      平成29年度生理学研究所研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Gene expression and behavior analysis of microglial voltage-gated proton channels in mouse2017

    • Author(s)
      Takafumi Kawai, Keizo Takao, Kenji Sakimura, Tsuyoshi Miyakawa, Yasushi Okamura
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of a chemiluminescent voltage indicator applicable to brain activity recording in freely moving multiple mice2017

    • Author(s)
      Shigenori Inagaki, Masakazu Agetsuma, Hidekazu Tsutsui, Shinya Ohara, Yoshiyuki Arai, Kazushi Suzuki, Yuka Jinno, Tomoki Matsuda, Toshio Iijima, Yasushi Okamura, Takeharu Nagai
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 膜の疎水領域でのリポクオリティ認識機構とナノ膜ドメインの解明2017

    • Author(s)
      岡村康司
    • Organizer
      新学術領域研究「脂質クオリティが解き明かす生命現象」第3回領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] In vivo site specific labeling of the voltage-gated potassium channel Kv4.3 for structure function studies: prospects on understanding the neurodegenerative disorder spinocerebellar ataxia 19/22 at the molecular level2017

    • Author(s)
      Claudio Tiecher, Akira Kawanabe, Yasushi Okamura, Armagan Kocer
    • Organizer
      Dutch Neuroscience Meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] がん抑制タンパク質PTENのPIP結合の際のN末端の役割2017

    • Author(s)
      神田直樹, 成田宏隆, 岡村康司, 中川敦史
    • Organizer
      第16回日本蛋白質科学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Coupling Mechanisms of Voltage-Sensing Phosphatase2017

    • Author(s)
      Yasushi Okamura
    • Organizer
      Emerging Concepts in Ion Channel Biophysics
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Atypical cation conductance through voltage sensor domain2017

    • Author(s)
      Yasushi Okamura, Hiroki Arima, Hidekazu Tsutsui
    • Organizer
      Seminar on Institute of Biotechnology National Autonomous University of Mexico
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Molecular dynamics study of kinetics of the voltage-gated proton channel VSOP/Hv1 under electric fields2017

    • Author(s)
      Hiroko X. Kondo, Yasushige Yonezawa, Naoyuki Miyashita, Masayo Iwaki, Kohei Takeshita, Yuichiro Fujiwara, Matsuyuki Shirota, Kengo Kinoshita, Yasushi Okamura, Atsushi Nakagawa, Hideki Kandori, Yu Takano
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Structural analysis of Voltage-sensing phosphatase (VSP) on the electrochemical coupling between the Voltage sensor domain and cytoplasmic phosphatase.2017

    • Author(s)
      Hirotaka Narita, Naoki Kanda, Yasushi Okamura, Atsushi Nakagawa
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Structural Insight of Zinc Binding of Hv1/VSOP in Resting State2017

    • Author(s)
      Atsushi Nakagawa, Masayo Iwaki, Hideki Kandori, Hiroko Kondo, Yasushige Yonezawa, Yu Takano, Hiroki Arima, Yuichiro Fujiwara, Kohei Takeshita, Yasushi Okamura
    • Organizer
      24th Congress & General Assembly of the International Union of Crystallography 2017 (IUCr2017)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Structural Studies of Voltage Sensing-Phosphatase2017

    • Author(s)
      Atsushi Nakagawa, Hirotaka Narita, Naoki Kanda, Akira Kawanabe, Yasushi Okamura
    • Organizer
      The 2nd Molecular and Cellular Life Sciences (MCLS2017)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Roles of membrane interaction in electrochemical coupling in voltage-sensing phosphatase2017

    • Author(s)
      Yasushi Okamura, Akira Kawanabe, Kazuhisa Nishizawa, Souhei Sakata
    • Organizer
      Cold Spring Harbor Conferences Asia
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Principle and biodiversity of voltage sensor domain in channels and enzyme2017

    • Author(s)
      Yasushi Okamura
    • Organizer
      Campus Asia faculty meeting
    • Place of Presentation
      大阪府吹田市
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 電位依存性ホスファターゼにおける膜相互作用の動態と役割2017

    • Author(s)
      岡村 康司、川鍋 陽、西澤 和久、中川 敦史、成田 宏隆、坂田 宗平、神野 有香
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      静岡県浜松市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 初代培養ミクログリアにおける電位依存性プロトンチャネルのダイナミクス2017

    • Author(s)
      河合 喬文、川村 名子、小泉 修一、阿部 学、崎村 建司、岡村 康司
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      静岡県浜松市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Voltage-gated proton channel in zebrafish (DrHv1): from culture dish to living animal2017

    • Author(s)
      Adisorn Ratanayotha, Takafumi Kawai, Yasushi Okamura
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      静岡県浜松市
    • Related Report
      2016 Annual Research Report
  • [Presentation] カルシウムイオン透過性を持つ電位センサードメインの機能解析2017

    • Author(s)
      有馬 大貴、筒井 秀和、岡村 康司
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      静岡県浜松市
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス好中球においてHv1/VSOPはfMLPに対する走化性行動を抑制する2017

    • Author(s)
      大河内 善史、岡村 康司
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      静岡県浜松市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 電位依存性ホスファターゼVSPの新規膜相互作用部位の重要性2017

    • Author(s)
      川鍋 陽、橋本 真宜、米澤 智子、神野 有香、坂田 宗平、岡村 康司
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      静岡県浜松市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 電位依存性ホスファターゼの構造生物学的研究2017

    • Author(s)
      中川 敦史、成田 宏隆、神田 直樹、川鍋 陽、岡村 康司
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      静岡県浜松市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of voltage-gated proton channel in zebrafish2016

    • Author(s)
      Adisorn Ratanayota, Takafumi Kawai, Yasushi Okamura
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] CatSperの電位センサードメインはカルシウム透過性を持つ2016

    • Author(s)
      有馬大貴、筒井秀和、竹下浩平、中川敦史、坂本恵香、吉田学、岡村康司
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 非天然アミノ酸Anapで検出された電位センサーの動き2016

    • Author(s)
      筒井秀和、神野有香、坂田宗平、岡村康司
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膜脂質と相互作用する可能性のある相同領域による電位依存性ホスファターゼVSPとPTENの機能制御2016

    • Author(s)
      川鍋陽、神野有香、坂田宗平、岡村康司
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 好中球の走化性における電位依存性プロトンチャネルの役割2016

    • Author(s)
      大河内善史、岡村康司
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウス精子における電位依存性ホスファターゼの機能2016

    • Author(s)
      河合喬文、宮田治彦、中西広樹、坂田宗平、有馬大貴、宮脇奈那、大河内善史、渡辺雅彦、崎村建司、佐々木雄彦、伊川正人、岡村康司
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] CatSper has a calcium-permeable voltage sensor domain.2016

    • Author(s)
      Hiroki Arima, Hidekazu Tsutsui, Kohei Takeshita, Atsushi Nakagawa, Ayako Sakamoto, Manabu Yoshida and Yasushi Okamura
    • Organizer
      The Biophysical Society 60th Annual Meeting
    • Place of Presentation
      Los Angeles, USA
    • Year and Date
      2016-02-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Principle and biodiversity of voltage sensor domain proteins2016

    • Author(s)
      Yasushi Okamura
    • Organizer
      Dutch Neuroscience Meeting
    • Place of Presentation
      Lunteren, Netherlands
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] How does voltage sensor domain regulate downstream effecter?: lesson from voltage-sensor domain proteins2016

    • Author(s)
      Yasushi Okamura, Souhei Sakata, Akira Kawanabe, Yuichiro Fujiwara, Yuka Jinno
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Molecular mechanisms of voltage-sensing phosphatase, VSP2016

    • Author(s)
      Yasushi Okamura
    • Organizer
      2016 International Conference of Physiological Sciences
    • Place of Presentation
      中国・北京
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Crossroad of electricity and lipid signaling: voltage-sensing phosphoinositide phosphatase (VSP)2016

    • Author(s)
      Yasushi Okamura, Akira Kawanabe, Souhei Sakata
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 電位依存性ホスファターゼVSPとPTENに共通する膜との相互作用部位の重要性2016

    • Author(s)
      川鍋陽、米澤智子、岡村康司
    • Organizer
      第63回日本生化学会 近畿支部例会
    • Place of Presentation
      兵庫県神戸市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 膜の疎水領域でのリポクオリティ認識機構とナノなくドメインの解明2016

    • Author(s)
      岡村康司
    • Organizer
      新学術領域研究「脂質クオオリティが解き明かす生命現象」第2回領域会議
    • Place of Presentation
      静岡県伊豆市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Bidirectional regulation of ROS production by voltage-gated proton channels in microglia2016

    • Author(s)
      Takafumi Kawai,Yoshifumi Okochi,Tomohiko Ozaki,Yoshio Imura,Kenji Sakimura,Schuichi Koizumi,Toshihide Yamashita,Yasushi Okamura
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2016 Annual Research Report
  • [Presentation] The common regulation of phosphatase activity of voltage-sensing phostophase (VSP) and PTEN by conserved putative membrane interacting region2016

    • Author(s)
      Akira Kawanabe, Tomoko Yonezawa, Yuka Jinno, Souhei Sakata, Yasushi Okamura
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城県仙台市
    • Related Report
      2016 Annual Research Report
  • [Presentation] CatSperの電位センサードメインはカルシウムイオン透過性を持つ2016

    • Author(s)
      有馬大貴、筒井秀和、吉田学、岡村康司
    • Organizer
      第3回ホヤ研究会
    • Place of Presentation
      大阪府豊中市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of Voltage-Gated Proton Channel in Zebrafish. (DrHv1)2016

    • Author(s)
      Adisorn Ratanayotha,Takafumi Kawai, Yasushi Okamura
    • Organizer
      第109回近畿生理学談話会
    • Place of Presentation
      大阪府大阪市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 電位依存性プロトンチャネルノックアウトマウスの代謝表現型解析2016

    • Author(s)
      辰巳翔基、河合喬文、山本浩靖、崎村建司、木原進士、岡村康司
    • Organizer
      第109回近畿生理学談話会
    • Place of Presentation
      大阪府大阪市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞内膜の膜電位を可視化する-貪食細胞の食胞膜電位の測定-2016

    • Author(s)
      大河内善史、岡村康司
    • Organizer
      第109回近畿生理学談話会
    • Place of Presentation
      大阪府大阪市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Direct observation og voltage-gated proton channels by high speed AFM2016

    • Author(s)
      Hayato Yamashita, Akira Kawanabe, Yasushi Okamura, Msayuki Abe
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of a chemiluminescent voltage indicator applicable to brain activity recording in freely moving mice2016

    • Author(s)
      Shigenori Inagaki, Masakazu Agetsuma, Hidekazu Tsutsui, Yoshiyuki Arai, Kazushi Suzuki, Yuka Jinno, Yasushi Okamura, Tomoki Matsuda, Takeharu Nagai
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2016 Annual Research Report
  • [Presentation] The role of membrane interacting region of phosphatase domain in voltage-sensing phosohatase (VSP)2016

    • Author(s)
      Akira Kawanabe, Masaki Hashimoto, Tomoko Yonezawa, Yuka Jinno, Souhei Sakata, Yasushi Okamura
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Metal binding to the voltage-gated proton channel VSOP studied by ATR-FTIR2016

    • Author(s)
      Masayo Iwaki, Kohei takeshita, Yasushi Okamura, Atushi Nakagawa, Hideki Kandori
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 膜電位非印加状態で開状態をとるVSOP/Hv1変異体から見る2量体間協調性2016

    • Author(s)
      木村仁美、持田理子、渋村里美、中川敦史、岡村康司、竹下浩平
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規電位センサータンパク質VSOP2のX線結晶構造解析に向けて2016

    • Author(s)
      西川翔士、成田宏隆、岡村康司、中川敦史
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 自由行動マウスの脳活動計測を可能にする化学発光膜電位センサーの開発2016

    • Author(s)
      稲垣成矩、揚妻正和、筒井秀和、新井由之、鈴木和志、神野有香、岡村康司、松田知己、永井健治
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2016 Annual Research Report
  • [Presentation] How is VSP’s enzyme activity activated by intrinsic voltage sensor?2015

    • Author(s)
      Yasushi Okamura
    • Organizer
      RECI V 5th Spanish Ion Channel Network Meeting
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2015-10-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] CatSper has a calcium-permeable voltage sensor domain.2015

    • Author(s)
      Hiroki Arima, Hidekazu Tsutsui and Yasushi Okamura
    • Organizer
      RECI V 5th Spanish Ion Channel Network Meeting
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2015-10-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 複数の光操作と組み合わせた膜電位メージングと薬剤スクリーニングの可能性を広げる発光指示薬の開発2015

    • Author(s)
      稲垣成矩,松田知己,新井由之,神野有香,筒井秀和,岡村康司,永井健治
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      石川県金沢市
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 全反射赤外分光で見る電位依存性プロトンチャネルVSOPへの金属結合2015

    • Author(s)
      岩城雅代, 竹下浩平, 岡村康司, 中川敦史, 神取秀樹
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      石川県金沢市
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] カルシウムイオン透過性を持つ電位センサードメインの機能解析2015

    • Author(s)
      有馬大貴、筒井秀和、岡村康司
    • Organizer
      生理研研究会”膜システムの機能的・構造的統合”
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular mechanisms of voltage sensing phosphatase, VSP2015

    • Author(s)
      Yasushi Okamura
    • Organizer
      "岡崎統合バイオサイエンスセンター サマースクール2015 Development of Biosensing Research"
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2015-08-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] "電位依存性プロトンチャネルによるミクログリア活性酸素産生の制御2015

    • Author(s)
      河合 喬文、大河内 善史、井村 誉史雄、崎村 建司、小泉 修一、岡村 康司
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Determinants of activation rate of voltage-gated proton channel revealed by chimeric constructs between two orthologs2015

    • Author(s)
      Yasushi Okamura
    • Organizer
      TMMU Neuroscience Symposium : Ion Channels
    • Place of Presentation
      中華人民共和国、重慶
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 最小イオンチャネルHv1/VSOPによる貪食細胞の活性酸素産生の多重制御2015

    • Author(s)
      岡村康司
    • Organizer
      日本バイオイメージング学会シンポジウム
    • Place of Presentation
      東京都葛飾区
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 電位依存性プロトンチャネルの2量体と単量体間のゲーティング特性の違いを増大させる変異体の解析2015

    • Author(s)
      川鍋陽、岡村康司
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      石川県金沢市
    • Related Report
      2015 Annual Research Report
  • [Presentation] カルシウムイオン透過性を持つ電位センサートドメインの機能解析2015

    • Author(s)
      有馬大貴、筒井秀和、岡村康司
    • Organizer
      第108回近畿生理談話会
    • Place of Presentation
      大阪府東大阪市
    • Related Report
      2015 Annual Research Report
  • [Presentation] 電位依存性ホスファターゼVSPの構造基盤の解明2015

    • Author(s)
      岡村康司、川鍋陽、坂田宗平、筒井秀和、中川敦史、成田宏隆、鷹野優、神取秀樹
    • Organizer
      ライフサイエンスの革新を目指した構造生命科学と先端的基盤技術
    • Place of Presentation
      東京都文京区
    • Related Report
      2015 Annual Research Report
  • [Book] 脂質クオリティ2018

    • Author(s)
      岡村康司、大澤匡範
    • Total Pages
      220
    • Publisher
      羊土社
    • ISBN
      9784758103718
    • Related Report
      2018 Annual Research Report
  • [Book] ガイトン生理学 原著第13版2018

    • Author(s)
      石川義弘、岡村康司(総監訳)、尾仲達史、河野憲二
    • Total Pages
      1100
    • Publisher
      エルゼビア・ジャパン株式会社
    • ISBN
      9784860347741
    • Related Report
      2017 Annual Research Report
  • [Book] ギャノング生理学 原書25版2017

    • Author(s)
      岡田泰伸、佐久間 康夫、岡村 康司 (監訳)
    • Total Pages
      920
    • Publisher
      丸善出版
    • ISBN
      9784621301883
    • Related Report
      2017 Annual Research Report
  • [Book] Voltage-gated proton channels2017

    • Author(s)
      Okamura Y.
    • Publisher
      Reference Module in Life Sciences, Elsevier
    • ISBN
      9780128096338
    • Related Report
      2017 Annual Research Report
  • [Book] Reference Module in Life Sciences, 'Voltage-gated proton channels'2017

    • Author(s)
      Y. Okamura
    • Publisher
      Elsevier
    • Related Report
      2016 Annual Research Report

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Published: 2015-07-02   Modified: 2021-01-27  

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