膜の疎水領域でのリポクオリティ認識機構とナノ膜ドメインの解明

• Project Area: Quality of lipids in biological systems
• Project/Area Number: 15H05901
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: 岡村 康司 大阪大学, 医学系研究科, 教授 (80201987)
• Co-Investigator(Kenkyū-buntansha): 中川 敦史 大阪大学, 蛋白質研究所, 教授 (20188890)
• Project Period (FY): 2015-06-29 – 2020-03-31
• Project Status: Granted (Fiscal Year 2019)
• Budget Amount: ¥95,290,000 (Direct Cost: ¥73,300,000、Indirect Cost: ¥21,990,000); Fiscal Year 2019: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2018: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2017: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2016: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2015: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
• Keywords: 脂質 / 蛋白質 / シグナル伝達 / 酵素 / 生理学 / イオンチャネル / タンパク質ドメイン / 電位センサー / イノシトールリン脂質

Outline of Annual Research Achievements

哺乳類培養細胞でのイノシトールリン脂質の動態を解析する効果的な分子ツールとしてこれまでに世界的に用いられてきた脊椎動物ゼブラフィッシュ由来Voltage-sensing phosphatase（Dr-VSP）について、前年度までにホヤ由来VSP（Ci-VSP）での詳細な解析から見出したホスファターゼドメイン中のhydrophobic spineの膜脂質との相互作用候補アミノ酸部位を改変することにより、電位依存性酵素活性を向上させることによる分子ツールの改良が可能かを、主に発現系細胞HEK293T細胞での電気生理学的解析、蛍光顕微鏡をもちいた発現解析によって検討した。
これまでの脂質のリポクオリティを認識する分子機序としてCi-VSPのhydrophobic spineのL284に対応するL223をFに変異させた変異体は電位依存的酵素活性を1.7倍増加させた（Kir2.1活性での評価）。更に細胞膜への限局を向上させるため、これまでの解析から細胞膜へのターゲットを向上させることが知られていたCi-VSPのN末端側細胞内領域とのキメラを作成した（CiDr-VSP-mChe-L223F）（表面での限局を確認するため蛍光タンパク質も融合させた）。これにより野生型のDr-VSPよりも2.1倍から2.8倍に電位依存的酵素活性を向上させることに成功した。また、研究分担者の中川のチームによる解析によって、様々なイノシトールリン脂質を基質としたVSPの共結晶を作製し高分解能での構造情報を得ることに成功した。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

これまでに明らかにしてきたVSPの膜との相互作用部位hydrophobic spineが、ホヤ由来VSPだけでなく脊椎動物のVSPについても保存されていることが明らかになり、さらにこの部位のアミノ酸変異により、イノシトールリン脂質のレベルを短時間で変化させる分子ツールとしての性質を向上させることに成功した。VSPのX線結晶解析についても、基質との共結晶が複数得られており、結合部位の構造基盤が明らかになりつつある。

Strategy for Future Research Activity

VSPについてはリポクオリティ認識機構の原理を深く知るため、Ci-VSPについて、これまでに確立してきた発現系細胞での非天然アミノ酸ANAPを用いたVoltage clamp fluorometry法を更に工夫し、側鎖間での相互作用を解析する。これまでに電位依存的なスペクトルの変化の計測が可能であったH182Anapのコンストラクトを用いて、更に解像度の高いシグナルを得られるかを検討する。またこのシグナルの分子機構について、他の部位に2つ目の変異を導入するなどによる解析をおこなって明らかにする。VSPの構造については、これまでに得てきた様々な基質との共結晶構造について更に分解能を上げられるかを検討する（研究分担者中川）。

Research Products

• [Journal Article] The Slo3/Lrrc52 complex is sensitive to phosphoinotsitides. (2020)
  • Author(s): Kawai T, Okamura Y.
  • Journal Title: Channels Volume: -
• [Journal Article] Hv1/VSOP regulates neutrophil directional migration and ERK activity by tuning ROS production. (2020)
  • Author(s): Okochi Y, Umemoto E, Okamura Y.
  • Journal Title: J Leukoc Biol . Volume: 107 Pages: 819-831
  • DOI: 10.1002/jlb.2a0320-110rr
  • Peer Reviewed
• [Journal Article] Activation Mechanisms of Voltage Sensing Phosphatase (2020)
  • Author(s): 川鍋 陽. 西澤和久. 岡村康司
  • Journal Title: Biophysics Volume: 60 Issue: 2 Pages: 105-107
  • DOI: 10.2142/biophys.60.105
  • NAID: 130007818361
  • ISSN: 0582-4052, 1347-4219
• [Journal Article] Engineering an enhanced voltage-sensing phosphatase (2020)
  • Author(s): Kawanabe Akira、Mizutani Natsuki、Polat Onur K.、Yonezawa Tomoko、Kawai Takafumi、Mori Masayuki X.、Okamura Yasushi
  • Journal Title: Journal of General Physiology Volume: 152
  • DOI: 10.1085/jgp.201912491
  • Peer Reviewed
• [Journal Article] Polarized PtdIns(4,5)P2distribution mediated by a voltage-sensing phosphatase (VSP) regulates sperm motility (2019)
  • Author(s): Kawai Takafumi、Miyata Haruhiko、Nakanishi Hiroki、Sakata Souhei、Morioka Shin、Sasaki Junko、Watanabe Masahiko、Sakimura Kenji、Fujimoto Toyoshi、Sasaki Takehiko、Ikawa Masahito、Okamura Yasushi
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 116 Pages: 26020-26026
  • DOI: 10.1073/pnas.1916867116
  • Peer Reviewed / Open Access
• [Journal Article] Functional analysis of a double-point mutation in the KCNJ2 gene identified in a family with Andersen-Tawil syndrome (2019)
  • Author(s): Fukumura Shinobu、Yamauchi Kosuke、Kawanabe Akira、Yamamoto Akiyo、Nakaza Maki、Kubota Tomoya、Kato Shinsuke、Sasaki Ryogen、Okamura Yasushi、Takahashi Masanori P.
  • Journal Title: Journal of the Neurological Sciences. Volume: 407 Pages: 116521-116521
  • DOI: 10.1016/j.jns.2019.116521
  • Peer Reviewed
• [Journal Article] Distinct functional properties of two electrogenic isoforms of the SLC 34 Na‐Pi cotransporter (2019)
  • Author(s): Mizutani Natsuki、Okochi Yoshifumi、Okamura Yasushi
  • Journal Title: Physiol. Reports Volume: 7
  • DOI: 10.14814/phy2.14156
  • Peer Reviewed / Open Access
• [Journal Article] ホスフォイノシチドによるチャネル・トランスポーターの制御 (2019)
  • Author(s): 岡村康司、水谷夏希、大河内善史
  • Journal Title: 医学のあゆみ Volume: 269 Pages: 1108-1113
• [Journal Article] Real-time functional analysis of Hv1 channel in neutrophils: a new approach from zebrafish model (2019)
  • Author(s): Ratanayotha Adisorn、Kawai Takafumi、Okamura Yasushi
  • Journal Title: American Journal of Physiology Volume: 316 Pages: 819-831
  • DOI: 10.1152/ajpregu.00326.2018
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of coupling to voltage sensors in voltage-evoked cellular signals (2019)
  • Author(s): OKAMURA Yasushi、OKOCHI Yoshifumi
  • Journal Title: Proc. Jpn. Acad., Ser. B Volume: 95 Issue: 3 Pages: 111-135
  • DOI: 10.2183/pjab.95.010
  • NAID: 130007610041
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dynamic structural rearrangements and functional regulation of voltage‐sensing phosphatase (2019)
  • Author(s): Sakata S and Okamura Y
  • Journal Title: The Journal of Physiology Volume: 597 Pages: 29-40
  • DOI: 10.1113/jp274113
  • Peer Reviewed / Open Access
• [Journal Article] The hydrophobic nature of a novel membrane interface regulates the enzyme activity of a voltage-sensing phosphatase (2018)
  • Author(s): Kawanabe A, Hashimoto M, Mishizawa M, Nishizawa N, Narita H, Yonezawa T, Jinno Y, Sakata S, Nakawaga A and Okamura Y
  • Journal Title: eLife Volume: 7 Pages: 2097-2131
  • DOI: 10.7554/elife.41653
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Zn2+-Binding to the Voltage-Gated Proton Channel Hv1/VSOP (2018)
  • Author(s): Iwaki Masayo、Takeshita Kohei、Kondo Hiroko X.、Kinoshita Kengo、Okamura Yasushi、Takano Yu、Nakagawa Atsushi、Kandori Hideki
  • Journal Title: The Journal of Physical Chemistry B Volume: 122 Pages: 9076-9080
  • DOI: 10.1021/acs.jpcb.8b04890
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tracking the sarcoplasmic reticulum membrane voltage in muscle with a FRET biosensor (2018)
  • Author(s): Sanchez Colline、Berthier Christine、Allard Bruno、Perrot Jimmy、Bouvard Cl?ment、Tsutsui Hidekazu、Okamura Yasushi、Jacquemond Vincent
  • Journal Title: The Journal of General Physiology Volume: 150 Pages: 1163-1177
  • DOI: 10.1085/jgp.201812035
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Conservation of the Ca2+-permeability through the voltage sensor domain of mammalian CatSper subunit (2018)
  • Author(s): Arima Hiroki、Tsutsui Hidekazu、Okamura Yasushi
  • Journal Title: Channels Volume: 12 Pages: 240-248
  • DOI: 10.1080/19336950.2018.1476791
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Induction of divalent cation permeability by heterologous expression of a voltage sensor domain (2018)
  • Author(s): Arima Hiroki、Tsutsui Hidekazu、Sakamoto Ayako、Yoshida Manabu、Okamura Yasushi
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Biomembranes Volume: 1860 Pages: 981-990
  • DOI: 10.1016/j.bbamem.2018.01.004
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mechanistic insight into the suppression of microglial ROS production by voltage-gated proton channels(VSOP/Hv1) (2018)
  • Author(s): Kawai T, Tatsumi S, Kihara S, Sakimura K, Okamura Y.
  • Journal Title: Channels Volume: 12 Issue: 1 Pages: 43108-43108
  • DOI: 10.1080/19336950.2017.1385684
  • Peer Reviewed / Open Access
• [Journal Article] Voltage-Sensing Phosphatases: Biophysics, Physiology, and Molecular Engineering (2018)
  • Author(s): Okamura Yasushi、Kawanabe Akira、Kawai Takafumi
  • Journal Title: Physiological Reviews Volume: 98 Pages: 2097-2131
  • DOI: 10.1152/physrev.00056.2017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evolutionary History of Voltage-Gated Sodium Channels. (2018)
  • Author(s): Nishino Atsuo, Okamura Yasushi
  • Journal Title: Handbook of Experimental Pharmacology, Springer Volume: Epub ahead of print Pages: 1-30
  • DOI: 10.1007/164_2017_70
  • Peer Reviewed
• [Journal Article] 脂質－イオンチャネル相互連関 (2018)
  • Author(s): 岡村康司、大澤匡範
  • Journal Title: 「脂質クオリティ」実験医学増刊 Volume: vol.36, No.10 Pages: 97-104
  • Peer Reviewed
• [Journal Article] Unconventional role of voltage-gated proton channels (VSOP/Hv1) in regulation of microglial ROS production. (2017)
  • Author(s): Kawai T, Okochi Y, Ozaki T, Imura Y, Koizumi S, Yamazaki M, Abe M, Sakimura K, Yamashita T, Okamura Y.
  • Journal Title: J Neurochem. Volume: 142 Issue: 5 Pages: 686-699
  • DOI: 10.1111/jnc.14106
  • Peer Reviewed / Open Access
• [Journal Article] Molecular and functional characterization of the voltage-gated proton channel in zebrafish neutrophils (2017)
  • Author(s): Ratanayotha Adisorn、Kawai Takafumi、Higashijima Shin-ichi、Okamura Yasushi
  • Journal Title: Physiol Rep Volume: 5
  • DOI: 10.14814/phy2.13345
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 電位センサードメインを用いた膜電位プローブの進歩 (2017)
  • Author(s): 河合 喬文、筒井 秀和、岡村 康司
  • Journal Title: 生体の科学 Volume: 68 Issue: 5 Pages: 444-445
  • DOI: 10.11477/mf.2425200686
  • ISSN: 0370-9531, 1883-5503
  • Peer Reviewed
• [Journal Article] AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate. (2017)
  • Author(s): Shinobu Hirai, Kohji Hotta, Yoshihiro Kubo, Atsuo Nishino, Shigeo Okabe, Yasushi Okamura, Haruo Okado
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: - Pages: 3939-3944
  • DOI: 10.1073/pnas.1612943114
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. (2017)
  • Author(s): Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda M, and Nagai T
  • Journal Title: Scientific Report Volume: 7 Pages: 42398-42398
  • DOI: 10.1038/srep42398
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comparison between mouse and sea urchin orthologs of voltage-gated proton channel suggests role of S3 segment in activation gating (2016)
  • Author(s): Sakata S, Miyawaki N, McCormack TJ, Arima H, Kawanabe A, Ozkucur N, Kurokawa T, Jinno Y, Fujiwara Y, Okamura Y
  • Journal Title: Biochim. Biophys. Acta Volume: 1858 Pages: 2972-2983
  • DOI: 10.1016/j.bbamem.2016.09.008
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Voltage-dependent motion of the catalytic region of voltage-sensing phosphatase monitored by a fluorescent amino acid (2016)
  • Author(s): Sakata S, Jinno Y, Kawanabe A, Okamura Y
  • Journal Title: Proc Natil Acad Sci USA Volume: 113 Pages: 7521-7526
  • DOI: 10.1073/pnas.1604218113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural basis for the membrane association of ankyrinG via palmitoylation (2016)
  • Author(s): Fujiwara Y, Kondo H, Shirota M, Kobayashi M, Takeshita K, Nakagawa A, Okamura Y, Kinoshita K
  • Journal Title: Scientific Reports Volume: 6 Pages: 23981-23981
  • DOI: 10.1038/srep23981
  • NAID: 120006770875
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recent Views of Proton-Selective Permeation: Lesson from Voltage-Gated Proton Channel (2016)
  • Author(s): 岡村 康司
  • Journal Title: Biophysics Volume: 56 Issue: 3 Pages: 154-158
  • DOI: 10.2142/biophys.56.154
  • NAID: 130005153075
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] Simple scheme of lipid enzyme can explain complex lives of phosphoinositides. (2016)
  • Author(s): Okamura Y.
  • Journal Title: PNAS Volume: 113 Pages: 7012-7014
  • DOI: 10.1073/pnas.1607427113
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SPring-8 BL44XU, beamline designed for structure analysis of large biological macromolecular assemblies (2016)
  • Author(s): Akifumi Higashiura, Eiki Yamashita, Masato Yoshimura, Kazuya Hasegawa, Yukito Furukawa, Takashi, Kumasaka, Go Ueno, Masaki Yamamoto, Tomitake Tsukihara and Atsushi Nakagawa
  • Journal Title: AIP Conference Proceedings Volume: 1741
• [Journal Article] The voltage-gated proton channel Hv1/VSOP inhibits neutrophil granule release (2016)
  • Author(s): Okochi Y, Aratani Y, Adissu HA, Miyawaki N, Sasaki M, Suzuki K, Okamura Y.
  • Journal Title: J Microbiol Methods Volume: 99 Pages: 159-163
  • DOI: 10.1189/jlb.3hi0814-393r
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effects of unsaturated fatty acids on the kinetics of voltage-gated proton channels heterologously expressed in cultured cells. (2016)
  • Author(s): Kawanabe A., Okamura Y.
  • Journal Title: J. Physiol. Volume: 594 Pages: 595-610
  • DOI: 10.1113/jp271274
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Direct Interaction between the Voltage-sensors Produces Cooperative Sustained Deactivation in Voltage-gated H+ Channel Dimers. (2016)
  • Author(s): Okuda H, Yonezawa Y, Takano Y, Okamura Y, Fujiwara Y.
  • Journal Title: J BIOL CHEM Volume: 291 Pages: 5935-47
  • DOI: 10.1074/jbc.m115.666834
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural insight into the regulation mechanism of proton leakage based on crystal structure of voltage-gated proton channel (VSOP) (2015)
  • Author(s): 竹下 浩平，岡村 康司，中川 敦史
  • Journal Title: 生化学 Volume: 87-5 Issue: 5 Pages: 625-628
  • DOI: 10.14952/SEIKAGAKU.2015.870625
  • NAID: 40020650286
  • ISSN: 0037-1017
  • Peer Reviewed
• [Journal Article] Gating mechanisms of voltage-gated proton channels. (2015)
  • Author(s): Okamura Y, Fujiwara Y, Sakata S.
  • Journal Title: Annual Rev. Biochem. Volume: 84 Pages: 685-709
  • DOI: 10.1146/annurev-biochem-060614-034307
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] The conformational change of the cytoplasmic region of voltage-sensing phosphatase (2020)
  • Author(s): Akira Kawanabe,Manami Nishizawa,Kazuhisa Nishizawa,Hirotaka Narita,Tomoko Yonezawa,Yuka Jinno,Souhei Sakata,Atsushi Nakagawa,Yasushi Okamura.
  • Organizer: the 97th Annual Meeting of the Physiological Society of Japan（第97回日本生理学会大会）誌上開催
  • Int'l Joint Research
• [Presentation] Regulation of phosphoinositide distribution by voltage-sensing phosphatase in mouse spermatozoa (2020)
  • Author(s): Takafumi Kawai,Yasushi Okamura
  • Organizer: the 97th Annual Meeting of the Physiological Society of Japan（第97回日本生理学会大会）誌上開催
  • Int'l Joint Research
• [Presentation] Analyzing PI(4,5)P2 sensitivity of GABAA receptor by voltage sensing phosphatase (2020)
  • Author(s): Shunich Sugimoto,Takafumi Kawai,Yasushi Okamura
  • Organizer: the 97th Annual Meeting of the Physiological Society of Japan（第97回日本生理学会大会）誌上開催
  • Int'l Joint Research
• [Presentation] Alteration in the coupling between voltage sensor movement and phosphatase activity in voltage-sensing phosphatase with mutation in voltage sensor domani (2020)
  • Author(s): Natsuki Mizutani, Yasushi Okamura
  • Organizer: the 97th Annual Meeting of the Physiological Society of Japan（第97回日本生理学会大会）誌上開催
  • Int'l Joint Research
• [Presentation] Establishment of a method measuring membrane potential in phagosomes (2020)
  • Author(s): Yoshifumi Okochi,Hidekazu Tsutsui,Yasushi Okamura
  • Organizer: the 97th Annual Meeting of the Physiological Society of Japan（第97回日本生理学会大会）誌上開催
  • Int'l Joint Research
• [Presentation] Coupling Mechanisms of VSD Mutants of CI-VSP (2020)
  • Author(s): Natsuki Mizutani,Yasushi Okamura
  • Organizer: 64th Biophysical Society Annual Meeting
  • Int'l Joint Research
• [Presentation] マウス精子に存在する電位依存性ホスファターゼの機能 (2019)
  • Author(s): 河合喬文、岡村康司
  • Organizer: 第112回近畿生理学談話会
• [Presentation] Regulation of ion channels by distribution of phospholipids in mouse sperm (2019)
  • Author(s): Yasushi Okamura
  • Organizer: Ion Channel Modulation Symposium
  • Int'l Joint Research / Invited
• [Presentation] Improvement of voltage-sensing phosphatase as a molecular tool for phosphoinositide depletion in living cells (2019)
  • Author(s): Akira kawanabe, Natsuki Mizutani, Tomoko Yonezawa, Yasushi Okamura
  • Organizer: The 57th Annual Meeting of the Biophysical Society of Japan
  • Int'l Joint Research
• [Presentation] Evolutional insight into the orign of clustering of voltage-gated sodium channels (2019)
  • Author(s): Yasushi Okamura,Yuka Jinno, Masaki Hashimoto,Junko Nishino,Yasunori Sasakura,Atsuo Nishino,Tamafumi Kawai
  • Organizer: NEURO2019（第42回日本神経科学大会、第62回日本神経化学会大会）
• [Presentation] Analysis of membrane potential of phagosome in phagocytes (2019)
  • Author(s): Yoshifumi Okochi,Hidekazu Tsutsui,Yasushi Okamura
  • Organizer: 60th International Conference on the Bioscience of Lipids（ICBL2019）
  • Int'l Joint Research
• [Presentation] Function of voltage-sensing phosphatase in mouse sperm (2019)
  • Author(s): Takahumi Kawai,Haruhiko Miyata, Hiroko Nakanishi,Souhei Sakata,Yoshifumi Okochi,Masahiko Watanabe,Toyoshi Fujimoto,Kenji Sakimura, Takehiko Sasaki,Masahito Ikawa,Yasushi Okamura
  • Organizer: 60th International Conference on the Bioscience of Lipids（ICBL2019）
  • Int'l Joint Research
• [Presentation] The hydrophobic protein-membrane interface maintains an activity of a phosphoinositide phosphatase (2019)
  • Author(s): Akira Kawanabe,Masaki Hashimoto,Tomoko Yonezawa,Yuka Jinno,Yasushi Okamura
  • Organizer: 60th International Conference on the Bioscience of Lipids（ICBL2019）
  • Int'l Joint Research
• [Presentation] Voltage sensing phosphatase: crossroad of electrical signal and lipid signal (2019)
  • Author(s): Yasushi Okamura,Tkakahumi Kawai,Akira Kawanabe
  • Organizer: 60th International Conference on the Bioscience of Lipids (ICBL2019）, 2nd International Conference onLipoquality Abstracts
  • Int'l Joint Research
• [Presentation] Functional role of VSP in mouse sperm through its regulation of phosphoinositide distribution (2019)
  • Author(s): Yasushi Okamura
  • Organizer: The 7th International Ion Channel Conference (IICC-7)
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms and physiological functions of voltage-sensing phosphatase, VSP (2019)
  • Author(s): Yasushi Okamura
  • Organizer: Seminar on Vollum Institute, Oregon Health ＆ Science University
  • Int'l Joint Research / Invited
• [Presentation] Zn2+ sensitivity of Hv1 channel: an evolutionary perspective (2019)
  • Author(s): Adisorn Ratanayotha, Takafumi Kawai, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research / Invited
• [Presentation] Cytoplasmic conformational changes of VSP detected by voltage clamp fluorescence spectroscopy (2019)
  • Author(s): Akira Kawanabe, Tomoko Yonezawa, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research
• [Presentation] The comparison of sensitivity between NaPi-IIa and NaPi-IIb activity to phosphoinositides (2019)
  • Author(s): Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research
• [Presentation] Hv1/VSOP voltage-gated proton channel inhibits migration in response to fMLF in neutrophils (2019)
  • Author(s): Yoshifumi Okochi, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research / Invited
• [Presentation] A new mechanism of Ca2+-independent voltage-dependent secretion in dorsal root ganglion neurons (2019)
  • Author(s): Zhuan Zhou, Yuan Wang, Hiroki Arima, Rong Huang, Yuqi Hang, Xingyu Du, Feipeng Zhu, Zuying Chai, Changhe Wang, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research / Invited
• [Presentation] Functional analysis of voltage-sensing phosphatase in mouse sperm (2019)
  • Author(s): Takafumi Kawai, Haruhiko Miyata, Hiroki Nakanishi, Souhei Sakata, Yoshifumi Okochi, Masahiko Watanabe, Kenji Sakimura, Takehiko Sasaki, Masahito Ikawa, Yasushi Okamura
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019) , 第96回日本生理学会年会 合同大会
  • Int'l Joint Research / Invited
• [Presentation] Electrophysiological characterization of two types of Na-Pi cotransporters (2018)
  • Author(s): Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] Regulation of two-step cytoplasmic conformational changes of voltage sensing phosphatase (2018)
  • Author(s): Akira Kawanabe, Masaki Hashimoto, Tomoko Yonezawa, Yuka Jinno, Yasushi Okamura
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] Toward understanding of membrane potential in phagosomai membrane (2018)
  • Author(s): Yoshifumi Okochi, Hidekazu Tsutsui, Yasushi Okamura
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] Rapid gating of sea squirt Nav1 channel does not require auxiliary subunit (2018)
  • Author(s): Takafumi Kawai, Natsuki Eguchi, Masaki Hashimoto, Yuki Yabuuchi, Yuka Jinno, Akira Kawanabe, Junko Nishino, Atsuo Nishino, Yasushi Okamura
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] Metal binding to the voltage-gated proton channel Hv1/VSOP (2018)
  • Author(s): Masayo Iwaki, Kohei Takeshita, Hiroko X. Kondo, Kengo Kinoshita, Yasushi Okamura, Yu Takano, Atsushi Nakagawa, Hideki Kandori
  • Organizer: The 49th NIPS International Symposium
  • Int'l Joint Research
• [Presentation] 電位依存性プロトンチャネルHv1/VSOPは、活性酸素の産生量の制御を介し て、好中球の走化性を抑制する (2018)
  • Author(s): 大河内善史、岡村康司
  • Organizer: 第111回 近畿生理学談話会
• [Presentation] 電位依存性プロトンチャネルは肝臓の糖新生を調節する (2018)
  • Author(s): 香山建斗、河合喬文、辰巳翔基、山本浩靖、崎村建司、木原進士、岡村康司
  • Organizer: 第111回 近畿生理学談話会
• [Presentation] Na-Pi cotransporter活性のPI(4,5)P2に対する感受性の解析 (2018)
  • Author(s): 水谷夏希、大河内善史、岡村康司
  • Organizer: 第111回 近畿生理学談話会
• [Presentation] 脊椎動物の稠密な脳の起源をホヤのNav チャネルから理解する (2018)
  • Author(s): 江口 夏生、神野 有香、橋本 真宜、河合 喬文、西野 純子、大塚 幸雄、川鍋 陽、西野 敦雄、笹倉 靖徳、岡村 康司
  • Organizer: 第4回ホヤ研究会
• [Presentation] 電位依存性ホスファターゼVSPの疎水性膜界面の役割 (2018)
  • Author(s): 川鍋 陽, 橋本 真宜, 米澤 智子, 神野 有香, 岡村 康司
  • Organizer: 第91回日本生化学会大会
• [Presentation] The analysis of sensitivity of Na-Pi cotransporter activity to phosphoinositides (2018)
  • Author(s): Natsuki Mizutani, Yoshifumi Okochi, Yasushi Okamura
  • Organizer: Europhysiology 2018
  • Int'l Joint Research
• [Presentation] Age-dependent regulatory function of microglial voltage-gated proton channels (2018)
  • Author(s): Takafumi Kawai, Keizo Takao, Kenji Sakimura, Tsuyoshi Miyakawa, Yasushi Okamura
  • Organizer: 第41回日本神経科学大会
• [Presentation] Sea squirt voltage-gated sodium channel : kinship with the origin of vertebrate neural sodium channels (2018)
  • Author(s): Natsuki Eguchi, Masaki Hashimoto, Yuka Jinno, Akira Kawanabe, Takafumi Kawai, Yasushi Okamura
  • Organizer: 第41回日本神経科学大会
• [Presentation] 膜の疎水領域でのリポクオリティ認識機構とナノ膜ドメインの解明 (2018)
  • Author(s): 岡村 康司、中川 敦史
  • Organizer: 新学術領域研究「脂質クオリティが解き明かす生命現象」第4 回領域会議
• [Presentation] 電位依存性脂質ホスファターゼにおける疎水性膜界面の役割 (2018)
  • Author(s): 川鍋陽、橋本真宜、米澤智子、神野有香、岡村康司
  • Organizer: 新学術領域研究「脂質クオリティが解き明かす生命現象」第4 回領域会議
• [Presentation] イノシトールリン脂質によるナトリウム依存性リン酸トランスポーターの活性制御 (2018)
  • Author(s): 水谷夏希、大河内善史、岡村康司
  • Organizer: 新学術領域研究「脂質クオリティが解き明かす生命現象」第4 回領域会議
• [Presentation] Mechanisms and physiological roles of voltage-sensing phosphatase (2018)
  • Author(s): Yasushi Okamura, Akira Kawanabe, Kazuhisa Nishizawa, Takafumi Kawai
  • Organizer: The 49th NIPS International Symposium
  • Invited
• [Presentation] 電位依存性ホスファターゼVSP：14年を経て見えたこと、見えないこと (2018)
  • Author(s): 岡村 康司、河合 喬文、川鍋 陽、筒井 秀和、坂田 宗平、佐々木 雄彦
  • Organizer: 心血管膜輸送研究会2018「心臓・血管系の頑健性と精緻な制御を支える分子基盤の統合的解明」
  • Invited
• [Presentation] 電位依存性ホスファターゼにおける膜電位変化から脱リン酸化反応の変換機構 (2018)
  • Author(s): 岡村 康司, 川鍋 陽, 坂田 宗平, 神野 有香
  • Organizer: 第91回日本生化学会大会
  • Invited
• [Presentation] Coupling from electric signal to lipid signal; voltage-sensing phosphoinositide phosphatase (2018)
  • Author(s): Yasushi Okamura
  • Organizer: Institut NeuroMyoGene, LES SEMINAIRES DE L’INMG
  • Invited
• [Presentation] Ion-permeable voltage sensor domains (2018)
  • Author(s): Yasushi Okamura
  • Organizer: WCP2018 KYOTO Satellite Symposia New Insights into Ion Channel Functions and Pharmacology
  • Invited
• [Presentation] 膜電位感受性イノシトールリン脂質ホスファターゼの動作原理とその応用 (2018)
  • Author(s): 岡村 康司
  • Organizer: 2018年 生理学研究所研究会 オルガネラ膜ナノドメインの機能と動態
  • Invited
• [Presentation] 電位依存性ホスファターゼVSPの膜相互作用部位による酵素活性調節 (2018)
  • Author(s): 川鍋陽、橋本真宜、米澤智子、神野有香、岡村康司
  • Organizer: 第95回日本生理学会大会
• [Presentation] Na-Pi transporterの活性制御機構 (2018)
  • Author(s): 水谷夏希、大河内善史、岡村康司
  • Organizer: 第95回日本生理学会大会
• [Presentation] 貪食細胞における食胞膜電位変化の計測 (2018)
  • Author(s): 大河内善史、筒井秀和、岡村康司
  • Organizer: 第95回日本生理学会大会
• [Presentation] 実験と数理モデルに基づいたTRPM4チャネル催不整脈性の多階層解析 (2018)
  • Author(s): 胡耀鵬、沈揚華、倉原琳、平石敬三、市川純、沼田朋大、岡村康司、朱欣、井上隆司
  • Organizer: 第95回日本生理学会大会
  • Invited
• [Presentation] 精子における電位依存性ホスファターゼによるイノシトールリン脂質の制御 (2018)
  • Author(s): 河合 喬文、宮田 治彦、中西 広樹、坂田 宗平、大河内 善史、渡辺 雅彦、﨑村 建司、佐々木 雄彦、伊川 正人、岡村 康司
  • Organizer: 第123回日本解剖学会総会・全国学術集会
  • Invited
• [Presentation] Voltage-sensor domain proteins; from structural mechanisms to voltage reporter (2018)
  • Author(s): Yasushi Okamura
  • Organizer: KI-OU Joint Symposium in Neuroscience
  • Invited
• [Presentation] 電位依存性ホスファターゼの機能解析 (2017)
  • Author(s): 川鍋陽, 坂田宗平, 筒井秀和, 岡村康司, 近藤寛子,米澤康滋, 鷹野優, 成田宏隆, 中川敦史
  • Organizer: CREST平成29年度領域会議
• [Presentation] マウス精子における電位依存性ホスファターゼの機能 (2017)
  • Author(s): 河合喬文、宮田治彦、中西広樹、坂田宗平、大河内善史、渡辺雅彦、崎村建司、佐々木雄彦、伊川正人、岡村康司
  • Organizer: 第110回 近畿生理学談話会
• [Presentation] 電位依存性ホスファターゼVSP の膜近傍領域の役割 (2017)
  • Author(s): 川鍋陽、橋本真宜、米澤智子、神野有香、坂田宗平、岡村康司
  • Organizer: 第110回 近畿生理学談話会
• [Presentation] 電位依存性プロトンチャネルが肝臓の糖代謝に寄与する可能性について (2017)
  • Author(s): 香山建斗、河合喬文、辰巳翔基、山本浩靖、崎村建司、木原進、岡村康司
  • Organizer: 第110回 近畿生理学談話会
• [Presentation] Genetically encoded fluorescent amino acid for structure function studies of ion channels (2017)
  • Author(s): Claudio Tiecher, Akira Kawanabe, Yasushi Okamura, Armagan Kocer
  • Organizer: DutchBioPhysics conference 2017
  • Int'l Joint Research
• [Presentation] The role of histidine and carboxylate residues for zinc inhibition in the voltage-gated proton channel Hv1/VSOP (2017)
  • Author(s): Masayo Iwaki, Kohei Takeshita, Hiroki Arima, Yasushi Okamura, Atsushi Nakagawa, Hideki Kandori
  • Organizer: 第55回日本生物物理学会年会
• [Presentation] The critical role of the hydrophobic membrane interacting region in voltage-sensing phosphatase (VSP) (2017)
  • Author(s): Akira Kawanabe, Masaki Hashimoto, Tomoko Yonezawa, Yuka Jinno, Souhei Sakata, Yasushi Okamura
  • Organizer: 第55回日本生物物理学会年会
• [Presentation] 電位依存性ホスファターゼVSPの酵素活性に対する膜近傍領域の役割 (2017)
  • Author(s): 川鍋陽、橋本真宜、米澤智子、神野有香、坂田宗平、岡村康司
  • Organizer: 平成29年度生理学研究所研究会
• [Presentation] Gene expression and behavior analysis of microglial voltage-gated proton channels in mouse (2017)
  • Author(s): Takafumi Kawai, Keizo Takao, Kenji Sakimura, Tsuyoshi Miyakawa, Yasushi Okamura
  • Organizer: 第40回日本神経科学大会
• [Presentation] Development of a chemiluminescent voltage indicator applicable to brain activity recording in freely moving multiple mice (2017)
  • Author(s): Shigenori Inagaki, Masakazu Agetsuma, Hidekazu Tsutsui, Shinya Ohara, Yoshiyuki Arai, Kazushi Suzuki, Yuka Jinno, Tomoki Matsuda, Toshio Iijima, Yasushi Okamura, Takeharu Nagai
  • Organizer: 第40回日本神経科学大会
• [Presentation] 膜の疎水領域でのリポクオリティ認識機構とナノ膜ドメインの解明 (2017)
  • Author(s): 岡村康司
  • Organizer: 新学術領域研究「脂質クオリティが解き明かす生命現象」第3回領域会議
• [Presentation] In vivo site specific labeling of the voltage-gated potassium channel Kv4.3 for structure function studies: prospects on understanding the neurodegenerative disorder spinocerebellar ataxia 19/22 at the molecular level (2017)
  • Author(s): Claudio Tiecher, Akira Kawanabe, Yasushi Okamura, Armagan Kocer
  • Organizer: Dutch Neuroscience Meeting 2017
  • Int'l Joint Research
• [Presentation] がん抑制タンパク質PTENのPIP結合の際のN末端の役割 (2017)
  • Author(s): 神田直樹, 成田宏隆, 岡村康司, 中川敦史
  • Organizer: 第16回日本蛋白質科学会年会
• [Presentation] Coupling Mechanisms of Voltage-Sensing Phosphatase (2017)
  • Author(s): Yasushi Okamura
  • Organizer: Emerging Concepts in Ion Channel Biophysics
  • Invited
• [Presentation] Atypical cation conductance through voltage sensor domain (2017)
  • Author(s): Yasushi Okamura, Hiroki Arima, Hidekazu Tsutsui
  • Organizer: Seminar on Institute of Biotechnology National Autonomous University of Mexico
  • Invited
• [Presentation] Molecular dynamics study of kinetics of the voltage-gated proton channel VSOP/Hv1 under electric fields (2017)
  • Author(s): Hiroko X. Kondo, Yasushige Yonezawa, Naoyuki Miyashita, Masayo Iwaki, Kohei Takeshita, Yuichiro Fujiwara, Matsuyuki Shirota, Kengo Kinoshita, Yasushi Okamura, Atsushi Nakagawa, Hideki Kandori, Yu Takano
  • Organizer: 第55回日本生物物理学会年会
  • Invited
• [Presentation] Structural analysis of Voltage-sensing phosphatase (VSP) on the electrochemical coupling between the Voltage sensor domain and cytoplasmic phosphatase. (2017)
  • Author(s): Hirotaka Narita, Naoki Kanda, Yasushi Okamura, Atsushi Nakagawa
  • Organizer: 第55回日本生物物理学会年会
  • Invited
• [Presentation] Structural Insight of Zinc Binding of Hv1/VSOP in Resting State (2017)
  • Author(s): Atsushi Nakagawa, Masayo Iwaki, Hideki Kandori, Hiroko Kondo, Yasushige Yonezawa, Yu Takano, Hiroki Arima, Yuichiro Fujiwara, Kohei Takeshita, Yasushi Okamura
  • Organizer: 24th Congress & General Assembly of the International Union of Crystallography 2017 (IUCr2017)
  • Invited
• [Presentation] Structural Studies of Voltage Sensing-Phosphatase (2017)
  • Author(s): Atsushi Nakagawa, Hirotaka Narita, Naoki Kanda, Akira Kawanabe, Yasushi Okamura
  • Organizer: The 2nd Molecular and Cellular Life Sciences (MCLS2017)
  • Invited
• [Presentation] Roles of membrane interaction in electrochemical coupling in voltage-sensing phosphatase (2017)
  • Author(s): Yasushi Okamura, Akira Kawanabe, Kazuhisa Nishizawa, Souhei Sakata
  • Organizer: Cold Spring Harbor Conferences Asia
  • Invited
• [Presentation] Principle and biodiversity of voltage sensor domain in channels and enzyme (2017)
  • Author(s): Yasushi Okamura
  • Organizer: Campus Asia faculty meeting
  • Place of Presentation: 大阪府吹田市
  • Int'l Joint Research / Invited
• [Presentation] 電位依存性ホスファターゼにおける膜相互作用の動態と役割 (2017)
  • Author(s): 岡村 康司、川鍋 陽、西澤 和久、中川 敦史、成田 宏隆、坂田 宗平、神野 有香
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 静岡県浜松市
  • Invited
• [Presentation] 初代培養ミクログリアにおける電位依存性プロトンチャネルのダイナミクス (2017)
  • Author(s): 河合 喬文、川村 名子、小泉 修一、阿部 学、崎村 建司、岡村 康司
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 静岡県浜松市
• [Presentation] Voltage-gated proton channel in zebrafish (DrHv1): from culture dish to living animal (2017)
  • Author(s): Adisorn Ratanayotha, Takafumi Kawai, Yasushi Okamura
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 静岡県浜松市
• [Presentation] カルシウムイオン透過性を持つ電位センサードメインの機能解析 (2017)
  • Author(s): 有馬 大貴、筒井 秀和、岡村 康司
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 静岡県浜松市
• [Presentation] マウス好中球においてHv1/VSOPはfMLPに対する走化性行動を抑制する (2017)
  • Author(s): 大河内 善史、岡村 康司
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 静岡県浜松市
• [Presentation] 電位依存性ホスファターゼVSPの新規膜相互作用部位の重要性 (2017)
  • Author(s): 川鍋 陽、橋本 真宜、米澤 智子、神野 有香、坂田 宗平、岡村 康司
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 静岡県浜松市
• [Presentation] 電位依存性ホスファターゼの構造生物学的研究 (2017)
  • Author(s): 中川 敦史、成田 宏隆、神田 直樹、川鍋 陽、岡村 康司
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 静岡県浜松市
• [Presentation] Characterization of voltage-gated proton channel in zebrafish (2016)
  • Author(s): Adisorn Ratanayota, Takafumi Kawai, Yasushi Okamura
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2016-03-22
• [Presentation] CatSperの電位センサードメインはカルシウム透過性を持つ (2016)
  • Author(s): 有馬大貴、筒井秀和、竹下浩平、中川敦史、坂本恵香、吉田学、岡村康司
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2016-03-22
• [Presentation] 非天然アミノ酸Anapで検出された電位センサーの動き (2016)
  • Author(s): 筒井秀和、神野有香、坂田宗平、岡村康司
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2016-03-22
• [Presentation] 膜脂質と相互作用する可能性のある相同領域による電位依存性ホスファターゼVSPとPTENの機能制御 (2016)
  • Author(s): 川鍋陽、神野有香、坂田宗平、岡村康司
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2016-03-22
• [Presentation] 好中球の走化性における電位依存性プロトンチャネルの役割 (2016)
  • Author(s): 大河内善史、岡村康司
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2016-03-22
• [Presentation] マウス精子における電位依存性ホスファターゼの機能 (2016)
  • Author(s): 河合喬文、宮田治彦、中西広樹、坂田宗平、有馬大貴、宮脇奈那、大河内善史、渡辺雅彦、崎村建司、佐々木雄彦、伊川正人、岡村康司
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2016-03-22
• [Presentation] CatSper has a calcium-permeable voltage sensor domain. (2016)
  • Author(s): Hiroki Arima, Hidekazu Tsutsui, Kohei Takeshita, Atsushi Nakagawa, Ayako Sakamoto, Manabu Yoshida and Yasushi Okamura
  • Organizer: The Biophysical Society 60th Annual Meeting
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2016-02-28
  • Int'l Joint Research
• [Presentation] Principle and biodiversity of voltage sensor domain proteins (2016)
  • Author(s): Yasushi Okamura
  • Organizer: Dutch Neuroscience Meeting
  • Place of Presentation: Lunteren, Netherlands
  • Int'l Joint Research / Invited
• [Presentation] How does voltage sensor domain regulate downstream effecter?: lesson from voltage-sensor domain proteins (2016)
  • Author(s): Yasushi Okamura, Souhei Sakata, Akira Kawanabe, Yuichiro Fujiwara, Yuka Jinno
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 神奈川県横浜市
  • Invited
• [Presentation] Molecular mechanisms of voltage-sensing phosphatase, VSP (2016)
  • Author(s): Yasushi Okamura
  • Organizer: 2016 International Conference of Physiological Sciences
  • Place of Presentation: 中国・北京
  • Int'l Joint Research / Invited
• [Presentation] Crossroad of electricity and lipid signaling: voltage-sensing phosphoinositide phosphatase (VSP) (2016)
  • Author(s): Yasushi Okamura, Akira Kawanabe, Souhei Sakata
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
  • Invited
• [Presentation] 電位依存性ホスファターゼVSPとPTENに共通する膜との相互作用部位の重要性 (2016)
  • Author(s): 川鍋陽、米澤智子、岡村康司
  • Organizer: 第63回日本生化学会 近畿支部例会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] 膜の疎水領域でのリポクオリティ認識機構とナノなくドメインの解明 (2016)
  • Author(s): 岡村康司
  • Organizer: 新学術領域研究「脂質クオオリティが解き明かす生命現象」第2回領域会議
  • Place of Presentation: 静岡県伊豆市
• [Presentation] Bidirectional regulation of ROS production by voltage-gated proton channels in microglia (2016)
  • Author(s): Takafumi Kawai，Yoshifumi Okochi，Tomohiko Ozaki，Yoshio Imura，Kenji Sakimura，Schuichi Koizumi，Toshihide Yamashita，Yasushi Okamura
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] The common regulation of phosphatase activity of voltage-sensing phostophase (VSP) and PTEN by conserved putatiｖe membrane interacting region (2016)
  • Author(s): Akira Kawanabe, Tomoko Yonezawa, Yuka Jinno, Souhei Sakata, Yasushi Okamura
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 宮城県仙台市
• [Presentation] CatSperの電位センサードメインはカルシウムイオン透過性を持つ (2016)
  • Author(s): 有馬大貴、筒井秀和、吉田学、岡村康司
  • Organizer: 第3回ホヤ研究会
  • Place of Presentation: 大阪府豊中市
• [Presentation] Characterization of Voltage-Gated Proton Channel in Zebrafish. (DrHv1) (2016)
  • Author(s): Adisorn Ratanayotha,Takafumi Kawai, Yasushi Okamura
  • Organizer: 第109回近畿生理学談話会
  • Place of Presentation: 大阪府大阪市
• [Presentation] 電位依存性プロトンチャネルノックアウトマウスの代謝表現型解析 (2016)
  • Author(s): 辰巳翔基、河合喬文、山本浩靖、崎村建司、木原進士、岡村康司
  • Organizer: 第109回近畿生理学談話会
  • Place of Presentation: 大阪府大阪市
• [Presentation] 細胞内膜の膜電位を可視化する－貪食細胞の食胞膜電位の測定－ (2016)
  • Author(s): 大河内善史、岡村康司
  • Organizer: 第109回近畿生理学談話会
  • Place of Presentation: 大阪府大阪市
• [Presentation] Direct observation og voltage-gated proton channels by high speed AFM (2016)
  • Author(s): Hayato Yamashita, Akira Kawanabe, Yasushi Okamura, Msayuki Abe
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: 茨城県つくば市
• [Presentation] Development of a chemiluminescent voltage indicator applicable to brain activity recording in freely moving mice (2016)
  • Author(s): Shigenori Inagaki, Masakazu Agetsuma, Hidekazu Tsutsui, Yoshiyuki Arai, Kazushi Suzuki, Yuka Jinno, Yasushi Okamura, Tomoki Matsuda, Takeharu Nagai
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: 茨城県つくば市
• [Presentation] The role of membrane interacting region of phosphatase domain in voltage-sensing phosohatase (VSP) (2016)
  • Author(s): Akira Kawanabe, Masaki Hashimoto, Tomoko Yonezawa, Yuka Jinno, Souhei Sakata, Yasushi Okamura
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: 茨城県つくば市
• [Presentation] Metal binding to the voltage-gated proton channel VSOP studied by ATR-FTIR (2016)
  • Author(s): Masayo Iwaki, Kohei takeshita, Yasushi Okamura, Atushi Nakagawa, Hideki Kandori
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: 茨城県つくば市
• [Presentation] 膜電位非印加状態で開状態をとるVSOP/Hv1変異体から見る２量体間協調性 (2016)
  • Author(s): 木村仁美、持田理子、渋村里美、中川敦史、岡村康司、竹下浩平
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] 新規電位センサータンパク質VSOP2のX線結晶構造解析に向けて (2016)
  • Author(s): 西川翔士、成田宏隆、岡村康司、中川敦史
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] 自由行動マウスの脳活動計測を可能にする化学発光膜電位センサーの開発 (2016)
  • Author(s): 稲垣成矩、揚妻正和、筒井秀和、新井由之、鈴木和志、神野有香、岡村康司、松田知己、永井健治
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] How is VSP’s enzyme activity activated by intrinsic voltage sensor? (2015)
  • Author(s): Yasushi Okamura
  • Organizer: RECI V 5th Spanish Ion Channel Network Meeting
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2015-10-04
  • Int'l Joint Research / Invited
• [Presentation] CatSper has a calcium-permeable voltage sensor domain. (2015)
  • Author(s): Hiroki Arima, Hidekazu Tsutsui and Yasushi Okamura
  • Organizer: RECI V 5th Spanish Ion Channel Network Meeting
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] 複数の光操作と組み合わせた膜電位メージングと薬剤スクリーニングの可能性を広げる発光指示薬の開発 (2015)
  • Author(s): 稲垣成矩,松田知己,新井由之,神野有香,筒井秀和,岡村康司，永井健治
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 石川県金沢市
  • Year and Date: 2015-09-13
• [Presentation] 全反射赤外分光で見る電位依存性プロトンチャネルVSOPへの金属結合 (2015)
  • Author(s): 岩城雅代, 竹下浩平, 岡村康司, 中川敦史, 神取秀樹
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 石川県金沢市
  • Year and Date: 2015-09-13
• [Presentation] カルシウムイオン透過性を持つ電位センサードメインの機能解析 (2015)
  • Author(s): 有馬大貴、筒井秀和、岡村康司
  • Organizer: 生理研研究会”膜システムの機能的・構造的統合”
  • Place of Presentation: 愛知県岡崎市
  • Year and Date: 2015-09-01
• [Presentation] Molecular mechanisms of voltage sensing phosphatase, VSP (2015)
  • Author(s): Yasushi Okamura
  • Organizer: "岡崎統合バイオサイエンスセンター サマースクール２０１５ Development of Biosensing Research"
  • Place of Presentation: 愛知県岡崎市
  • Year and Date: 2015-08-12
  • Int'l Joint Research / Invited
• [Presentation] "電位依存性プロトンチャネルによるミクログリア活性酸素産生の制御 (2015)
  • Author(s): 河合 喬文、大河内 善史、井村 誉史雄、崎村 建司、小泉 修一、岡村 康司
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2015-07-28
• [Presentation] Determinants of activation rate of voltage-gated proton channel revealed by chimeric constructs between two orthologs (2015)
  • Author(s): Yasushi Okamura
  • Organizer: TMMU Neuroscience Symposium : Ion Channels
  • Place of Presentation: 中華人民共和国、重慶
  • Year and Date: 2015-06-30
  • Int'l Joint Research / Invited
• [Presentation] 最小イオンチャネルHv1/VSOPによる貪食細胞の活性酸素産生の多重制御 (2015)
  • Author(s): 岡村康司
  • Organizer: 日本バイオイメージング学会シンポジウム
  • Place of Presentation: 東京都葛飾区
  • Invited
• [Presentation] 電位依存性プロトンチャネルの2量体と単量体間のゲーティング特性の違いを増大させる変異体の解析 (2015)
  • Author(s): 川鍋陽、岡村康司
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 石川県金沢市
• [Presentation] カルシウムイオン透過性を持つ電位センサートドメインの機能解析 (2015)
  • Author(s): 有馬大貴、筒井秀和、岡村康司
  • Organizer: 第108回近畿生理談話会
  • Place of Presentation: 大阪府東大阪市
• [Presentation] 電位依存性ホスファターゼVSPの構造基盤の解明 (2015)
  • Author(s): 岡村康司、川鍋陽、坂田宗平、筒井秀和、中川敦史、成田宏隆、鷹野優、神取秀樹
  • Organizer: ライフサイエンスの革新を目指した構造生命科学と先端的基盤技術
  • Place of Presentation: 東京都文京区
• [Book] 脂質クオリティ (2018)
  • Author(s): 岡村康司、大澤匡範
  • Total Pages: 220
  • Publisher: 羊土社
  • ISBN: 9784758103718
• [Book] ガイトン生理学 原著第13版 (2018)
  • Author(s): 石川義弘、岡村康司（総監訳）、尾仲達史、河野憲二
  • Total Pages: 1100
  • Publisher: エルゼビア・ジャパン株式会社
  • ISBN: 9784860347741
• [Book] ギャノング生理学 原書25版 (2017)
  • Author(s): 岡田泰伸、佐久間 康夫、岡村 康司 （監訳）
  • Total Pages: 920
  • Publisher: 丸善出版
  • ISBN: 9784621301883
• [Book] Voltage-gated proton channels (2017)
  • Author(s): Okamura Y.
  • Publisher: Reference Module in Life Sciences, Elsevier
  • ISBN: 9780128096338
• [Book] Reference Module in Life Sciences, 'Voltage-gated proton channels' (2017)
  • Author(s): Y. Okamura
  • Publisher: Elsevier