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Response mechanisms to a fluctuating environment through long-distance signaling

Planned Research

Project AreaIntegrative system of autonomous environmental signal recognition and memorization for plant plasticity
Project/Area Number 15H05957
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University

Principal Investigator

Matsubayashi Yoshikatsu  名古屋大学, 理学研究科, 教授 (00313974)

Co-Investigator(Kenkyū-buntansha) 望田 啓子 (桑田啓子 / 望田(桑田) 啓子)  名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (70624352)
Project Period (FY) 2015-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥95,940,000 (Direct Cost: ¥73,800,000、Indirect Cost: ¥22,140,000)
Fiscal Year 2019: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2018: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2017: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2015: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Keywordsペプチドホルモン / シロイヌナズナ / 窒素代謝 / 受容体キナーゼ / 長距離シグナリング / ペプチド / 篩管 / 窒素応答 / 硝酸 / 環境応答 / 維管束 / 窒素栄養
Outline of Final Research Achievements

Nitrogen (N) is an essential macronutrient that plays a crucial role throughout plant development, but soil nitrate availability fluctuates temporally and spatially. We identified that N starvation-induced peptide CEP acts as a root-derived ascending N-demand signal. CEP is recognized in the shoot by CEP receptor, that leads to the production of non-secreted polypeptide, CEPD. CEPD acts as a descending signal to the root and ultimately upregulates nitrate transporter NRT2.1 gene in the roots. Thus, CEP induced on one side of the roots by local N starvation is able to mediate upregulation of NRT2.1 in the distant part of the roots. In addition to this, plants modulate the efficiency of root N acquisition in response to shoot N demand by CEPD-like 2, which is upregulated in the leaf vasculature in response to decreased shoot N status. Our findings provide new insights into the molecular basis of plant adaptation to a dynamically fluctuating N environment.

Academic Significance and Societal Importance of the Research Achievements

本研究により,全身的窒素要求シグナリングの主要コンポーネントを見出したが,この発見は,植物が局所的環境受容と長距離シグナル伝達との統御に独自のメカニズムを進化させていることを示すものである.また,「維管束は単なる水や栄養の通り道ではなく,組織間を結ぶ情報伝達の場」であり,「気孔からの水分の蒸発によってできる蒸散流が,長距離シグナルの増幅や維管束内での情報伝達に重要な役割を果たしている」など,これまでの理解を覆すような新たな概念を確立した.

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (36 results)

All 2020 2019 2018 2017 2016 2015 Other

All Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 8 results,  Acknowledgement Compliant: 7 results) Presentation (20 results) (of which Int'l Joint Research: 17 results,  Invited: 17 results) Remarks (4 results)

  • [Journal Article] Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis2020

    • Author(s)
      Ota Ryosuke、Ohkubo Yuri、Yamashita Yasuko、Ogawa-Ohnishi Mari、Matsubayashi Yoshikatsu
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 641-641

    • DOI

      10.1038/s41467-020-14440-8

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Screening and identification of a non-peptide antagonist for the peptide hormone receptor in Arabidopsis.2019

    • Author(s)
      Shinohara, H., Yasue, N., Onuki, T., Yoshida, M. Matsubayashi, Y.
    • Journal Title

      Commun. Biol. Commun Biol.

      Volume: 2 Issue: 1 Pages: 61-61

    • DOI

      10.1038/s42003-019-0307-8

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploring peptide hormones in plants: identification of four peptide hormone-receptor pairs and two post-translational modification enzymes2018

    • Author(s)
      MATSUBAYASHI Yoshikatsu
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 94 Issue: 2 Pages: 59-74

    • DOI

      10.2183/pjab.94.006

    • NAID

      130006339623

    • ISSN
      0386-2208, 1349-2896
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PLENTY, a hydroxyproline O-arabinosyltransferase, negatively regulates root nodule symbiosis in Lotus japonicus2018

    • Author(s)
      Yoro Emiko、Nishida Hanna、Ogawa-Ohnishi Mari、Yoshida Chie、Suzaki Takuya、Matsubayashi Yoshikatsu、Kawaguchi Masayoshi
    • Journal Title

      Journal of Experimental Botany

      Volume: 70 Issue: 2 Pages: 507-517

    • DOI

      10.1093/jxb/ery364

    • NAID

      120007128131

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A peptide hormone required for Casparian strip diffusion barrier formation in Arabidopsis roots2017

    • Author(s)
      Nakayama T, Shinohara H, Tanaka M, Baba K, Ogawa-Ohnishi M, Matsubayashi Y.
    • Journal Title

      Science

      Volume: 355 Issue: 6322 Pages: 284-286

    • DOI

      10.1126/science.aai9057

    • NAID

      120006024333

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Shoot-to-root mobile polypeptides involved in systemic regulation of nitrogen acquisition2017

    • Author(s)
      Ohkubo Y., Tanaka M., Tabata R., Ogawa-Ohnishi M., Matsubayashi Y.
    • Journal Title

      Nature Plants

      Volume: 3 Issue: 4 Pages: 17029-17029

    • DOI

      10.1038/nplants.2017.29

    • NAID

      120006337724

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cryptic bioactivity capacitated by synthetic hybrid plant peptides2017

    • Author(s)
      Hirakawa Y, Shinohara H, Welke K, Irle S, Matsubayashi Y, Torii KU, Uchida N.
    • Journal Title

      Nature Commun.

      Volume: 8 Issue: 1 Pages: 14318-14318

    • DOI

      10.1038/ncomms14318

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis.2016

    • Author(s)
      Shinohara H, Mori A, Yasue N, Sumida K and Matsubayashi Y.
    • Journal Title

      Proc. Natl. Acad. Sci. U S A

      Volume: 印刷中 Issue: 14 Pages: 3897-3902

    • DOI

      10.1073/pnas.1522639113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Loss of function at RAE2, a novel EPFL, is required for awnlessness in cultivated Asian rice.2016

    • Author(s)
      Bessho-Uehara K, Wang DR, Furuta T, Minami A, Nagai K, Gamuyao R, Asano A, Angeles-Shim RB, Shimizu Y, Ayano M, Komeda N, Doi K, Miura K, Toda Y, Kinoshita T, Okuda S, Higashiyama T, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y, Greenberg A, Wu J, Yasui H, Yoshimura A, Mori H, McCouch SR, Ashikari M.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 113 Issue: 32 Pages: 8969-8974

    • DOI

      10.1073/pnas.1604849113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Long-distance peptide signaling essential for nutrient homeostasis in plants2016

    • Author(s)
      Okamoto S, Tabata R, Matsubayashi Y.
    • Journal Title

      Curr Opin Plant Biol

      Volume: 34 Pages: 35-40

    • DOI

      10.1016/j.pbi.2016.07.009

    • NAID

      120006024321

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A comprehensive strategy for identifying long-distance mobile peptides in xylem sap2015

    • Author(s)
      Okamoto S, Suzuki T, Kawaguchi M, Higashiyama T, Matsubayashi Y
    • Journal Title

      The Plant Journal

      Volume: 84 Issue: 3 Pages: 611-620

    • DOI

      10.1111/tpj.13015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production2015

    • Author(s)
      Tao Wu, Takehiro Kamiya, Hiroko Yumoto, Naoyuki Sotta, Yamaguchi Katsushi, Shuji Shigenobu, Yoshikatsu Matsubayashi and Toru Fujiwara
    • Journal Title

      Journal of experimental botany

      Volume: 66 Issue: 13 Pages: 3657-3667

    • DOI

      10.1093/jxb/erv105

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition.2019

    • Author(s)
      Matsubayashi Y.
    • Organizer
      23rd International Conference on Plant Growth Substances
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long distance peptide signaling mediating nitrogen homeostasis2019

    • Author(s)
      Matsubayashi Y.
    • Organizer
      18th International Workshop on Plant Membrane Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long distance peptide signaling mediating nitrogen homeostasis2019

    • Author(s)
      Matsubayashi Y.
    • Organizer
      Cold Spring Harbor Asia conference on Plant Cell and Development Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Receptor kinase signaling in plants: from peptide ligand identification to non-peptide antagonist development2019

    • Author(s)
      Matsubayashi Y.
    • Organizer
      International Symposium on Plant Receptor Kinases and Cell Signaling 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition2018

    • Author(s)
      Yoshikatsu Matsubayashi
    • Organizer
      Sainsbury Laboratory Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition2018

    • Author(s)
      Yoshikatsu Matsubayashi
    • Organizer
      Frontiers in BioAgricultural Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Root-to-shoot and shoot-to-root long-distance mobile peptides mediate systemic regulation of nitrogen acquisition2018

    • Author(s)
      Yoshikatsu Matsubayashi
    • Organizer
      SEB's Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition2018

    • Author(s)
      Matsubayashi Yoshikatsu
    • Organizer
      Sainsbury Laboratory Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition2018

    • Author(s)
      Matsubayashi Yoshikatsu
    • Organizer
      Frontiers in BioAgricultural Research
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Root-to-shoot and shoot-to-root long-distance mobile peptides mediate systemic regulation of nitrogen acquisition2018

    • Author(s)
      Matsubayashi Yoshikatsu
    • Organizer
      SEB's Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Root-to-shoot and shoot-to-root long-distance mobile peptides mediate systemic regulation of nitrogen acquisition2018

    • Author(s)
      Matsubayashi Yoshikatsu
    • Organizer
      Keystone Symposia, Plant Signaling: Molecular Pathways and Network Integration
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of novel peptide ligand-receptor pairs in plants2016

    • Author(s)
      Matsubayashi Y.
    • Organizer
      Cold Spring Harbor Asia conference on Latest Advances in Plant Development & Environmental Responses
    • Place of Presentation
      Awaji, Japan
    • Year and Date
      2016-11-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance mobile peptides mediate systemic nitrogen-demand signaling2016

    • Author(s)
      Matsubayashi Y.
    • Organizer
      The 4th European Workshop on Peptide Signalling in Plants
    • Place of Presentation
      Bischoffsheim, France
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance mobile peptides mediate systemic nitrogen-demand signaling2016

    • Author(s)
      Matsubayashi Y.
    • Organizer
      EMBO conference ‘The nitrogen nutrition of plants - Nitrogen2016’
    • Place of Presentation
      Montpellier, France
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Root-to-shoot mobile peptides mediate systemic nitrogen-demand signaling2016

    • Author(s)
      Matsubayashi Y.
    • Organizer
      Plant Biology 2016
    • Place of Presentation
      Austin, Texas
    • Year and Date
      2016-07-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of novel peptide ligand-receptor pairs in plants2016

    • Author(s)
      Matsubayashi Y.
    • Organizer
      The 27th International Conference on Arabidopsis Research (ICAR)
    • Place of Presentation
      Gyeong Ju, Korea
    • Year and Date
      2016-07-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of novel peptide hormones in plants2016

    • Author(s)
      Matsubayashi Y.
    • Organizer
      The 22nd International Plant Growth Substances Association (IPGSA) conference
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2016-06-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 窒素欠乏時における植物の長距離シグナル2016

    • Author(s)
      田畑亮,松林嘉克
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] TPST deficient mutant reveals the involvement of sulfated-peptide signaling in protonema and gametophore development in Physcomitrella patens2016

    • Author(s)
      垣田満,壁谷幸子,長谷部光泰,松林 嘉克
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] アラビノガラクタンプロテイン生 合成における鍵酵素の同定と解析2016

    • Author(s)
      小川(大西)真理,川本剛,松林嘉克
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-15
    • Related Report
      2015 Annual Research Report
  • [Remarks] 名古屋大学 大学院理学研究科 生命理学専攻 細胞間シグナル研究グループ(松林嘉克教授)ホームページ

    • URL

      http://www.bio.nagoya-u.ac.jp/~b2/index.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 名古屋大学 大学院理学研究科 生命理学専攻 細胞間シグナル研究グループ(松林嘉克教授)ホームページ

    • URL

      http://www.bio.nagoya-u.ac.jp/~b2/index.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] 名古屋大学大学院理学研究科生命理学専攻|細胞間シグナル研究グループ

    • URL

      http://www.bio.nagoya-u.ac.jp/~b2/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 名古屋大学 大学院理学研究科 生命理学専攻 細胞間シグナル研究グループ ホームページ

    • URL

      http://www.bio.nagoya-u.ac.jp/~b2/

    • Related Report
      2015 Annual Research Report

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Published: 2015-07-02   Modified: 2022-11-04  

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