Environmental control of plant cell reprogramming

• Project Area: Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
• Project/Area Number: 15H05961
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: SUGIMOTO Keiko 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (30455349)
• Project Period (FY): 2015-06-29 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥92,040,000 (Direct Cost: ¥70,800,000、Indirect Cost: ¥21,240,000); Fiscal Year 2019: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2018: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2017: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2016: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2015: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
• Keywords: エピジェネティックス / ヒストン修飾 / 細胞リプログラミング / 傷害ストレス / シロイヌナズナ / 全能性 / 細胞記憶

Outline of Final Research Achievements

Some plant cells in somatic tissues dedifferentiate and redifferentiate in response to various environmental stimuli but molecular mechanisms underlying this control are not well understood. In this study we studied how plants transduce the severe damage signals and modify epigenetic cellular memories to induce cellular reprogramming. We demonstrated that histone acetylation is central to promote cellular reprgramming in plants.

Academic Significance and Societal Importance of the Research Achievements

環境刺激によって個々の細胞の記憶が可塑的に制御される分子機構を明らかにすることで、植物の多元的な環境応答システムの理解を進めることができる点に学術的意義がある。これまで再生が困難であった植物種でも、ヒストンのアセチル化を人為的に操作することで効率よく細胞リプログラミングを誘導し、再生しやすくすることが期待される。

Research Products

• [Int'l Joint Research] Sainsbury Lab Cambridge University(英国)
• [Int'l Joint Research] Sainsbury Lab Cambridge University(英国)
• [Int'l Joint Research] University of California, Davis(米国)
• [Int'l Joint Research] Sainsbury Lab Cambridge University(英国)
• [Int'l Joint Research] Sainsbury Lab Cambridge University(United Kingdom)
• [Int'l Joint Research] IISER(India)
• [Journal Article] AT-Hook Transcription Factors Restrict Petiole Growth by Antagonizing PIFs (2020)
  • Author(s): Favero David S.、Kawamura Ayako、Shibata Michitaro、Takebayashi Arika、Jung Jae-Hoon、Suzuki Takamasa、Jaeger Katja E.、Ishida Takashi、Iwase Akira、Wigge Philip A.、Neff Michael M.、Sugimoto Keiko
  • Journal Title: Current Biology Volume: 30 Pages: 1454-1466
  • DOI: 10.1016/j.cub.2020.02.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Histone acetylation orchestrates wound-induced transcriptional activation and cellular reprogramming in Arabidopsis (2019)
  • Author(s): Rymen Bart、Kawamura Ayako、Lambolez Alice、Inagaki Soichi、Takebayashi Arika、Iwase Akira、Sakamoto Yuki、Sako Kaori、Favero David S.、Ikeuchi Momoko、Suzuki Takamasa、Seki Motoaki、Kakutani Tetsuji、Roudier Fran?ois、Sugimoto Keiko
  • Journal Title: Communications Biology Volume: 2 Pages: 404-404
  • DOI: 10.1038/s42003-019-0646-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Mechanisms of Plant Regeneration (2019)
  • Author(s): Ikeuchi Momoko、Favero David S.、Sakamoto Yuki、Iwase Akira、Coleman Duncan、Rymen Bart、Sugimoto Keiko
  • Journal Title: Annual Review of Plant Biology Volume: 70 Pages: 377-406
  • DOI: 10.1146/annurev-arplant-050718-100434
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A gene regulatory network for root hair development (2019)
  • Author(s): Michitaro Shibata, Keiko Sugimoto
  • Journal Title: Journal of Plant Research Volume: 132 Pages: 301-309
  • DOI: 10.1007/s10265-019-01100-2
  • Peer Reviewed / Open Access
• [Journal Article] WIND1 induces dynamic metabolomic reprogramming during regeneration in Brassica napus (2018)
  • Author(s): Akira Iwase, Kento Mita, David S.Favero, Nobutaka Mitsuda, Ryosuke Sasaki, Makoto Kobayshi, Yumiko Takebayashi, Mikiko Kojima, Miyako Kusano, Akira Oikawa, Hitoshi Sakakibara, Kazuki Saito, Jun Imamura, Keiko Sugimoto
  • Journal Title: Developmental Biology Volume: 442 Pages: 40-52
  • DOI: 10.1016/j.ydbio.2018.07.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Gene Regulatory Network for Cellular Reprogramming in Plant Regeneration (2018)
  • Author(s): Ikeuchi M, Shibata M, Rymen B, Iwase A, Bagman AM, Watt L, Coleman D, Favero DS, Takahashi T, Ahnert SE, Brady SM, Sugimoto K
  • Journal Title: Plant and Cell Physiology Volume: 59 Pages: 770-782
  • DOI: 10.1093/pcp/pcy013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] GTL1 and DF1 regulate root hair growth through transcriptional repression of ROOT HAIR DEFECTIVE 6-LIKE 4 in Arabidopsis (2018)
  • Author(s): Shibata Michitaro、Breuer Christian、Kawamura Ayako、Clark Natalie M.、Rymen Bart、Braidwood Luke、Morohashi Kengo、Busch Wolfgang、Benfey Philip N.、Sozzani Rosangela、Sugimoto Keiko
  • Journal Title: Development Volume: 145 Pages: 159707-159707
  • DOI: 10.1242/dev.159707
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes (2017)
  • Author(s): Ikeuchi M, Iwase A, Rymen B, Lambolez A, Kojima M, Takebayashi Y, Heyman J, Watanabe S, Seo M, De Veylder L, Sakakibara H, Sugimoto K
  • Journal Title: Plant Physiology Volume: 175 Pages: 1158-1174
  • DOI: 10.1104/pp.17.01035
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] WIND1 promotes shoot regeneration through transcriptional activation of ESR1 in Arabidopsis. (2017)
  • Author(s): 1.Iwase A, Harashima H, Ikeuchi M, Rymen B, Ohnuma M, Komaki S, Morohashi K, Kurata T, Nakata M, Ohme-Takagi M, Grotewold E, Sugimoto K
  • Journal Title: The Plant Cell Volume: 1 Pages: 54-69
  • DOI: 10.1105/tpc.16.00623
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular networks orchestrating plant cell growth. (2017)
  • Author(s): Franciosini, A., Rymen, B., Shibata, M., Favero, D.S., and Sugimoto, K.
  • Journal Title: Curr Opin Plant Biol Volume: 35 Pages: 98-104
  • DOI: 10.1016/j.pbi.2016.11.010
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The heterodimeric transcription factor complex ERF115-PAT1 grants regeneration competence (2016)
  • Author(s): Heyman J, Cools T, Shavialenka S, Canher B, Traas J, Vercauteren I, Van den Daele H, Persiau G, De Jaeger G, Sugimoto K, De Veylder L.
  • Journal Title: Nature Plants Volume: 2 Pages: 16165-16165
  • DOI: 10.1038/nplants.2016.165
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptional Regulation of Arabidopsis Polycomb Repressive Complex 2 Coordinates Cell-Type Proliferation and Differentiation (2016)
  • Author(s): de Lucas Miguel、Pu Li、Turco Gina、Gaudinier Allison、Morao Ana Karina、Harashima Hirofumi、Kim Dahae、Ron Mily、Sugimoto Keiko、Roudier Francois、Brady Siobhan M.
  • Journal Title: The Plant Cell Volume: 28 Pages: 2616-2631
  • DOI: 10.1105/tpc.15.00744
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Plant regeneration: cellular origins and molecular mechanisms (2016)
  • Author(s): Momoko Ikeuchi, Yoichi Ogawa, Akira Iwase, Keiko Sugimoto
  • Journal Title: Development Volume: 143 Pages: 1442-1451
  • DOI: 10.1242/dev.134668
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis. (2015)
  • Author(s): Ikeuchi M, Iwase A, Rymen B, Harashima H, Shibata M, Ohnuma M, Breuer C, Morao AK, de Lucas M, De Veylder L., Goodrich J, Brady SM, Roudier F, Sugimoto K.
  • Journal Title: Nature Plants Volume: 1 Pages: 15089-15089
  • DOI: 10.1038/nplants.2015.89
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed (2015)
  • Author(s): Iwase A,Mita K,Nonaka S, Ikeuchi M, Koizuka C, Ohnuma M, Ezura H, Imamura J, Sugimoto K
  • Journal Title: J of Plant Res Volume: 128 Pages: 389-397
  • DOI: 10.1007/s10265-015-0714-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Control of plant cell differentiation by histone modification and DNA methylation (2015)
  • Author(s): Ikeuchi M, Iwase A, Sugimoto K
  • Journal Title: Curr Opin in Plant Biol Volume: 28 Pages: 60-67
  • DOI: 10.1016/j.pbi.2015.09.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Plant Cell Reprogramming as an adaptive strategy. (2015)
  • Author(s): Sugimoto K
  • Journal Title: J Plant Res Volume: 128(3) Pages: 345-347
  • DOI: 10.1007/s10265-015-0718-7
  • Peer Reviewed
• [Presentation] Epigenetic control of cellular reprogramming in plants (2019)
  • Author(s): Keiko Sugimoto
  • Organizer: principles of pluripotent stem cells underlying plant vitality, Sendai Japan
  • Int'l Joint Research / Invited
• [Presentation] Epigenetic control of cellular reprogramming in plants (2019)
  • Author(s): Keiko Sugimoto
  • Organizer: International Conference on Arabidopsis Research, Wuhan, China
  • Int'l Joint Research / Invited
• [Presentation] 植物の環境記憶と細胞リプログラミング (2019)
  • Author(s): 杉本 慶子
  • Organizer: 日本植物学会年会シンポジウム、仙台
• [Presentation] Molecular mechanism of cellular reprogramming in plants (2019)
  • Author(s): Keiko Sugimoto
  • Organizer: University of Geneva, Switzerland
  • Invited
• [Presentation] Molecular mechanism of cellular reprogramming in plants (2019)
  • Author(s): Keiko Sugimoto
  • Organizer: ENS Lyon, France
  • Invited
• [Presentation] Transcriptional control of stress-induced cellular reprogramming in plants (2019)
  • Author(s): Keiko Sugimoto
  • Organizer: Cold Spring Harbor Asia Meeting, Gyeongyu, Korea
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanism of cellular reprogramming in plants (2019)
  • Author(s): Keiko Sugimoto
  • Organizer: Academia Sinica, Taipei, Taiwan
  • Invited
• [Presentation] Cellular reprogramming in plant regeneration (2017)
  • Author(s): Sugimoto Keiko
  • Organizer: Canada-Japan Joint Meeting on Genetic Network and Cellular Wiring
  • Int'l Joint Research / Invited
• [Presentation] Cellular reprogramming in plant regeneration (2017)
  • Author(s): 杉本慶子
  • Organizer: 第50回日本発生生物学会大会シンポジウム
  • Int'l Joint Research
• [Presentation] Epigenetic control of plant cellular reprogramming (2017)
  • Author(s): Sugimoto K
  • Organizer: Joint Meeting for German and Japanese Societies of Developmental Biologists
  • Int'l Joint Research / Invited
• [Presentation] Epigenetic control of plant cellular reprogramming (2016)
  • Author(s): Sugimoto K
  • Organizer: Cold Spring Harbor Asia Conference
  • Int'l Joint Research / Invited
• [Presentation] Totipotency: the plant’s view (2016)
  • Author(s): Sugimoto K
  • Organizer: Lausanne Genomics Days
  • Invited
• [Presentation] Totipotency: the plant’s view. (2016)
  • Author(s): Keiko Sugimoto
  • Organizer: Lausanne Genomics Days
  • Place of Presentation: University of Lausanne, Lausanne Switzerland
  • Int'l Joint Research / Invited
• [Presentation] Plant cell reprograming at the right time and place (2015)
  • Author(s): Keiko Sugimoto
  • Organizer: IPMB conference
  • Place of Presentation: Bourbon Cataratas Convention, Igauazu Falls,Brazil
  • Int'l Joint Research / Invited
• [Presentation] Plant cell reprograming at the right time and place (2015)
  • Author(s): Keiko Sugimoto
  • Organizer: ERATO symposium
  • Place of Presentation: 名古屋大学、愛知県名古屋市
  • Int'l Joint Research / Invited
• [Remarks] 細胞機能研究チーム
  • URL: http://cellfunction.riken.jp/
• [Remarks] 細胞機能研究チーム
  • URL: http://cellfunction.riken.jp/index.html
• [Remarks] Cell Function Research Team
  • URL: http://cellfunction.riken.jp/index.html
• [Remarks] Cell Function Research Team
  • URL: http://cellfunction.riken.jp/