| Budget Amount *help |
¥84,760,000 (Direct Cost: ¥65,200,000、Indirect Cost: ¥19,560,000)
Fiscal Year 2019: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2018: ¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
Fiscal Year 2017: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2016: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2015: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Outline of Final Research Achievements |
Reflecting environmental conditions, ON/OFF states of gene activity are memorized on chromatin in the forms of DNA and histone modifications. These modifications are found in both promoters in internal regions of genes (gene bodies), but role of gene body modifications remains largely unexplored. We examined factors controlling gene body modifications by genetic approaches. Arabidopsis mutations of the histone demethylase gene IBM1 induces accumulation of silent mark H3K9me (methylation of histone H3 lysine 9) in gene bodies as well as developmental abnormalities and defense responses. Genetic screening of suppressor of the ibm1 mutation identified another histone demethylase gene LDL2. LDL2 mediates developmental abnormalities through removal of H3K4me1. A related H3K4 demethylase FLD mediates memory of cold by removing H3K4me1 around transcription terminations sites; and this is co-opted from the global mechanism to control bidirectional transcription.
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