| Budget Amount *help |
¥126,620,000 (Direct Cost: ¥97,400,000、Indirect Cost: ¥29,220,000)
Fiscal Year 2019: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2018: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2017: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2016: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2015: ¥33,800,000 (Direct Cost: ¥26,000,000、Indirect Cost: ¥7,800,000)
|
|---|
| Outline of Final Research Achievements |
We aimed to elucidate the molecular principle of homologous recombination-mediated DNA double-strand break repair in vitro. In the early stage of homologous recombination, the DNA double-strand break end is processed by Mre11 nuclease to produce 3' protruded ssDNA, which becomes a substrate of Rad51 protein. The Rad51 protein binds to the single-stranded DNA. The resultant presynaptic filament initiates the homology search and catalyzes strand exchange with the double-stranded DNA serving as the donor. These reactions were analyzed using an in vitro reconstitution system consisting of highly purified protein. We have elucidated that the molecular mechanisms by which the Ctp1 protein activates Mre11 and by which Rad51 promotes the DNA strand exchange reaction in three steps.
|
