| Budget Amount *help |
¥124,540,000 (Direct Cost: ¥95,800,000、Indirect Cost: ¥28,740,000)
Fiscal Year 2019: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2018: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2017: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2016: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2015: ¥31,720,000 (Direct Cost: ¥24,400,000、Indirect Cost: ¥7,320,000)
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| Outline of Final Research Achievements |
The in vitro DNA replication system was reconstituted from budding yeast purified proteins and optimized for analyses of chromosome dynamics. Using this reconstituted system, we have revealed a novel molecular mechanism of replication-origin loading of the pre-replicative complex at the initiation step of DNA replication, contribution of the interaction between DNA polymerase epsilon and GINS, a component of replicative helicase, to chromosomal DNA synthesis, and functional implication of the heterotetramer structure of the Sld3-Sld7 complex working for the initiation of DNA replication. We also established the in vitro system for pausing the replication forks and showed that DNA polymerase epsilon functions for pausing the replication fork.
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