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ウイルス潜在感染による植物への環境ストレス耐性付与と生態系の恒常性維持の基盤解析

Planned

Project AreaNeo-virology: the raison d'etre of viruses
Project/Area Number 16H06435
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTohoku University

Principal Investigator

高橋 英樹  東北大学, 農学研究科, 教授 (20197164)

Co-Investigator(Kenkyū-buntansha) 福原 敏行  東京農工大学, (連合)農学研究科(研究院), 教授 (90228924)
Project Period (FY) 2016-06-30 – 2021-03-31
Project Status Granted (Fiscal Year 2020)
Budget Amount *help
¥117,650,000 (Direct Cost: ¥90,500,000、Indirect Cost: ¥27,150,000)
Fiscal Year 2020: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2019: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2018: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2017: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2016: ¥29,770,000 (Direct Cost: ¥22,900,000、Indirect Cost: ¥6,870,000)
Keywords不顕性感染 / 植物ウイルス / シトシン脱メチル化 / ウイルス / ウィルス / 植物 / 環境
Outline of Annual Research Achievements

近年の研究から、自然界の様々な植物の中には、ゲノム上にウイルスゲノムと相同の塩基配列を保有したり、ウイルスの潜在・不顕性感染を受けたりしていることが明らかになってきた。しかし、地球生態系に存在する約30万種の植物の生命活動におけるこれらのウイルスの役割は、まったく未知である。
本計画研究では、病原体としてのウイルスという発想を転換し、自然界の野生植物や栽培作物に明瞭な病徴を示さずに感染しているウイルスに焦点をあて、宿主植物、ウイルス、内生菌・根圏生息菌群集を包括して超植物体として捉え、植物の生命活動を制御するウイルスの役割とその具体的な分子基盤を解明する。さらに、領域内の他研究班やユニットとの有機的連携により、生態系における新たなウイルス研究の創造が加速化させる。これまでの研究から、ハクサンハタザオ(Arabidopsis halleri)から単離されたキュウリモザイクウイルス(CMV)Ho系統は、シロイヌナズナ(A.thaliana)不顕性感染することを見出している。これまでの病原性系統CMV(Y)とCMV(Ho)の比較解析から、(1)CMVの2bタンパク質の2アミノ酸置換が、CMV(Ho)のシロイヌナズナへの不顕性感染に関与していること、(2)2bタンパク質が、宿主ゲノムDNAのメチル化/脱メチル化を制御するAGO4タンパク質と直接結合することを見出した。本年度は、CMV(Ho)が不顕性感染したシロイヌナズナ、および2bタンパク質遺伝子を形質転換したシロイヌナズナにおけるゲノムDNA全体のメチル化/脱メチル化レベルと、コントロール植物におけるとメチル化/脱メチル化レベルを比較解析する。さらに、CMV(Ho)不顕性感染シロイヌナズナでメチル化/脱メチル化レベルが変動する遺伝子を同定する。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

2bタンパク質の形質転換体を作出したところ、当初の想定に反して、遺伝子が発現していないことが判明したが、研究計画を見直し、2bタンパク質発現ベクターの構造の確認と、形質転換体の作出方法の検討を行い、2bタンパク質を発現する形質転換体を得ることが出来た。さらに同形質転換体のゲノムシトシンの脱メチル化の解析を行い、プロモーター領域の脱メチル化が促進されている82遺伝子を同定できたことから、研究はおおむね順調に進展している。

Strategy for Future Research Activity

メチル化と脱メチル化変動遺伝子の翻訳産物の量的変動解析を完了させ、研究の取りまとめを行う予定である。

Report

(4 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report

Research Products

(60 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (20 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 20 results,  Open Access: 14 results) Presentation (36 results) (of which Int'l Joint Research: 10 results,  Invited: 9 results) Book (2 results) Funded Workshop (1 results)

  • [Int'l Joint Research] ワーゲニンゲン大学/ユトレヒト大学(オランダ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Single amino acid substitutions in the cucumber mosaic virus 1a protein induce necrotic cell death in virus-inoculated leaves without affecting virus multiplication.2020

    • Author(s)
      Tian A, Miyashita S, Ando S, Takahashi H
    • Journal Title

      Viruses

      Volume: 12 Pages: 91-91

    • DOI

      10.3390/v12010091

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect on tomato plants of asymptomatic infection with southern tomato virus.2020

    • Author(s)
      Fukuhara T, Tabara M, Koiwa H, Takahashi H
    • Journal Title

      Archives of Virology

      Volume: 165 Pages: 11-20

    • DOI

      10.1007/s00705-019-04436-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nasal priming with immunobiotic lactobacilli improves the adaptive immune response against influenza virus.2020

    • Author(s)
      Raya F, Islam MA, Vizoso-Pinto MG, Takahashi H, Kitazawa H, Villena J
    • Journal Title

      International Immunopharmacology

      Volume: 78 Pages: 106115-106115

    • DOI

      10.1016/j.intimp.2019.106115

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Virus latency and the impact on plants.2019

    • Author(s)
      Takahashi H, Fukuhara T, Kitazawa H, Kormelink R
    • Journal Title

      Frontiers in Microbiology

      Volume: 10 Pages: 2764-2764

    • DOI

      10.3389/fmicb.2019.02764

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of the Immunomodulatory Activities of the Probiotic Strain Lactobacillus fermentum UCO-979C2019

    • Author(s)
      Garcia-Castillo Valeria、Komatsu Ryoya、Clua Patricia、Indo Yuhki、Takagi Michihiro、Salva Susana、Islam Md. Aminul、Alvarez Susana、Takahashi Hideki、Garcia-Cancino Apolinaria、Kitazawa Haruki、Villena Julio
    • Journal Title

      Frontiers in Immunology

      Volume: 10 Pages: 1376-1376

    • DOI

      10.3389/fimmu.2019.01376

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation2019

    • Author(s)
      Igata Manami、Islam Md. Aminul、Tada Asuka、Takagi Michihiro、Kober A. K. M. Humayun、Albarracin Leonardo、Aso Hisashi、Ikeda-Ohtsubo Wakako、Miyazawa Kenji、Yoda Kazutoyo、He Fang、Takahashi Hideki、Villena Julio、Kitazawa Haruki
    • Journal Title

      Frontiers in Immunology

      Volume: 10 Pages: 1180-1194

    • DOI

      10.3389/fimmu.2019.01180

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Endornaviruses: persistent dsRNA viruses with symbiotic properties in diverse eukaryotes.2019

    • Author(s)
      Fukuhara T
    • Journal Title

      Virus Genes

      Volume: 55 Pages: 165-173

    • DOI

      10.1007/s11262-019-01635-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long DCL4-substrate dsRNAs efficiently induce RNA interference in plant cells.2019

    • Author(s)
      Kakiyama S, Tabara M, Nishibori Y, Moriyama H, Fukuhara T.
    • Journal Title

      Scientific Reports

      Volume: in press Pages: 1-10

    • DOI

      10.1038/s41598-019-43443-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Deciphering the influence of paraimmunobiotic bifidobacteria on the innate antiviral immune response of bovine intestinal epitheliocytes by transcriptomic analysis2019

    • Author(s)
      Albarracin L.、Komatsu R.、Garcia-Castillo V.、Aso H.、Iwabuchi N.、Xiao J.-Z.、Abe F.、Takahashi H.、Villena J.、Kitazawa H.
    • Journal Title

      Beneficial Microbes

      Volume: 10 Pages: 199-209

    • DOI

      10.3920/bm2018.0024

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Long DCL4-substrate dsRNAs efficiently induce RNA interference in plant cells2019

    • Author(s)
      Kakiyama S, Tabara M, Nishibori Y, Moriyama H, Fukuhara T.
    • Journal Title

      Scientific Reports

      Volume: 9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] First identification and characterization of cucumber mosaic virus from Corchorus olitorius in Japan2018

    • Author(s)
      Hanbal Sara E.、Miyashita Shuhei、Ando Sugihiro、Sidaros Samir A.、Takahashi Hideki
    • Journal Title

      Journal of Plant Pathology

      Volume: 100 Pages: 561-565

    • DOI

      10.1007/s42161-018-0099-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity2018

    • Author(s)
      Hanbal Sara E.、Takashima Keisuke、Miyashita Shuhei、Ando Sugihiro、Ito Kumiko、Elsharkawy Mohsen M.、Kaneko Toshiro、Takahashi Hideki
    • Journal Title

      Archives of Virology

      Volume: 163 Pages: 2835-2840

    • DOI

      10.1007/s00705-018-3909-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Survey of the response of 82 domestic landraces of Zea mays to cucumber mosaic virus reveals geographical region-related resistance to CMV in Japan2018

    • Author(s)
      Takahashi H.、Tian A.、Miyashita S.、Kanayama Y.、Ando S.、Kormelink R.
    • Journal Title

      Plant Pathology

      Volume: in press Pages: 1401-1415

    • DOI

      10.1111/ppa.12848

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Receptors and signaling pathways for recognition of bacteria in livestock and crops: prospects for beneficial microbes in healthy growth strategies2018

    • Author(s)
      Villena, J., H. Kitazawa, S. C. M. Van Wees, C. M. J. Pieterse, H. Takahashi
    • Journal Title

      Frontiers in Immunology

      Volume: 9:2223 Pages: 1-15

    • DOI

      10.3389/fimmu.2018.02223

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative analysis of microbial diversity and bacterial seedling disease-suppressive activity in organic-farmed and standardized commercial conventional soils for rice nursery cultivation2018

    • Author(s)
      Takahashi Hideki、Matsushita Yuko、Ito Toyoaki、Nakai Yutaka、Nanzyo Masami、Kobayashi Takashi、Iwaishi Shinji、Hashimoto Tomoyoshi、Miyashita Shuhei、Morikawa Toshiyuki、Yoshida Shigenobu、Tsushima Seiya、Ando Sugihiro
    • Journal Title

      Journal of Phytopathology

      Volume: 166 Pages: 249264-249264

    • DOI

      10.1111/jph.12682

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic characterization of Lactobacillus delbrueckii TUA4408L and evaluation of the antiviral activities of its extracellular polysaccharides in porcine intestinal epithelial cells2018

    • Author(s)
      Kanmani, P., L. Albarracin, H. Kobayashi, E. M. Hebert, L. Saavedra, R. Komatsu, B. Gatica, A. S. Burgos, A. Miyazaki, W. Ikeda-Ohtsubo, Y. Suda, H. Aso, S. Egusa, T. Mishima, H. Takahashi, J. Villena, H. Kitazawa
    • Journal Title

      Frontiers in Immunology

      Volume: 9:2178 Pages: 1-16

    • DOI

      10.3389/fimmu.2018.02178

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Immunobiotics for the bovine host: their interaction with intestinal epithelial cells and their effect on antiviral immunity.2018

    • Author(s)
      Julio Villena, Hisashi Aso, Victor P. Rutten, Hideki Takahashi, Willem Van Eden and Haruki Kitazawa
    • Journal Title

      Front. Immunol.

      Volume: 9 Pages: 1-10

    • DOI

      10.3389/fimmu.2018.00326

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Size distribution of small interfering RNAs in various organs at different developmental stages is primarily determined by the dicing activity of Dicer-like proteins in plants2018

    • Author(s)
      Tabara M, Ohtani M, Kanekatsu M, Moriyama H, Fukuhara T
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Pages: 2228-2238

    • DOI

      10.1093/pcp/pcy144

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased cytosine methylation at promoter of the NB-LRR class R gene RCY1 correlated with compromised resistance to cucumber mosaic virus in EMS-generated src mutants of Arabidopsis thaliana2017

    • Author(s)
      Sato Yukiyo、Miyashita Shuhei、Ando Sugihiro、Takahashi Hideki
    • Journal Title

      Physiological and Molecular Plant Pathology

      Volume: 100 Pages: 151-162

    • DOI

      10.1016/j.pmpp.2017.09.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Respiratory Commensal Bacteria Corynebacterium pseudodiphtheriticum Improves Resistance of Infant Mice to Respiratory Syncytial Virus and Streptococcus pneumoniae Superinfection2017

    • Author(s)
      Kanmani Paulraj、Clua Patricia、Vizoso-Pinto Maria G.、Rodriguez Cecilia、Alvarez Susana、Melnikov Vyacheslav、Takahashi Hideki、Kitazawa Haruki、Villena Julio
    • Journal Title

      Front. Microbiol.

      Volume: 8 Pages: 1-14

    • DOI

      10.3389/fmicb.2017.01613

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] キュウリモザイクウイルスに不顕性感染したシロイヌナズナにおける2bタンパク質の役割2019

    • Author(s)
      高橋英樹・宮下脩平・田原 緑・栗山 和典・安藤杉尋・福原敏行
    • Organizer
      令和元年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] キュウリモザイクウイルス(CMV)に対するエジプト産ササゲ品種EGの応答とCMVに対する高度抵抗性の性状解析2019

    • Author(s)
      井尾美由記・村山友理・宮下脩平・安藤杉尋・Sara E. Hanbal・高橋英樹
    • Organizer
      令和元年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] エジプト産ササゲ品種EGのキュウリモザイクウイルス高度抵抗性を決定するウイルス因子の解析2019

    • Author(s)
      村山友理・井尾美由記・安藤杉尋・宮下脩平・Sara E. Hanbal・高橋英樹
    • Organizer
      令和元年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Analysis of genomic region in cucumber mosaic virus RNA1 which determines the induction of the non-HR cell death in reassorted CMV-inoculated leaves of Arabidopsis thaliana2019

    • Author(s)
      Ainan Tian・Shuhei Miyashita・Sugihiro Ando・Hideki Takahashi
    • Organizer
      令和元年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] BTH処理によるRNAサイレンシング関連因子AGO2のプライミングを制御する転写因子の探索2019

    • Author(s)
      安藤杉尋・小関彩恵子・宮下脩平・高橋英樹
    • Organizer
      令和元年度日本植物病理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 星咲き品種ペチュニアにおける内在性ウイルスの活性化とそのRNA干渉への影響の解析2019

    • Author(s)
      栗山和典、田原緑、高橋英樹、福原敏行
    • Organizer
      第8回植物RNA研究者ネットワークシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 野生植物ハクサンハタザオのウイルス抵抗性と環境ストレス耐性2019

    • Author(s)
      福原敏行、田原緑、高橋英樹
    • Organizer
      第8回植物RNA研究者ネットワークシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Virus latency and the impact on plants2019

    • Author(s)
      Hideki Takahashi
    • Organizer
      The 18th Awaji International Forum on Infection and Immunity
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Introduction of International Education and Research Center for Food and Agricultural Immunology2019

    • Author(s)
      Hideki Takahashi
    • Organizer
      International Symposium on New Insights on Animal Nutrition, Breeding and Reproduction
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A simple evolution model of MOI unveils the distinct biological principles behind different classes of viruses2019

    • Author(s)
      Shuhei Miyashita
    • Organizer
      International Neovirology Mini-symposium Series IV
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] キュウリモザイクウイルスに不顕性感染したシロイヌナズナにおける2bタンパク質の役割2019

    • Author(s)
      高橋英樹・田原 緑・宮下脩平・安藤杉尋・福原敏行
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity2019

    • Author(s)
      Sara E. Hanbal・Keisuke Takashima・Shuhei Miyashita・Sugihiro Ando・Kumiko Ito・Mohsen M. Elsharkawy・Toshiro Kaneko・Hideki Takahashi
    • Organizer
      令和元年度日本植物病理学会東北部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] An endogenous Petunia vein clearing virus affects RNA interference in the star-type petunia2019

    • Author(s)
      Kazunori Kuriyama, Midori Tabara, Hideki Takahashi, Hiromitsu Moriyama and Toshiyuki Fukuhara
    • Organizer
      Post-transcriptional Gene Regulation in Plants 2019
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] キュウリモザイクウイルスに無病徴感染したシロイヌナズナにおけるRNA-seq解析2018

    • Author(s)
      高橋英樹・田原 緑・平山裕也・宮下脩平・安藤杉尋・川野修一・福原敏行
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナへの無病徴感染に関わるキュウリモザイクウイルスのゲノムRNAと宿主因子の解析2018

    • Author(s)
      吉澤 峻・宮下脩平・安藤杉尋・福原敏行・高橋英樹
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キュウリモザイクウイルスに不顕性感染したシロイヌナズナにおけるダイコンモザイクウイルスとTurnip crinkle virusの増殖抑制2018

    • Author(s)
      八木橋素良・宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      平成30年度日本植物病理学会東北部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Latent plant-virus interactions and their impact on plant stress tolerance2018

    • Author(s)
      Hideki Takahashi
    • Organizer
      WUR-TU Plant Science Workshop 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of genomic region in cucumber mosaic virus RNA1 which determines ectopic induction of cell death in reassorted CMV-inoculated leaves of Arabidopsis thaliana2018

    • Author(s)
      Ainan Tian, Shuhei Miyashita, Sugihiro Ando, and Hideki Takahashi
    • Organizer
      平成30年度日本植物病理学会東北部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Latent plant-virus interactions and their impact on plant stress tolerance.2018

    • Author(s)
      Hideki Takahashi
    • Organizer
      WUR-TU Plant Science Workshop 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Social aspects in viral ways of life, analyzed by wet experiments and dry simulations.2018

    • Author(s)
      Shuhei Miyashita
    • Organizer
      WUR-TU Plant Science Workshop 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Evolution of strategies in space: the never-ending quests for higher fitness by viruses and the hosts driven by differences and changes in spatial structures.2018

    • Author(s)
      Shuhei Miyashita
    • Organizer
      The 17th Awaji International Forum on Infection and Immunity
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Majority decisions by four or five members: social rules that can be formed and maintained in viral populations to exclude free riders.2018

    • Author(s)
      Shuhei Miyashita
    • Organizer
      Seminaire BGPI CIRAD
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comparative analysis of two-type of cell death developed in cucumber mosaic virus-inoculated leaves of Arabidopsis thaliana2018

    • Author(s)
      Ainan Tian, Shuhei Miyashita, Sugihiro Ando, Hieki Takahashi
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] R遺伝子による認識を免れる変異型CMV CPのアラニンスキャニングによる探索2018

    • Author(s)
      西條悠希, 安藤杉尋, 高橋英樹, 宮下脩平
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞間移行におけるCMVのMOIはR遺伝子による抵抗性誘導時に低下する2018

    • Author(s)
      鈴木万智, Sietske van Bentum, 安藤杉尋, 高橋英樹, 宮下脩平
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナへの無病徴感染に関わるキュウリモザイクウイルスのゲノムRNAと宿主因子の解析2018

    • Author(s)
      吉澤 峻, 宮下脩平, 安藤杉尋, 福原敏行, 高橋英樹
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] キュウリモザイクウイルスに無病_感染したシロイヌナズナにおけるRNA-seq解析2018

    • Author(s)
      高橋英樹, 田原 緑, 平山裕也, 宮下脩平, 安藤杉尋, 川野修一, 福原敏行
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Analysis of genomic region in cucumber mosaic virus RNA1 which determines ectopic induction of cell death in reassorted CMV-inoculated leaves of Arabidopsis thaliana2018

    • Author(s)
      Ainan Tian, Shuhei Miyashita, Sugihiro Ando, Hideki Takahashi
    • Organizer
      平成30年度日本植物病理学会東北部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物病原性Verticillium属菌から検出された複数のRNAウイルスのゲノム全塩基配列決定2018

    • Author(s)
      宮下脩平, Michael F Seidl, Jasper RL Depotter, 安藤杉尋, 高橋英樹, Bart PHJ Thomma
    • Organizer
      平成30年度日本植物病理学会東北部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] An endogenous pararetrovirus, petunia vein clearing virus, affects RNA interference in petunia2018

    • Author(s)
      Kazunori Kuriyama, Midori Tabara, Hideki Takahashi, Hiromitsu Moriyama and Toshiyuki Fukuhara
    • Organizer
      12th International Plant Molecular Biology Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-vitro biochemical characterization of a Dicer of Neurospora crassa, DCL2, in comparison with plant Dicers, DCL3 and DCL4 of Arabidopsis thaliana2018

    • Author(s)
      Tabara M, Suzuki N, Moriyama H, Fukuhara T.
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物に潜在感染しているウイルスの役割:ウイルスは植物の生存戦略に寄与できるか?2017

    • Author(s)
      高橋英樹
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ハクサンハタザオから単離したキュウリモザイクウイルスの性状解析2017

    • Author(s)
      高橋英樹・吉澤峻・宮下脩平・安藤杉尋
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] EMS変異誘発シロイヌナズナ系統におけるNB-LRRクラス抵抗性遺伝子RCY1プロモーター領域シトシンメチル化とCMV抵抗性レベル低下の相関2017

    • Author(s)
      佐藤有希代・宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒストン脱メチル化酵素変異体ldlにおけるキュウリモザイクウイルス抵抗性の解析2017

    • Author(s)
      小関彩恵子・宮下脩平・高橋英樹・安藤杉尋
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] キュウリモザイクウイルスの欠失変異パターンの解析2017

    • Author(s)
      本多宗一郎・安藤杉尋・高橋英樹・宮下脩
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2016 Annual Research Report
  • [Book] Antiviral Resistance in Plants: Methods and Protocols, In Methods in Molecular Biology (Eds. Kappei Kobayashi and Masamichi Nishiguchi)2019

    • Author(s)
      Yukiyo Sato and Hideki Takahashi
    • Total Pages
      278
    • Publisher
      Springer
    • ISBN
      9781493996346
    • Related Report
      2017 Annual Research Report
  • [Book] Cucumber Mosaic Virus (Eds. Peter Palukaitis and Fernando Garcia-Arenal)2018

    • Author(s)
      Chikara Masuta and Hideki Takahashi
    • Total Pages
      380
    • Publisher
      APS Press
    • ISBN
      9780890546093
    • Related Report
      2017 Annual Research Report
  • [Funded Workshop] WUR-TU Plant Science Workshop 2018(ワーゲニンゲン大学・東北大学 植物科学ワークショップ2018)2019

    • Related Report
      2017 Annual Research Report

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Published: 2016-07-04   Modified: 2021-12-27  

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