• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Production of rare bioactive natural products originating from uncultured bacteria

Planned Research

Project AreaCreation of Complex Functional Molecules by Rational Redesign of Biosynthetic Machineries
Project/Area Number 16H06448
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionHokkaido University

Principal Investigator

WAKIMOTO Toshiyuki  北海道大学, 薬学研究院, 教授 (70363900)

Project Period (FY) 2016-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥72,020,000 (Direct Cost: ¥55,400,000、Indirect Cost: ¥16,620,000)
Fiscal Year 2020: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2019: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2018: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2017: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Keywords海綿動物 / ポリケタイド / 細胞毒性物質 / 海綿 / Calyculin A / 環状ペプチド / 生合成 / 海綿共生微生物 / 共生微生物 / 異種発現 / calyculin A / 難培養微生物 / PKS / NRPS / 二次代謝産物 / 共生菌
Outline of Final Research Achievements

Marine sponges often contain potent cytotoxic compounds, which usually target molecules that are likely to be shared with the sponge itself. This fact in turn evokes the principle question of how marine sponges avoid self-toxicity. We previously identified an intriguing mechanism to prevent self-toxicity by the phosphorylation of the highly toxic calyculin A in the sponge holobiont, which is catalyzed by the phosphotransferase CalQ of a producer symbiont, “Candidatus Entotheonella” sp. However, the activating mechanism to dephosphorylate the stored phosphocalyculin A remains elusive. Here we show that the phosphatase specific to phosphocalyculin A is CalL, which is also encoded in the calyculin biosynthetic gene cluster. CalL represents a new clade and unprecedently coordinates the heteronuclear metals Cu and Zn. The CalL is localized in the periplasmic space of the sponge symbiont, where it is ready for the on-demand production of calyculin A in response to sponge tissue disruption.

Academic Significance and Societal Importance of the Research Achievements

海綿動物からは、これまでに多様な生物活性物質が単離報告されてきた。その中には、特異的かつ極めて低濃度で強力な活性を示す化合物が複数見出されており、それらは医薬品や生化学試薬の開発において重要な天然資源である。しかし海綿動物自体が稀少生物であることから、量的供給が難しく、その代謝物の医薬品開発を目指す上で大きな壁となっている。海綿を起源とする二次代謝産物を実験室下で異種生産した報告例はこれまでにほとんどないため、本研究は稀少な生物資源である海綿動物由来生物活性物質の生合成機構を解明する。本研究で得られる成果は、海綿及びその共生微生物に由来する医薬品資源の異種生産系の確立につながる。

Report

(6 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (71 results)

All 2021 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (35 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 34 results,  Open Access: 9 results,  Acknowledgement Compliant: 12 results) Presentation (25 results) (of which Int'l Joint Research: 14 results,  Invited: 25 results) Book (3 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Int'l Joint Research] スイス連邦工科大学チューリッヒ校(スイス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] スイス連邦工科大学チューリッ ヒ校(スイス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ETH Zurich(Switzerland)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization2021

    • Author(s)
      Matsuda Kenichi、Fujita Kei、Wakimoto Toshiyuki
    • Journal Title

      Journal of Industrial Microbiology and Biotechnology

      Volume: kuab023 Issue: 3-4

    • DOI

      10.1093/jimb/kuab023

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ascidian-associated photosymbionts originated from Manado, Indonesia: Secondary metabolite identification, bioactivity simulation, and biosynthetic insight2021

    • Author(s)
      Rumengan Inneke F.M.、 Roring Vera I.Y.、Haedar Jabal R.、 Siby Mayse S.、Luntungan Aldian H.、Kolondam Beivy J.、Uria Agustinus R.、Wakimoto Toshiyuki
    • Journal Title

      Symbiosis

      Volume: - Issue: 1 Pages: 71-82

    • DOI

      10.1007/s13199-021-00766-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 新規ペプチド環化酵素PBP-type TEの発見と機能解析2021

    • Author(s)
      松田研一、脇本敏幸
    • Journal Title

      ファインケミカル

      Volume: -

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Total Synthesis and Structural Revision of Kasumigamide, and Identification of a New Analogue2020

    • Author(s)
      Kuranaga Takefumi、Matsuda Kenichi、Takaoka Masachika、Tachikawa Chisato、Sano Ayae、Itoh Kosei、Enomoto Ayumu、Fujita Kei、Abe Ikuro、Wakimoto Toshiyuki
    • Journal Title

      ChemBioChem

      Volume: 21 Issue: 23 Pages: 3329-3332

    • DOI

      10.1002/cbic.202000409

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polyketides Biosynthesis in Marine Sponges of the Family Theonellidae2020

    • Author(s)
      Uria Agustinus R.、Wakimoto Toshiyuki
    • Journal Title

      Topics in Heterocyclic Chemistry

      Volume: - Pages: 1-25

    • DOI

      10.1007/7081_2020_48

    • ISBN
      9789811646362, 9789811646379
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterochiral coupling in non-ribosomal peptide macrolactamization2020

    • Author(s)
      Matsuda Kenichi、Zhai Rui、Mori Takahiro、Kobayashi Masakazu、Sano Ayae、Abe Ikuro、Wakimoto Toshiyuki
    • Journal Title

      Nature Catalysis

      Volume: 3 Issue: 6 Pages: 507-515

    • DOI

      10.1038/s41929-020-0456-7

    • NAID

      120006941707

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A New Cyclase Family Catalyzing Head-to-Tail Macrolactamization of Non-ribosomal Peptides2019

    • Author(s)
      Matsuda Kenichi、Kuranaga Takefumi、Wakimoto Toshiyuki
    • Journal Title

      Journal of Synthetic Organic Chemistry, Japan

      Volume: 77 Issue: 11 Pages: 1106-1115

    • DOI

      10.5059/yukigoseikyokaishi.77.1106

    • NAID

      130007742538

    • ISSN
      0037-9980, 1883-6526
    • Year and Date
      2019-11-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal Structures of Flavone <i>C</i>-Glycosides from Oolong Tea Leaves: Chafuroside A Dihydrate and Chafuroside B Monohydrate2019

    • Author(s)
      Iwao Yasunori、 Ishida Hitoshi、Kimura Shin-ichiro、Wakimoto Toshiyuki、Kondo Hiromu、Itai Shigeru、Noguchi Shuji
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 67 Issue: 9 Pages: 935-939

    • DOI

      10.1248/cpb.c19-00166

    • NAID

      130007699653

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2019-09-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Revised Structure of the Cyclic Octapeptide Surugamide A2019

    • Author(s)
      Matsuda Kenichi、Kuranaga Takefumi、Sano Ayae、Ninomiya Akihiro、Takada Kentaro、Wakimoto Toshiyuki
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 67 Issue: 5 Pages: 476-480

    • DOI

      10.1248/cpb.c19-00002

    • NAID

      130007641158

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2019-05-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Scrobiculosides A and B from the deep-sea sponge Pachastrella scrobiculosa2019

    • Author(s)
      Jomori Takahiro、Ise Yuji、Kohtsuka Hisanori、Matsuda Kenichi、Kuranaga Takefumi、Wakimoto Toshiyuki
    • Journal Title

      Journal of Natural Medicine

      Volume: 73 Issue: 4 Pages: 1058-1061

    • DOI

      10.1007/s11418-019-01315-6

    • NAID

      40021995195

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ペニシリン結合タンパク質によるペプチド環化2019

    • Author(s)
      松田 研一、倉永 健史、脇本 敏幸
    • Journal Title

      Farumashia

      Volume: 55 Issue: 7 Pages: 650-654

    • DOI

      10.14894/faruawpsj.55.7_650

    • NAID

      130007671605

    • ISSN
      0014-8601, 2189-7026
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] バクテリアが有する新たな非リボソームペプチド環化酵素2019

    • Author(s)
      松田研一、脇本敏幸
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 77 Pages: 306-307

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SurE is a trans-acting thioesterase cyclizing two distinct non-ribosomal peptides2019

    • Author(s)
      Matsuda, K., Kobayashi, M., Kuranaga, T., Takagda, K., Ikeda, H., Matsunaga, S., Wakimoto, T.,
    • Journal Title

      Org. Biomol. Chem.

      Volume: 17 Issue: 5 Pages: 1058-1061

    • DOI

      10.1039/c8ob02867b

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The revised structure of surugamide A2019

    • Author(s)
      Matsuda Kenichi、Kuranaga Takefumi、Ayae Sano, Akihiro Ninomiya、Takada Kentaros、Wakimoto Toshiyuki
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: -

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diastereoselective Total Synthesis and Structural Confirmation of Surugamide F2018

    • Author(s)
      Kuranaga Takefumi、Fukuba Atsuki、Ninomiya Akihiro、Takada Kentaro、Matsunaga Shigeki、Wakimoto Toshiyuki
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 66 Issue: 6 Pages: 637-641

    • DOI

      10.1248/cpb.c18-00072

    • NAID

      130007382323

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2018-06-01
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthetic Insights of Calyculin- and Misakinolide-Type Compounds in “ Candidatus Entotheonella sp.”2018

    • Author(s)
      Uria Agustinus R.、Piel Joern、Wakimoto Toshiyuki
    • Journal Title

      Methods in Enzymology

      Volume: 604 Pages: 287-330

    • DOI

      10.1016/bs.mie.2018.02.017

    • ISBN
      9780128139592
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Discovery of Unprecedented Hydrazine-Forming Machinery in Bacteria2018

    • Author(s)
      Matsuda Kenichi、Tomita Takeo、Shin-ya Kazuo、Wakimoto Toshiyuki、Kuzuyama Tomohisa、Nishiyama Makoto
    • Journal Title

      Journal of the American Chemical Society

      Volume: 140 Issue: 29 Pages: 9083-9086

    • DOI

      10.1021/jacs.8b05354

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Total Synthesis of the Nonribosomal Peptide Surugamide?B and Identification of a New Offloading Cyclase Family2018

    • Author(s)
      Kuranaga Takefumi、Matsuda Kenichi、Sano Ayae、Kobayashi Masakazu、Ninomiya Akihiro、Takada Kentaro、Matsunaga Shigeki、Wakimoto Toshiyuki
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 57 Issue: 30 Pages: 9447-9451

    • DOI

      10.1002/anie.201805541

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Palmitoyl lactic acid induces adipogenesis and a brown fat-like phenotype in 3T3-L1 preadipocytes2018

    • Author(s)
      Uno Yuka、Yamamoto Hirona、Shuto Takatsuki、Sato Yoshinori、Kuranaga Takefumi、Yazawa Kazunaga、Ono Yasuo、Wakimoto Toshiyuki
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids

      Volume: 1863 Issue: 7 Pages: 772

    • DOI

      10.1016/j.bbalip.2018.04.003

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diastereoselective total synthesis and structural confirmation of surugamide F2018

    • Author(s)
      Kuranaga Takefumi、Fukuba Atsuki、Ninomiya Akihiro、Takada Kentaro、Matsunaga Shigeki、Wakimoto Toshiyuki
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 66

    • NAID

      130007382323

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthetic insights of calyculin- and misakinolide-type compounds in “Candidatus Entotheonella sp.”2018

    • Author(s)
      Uria Agustinus、Piel Joern、Wakimoto Toshiyuki
    • Journal Title

      Methods in Enzymology

      Volume: 604

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Protein phosphatases 1 and 2A and their naturally occurring inhibitors: current topics in smooth muscle physiology and chemical biology2017

    • Author(s)
      Takai Akira、Eto Masumi、Hirano Katsuya、Takeya Kosuke、Wakimoto Toshiyuki、Watanabe Masaru
    • Journal Title

      J Physiol Sci

      Volume: 68 Issue: 1 Pages: 1-17

    • DOI

      10.1007/s12576-017-0556-6

    • NAID

      40021443577

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Total synthesis of theonellapeptolide Id2017

    • Author(s)
      Kuranaga, T., Enomoto, A., Tan, H., Fujita, K., Wakimoto, T.
    • Journal Title

      Org. Lett.

      Volume: 19 Issue: 6 Pages: 1366-1369

    • DOI

      10.1021/acs.orglett.7b00249

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Toward dark matter of natural products”2017

    • Author(s)
      Wakimoto, T.
    • Journal Title

      Chem. Rec.

      Volume: 17 Issue: 11 Pages: 1124-1134

    • DOI

      10.1002/tcr.201700009

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of pyridinium with three indole moieties as an antimicrobial agent2017

    • Author(s)
      Okada, M., Sugita, T., Wong, C. P., Wakimoto, T., Abe, I.
    • Journal Title

      J. Nat. Prod.,

      Volume: 80 Issue: 4 Pages: 1205-1209

    • DOI

      10.1021/acs.jnatprod.6b01152

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of giant modular PKSs provides insight into genetic mechanism for structural diversification of aminopolyol polyketides2017

    • Author(s)
      Zhang, L., Hashimoto, T., Qin, B., Hashimoto, J., Kozone, I., Kawahara, T., Okada, M., Awakawa, T., Ito, T., Asakawa, Y., Ueki, M., Takahashi, S., Osada, H., Wakimoto, T., Ikeda, H., Shin-ya, K., Abe, I.
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 56 Issue: 7 Pages: 1740-1745

    • DOI

      10.1002/anie.201611371

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] An unusual chimeric diterpene synthase from Emericella varicolor and its functional conversion into a sesterterpene synthase by domain swapping2016

    • Author(s)
      B. Qin, Y. Matsuda, T. Mori, M. Okada, Z. Quan, T. Mitsuhashi, T. Wakimoto, I. Abe
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 55 Issue: 5 Pages: 1658-1661

    • DOI

      10.1002/anie.201509263

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Metagenomic analysis of the sponge Discodermia reveals the production of the cyanobacterial natural product kasumigamide by 'Entotheonella'2016

    • Author(s)
      Nakashima, Y., Egami, Y., Kimura, M., Wakimoto, T., Abe, I.
    • Journal Title

      PLoS ONE

      Volume: 11 Issue: 10 Pages: e0164468-e0164468

    • DOI

      10.1371/journal.pone.0164468

    • NAID

      120005893293

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Sulfoureido lipopeptides from the marine sponge Discodermia kiiensis2016

    • Author(s)
      Tan, K. C., Wakimoto, T., Abe, I.
    • Journal Title

      J. Nat. Prod.

      Volume: 79 Issue: 9 Pages: 2418-2422

    • DOI

      10.1021/acs.jnatprod.6b00586

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structural diversification of lyngbyatoxin A by host-dependent heterologous expression of the tleABC biosynthetic gene cluster2016

    • Author(s)
      Zhang, L., Hoshino, S., Awakawa, T., Wakimoto, T., Abe, I.
    • Journal Title

      ChemBioChem

      Volume: 17 Issue: 15 Pages: 1407-1411

    • DOI

      10.1002/cbic.201600229

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Biosynthetic gene cluster for surugamide A encompasses an unrelated decapeptide, surugamide F2016

    • Author(s)
      Ninomiya, A., Katsuyama, Y., Kuranaga, T., Miyazaki, M., Nogi, Y., Okada, S., Wakimoto, T., Ohnishi, Y., Matsunaga, S., Takada, K.
    • Journal Title

      ChemBioChem

      Volume: 17 Issue: 18 Pages: 1709-1712

    • DOI

      10.1002/cbic.201600350

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Concise synthesis of anserine: efficient solvent tuning in asymmetric hydrogenation reaction2016

    • Author(s)
      M. Yamashita, K. Shimizu, Y. Koizumi, T. Wakimotoa, Y. Hamashima, T. Asakawa, M. Inai, T. Kan
    • Journal Title

      Synlet

      Volume: 27 Issue: 19 Pages: 2734-2736

    • DOI

      10.1055/s-0035-1562796

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Syntheses of methylated catechins and theaflavins using the Ns group to protect and deactivate the phenol2016

    • Author(s)
      T. Asakawa, Y. Kawabe, A. Yoshida, Y. Aihara, T. Manabe, Y. Hirose, A. Sakurada, M. Inai, Y. Hamashima, T. Furuta, T. Wakimoto, T. Kan
    • Journal Title

      J. Antibiot.

      Volume: 69 Issue: 4 Pages: 299-312

    • DOI

      10.1038/ja.2016.14

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calyculin: Nature’s way of making the sponge-derived cytotoxin2016

    • Author(s)
      T. Wakimoto, Y. Egami, I. Abe
    • Journal Title

      Nat. Prod. Rep.

      Volume: 未定 Issue: 6 Pages: 751-760

    • DOI

      10.1039/c5np00123d

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 海綿共生微生物による生物活性物質の生産2016

    • Author(s)
      江上蓉子, 脇本敏幸, 阿部郁朗
    • Journal Title

      バイオインダストリー

      Volume: 33 Pages: 11-17

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Discovery and functional analysis of a new peptide cyclase, PBP-type TE2021

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      1st GSD/GI-CoRE国際シンポジウム
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新規ペプチド環化酵素PBP-type TEの機能解析2021

    • Author(s)
      脇本敏幸
    • Organizer
      日本農芸化学会2021大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 海洋生物活性天然物の生合成に関する研究2021

    • Author(s)
      脇本敏幸
    • Organizer
      日本薬学会第141年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] A new off-loading cyclase in non-ribosomal peptide biosynthesis2020

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      3rd International Conference on Natural Product Discovery and Development in the Genomic Era
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] カリクリン生合成過程に内在する活性制御機構2020

    • Author(s)
      脇本敏幸
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 非リボソームペプチド新規環化酵素の発見2019

    • Author(s)
      脇本敏幸
    • Organizer
      第51回若手ペプチド夏の勉強会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 難培養微生物を起源とする希少医薬品資源の量産2019

    • Author(s)
      脇本敏幸
    • Organizer
      生物合成系の再設計による複雑骨格機能分子の革新的創成科学、第6回公開シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Chemical defense mechanism in a Japanese marine sponge, Discodermia calyx2019

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      Blue Pharmacy: from Sources to Clinical Reality
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] A new off-loading enzyme in non-ribosomal peptide biosynthesis2018

    • Author(s)
      Toshiyuki Wakimoto
    • Organizer
      2nd China-Japan Joint Symposium on Natural Product Biosynthesis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Marine Sponge-derived Metagenomes/uncultured Bacteria - a Rich Source of Novel Natural Product Biosynthetic Genes2018

    • Author(s)
      Toshiyuki Wakimoto
    • Organizer
      International Seminar on Natural Products Medicines 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Unusual Processes of PKS and NRPS Assembly Lines in Marine Bacteria2018

    • Author(s)
      Toshiyuki Wakimoto
    • Organizer
      1st German-Japanese Joint Symposium on the Biosynthesis of Natural Products
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生合成遺伝子から見出した新規天然物と難培養微生物2018

    • Author(s)
      脇本敏幸
    • Organizer
      新化学技術推進協会ライフサイエンス技術部会講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 難培養微生物を起源とする希少医薬品資源の量産2018

    • Author(s)
      脇本敏幸
    • Organizer
      生物合成系の再設計による複雑骨格機能分子の革新的創成科学、第2回公開シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] メタゲノムマイニングによる海綿由来新規天然物の探索2018

    • Author(s)
      脇本敏幸
    • Organizer
      日本薬学会第138会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Toward the dark matter of marine natural products2017

    • Author(s)
      Toshiyuki Wakimoto
    • Organizer
      International Symposium on Marine Natural Products
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2017-02-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] メタゲノムマイニングによる海綿由来新規天然物の探索2017

    • Author(s)
      脇本敏幸
    • Organizer
      理研シンポジウム、高磁場・高感度NMR利活用促進のための天然物分野における日中連携シンポジウム2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Marine sponge-derived metagenomes/uncultured bacteria - a rich source of novel natural product biosynthetic genes2017

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      South China Sea Institute of Oceanology, Chinese Academy of Sciences, China
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Marine sponge-derived metagenomes/uncultured bacteria - a rich source of novel natural product biosynthetic genes2017

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      Jinan University, College of Pharmacy, China
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Marine sponge-derived metagenomes/uncultured bacteria - a rich source of novel natural product biosynthetic genes2017

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      International Conference on Marine Biodiversity and Biotechnology, Jakarta, Indonesia
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Marine sponge-derived metagenomes/uncultured bacteria - a rich source of novel natural product biosynthetic genes2017

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      1st China-Japan Joint Symposium on Natural Product Biosynthesis, Shanghai, China
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Activated Chemical Defense of Japanese Marine Sponge Discodermia calyx2017

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      ISCE/APACE, Kyoto, Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Marine sponge-derived metagenomes/uncultured bacteria - a rich source of novel natural product biosynthetic genes2017

    • Author(s)
      Wakimoto Toshiyuki
    • Organizer
      9th US-Japan Seminar on Natural Product Biosynthesis, UCLA Lake Arrowhead Conference Center, California, USA
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A Bacterial Symbiont in Japanese Marine Sponges Produces Biologically Active Metabolites2016

    • Author(s)
      Toshiyuki Wakimoto
    • Organizer
      The 3rd Myanmar-Japan International Symposium
    • Place of Presentation
      Pathein, Myanmar
    • Year and Date
      2016-12-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 海綿動物由来生物活性物質の探索と生合成研究2016

    • Author(s)
      脇本敏幸
    • Organizer
      若手研究者のための有機化学札幌セミナー
    • Place of Presentation
      北海道大学理学部(北海道札幌市)
    • Year and Date
      2016-11-29
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Marine sponge-derived metagenomes/uncultured bacteria - a rich source of novel natural product biosynthetic genes2016

    • Author(s)
      Toshiyuki Wakimoto
    • Organizer
      Czech-Japan Joint Workshop
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] パートナー天然物化学(改訂第4版)2021

    • Author(s)
      海老塚 豊、森田 博史、阿部 郁朗、脇本 敏幸、他
    • Total Pages
      306
    • Publisher
      南江堂
    • ISBN
      9784524403325
    • Related Report
      2020 Annual Research Report
  • [Book] パートナー生薬学(改訂第4版)2021

    • Author(s)
      竹谷 孝一、木内 文之、小松 かつ子、脇本 敏幸、他
    • Total Pages
      458
    • Publisher
      南江堂
    • ISBN
      9784524403424
    • Related Report
      2020 Annual Research Report
  • [Book] 「天然物の化学ー魅力と展望ー」上村大輔編2016

    • Author(s)
      脇本敏幸
    • Publisher
      東京化学同人
    • Related Report
      2016 Annual Research Report
  • [Remarks] 北海道大学大学院薬学研究院天然物化学研究室

    • URL

      https://www.pharm.hokudai.ac.jp/tennen/index.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 北海道大学大学院薬学研究院天然物化学研究室

    • URL

      http://www.pharm.hokudai.ac.jp/tennen/index.html

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] 北海道大学プレスリリース

    • URL

      https://www.hokudai.ac.jp/news/180618_pr.pdf

    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] ペプチド類の大環状化酵素2019

    • Inventor(s)
      脇本敏幸、倉永健史、松田研一
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ペプチド類の大環状化酵素2018

    • Inventor(s)
      脇本敏幸、倉永健 史、松田研一
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-050797
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
    • Overseas

URL: 

Published: 2016-07-04   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi