Scrap& Build signal that dictates the synapse reformation

• Project Area: Dynamic regulation of brain function by Scrap & Build system
• Project/Area Number: 16H06457
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Suzuki Takashi 東京工業大学, 生命理工学院, 准教授 (60612760)
• Project Period (FY): 2016-06-30 – 2021-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥79,430,000 (Direct Cost: ¥61,100,000、Indirect Cost: ¥18,330,000); Fiscal Year 2020: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2019: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2018: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2017: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2016: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
• Keywords: ショウジョウバエ / 神経細胞 / 視神経系 / シナプス可塑性 / 軸索投射 / 成長円錐 / フィロポーディア / WNTシグナル / 視覚神経系 / 神経活動依存的 / 定数制御 / 神経科学 / 脳・神経 / 発生・分化 / 遺伝学 / 脳神経疾患 / 神経生物学 / 前シナプス活性帯 / 膜タンパク質 / 神経活性依存的 / 分子生物学 / 細胞生物学

Outline of Final Research Achievements

Neuronal Circuit undergoes filopodial scrap & build process during the developmental stages when axons search for their targets to form the circuit. It also undergoes synapse S&B process during adulthood to reform and modify the circuits to adapt to the changing environment, while maintaing its high-order bahaviours at the same time. In this studay, we have used drosohpila visual system as a model system to identify a number of S&B signals both for filopodia and synapses to reveal thier molecular mechanisms.

Academic Significance and Societal Importance of the Research Achievements

古くから神経が外界からの入力変化によってその受容の仕方が変化するということは、シナプス長期増強などを含めてよく研究されてきた。我々は、シナプスの経験依存的な変化には神経細胞間のコミュニケーションが重要な役割を担っているが、そのタンパク質をいくつか同定し、その作用機序を明らかにした。病理学的な側面として、このような成熟した後の神経回路におけるシナプス・スクラップ＆ビルドの不全が神経変性疾患などを引き起こす可能性が指摘されており、そのような病気の原因を明らかにするのに役に立つ可能性がある。

Research Products

• [Journal Article] Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light. (2017)
  • Author(s): Sugie A, Möhl C, Hakeda-Suzuki S, Matsui H, Suzuki T, Tavosanis G.
  • Journal Title: J Vis Exp Volume: -
  • DOI: 10.3791/55176
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] The genes required for synaptic plasticity in the Drosophila visual system. (2016)
  • Author(s): １８）Araki T., Kawamura H., Shimozono M., Sugie A., Hakeda-Suzuki S. and Suzuki, T.
  • Organizer: 第39回日本分子生物学会（MBSJ2016）
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] The source of Wg that is secreted in activity-dependent synaptic plasticity. (2016)
  • Author(s): Kawamura, H., Hakeda-Suzuki, S., Suzuki, T
  • Organizer: 第39回日本分子生物学会（MBSJ2016）
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] Disruption of synaptic structure induced by ALS causing gene TDP-43 in Drosophila visual system. (2016)
  • Author(s): ２１）Ohmae K, Takahashi F, Suzuki E, Hakeda-Suzuki S, Suzuki T.
  • Organizer: 第39回日本分子生物学会（MBSJ2016）
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] The genes required for synaptic plasticity in the Drosophila visual system. (2016)
  • Author(s): Araki T., Kawamura H., Shimozono M., Sugie A., Hakeda-Suzuki S. and Suzuki, T.
  • Organizer: 第12回日本ショウジョウバエ研究会 (JDRC12)
  • Place of Presentation: 立教大学
  • Year and Date: 2016-09-09
• [Presentation] Elucidating molecular mechanism of synaptic plasticity in living fruit fly using membrane potential proteins. (2016)
  • Author(s): Jovic D, Suzuki T, Kawamura H, Sugie A, Suzuki T.
  • Organizer: 第12回日本ショウジョウバエ研究会 (JDRC12),
  • Place of Presentation: 立教大学
  • Year and Date: 2016-09-09
• [Presentation] The source of Wg that is secreted in activity-dependent synaptic plasticity. (2016)
  • Author(s): Kawamura, H., Hakeda-Suzuki, S., Suzuki, T.
  • Organizer: 第12回日本ショウジョウバエ研究会 (JDRC12),
  • Place of Presentation: 立教大学
  • Year and Date: 2016-09-09
• [Presentation] Alteration in synaptic connection patterns in-vivo for different natural stimulations. (2016)
  • Author(s): Sathiyananthavel M, Suzuki T.
  • Organizer: 第12回日本ショウジョウバエ研究会 (JDRC12),
  • Place of Presentation: 立教大学
  • Year and Date: 2016-09-09
• [Presentation] Elucidation of the Molecular Mechanisms that Trigger Activity-dependent Reorganization of Presynaptic Active Zones in Fly Photoreceptors. (2016)
  • Author(s): Sugie A, Hakeda-Suzuki S, Suzuki E, Silies M, Shimozono M, Moehl C, Suzuki T*, and Tavosanis G*
  • Organizer: NeuroFly2016
  • Place of Presentation: Crete Island, Greece
  • Year and Date: 2016-09-02
  • Int'l Joint Research
• [Presentation] The genes required for synaptic plasticity in the Drosophila visual system. (2016)
  • Author(s): Araki, T., Shimozono, M., Sugie, A., Hakeda-Suzuki, S., and Suzuki, T.
  • Organizer: NeuroFly2016
  • Place of Presentation: Crete Island, Greece
  • Year and Date: 2016-09-02
  • Int'l Joint Research
• [Presentation] The source of Wg that is secreted in activity-dependent synaptic plasticity. (2016)
  • Author(s): Kawamura, H., Hakeda-Suzuki, S., Suzuki, T.
  • Organizer: NeuroFly2016
  • Place of Presentation: Crete Island, Greece
  • Year and Date: 2016-09-02
  • Int'l Joint Research
• [Presentation] The genes required for synaptic plasticity in the Drosophila visual system. (2016)
  • Author(s): Araki T., Kawamura H., Shimozono M., Sugie A., Hakeda-Suzuki S. and Suzuki, T.
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-07-20