受精における種間障壁のメカニズム解明とその打破

• Project Area: Determining the principles of the birth of new plant species: molecular elucidation of the lock-and-key systems in sexual reproduction
• Project/Area Number: 16H06465
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University
• Principal Investigator: 東山 哲也 名古屋大学, 理学研究科(WPI), 教授 (00313205)
• Co-Investigator(Kenkyū-buntansha): 金岡 雅浩 名古屋大学, 理学研究科, 講師 (10467277) ; Bode Jeffrey 名古屋大学, トランスフォーマティブ生命分子研究所, 客員教授 (90727900) ; 茂木 大和 (吉田大和) 東京大学, 大学院理学系研究科(理学部), 准教授 (80785444)
• Project Period (FY): 2016-06-30 – 2021-03-31
• Project Status: Granted (Fiscal Year 2020)
• Budget Amount: ¥128,440,000 (Direct Cost: ¥98,800,000、Indirect Cost: ¥29,640,000); Fiscal Year 2020: ¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000); Fiscal Year 2019: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000); Fiscal Year 2018: ¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000); Fiscal Year 2017: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000); Fiscal Year 2016: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
• Keywords: 受精 / 花粉管 / 種間障壁 / リガンド・レセプター / ペプチド

Outline of Annual Research Achievements

(1) 花粉管誘引におけるLURE およびその受容体複合体の作動実態解明　トレニアとシロイヌナズナのLUREにおけるドメインスワッピングにより見出した種認証に重要なアミノ酸の役割について明らかにするため、構造学的な検証を試みた。また、シロイヌナズナと近縁種の組み合わせをモデルとし、交雑授粉を行った場合の種間障壁の解析を進めた。LURE1およびPRK6の役割、助細胞由来のLURE-typeの他の誘引物質、長距離ガイダンスの役割について、解析を進めている。PRKファミリーの作動原理の解析も進めた。
(2) 精細胞放出における鍵と鍵穴分子の同定および動態解明　シロイヌナズナと、セイヨウミヤマハタザオをはじめとした近縁種を用いて、種認証過程のカルシウムイメージング系を確立した。種間交雑におけるカルシウムイメージングを進めるとともに、助細胞のカルシウムを上昇させる分子について、環状ペプチドをスクリーニングに着手した。種認証に関わる候補受容体子について、スワップ実験の準備を進めた。
(3) 種の壁の理解　配偶子の「融合」に加え「接着」の過程で働く分子の解析に向け、動物培養細胞を用いた実験系を拡張した接着におけるパートナー分子のスクリーニングに着手した。また、雌雄の細胞の稔性を向上させる生殖ホルモン分子を見出し、取りまとめを進めた。種間交雑の効率を向上させるためにも重要な分子と期待される。植物胚などを用いて、ゲノム倍加を促進する化合物の探索にも着手した。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

計画に基づき、受精における鍵と鍵穴分子の解析を進めている。高山班や渡辺班などと領域内共同研究により研究を進めている。重要な生殖ホルモンを見出し、その解析が進展している。

Strategy for Future Research Activity

(1) シロイヌナズナとセイヨウミヤマハタザオの組み合わせをモデルとし、高山班とも共同で、多段階の障壁を打破する。キメラLUREが受容体PRK6に作用する仕組みを、構造生物学により明らかにすることを目指す。また、渡辺班と共同で、花粉管の誘引ではなく伸長制御においてPRK6に作用するリガンドを探索し、1つの鍵穴が複数の鍵に対して作動する仕組みの解明を試みる。
(2) 受精能獲得においてはたらく鍵分子AMORとブラシノステロイドについて、有機合成化学や、関連化合物を用いた解析を進める。分子による受精能向上が種間障壁の打破に与える効果を明らかにする。また、受精における鍵と鍵穴、受精後のゲノム倍化を誘導する化合物の研究も進める。

Research Products

• [Int'l Joint Research] University of Porto(ポルトガル)
• [Int'l Joint Research] University of Milan(イタリア)
• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Int'l Joint Research] University of Hamburg(ドイツ)
• [Int'l Joint Research] 清華大学(中国)
• [Int'l Joint Research] UC Riverside(米国)
• [Int'l Joint Research] ハンブルグ大学/フライブルク大学(ドイツ)
• [Int'l Joint Research] グレゴールメンデル研究所(オーストリア)
• [Journal Article] Fertilization-Coupled Sperm Nuclear Fusion Is Required for Normal Endosperm Nuclear Proliferation (2020)
  • Author(s): Maruyama D., Higashiyama T., Endo T., Nishikawa S. I.
  • Journal Title: Plant and Cell Physiology Volume: 61 Pages: 29-40
  • DOI: 10.1093/pcp/pcz158
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of a novel method for the identification of fertilization defective mutants in Arabidopsis thaliana (2020)
  • Author(s): Liu Xiaoyan、Wu Xiaoyan、Adhikari Prakash Babu、Zhu Shaowei、Kinoshita Yoshihiro、Berger Frederic、Higashiyama Tetsuya、Kasahara Ryushiro D.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 521 Pages: 928-932
  • DOI: 10.1016/j.bbrc.2019.11.028
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development (2020)
  • Author(s): Motomura Kazuki、Arae Toshihiro、Araki-Uramoto Haruka、Suzuki Yuya、Takeuchi Hidenori、Suzuki Takamasa、Ichihashi Yasunori、Shibata Arisa、Shirasu Ken、Takeda Atsushi、Higashiyama Tetsuya、Chiba Yukako
  • Journal Title: Plant Cell Physiol. Volume: 61 Pages: 712-721
  • DOI: 10.1093/pcp/pcz235
  • Peer Reviewed / Open Access
• [Journal Article] Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes (2019)
  • Author(s): Kimata Y., Kato T., Higaki T., Kurihara D., Yamada T., Segami S., Morita M. T., Maeshima M., Hasezawa S., Higashiyama T., Tasaka M., Ueda M.
  • Journal Title: Proceedings of the National Academy of Sciences, U.S.A. Volume: 116 Pages: 2338-2343
  • DOI: 10.1073/pnas.1814160116
  • Peer Reviewed / Open Access
• [Journal Article] A homolog of the ALOG family controls corolla tube differentiation in Torenia fournieri (2019)
  • Author(s): Xiao Wei、Su Shihao、Higashiyama Tetsuya、Luo Da
  • Journal Title: Development Volume: 146
  • DOI: 10.1242/dev.177410
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of the Mitsucal computer system to identify causal mutation with a high-throughput sequencer (2018)
  • Author(s): Suzuki Takamasa、Kawai Tsutae、Takemura Shunsuke、Nishiwaki Marie、Suzuki Toshiya、Nakamura Kenzo、Ishiguro Sumie、Higashiyama Tetsuya
  • Journal Title: Plant Reproduction Volume: 31 Pages: 117-128
  • DOI: 10.1007/s00497-018-0331-8
  • Peer Reviewed
• [Journal Article] Quantitative assessment of chemotropism in pollen tubes using microslit channel filters (2018)
  • Author(s): Yanagisawa N and Higashiyama T
  • Journal Title: Biomicrofluidics Volume: 12 Pages: 024113-024113
  • DOI: 10.1063/1.5023718
  • Peer Reviewed / Open Access
• [Journal Article] MYB30 Links ROS Signaling, Root Cell Elongation and Plant Immune Responses. (2018)
  • Author(s): Kaho Mabuchi, Hiromasa Maki, Tomotaka Itaya, Takamasa Suzuki, Mika Nomoto, Satomi Sakaoka, Atsushi Morikami, Tetsuya Higashiyama, Yasuomi Tada, Wolfgang Busch, and Hironaka Tsukagoshi
  • Journal Title: Proceedings of the National Academy of Sciences Volume: -
  • DOI: 10.1073/pnas.1804233115
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ANTH domain-containing proteins are required for the pollen tube plasma membrane integrity via recycling ANXUR kinases (2018)
  • Author(s): Muro Keita、Matsuura-Tokita Kumi、Tsukamoto Ryoko、Kanaoka Masahiro M.、Ebine Kazuo、Higashiyama Tetsuya、Nakano Akihiko、Ueda Takashi
  • Journal Title: Commun. Biol. Volume: 1 Pages: 152-152
  • DOI: 10.1038/s42003-018-0158-8
  • Peer Reviewed / Open Access
• [Journal Article] The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants (2018)
  • Author(s): Takahashi T., Mori T., Ueda K., Yamada L., Nagahara S., Higashiyama T., Sawada H., Igawa T.
  • Journal Title: Development Volume: 145(23)
  • DOI: 10.1242/dev.170076
  • Peer Reviewed
• [Journal Article] Capacitation in Plant and Animal Fertilization (2018)
  • Author(s): Sankaranarayanan Subramanian、Higashiyama Tetsuya
  • Journal Title: Trends in Plant Science Volume: 23 Pages: 129-139
  • DOI: 10.1016/j.tplants.2017.10.006
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Special issue on plant reproduction research in Asia (2018)
  • Author(s): Sun Meng-Xiang、Yang Wei-Cai、Higashiyama Tetsuya
  • Journal Title: Plant Reproduction Volume: 31 Pages: 1-2
  • DOI: 10.1007/s00497-018-0330-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A pharmacological study of Arabidopsis cell fusion between the persistent synergid and endosperm. (2018)
  • Author(s): Motomura K, Kawashima T, Berger F, Kinoshita T, Higashiyama T, Maruyama D.
  • Journal Title: Journal of Cell Science Volume: 131 Pages: 1-6
  • DOI: 10.1242/jcs.204123
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Arabinogalactan proteins and their sugar chains: functions in plant reproduction, research methods, and biosynthesis (2018)
  • Author(s): Su Shihao、Higashiyama Tetsuya
  • Journal Title: Plant Reproduction Volume: 31 Pages: 67-75
  • DOI: 10.1007/s00497-018-0329-2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Pollen differentiation as well as pollen tube guidance and discharge are independent of the presence of gametes (2017)
  • Author(s): Glcokle Barbara、Urban Wojciech J.、Nagahara Shiori、Andersen Ellen D.、Higashiyama Tetsuya、Grini Paul E.、Schnittger Arp
  • Journal Title: Development Volume: 145
  • DOI: 10.1242/dev.152645
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote (2017)
  • Author(s): Ueda M, Aichinger E, Gong W, Groot E, Verstraeten I, Vu LD, De Smet I, Higashiyama T, Umeda M, Laux T
  • Journal Title: Genes Dev. Volume: 31 Pages: 617-627
  • DOI: 10.1101/gad.292409.116
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural basis for receptor recognition of pollen tube attraction peptides (2017)
  • Author(s): Zhang Xiaoxiao、Liu Weijia、Nagae Takuya T.、Takeuchi Hidenori、Zhang Heqiao、Han Zhifu、Higashiyama Tetsuya、Chai Jijie
  • Journal Title: Nature Communications Volume: 8 Pages: 1331-1331
  • DOI: 10.1038/s41467-017-01323-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Exocytosis-coordinated mechanisms for tip growth underlie pollen tube growth guidance (2017)
  • Author(s): Luo Nan、Yan An、Liu Gang、Guo Jingzhe、Rong Duoyan、Kanaoka Masahiro M.、Xiao Zhen、Xu Guanshui、Higashiyama Tetsuya、Cui Xinping、Yang Zhenbiao
  • Journal Title: Nature Communications Volume: 8 Pages: 1-10
  • DOI: 10.1038/s41467-017-01452-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Super-Photostable Phosphole-Based Dye for Multiple-Acquisition Stimulated Emission Depletion Imaging (2017)
  • Author(s): Wang C., Taki M., Sato Y., Fukazawa A., Higashiyama T., Yamaguchi S.
  • Journal Title: Journal of the American Chemical Society Volume: 139 Pages: 10374-10381
  • DOI: 10.1021/jacs.7b04418
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Fluorescent Labeling of the Cyst Nematode <i>Heterodera glycines</i> in Deep-Tissue Live Imaging (2017)
  • Author(s): Mina Ohtsu, Daisuke Kurihara, Yoshikatsu Sato, Takuya Suzaki, Masayoshi Kawaguchi, Daisuke Maruyama, Tetsuya Higashiyama
  • Journal Title: CYTOLOGIA Volume: 82 Issue: 3 Pages: 251-259
  • DOI: 10.1508/cytologia.82.251
  • NAID: 130006068235
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines. (2017)
  • Author(s): Ohtsu M., Sato Y., Kurihara D., Suzaki T., Kawaguchi M., Maruyama D. and Higashiyama T.
  • Journal Title: Protoplasma Volume: - Pages: 2107-2115
  • DOI: 10.1007/s00709-017-1105-0
  • NAID: 120006382262
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Capability of tip-growing plant cells to penetrate into extremely narrow gaps (2017)
  • Author(s): Naoki Yanagisawa, Nagisa Sugimoto, Hideyuki Arata, Tetsuya Higashiyama, Yoshikatsu Sato
  • Journal Title: Scientific Reports Volume: 7 Pages: 1403-1403
  • DOI: 10.1038/s41598-017-01610-w
  • Peer Reviewed / Open Access
• [Journal Article] Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells (2017)
  • Author(s): Taeko Sasaki, Yoshikatsu Sato, Tetsuya Higashiyama, Narie Sasaki
  • Journal Title: Scientific Reports Volume: 7 Pages: 11257-11257
  • DOI: 10.1038/s41598-017-10843-8
  • Peer Reviewed / Open Access
• [Journal Article] Pollen tube navigation can inspire microrobot design (2017)
  • Author(s): Higashiyama Tetsuya
  • Journal Title: Science Robotics Volume: 2
  • DOI: 10.1126/scirobotics.aao1891
  • Peer Reviewed
• [Journal Article] pKAMA-ITACHI Vectors for Highly Efficient CRISPR/Cas9-Mediated Gene Knockout in Arabidopsis thaliana (2017)
  • Author(s): Tsutsui H., Higashiyama T.
  • Journal Title: Plant Cell Physiol. Volume: 58 Pages: 46-56
  • DOI: 10.1093/pcp/pcw191
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structure-activity relation of AMOR sugar molecule that activates pollen-tubes for ovular guidance. (2017)
  • Author(s): Jiao J., Mizukami A.G., Sankaranarayanan S., Yamguchi J., Itami K., Higashiyama T.
  • Journal Title: Plant Physiology Volume: 173 Pages: 354-363
  • DOI: 10.1104/pp.16.01655
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Gametophytic pollen tube guidance: attractant peptides, gametic controls, and receptors (2017)
  • Author(s): Higashiyama T., Yang W.C.
  • Journal Title: Plant Physiol. Volume: 173 Pages: 112-121
  • DOI: 10.1104/pp.16.01571
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization. (2017)
  • Author(s): Kasahara R., Notaguchi M., Nagahara S., Suzuki T., Susaki D., Honma Y., Maruyama D., Higashiyama T.
  • Journal Title: Communicative & Integrative Biology Volume: 2 Pages: 1-7
  • DOI: 10.1126/sciadv.1600554
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote (2016)
  • Author(s): Kimata Y., Higaki T., Kawashima T., Kurihara D., Sato Y., Yamada T., Hasezawa S., Berger F., Higashiyama T., Ueda M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 113 Pages: 14157-14162
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Key signalling molecules in pollen tube guidance (2020)
  • Author(s): Higashiyama T.
  • Organizer: Symposium Down Under: Mechanisms controlling plant reproduction
  • Int'l Joint Research / Invited
• [Presentation] Live-Cell Analysis of Multi-Step Signaling in Pollen Tube Guidance (2019)
  • Author(s): Higashiyama T.
  • Organizer: The 11th Tri-National Arabidopsis Meeting (TNAM 2019)
  • Int'l Joint Research / Invited
• [Presentation] Live-cell analysis of plant reproduction (2019)
  • Author(s): Higashiyama T.
  • Organizer: EMBO practical course “Functional Imaging of Plants”
  • Int'l Joint Research / Invited
• [Presentation] Key molecules of sexual reproduction identified by live-cell and synthetic-chemistry approaches (2019)
  • Author(s): Higashiyama T.
  • Organizer: 22nd Plant Biology Symposium: Plant Cell Dynamics
  • Int'l Joint Research / Invited
• [Presentation] Receptor-like kinases in plant reproduction (2019)
  • Author(s): Higashiyama T.
  • Organizer: International Symposium on Plant Receptor Kinases and Cell Signaling 2019 (PRKCS2019)
  • Int'l Joint Research / Invited
• [Presentation] Evolution of Sexual Plant Reproduction and Its Key Molecules (2019)
  • Author(s): Higashiyama T.
  • Organizer: 5th International Volvox Conference (Volvox 2019)
  • Int'l Joint Research / Invited
• [Presentation] Key signalling molecules in plant reproduction: Re-discovery of a reproductive hormone (2019)
  • Author(s): Higashiyama T.
  • Organizer: Woolhouse Lecture of John Innes Seminar
  • Int'l Joint Research / Invited
• [Presentation] Live-Cell Analysis of Molecules Involved in Pollen Tube Guidance (2018)
  • Author(s): Higashiyama T.
  • Organizer: The 25th International Congress on Sexual Plant Reproduction
  • Int'l Joint Research / Invited
• [Presentation] Cruising inside flowers: from cellular dynamics to key signaling molecules (2018)
  • Author(s): Higashiyama T.
  • Organizer: International Conference on Arabidopsis Research ICAR2018
  • Int'l Joint Research / Invited
• [Presentation] Visualization and manipulation of genomes in sexual reproduction (2018)
  • Author(s): Higashiyama T.
  • Organizer: Plant Genomes in a Changing Environment
  • Int'l Joint Research / Invited
• [Presentation] Dynamics and key signaling molecules of pollen tube guidance (2018)
  • Author(s): Higashiyama T.
  • Organizer: HBMC (Horticulture Biology and Metabolomics Center) Symposium in Fujian Agriculture and Forestry University
  • Int'l Joint Research / Invited
• [Presentation] Dissection and Maipulation of Species-Barrier Molecules (2018)
  • Author(s): Higashiyama T.
  • Organizer: The 41st Annual Meeting of the Molecular Biology Society of Japan, Symposium “Cutting edge biology led by plant models”
  • Int'l Joint Research / Invited
• [Presentation] Live-Cell Analyses of Key Signaling Molecules in Pollen Tube Guidance (2017)
  • Author(s): Higashiyama T.
  • Organizer: Taiwan Japan Plant Biology 2017
  • Int'l Joint Research / Invited
• [Presentation] Live-cell analysis of pollen tube guidance identifies key signaling molecules: Cruising inside the flower (2017)
  • Author(s): Higashiyama T.
  • Organizer: Canadian Microscopy and Cytometry Symposium
  • Int'l Joint Research / Invited
• [Presentation] Pollen tube guidance inside flowers (2017)
  • Author(s): Higashiyama T.
  • Organizer: Workshop on Molecular Mechanisms Controlling Flower Development
  • Int'l Joint Research / Invited
• [Presentation] Cell-to-Cell Signaling Molecules and Dynamics of Pollen Tube Guidance (2017)
  • Author(s): Higashiyama T.
  • Organizer: 50th Annual Meeting of the Japanese Society of Developmental Biologists, Symposium “Fertilization”
  • Int'l Joint Research / Invited
• [Presentation] Live-Cell Imaging of Pollen Tube Guidance for Identification of Key Molecules (2017)
  • Author(s): Higashiyama T.
  • Organizer: The 18th Congess of the International Society of Developmental Biologists
  • Int'l Joint Research / Invited
• [Presentation] POLLEN RECEPTOR-LIKE KINASE family and RAC/ROP GTPase signaling in directional tip growth of the pollen tube (2017)
  • Author(s): Higashiyama T.
  • Organizer: IBC2017 -XIX International Botanical Congress, symposium “Signaling by RAC/ROP GTPases and G-proteins”
  • Int'l Joint Research / Invited
• [Presentation] How do plant female gametes prevent and allow polyspermy in double fertilization? (2017)
  • Author(s): Higashiyama T.
  • Organizer: IBC2017 -XIX International Botanical Congress, symposium “Germline development and fertilization mechanisms”
  • Int'l Joint Research / Invited
• [Presentation] Live-Cell Analyses of Key Signaling Molecules in Pollen Tube Guidance (2017)
  • Author(s): Higashiyama T.
  • Organizer: Keystone Symposium on “Plant Signaling: Molecular Pathways and Network Integration”
  • Int'l Joint Research / Invited
• [Presentation] Live-Cell Analysis of Pollen Tube Guidance to Identify Key Signaling Molecules (2017)
  • Author(s): Higashiyama T.
  • Organizer: Seminar in The Plant Gene Expression Center (PGEC), University of California, Berkeley
  • Place of Presentation: バークレー
  • Int'l Joint Research / Invited
• [Presentation] Live-Cell Analysis of Pollen Tube Guidance Reveals Key Signaling Molecules (2017)
  • Author(s): Higashiyama T.
  • Organizer: Seminar in Carnegie Institution for Science
  • Place of Presentation: パロアルト
  • Int'l Joint Research / Invited
• [Presentation] Signaling in Pollen Tube Guidance (2016)
  • Author(s): Higashiyama T.
  • Organizer: CSH Asia Conference on Latest Advances in Plant Development & Environmental Response
  • Place of Presentation: 淡路島
  • Year and Date: 2016-11-29
  • Int'l Joint Research / Invited
• [Presentation] 種の壁を超える分子 (2016)
  • Author(s): 東山哲也
  • Organizer: 第39回日本分子生物学会年会、シンポジウム「最先端の化学と生物学のミックス」
  • Place of Presentation: 横浜
  • Invited
• [Book] 糖鎖生物学 (2020)
  • Author(s): 北島 健、佐藤 ちひろ、門松 健治、加藤 晃一
  • Total Pages: 306
  • Publisher: 名古屋大学出版会
  • ISBN: 9784815809812
• [Remarks] トランスフォーマティブ生命分子研究所
  • URL: http://www.itbm.nagoya-u.ac.jp/index-ja.php
• [Remarks] 植物新種誕生の原理
  • URL: http://www.ige.tohoku.ac.jp/prg/plant/
• [Remarks] 名古屋大学トランスフォーマティブ生命分子研究所
  • URL: http://www.itbm.nagoya-u.ac.jp/index-ja.php
• [Patent(Industrial Property Rights)] 花粉管成長調節剤 (2019)
  • Inventor(s): 東山哲也，時田公 美，伊丹健一郎，上 田彩果，他3名
  • Industrial Property Rights Holder: 東山哲也，時田公 美，伊丹健一郎，上 田彩果，他3名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] 水溶性ワープドナノグラフェン化合物及びその用途 (2017)
  • Inventor(s): 伊丹健一郎，瀬川泰知， 林興安，東山哲也 他3名
  • Industrial Property Rights Holder: 伊丹健一郎，瀬川泰知， 林興安，東山哲也 他3名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] Casタンパク質発現カセット (2016)
  • Inventor(s): 東山哲也、筒井大貴
  • Industrial Property Rights Holder: 東山哲也、筒井大貴
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-09-02