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Visualization and photomanipulation of migrating neuronal cells and surrounded field during the formation of brain tissue

Planned Research

Project AreaInterplay of developmental clock and extracellular environment in brain formation
Project/Area Number 16H06487
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

松田 知己  大阪大学, 産業科学研究所, 准教授 (50419206)

Project Period (FY) 2016-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥84,760,000 (Direct Cost: ¥65,200,000、Indirect Cost: ¥19,560,000)
Fiscal Year 2020: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2019: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2018: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2017: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2016: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Keywordsライブイメージング / 蛍光タンパク質 / 化学発光 / 細胞動態 / 生物発光
Outline of Annual Research Achievements

本研究では、場(細胞外環境)と神経細胞の連携という新たな視点で脳発生時間の制御機構を解明することを目的とした本新学術領域研究の発展に不可欠な、神経細胞のダイナミックな移動に伴う分子・細胞機能の変化を捉えるライブイメージングツールの開発を行なった。
研究グループの着想に基づいて、化学発光Mg2+センサーを開発した。本センサーは、中心体タンパク質セントリン3のMg2+センシングドメインを黄色蛍光タンパク質Venusの円順列変異体と化学発光タンパク質NanoLucで挟んだドメイン構成で、Mg2+濃度上昇に伴って長波長にスペクトルシフトを起こす。本研究成果はSensors誌にて発表した。
領域研究者同士の情報交換から着想を得た開発として、計画班仲嶋班と共同でN-カドヘリンを介した細胞間相互作用を可視化するセンサーの開発を進めてきた。二量体形成により蛍光発光するβバレル型蛍光タンパク質のペア(ddGFP)を用いた本センサーのシグナル/バックグラウンド比は、蛍光タンパク質間のFRETを利用した既存のセンサーに比べて70倍程度大きいことを示した。また、計画班永樂班と共同で、多細胞からなるオルガノイド内での2光子顕微鏡観察下で、細胞間N-カドヘリン相互作用を可視化できることを示した。以上の研究成果は論文投稿済みで、査読コメントへの対応を行なっている段階にある。
N-カドヘリン相互作用センサー開発の経験を生かして、神経回路構築・シナプス形成に重要な役割を果たすプロトカドヘリンについて、同一神経細胞由来の神経突起間での相互作用の可視化を目指したFRETセンサーの開発を行い、Scientific Reports誌にて発表した。
さらに、メカニカルストレス応答分子YAPのリン酸化状態を可視化するプローブのプロトタイプを作製し、計画班影山班、公募班佐々木班に評価を依頼している。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Report

(5 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report

Research Products

(49 results)

All 2022 2021 2020 2019 2018 2017 2016 Other

All Journal Article (16 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 14 results,  Open Access: 10 results) Presentation (24 results) (of which Int'l Joint Research: 7 results,  Invited: 6 results) Book (4 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin2021

    • Author(s)
      Kanadome Takashi、Hoshino Natsumi、Nagai Takeharu、Matsuda Tomoki、Yagi Takeshi
    • Journal Title

      Scientific Reports

      Volume: 11 Pages: 22237-22237

    • DOI

      10.1038/s41598-021-01481-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water2020

    • Author(s)
      Hossain Md Nadim、Ishida Ryuichi、Hattori Mitsuru、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      Sensors

      Volume: 20 Pages: 3164-3164

    • DOI

      10.3390/s20113164

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 多色の光増感蛍光タンパク質を用いた複数機能の個別破壊2020

    • Author(s)
      松田知己、永井健治
    • Journal Title

      アグリバイオ

      Volume: 4 Pages: 61-65

    • NAID

      40022457168

    • Related Report
      2020 Annual Research Report
  • [Journal Article] 光増感蛍光タンパク質を用いた多色機能破壊2020

    • Author(s)
      松田 知己、永井 健治
    • Journal Title

      月刊 細胞

      Volume: 52 Pages: 93-96

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Hierarchical Development of Motile Polarity in Durotactic Cells Just Crossing an Elasticity Boundary2020

    • Author(s)
      Thasaneeya Kuboki, Hiroyuki Ebata, Tomoki Matsuda, Yoshiyuki Arai, Takeharu Nagai, Satoru Kidoaki
    • Journal Title

      Cell Struct. Funct.

      Volume: 45 Issue: 1 Pages: 33-43

    • DOI

      10.1247/csf.19040

    • NAID

      130007801487

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging2019

    • Author(s)
      Ryosuke Oketani, Haruka Suda, Kumiko Uegaki, Toshiki Kubo, Tomoki Matsuda, Masahito Yamanaka, Yoshiyuki Arai, Nicholas I. Smith, Takeharu Nagai, Katsumasa Fujita
    • Journal Title

      Journal of Biomedical Optics

      Volume: 25 Pages: 014502-014502

    • DOI

      10.1117/1.jbo.25.1.014502

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions2019

    • Author(s)
      Shinoda Hajime、Lu Kai、Nakashima Ryosuke、Wazawa Tetsuichi、Noguchi Kosuke、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      Cell Chemical Biology

      Volume: 26

    • DOI

      10.1016/j.chembiol.2019.07.012

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetically encoded fluorescence/bioluminescence bimodal indicators for Ca2+ imaging2019

    • Author(s)
      Israt Farhana, Md Nadim Hossain, Kazushi Suzuki,Tomoki Matsuda, Takeharu Nagai
    • Journal Title

      ACS Sensors

      Volume: 4 Pages: 1825-1834

    • DOI

      10.1021/acssensors.9b00531

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bioluminescent Low-Affinity Ca2+ Indicator for ER with Multicolor Calcium Imaging in Single Living Cells2018

    • Author(s)
      Hossain Md Nadim、Suzuki Kazushi、Iwano Megumi、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      ACS Chem. Biol.

      Volume: 13 Pages: 1862-1871

    • DOI

      10.1021/acschembio.7b01014

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation2018

    • Author(s)
      Riani Yemima Dani、Matsuda Tomoki、Takemoto Kiwamu、Nagai Takeharu
    • Journal Title

      BMC Biology

      Volume: 16 Pages: 1-12

    • DOI

      10.1186/s12915-018-0514-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease2018

    • Author(s)
      Hara-Kuge Sayuri、Nishihara Tomonobu、Matsuda Tomoki、Kitazono Tomohiro、Teramoto Takayuki、Nagai Takeharu、Ishihara Takeshi
    • Journal Title

      PLOS ONE

      Volume: 13

    • DOI

      10.1371/journal.pone.0194707

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca2+ microscopy2018

    • Author(s)
      Suzuki Kazushi、Onishi Takahito、Nakada Chieko、Takei Shunsuke、Daniels Matthew J.、Nakano Masahiro、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      BMC Research Notes

      Volume: 11 Pages: 313-313

    • DOI

      10.1186/s13104-018-3421-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging2018

    • Author(s)
      Arai Yoshiyuki、Takauchi Hiroki、Ogami Yuhei、Fujiwara Satsuki、Nakano Masahiro、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      ACS Chemical Biology

      Volume: 13 Pages: 1938-1943

    • DOI

      10.1021/acschembio.8b00200

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acid-Tolerant Monomeric GFP from Olindias formosa2018

    • Author(s)
      Shinoda Hajime、Ma Yuanqing、Nakashima Ryosuke、Sakurai Keisuke、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      Cell Chemical Biology

      Volume: 25

    • DOI

      10.1016/j.chembiol.2017.12.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation2018

    • Author(s)
      Maeshima Kazuhiro、Matsuda Tomoki、Shindo Yutaka、Imamura Hiromi、Tamura Sachiko、Imai Ryosuke、Kawakami Syoji、Nagashima Ryosuke、Soga Tomoyoshi、Noji Hiroyuki、Oka Kotaro、Nagai Takeharu
    • Journal Title

      Current Biology

      Volume: 28 Pages: 444-451

    • DOI

      10.1016/j.cub.2017.12.035

    • NAID

      120006380520

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methods for monitoring signaling molecules in cellular compartments2017

    • Author(s)
      Agetsuma Masakazu、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      Cell Calcium

      Volume: 64 Pages: 12-19

    • DOI

      10.1016/j.ceca.2016.12.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Development of fluorescent indicators for visualization of N-cadherin interaction across cells2022

    • Author(s)
      Tomoki Matsuda
    • Organizer
      The International Symposium on Development and Plasticity of Neural Systems
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ライブイメージング・操作のための高機能蛍光タンパク質の開発2021

    • Author(s)
      松田 知己、永井 健治
    • Organizer
      レーザー学会 学術講演会 第41回年次大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 蛍光タンパク質の基本的性質と多角的な利用 / X-Light V2を用いたSRRF超解像イメージング2021

    • Author(s)
      松田知己
    • Organizer
      Imaging Bootcamp, 北海道大学大学院医学研究院 細胞生理学教室 主催、文部科学省科学研究費助成事業学術変革領域研究(A)「物質共生」共済
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Acid-resistant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging in Acidic Environments2019

    • Author(s)
      Hajime Shinoda, Ryosuke Nakashima, Tetsuichi Wazawa, Kosuke Noguchi, Tomoki Matsuda, Takeharu Nagai
    • Organizer
      Focus on Microscope, London, United Kigdom
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光刺激によりタンパク質機能阻害・細胞死を誘導する単量体光増感緑色蛍光タンパク質2019

    • Author(s)
      Yemima Dani Riani, 松田 知己, 竹本 研, 永井 健治
    • Organizer
      第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会, 神戸国際会議場
    • Related Report
      2019 Annual Research Report
  • [Presentation] Monomeric green fluorescent protein based photosensitizer for photo-inducible protein inactivation and cell death2019

    • Author(s)
      Tomoki Matsuda, Yemima Dani Riani, Kiwamu Takemoto, Takeharu Nagai
    • Organizer
      第57回日本生物物理学会年会, 宮崎県・シーガイアコンベンションセンター
    • Related Report
      2019 Annual Research Report
  • [Presentation] Acid-tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging in Acidic Conditions2019

    • Author(s)
      Hajime Shinoda, Kai Lu, Ryosuke Nakashima, Tetsuichi Wazawa, Kosuke Noguchi, Tomoki Matsuda, Takaharu Nagai
    • Organizer
      第57回日本生物物理学会年会, 宮崎県・シーガイアコンベンションセンター
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fluorescent Ca2+ indicators for multiplexed super-resolution imaging at nanoscopic cellular domain2019

    • Author(s)
      Kai Lu, Tomoki Matsuda, Tetsuichi Wazawa, Takeharu Nagai
    • Organizer
      第57回日本生物物理学会年会, 宮崎県・シーガイアコンベンションセンター
    • Related Report
      2019 Annual Research Report
  • [Presentation] Photosensitizing green fluorescent protein for photo-inducible protein inactivation and cell death Presenter2019

    • Author(s)
      Tomoki Matsuda, Yemima Dani Riani, Kiwamu Takemoto, Takeharu Nagai
    • Organizer
      第42回日本分子生物学会年会, 福岡国際会議場
    • Related Report
      2019 Annual Research Report
  • [Presentation] A bimodal bioluminescent Ca2+ indicator toward spatiotemporally-scalable imaging2019

    • Author(s)
      Tomoki Matsuda, Israt Farhana, Kazhshi Suzuki and Takeharu Nagai
    • Organizer
      SPIE. PHOTONICS WEST BIOS, The Moscone Center San Francisco, United States
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Bimodal Ca2+ indicator toward spatiotemporally-scalable imaging2018

    • Author(s)
      Israt Farhana, Kazushi Suzuki, Tomoki Matsuda, Takeharu Nagai
    • Organizer
      生理学研究所研究会, 生理学研究所
    • Related Report
      2018 Annual Research Report
  • [Presentation] Fluorescent and Bioluminescent Protein Based Probes for Visualization of Biological events2018

    • Author(s)
      Tomoki Matsuda
    • Organizer
      24th iCeMS International Symposium, iCeMS Kyoto University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 蛍光タンパク質とハサミは使いよう2018

    • Author(s)
      松田 知己
    • Organizer
      日本化学会生体機能関連化学部会若手の会, ANA ホリデイ・イン リゾート 宮崎
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ライブイメージングツール開発のための蛍光・生物発光蛋白質工学2018

    • Author(s)
      松田 知己
    • Organizer
      第18回 日本蛋白質科学会年会, 朱鷺メッセ
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光スイッチング Ca2+指示薬 -超解像機能イメージングを目指して-2017

    • Author(s)
      松田 知己、藤原 沙都姫、永井 健治
    • Organizer
      生理学研究所研究会「シグナル動態の可視化と操作に基づく多階層機能解析の新展開」, 生理学研究所
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酸性オルガネラ内機能イメージングのための、耐酸性GFP の開発2017

    • Author(s)
      篠田 肇、Yuanqing Ma、中島 良介、櫻井 啓介、松田 知己、永井 健治
    • Organizer
      生理学研究所研究会「シグナル動態の可視化と操作に基づく多階層機能解析の新展開」, 生理学研究所
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of chemiluminescent low affinity Ca2+ indicators applicable to analyzing Ca2+ dynamics in endoplasmic reticulum2017

    • Author(s)
      Nadim H、鈴木 和志、岩野 恵、松田 知己、永井 健治
    • Organizer
      生理学研究所研究会「シグナル動態の可視化と操作に基づく多階層機能解析の新展開」, 生理学研究所
    • Related Report
      2017 Annual Research Report
  • [Presentation] ハナガサクラゲ由来の耐酸性・単量体型GFP2017

    • Author(s)
      篠田 肇、Ma Yuanqing、中島 良介、櫻井 啓介、松田 知己、永井 健治
    • Organizer
      第55回日本生物物理学会年会, 熊本大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] Multicolor Bioluminescent Calcium Imaging Across Three Orders of [Ca2+] Magnitude in Single Living Cells2017

    • Author(s)
      Nadim H, Suzuki K, Iwano M, Matsuda T, Nagai T.
    • Organizer
      20th International Symposium on Calcium Binding Proteins and Calcium Function in Health and Disease, Awaji Yumebutai
    • Related Report
      2017 Annual Research Report
  • [Presentation] Fluorescent/Bioluminescent Protein-Based Ca2+ Probes and Photo Manipulation for Imaging of Physiological Functions2017

    • Author(s)
      Matsuda T, Nagai T.
    • Organizer
      20th International Symposium on Calcium Binding Proteins and Calcium Function in Health and Disease, Awaji Yumebutai
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 生理機能イメージングのための蛍光・発光タンパク質を用いたCa2+プローブと光操作2017

    • Author(s)
      松田 知己、永井 健治
    • Organizer
      2017年度生命科学系学会合同年次大会, 神戸ポートアイランド
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of Chemiluminescent Ca2+ Indicators with Expanded Dynamic Range2016

    • Author(s)
      Mai Ashitani, Kazushi Suzuki, Tomoki Matsuda and Takeharu Nagai
    • Organizer
      The 20th ISIR International Symposium “Molecular Technology Frontiers towards IoT World”
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Acid resistant monomeric GFP derived from Olindias formosa2016

    • Author(s)
      Hajime Shinoda , Yuanqing Ma, Ryosuke Nakashima, Keisuke Sakurai, Akihito Yamaguchi, Tomoki Matsuda, Takeharu Nagai
    • Organizer
      The 20th ISIR International Symposium “Molecular Technology Frontiers towards IoT World”
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Bioluminescent Low Affinity Ca2+ Indicators Applicable to Analysis of Ca2+ Dynamics in Endoplasmic Reticulum2016

    • Author(s)
      Nadim Hossain, Kazushi Suzuki, Megumi Iwano, Tomoki Matsuda and Takeharu Nagai
    • Organizer
      The 20th ISIR International Symposium “Molecular Technology Frontiers towards IoT World”
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] 先端の分析法 第2版 / 永井 健治, 松田 知己, 鈴木 和志, 篠田 肇, タンパク質プローブ, pp.317-3232022

    • Author(s)
      澤田 嗣郎、小澤 岳昌、北森 武彦、中村 洋(東京理科大学名誉教授)、藤浪 眞紀、宮村 一夫、石丸 洋一郎、浦野 泰照、加地 範匡、坂 真智子、鈴木 茂、瀬藤 光利、宗林 由樹、馬場 嘉信、船津 公人、本田 暁紀、末永 智一、宮野 博、本山 晃
    • Total Pages
      1072
    • Publisher
      エヌ・ティー・エス
    • ISBN
      9784860437374
    • Related Report
      2020 Annual Research Report
  • [Book] Live Cell Imaging / Mitsuru Hattori, Tomoki MatsudaTakeharu Nagai, Method for Detecting Emission Spectral Change of Bioluminescent Ratiometric Indicators by a Smartphone, Chapter 25, pp295~3042021

    • Author(s)
      Editor: Sung-Bae Kim
    • Total Pages
      447
    • Publisher
      Humana, New York, NY
    • ISBN
      9781071612576
    • Related Report
      2020 Annual Research Report
  • [Book] Advances in Experimental Medicine and Biology, Optogenetics / Yemima Dani Riani, Tomoki Matsuda, Takeharu Nagai, Genetically Encoded Photosensitizer for Destruction of Protein or Cell Function, pp 265~2792021

    • Author(s)
      Editors: Hiromu Yawo, Hideki Kandori, Amane Koizumi, Ryoichiro Kageyama
    • Total Pages
      663
    • Publisher
      Springer, Singapore
    • ISBN
      9789811587627
    • Related Report
      2020 Annual Research Report
  • [Book] Methods in Molecular Biology, Multiplexed Imaging / Kazushi Suzuki, Md Nadim Hossain, Tomoki Matsuda, Takeharu Nagai, Multicolor Bioluminescence Imaging of Subcellular Structures and Multicolor Calcium Imaging in Single Living Cells, pp 229~2372021

    • Author(s)
      Editor: Eli Zamir
    • Total Pages
      343
    • Publisher
      Humana, New York, NY
    • ISBN
      9781071615928
    • Related Report
      2020 Annual Research Report
  • [Remarks] 耐酸性・単量体型緑色蛍光タンパク質Gamillusの開発

    • URL

      https://www.sanken.osaka-u.ac.jp/labs/bse/fluorescentprotein.html

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] 発光・蛍光タンパク質プローブの開発 耐酸性・単量体型緑色蛍光タンパク質Gamillusの開発

    • URL

      https://www.sanken.osaka-u.ac.jp/labs/bse/research_101205_1.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 発光・蛍光タンパク質プローブの開発 耐酸性・単量体型緑色蛍光タンパク質Gamillusの開発

    • URL

      http://www.sanken.osaka-u.ac.jp/labs/bse/research_101205_1.html

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 蛍光タンパク質2018

    • Inventor(s)
      永井健治、篠田肇、松田知己、マ ユアンキン
    • Industrial Property Rights Holder
      永井健治、篠田肇、松田知己、マ ユアンキン
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Acquisition Date
      2021
    • Related Report
      2020 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 蛍光タンパク質2018

    • Inventor(s)
      永井健治、篠田肇、松田知己、マ ユアンキン
    • Industrial Property Rights Holder
      永井健治、篠田肇、松田知己、マ ユアンキン
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Acquisition Date
      2020
    • Related Report
      2020 Annual Research Report

URL: 

Published: 2016-07-04   Modified: 2022-12-28  

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