Cortical local circuits for multi-area interconnectivity and parallel processing

• Project Area: Brain information dynamics underlying multi-area interconnectivity and parallel processing
• Project/Area Number: 17H06311
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Tamagawa University (2019-2021); National Institute for Physiological Sciences (2017-2018)
• Principal Investigator: 川口 泰雄 玉川大学, 脳科学研究所, 研究員 (40169694)
• Co-Investigator(Kenkyū-buntansha): 大塚 岳 生理学研究所, 脳機能計測・支援センター, 助教 (10390692) ; 高田 美絵子 (森島美絵子) 東京慈恵会医科大学, 医学部, 講師 (30435531) ; 孫 在隣 生理学研究所, 脳機能計測・支援センター, 特任研究員 (40780333) ; 窪田 芳之 生理学研究所, 基盤神経科学研究領域, 准教授 (90192567) ; 倉本 恵梨子 鹿児島大学, 医歯学域歯学系, 助教 (60467470) ; 植田 禎史 東京女子医科大学, 医学部, 助教 (00511015)
• Project Period (FY): 2017-06-30 – 2022-03-31
• Project Status: Granted (Fiscal Year 2021)
• Budget Amount: ¥113,750,000 (Direct Cost: ¥87,500,000、Indirect Cost: ¥26,250,000); Fiscal Year 2021: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000); Fiscal Year 2020: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2019: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000); Fiscal Year 2018: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000); Fiscal Year 2017: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
• Keywords: 前頭皮質 / 錐体細胞 / 局所回路 / 皮質線条体投射 / 皮質間投射 / ソマトスタチン細胞 / FS細胞 / 振動 / ソマトスタチン / 電子顕微鏡

Outline of Annual Research Achievements

前頭皮質局所サブネットワーク形成の機能的意義の理解と、前頭皮質固有の回路則の解明を目指して、以下の実験・解析を進めた。
(1) 5層終脳内投射錐体細胞(IT細胞)の投射多様性: 前頭皮質5層のIT細胞が電気的・生理的には多様であるが、投射する皮質領野との関係は不明である。この点を明らかにするために、Janelia Research Campusの公開データベースにある、単一IT細胞の皮質内軸索分布を定量解析した。その結果、5層IT細胞の中で、複数の皮質領野を支配するものは１層支配が強く、特定の領野だけを支配するものは１層に軸索をあまり出さないことが分かった。これらの皮質間投射細胞サブタイプの分子発現特異性と結合選択性を解析している。
(2) 前頭皮質ソマトスタチン(SOM)細胞の多様性: 前頭皮質のSOM細胞の機能はあまり解明されていない。新皮質2/3層の主要な投射先は、他の皮質領野である。本研究では前頭皮質2/3層で、発火・形態特性を同定したSOM細胞と皮質間投射錐体細胞の結合関係を調べることで、SOM細胞の機能的多様化と、前頭皮質2/3層興奮・抑制回路の結合原理を明らかにする。そのために、2/3層SOM細胞や皮質間投射錐体細胞の生理特性・分子発現・形態の解明を進めている。この解析に導入したSOM細胞にCreを発現するマウスで、SOM発現特異性を免疫組織化学の手法で調べた。前頭皮質2/3層では5/6層とは異なり、Creがソマトスタチン細胞選択的に発現することを確認した。次に、2/3層SOM細胞からホールセル記録することで、これらが電気生理学的に多様であることが分かった。この生理的多様性と、SOM細胞に発現する複数の分子マーカーとの関係を、記録細胞の免疫組織化学で検索した。電気生理的な性質及び分子マーカー発現が異なる細胞の樹状突起・軸索形態を定量的に比較している。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

新皮質局所サブネットワーク理解の為に明らかにしてきた5層興奮性局所回路と、前頭皮質の機能発現に重要な皮質間トップダウン投射の多様化とを結びつける端緒を掴むことができた。また、2/3層興奮性サブネットワークの抑制性制御を理解するのに必要と考えられるソマトスタチン細胞の多様性を明らかにすることができた。

Strategy for Future Research Activity

これまでに明らかにした前頭皮質2/3層と5層の興奮性局所サブネットワークについて、それらの皮質間投射との関係や抑制性制御を明らかにすることで、前頭皮質の基本回路構造(標準)を明らかにしていきたい。

Research Products

• [Int'l Joint Research] マックスプランク研究所(ドイツ)
• [Journal Article] Deep learning-based point-scanning super-resolution imaging (2021)
  • Author(s): Fang L, Monroe F, Novak SW, Kirk L, Schiavon CR, Yu SB, Zhang T, Wu M, Kastner K, Latif AA, Lin Z, Shaw A, Kubota Y, Mendenhall J, Zhang Z, Pekkurnaz G, Harris K, Howard J, Manor U
  • Journal Title: Nat Methods Volume: 18 Pages: 406-416
  • DOI: 10.1038/s41592-021-01080-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice (2021)
  • Author(s): Dhar A, Kuramoto E, Fukushima M, Iwai H, Yamanaka A, Goto T
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 54 Issue: 1 Pages: 11-19
  • DOI: 10.1267/ahc.20-00036
  • NAID: 130007991342
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Open Access
• [Journal Article] Neurodegeneration of trigeminal mesencephalic neurons by the tooth loss triggers the progression of Alzheimer's disease in 3xTg-AD model mice. (2020)
  • Author(s): Goto T, Kuramoto E, Dhar A, Wang RP-H, Seki H, Iwai H, Yamanaka A, Matsumoto S, Hara H, Michikawa M, Ohyagi Y, Leung WK, Chang RC-C
  • Journal Title: J Alzheimer’s Dis Volume: 76 Pages: 1443-1459
  • DOI: 10.3233/jad-200257
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ipsi- and contralateral corticocortical projection-dependent subcircuits in layer 2 of the rat frontal cortex (2019)
  • Author(s): Ueta Yoshifumi、Sohn Jaerin、Agahari Fransiscus Adrian、Im Sanghun、Hirai Yasuharu、Miyata Mariko、Kawaguchi Yasuo
  • Journal Title: J Neurophysiol Volume: 122 Pages: 1461-1472
  • DOI: 10.1152/jn.00333.2019
  • Peer Reviewed
• [Journal Article] Semaphorin 6A?Plexin A2/A4 Interactions with Radial Glia Regulate Migration Termination of Superficial Layer Cortical Neurons (2019)
  • Author(s): Hatanaka Yumiko、Kawasaki Takahiko、Abe Takaya、Shioi Go、Kohno Takao、Hattori Mitsuharu、Sakakibara Akira、Kawaguchi Yasuo、Hirata Tatsumi
  • Journal Title: iScience Volume: 21 Pages: 359-374
  • DOI: 10.1016/j.isci.2019.10.034
  • Peer Reviewed / Open Access
• [Journal Article] Differential Striatal Axonal Arborizations of the Intratelencephalic and Pyramidal-Tract Neurons: Analysis of the Data in the MouseLight Database (2019)
  • Author(s): Kenji Morita, Sanghun Im, Yasuo Kawaguchi
  • Journal Title: Front Neural Circuits Volume: 13 Pages: 71-71
  • DOI: 10.3389/fncir.2019.00071
  • Peer Reviewed / Open Access
• [Journal Article] UNI-EM: An Environment for Deep Neural Network-Based Automated Segmentation of Neuronal Electron Microscopic Images (2019)
  • Author(s): Urakubo Hidetoshi、Bullmann Torsten、Kubota Yoshiyuki、Oba Shigeyuki、Ishii Shin
  • Journal Title: Sci Rep Volume: 9 Pages: 19413-19413
  • DOI: 10.1038/s41598-019-55431-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Control of excitatory hierarchical circuits by parvalbumin-FS basket cells in layer 5 of the frontal cortex: insights for cortical oscillations (2019)
  • Author(s): Kawaguchi Y, Otsuka T, Morishima M, Ushimaru M, Kubota Y
  • Journal Title: J Neurophysiol Volume: 121 Pages: 2222-2236
  • DOI: 10.1152/jn.00778.2018
  • NAID: 120006649569
  • Peer Reviewed / Open Access
• [Journal Article] Method for Labeling and Reconstruction of Single Neurons Using Sindbis Virus Vectors (2019)
  • Author(s): Eriko Kuramoto
  • Journal Title: J Chem Neuroanat Volume: 100 Pages: 101648-101648
  • DOI: 10.1016/j.jchemneu.2019.05.002
  • Peer Reviewed
• [Journal Article] A Dual Role Hypothesis of the Cortico-Basal-Ganglia Pathways: Opponency and Temporal Difference Through Dopamine and Adenosine (2019)
  • Author(s): Kenji Morita, Yasuo Kawaguchi
  • Journal Title: Frontiers in Neural Circuits Volume: 12 Pages: 111-111
  • DOI: 10.3389/fncir.2018.00111
  • Peer Reviewed / Open Access
• [Journal Article] Monitoring and updating of action selection for goal-directed behavior through the striatal direct and indirect pathways (2018)
  • Author(s): Nonomura S, Nishizawa K, Sakai Y, Kawaguchi Y, Kato S, Uchigashima M, Watanabe M, Yamanaka K, Enomoto K, Chiken S, Sano H, Soma S, Yoshida J, Samejima K, Ogawa M, Kobayashi K, Nambu A, Isomura Y, Kimura M
  • Journal Title: Neuron Volume: 99(6) Issue: 6 Pages: 1302-1314
  • DOI: 10.1016/j.neuron.2018.08.002
  • NAID: 120006869035
  • Peer Reviewed / Open Access
• [Journal Article] Thalamocortical Axonal Activity in Motor Cortex Exhibits Layer-Specific Dynamics during Motor Learning (2018)
  • Author(s): Tanaka Yasuyo H.、Tanaka Yasuhiro R.、Kondo Masashi、Terada Shin-Ichiro、Kawaguchi Yasuo、Matsuzaki Masanori
  • Journal Title: Neuron Volume: 100 Pages: 244-258
  • DOI: 10.1016/j.neuron.2018.08.016
  • NAID: 40021939701
  • Peer Reviewed / Open Access
• [Journal Article] New neurons use Slit-Robo signaling to migrate through the glial meshwork and approach a lesion for functional regeneration (2018)
  • Author(s): Kaneko N., Herranz-Perez V., Otsuka T., Sano H., Ohno N., Omata T., Nguyen H. B., Thai T. Q., Nambu A., Kawaguchi Y., Garcia-Verdugo J. M., Sawamoto K.
  • Journal Title: Science Advances Volume: 4 Pages: 0618-0618
  • DOI: 10.1126/sciadv.aav0618
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Large Volume Electron Microscopy and Neural Microcircuit Analysis (2018)
  • Author(s): Kubota Yoshiyuki、Sohn Jaerin、Kawaguchi Yasuo
  • Journal Title: Frontiers in Neural Circuits Volume: 12 Pages: 98-98
  • DOI: 10.3389/fncir.2018.00098
  • Peer Reviewed / Open Access
• [Journal Article] 電子顕微鏡を使った革新的脳組織解析法 ― コネクトーム研究 (2018)
  • Author(s): 窪田芳之, 川口泰雄
  • Journal Title: 実験医学 Volume: 36 Pages: 158-164
• [Journal Article] A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure (2018)
  • Author(s): Kubota Y, Sohn J, Hatada S, Schurr M, StraehleJ, Gour A, Neujahr R, MikiT, Mikula S, Kawaguchi Y
  • Journal Title: Nature Commun Volume: 9 Pages: 437-437
  • DOI: 10.1038/s41467-017-02768-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Segregated Excitatory?Inhibitory Recurrent Subnetworks in Layer 5 of the Rat Frontal Cortex (2017)
  • Author(s): Morishima Mieko、Kobayashi Kenta、Kato Shigeki、Kobayashi Kazuto、Kawaguchi Yasuo
  • Journal Title: Cereb. Cortex Volume: 27 Pages: 5846-5857
  • DOI: 10.1093/cercor/bhx276
  • Peer Reviewed / Open Access
• [Journal Article] Pyramidal cell subtypes and their synaptic connections in layer 5 of rat frontal cortex (2017)
  • Author(s): Kawaguchi Y
  • Journal Title: Cereb Cortex Volume: 27 Pages: 5755-5771
  • DOI: 10.1093/cercor/bhx252
  • Peer Reviewed / Open Access
• [Journal Article] 大脳皮質局所回路：古典的競合選択モデルの実験的検証と混合選択性細胞 (2017)
  • Author(s): 森田賢治, 川口泰雄
  • Journal Title: 生体の科学 Volume: 68 Pages: 43-47
• [Journal Article] Dorsal and ventral parts of thalamic nucleus submedius project to different areas of rat orbitofrontal cortex: A single neuron-tracing study using virus vectors (2017)
  • Author(s): Kuramoto E, Iwai H, Yamanaka A, Ohno S, Seki H, Tanaka Y, Furuta T, Hioki H, Goto T
  • Journal Title: J Comp Neurol Volume: 525 Pages: 3821-3839
  • DOI: 10.1002/cne.12646
  • Peer Reviewed
• [Journal Article] Individual Mediodorsal Thalamic Neurons Project to Multiple Areas of the Rat Prefrontal Cortex: A Single Neuron-Tracing Study Using Virus Vectors. (2017)
  • Author(s): Kuramoto E, Pan SX, Furuta T, Tanaka YR, Iwai H, Yamanaka A, Ohno S, Kaneko T, Goto T, Hioki H.
  • Journal Title: The Journal of comparative neurology Volume: 525 Pages: 166-185
  • DOI: 10.1002/cne.24054
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Synaptic reconfiguration in motor cortex underlies acquisition of proficient motor skill (2021)
  • Author(s): Sohn J, Suzuki M, Mohammed Y, Hatada S, Larkum ME, Kawaguchi Y, Kubota Y
  • Organizer: 第10回 生理研-霊長研-新潟脳研合同シンポジウム
• [Presentation] ラットの大脳皮質細胞へのシナプス入力特性 (2021)
  • Author(s): 窪田芳之, 川口泰雄
  • Organizer: 第126回日本解剖学会総会・全国学術集会／第98回日本生理学会大会
• [Presentation] 運動学習時の皮質－皮質間連絡・視床－皮質投射の神経回路再編成 (2021)
  • Author(s): 孫在隣, 川口泰雄, 窪田芳之
  • Organizer: 第126回日本解剖学会総会・全国学術集会／第98回日本生理学会大会
• [Presentation] 皮質－皮質間連絡・視床－皮質投射の入力依存的な運動学習時回路動態 (2020)
  • Author(s): 孫在隣, Mohammed Y, 川口泰雄, 窪田芳之
  • Organizer: 第43回日本神経科学大会
• [Presentation] 大容量電顕データセットを使った視床-大脳皮質神経終末のシナプスターゲット解析 (2020)
  • Author(s): 窪田芳之, 孫在隣, 倉本恵梨子, Mohammed Y, 日置寛之, 川口泰雄
  • Organizer: 第43回日本神経科学大会
• [Presentation] 導電性カーボンナノチューブコートPETテープに乗せた神経組織の高解像度電子顕微鏡撮影とその解析法 (2020)
  • Author(s): 窪田芳之
  • Organizer: 第59回 フラーレン・ナノチューブ・グラフェン総合シンポジウム
  • Invited
• [Presentation] 非錐体細胞の樹状突起へのシナプス入力特性 (2020)
  • Author(s): 窪田芳之, 川口泰雄
  • Organizer: 第10 回名古屋大学医学系研究科・生理学研究所合同シンポジウム
• [Presentation] Thalamo-cortical synaptic network in motor cortex analyzed with large volume electron microscopy (2020)
  • Author(s): Kubota Y, Sohn J, Kuramoto E, Kawaguchi Y
  • Organizer: The Eighth International Neural Microcircuit Conference- Synaptic Specificity to Circuit Dynamics -
  • Int'l Joint Research
• [Presentation] Sublayer dependent excitatory and inhibitory circuits in the layer 5 of rat frontal cortex (2020)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: The Eighth International Neural Microcircuit Conference- Synaptic Specificity to Circuit Dynamics -
  • Int'l Joint Research
• [Presentation] Input-dependent spine dynamics in motor cortex during motor learning (2020)
  • Author(s): Sohn J, Youssef M, Hatada S, Kawaguchi Y, Kubota Y
  • Organizer: The Eighth International Neural Microcircuit Conference- Synaptic Specificity to Circuit Dynamics -
  • Int'l Joint Research
• [Presentation] Connection selectivity of somatostatin cell subtypes in layer 2/3 of frontal cortex (2020)
  • Author(s): Agahari FA, Kawaguchi Y
  • Organizer: The Eighth International Neural Microcircuit Conference- Synaptic Specificity to Circuit Dynamics -
  • Int'l Joint Research
• [Presentation] Layer 1 projection diversity of layer 5 pyramidal cells in the frontal cortex (2020)
  • Author(s): Im S, Morishima M, Kawaguchi Y
  • Organizer: The Eighth International Neural Microcircuit Conference- Synaptic Specificity to Circuit Dynamics -
  • Int'l Joint Research
• [Presentation] Local circuits in the frontal cortex drive diverse corticocortical projections (2020)
  • Author(s): Kawaguchi Y, Ueta Y, Agahari FA, Im S
  • Organizer: The Eighth International Neural Microcircuit Conference- Synaptic Specificity to Circuit Dynamics -
  • Int'l Joint Research / Invited
• [Presentation] スパインと電気結合：濱先生と私 (2020)
  • Author(s): 川口泰雄
  • Organizer: ABiS Symposium Forefront and Future of Electron Microscopic Imaging
  • Invited
• [Presentation] Thalamo-cortical synaptic network in motor cortex analyzed with large volume electron microscopy (2020)
  • Author(s): Kubota Y, Sohn J, Kuramoto E, Kawaguchi Y
  • Organizer: ABiS Symposium Forefront and Future of Electron Microscopic Imaging
  • Int'l Joint Research
• [Presentation] Network plastic changes in the primary motor cortex as the basis of a new motor skill acquisition (2020)
  • Author(s): Kubota Y, Sohn J, Suzuki M, Hatada S, Youssef M, Larkum ME, Kawaguchi Y
  • Organizer: 2020 Gordon Research Conference, Thamamocortiacl Interaction
  • Int'l Joint Research
• [Presentation] Distinct functional and morphological properties of the frontal and parietal layer 5 corticothalamic pathways (2020)
  • Author(s): Kuramoto E, Bokor H, Hadinger N, Bosz E, Toth B, David C, Komlosi G, Shepherd G, Yamawaki N, Goto T, Acsady L
  • Organizer: 2020 Gordon Research Conference, Thamamocortiacl Interactions
  • Int'l Joint Research
• [Presentation] 運動学習中の大脳皮質入出力の層特異的な神経活動ダイナミクス (2020)
  • Author(s): 田中康裕、田中康代、正水芳人、川口泰雄、松崎政紀
  • Organizer: 第125回日本解剖学会総会・全国学術集会
• [Presentation] 皮質－皮質間連絡・視床－皮質投射の入力依存的な運動学習時回路動態 (2020)
  • Author(s): 孫在隣、窪田芳之、川口泰雄
  • Organizer: 日本顕微鏡学会第75回学術講演会
• [Presentation] ラットの前頭皮質における視床前腹側核VA由来神経終末のシナプスターゲット構造－ATUM-SEM法による解析－ (2020)
  • Author(s): Kubota Y, Sohn J, Kuramoto E, Hioki H, Kawaguchi Y
  • Organizer: 日本顕微鏡学会第75回学術講演会
• [Presentation] Thalamo-cortical synaptic network in motor cortex analyzed with large volume electron microscopy (2019)
  • Author(s): Kubota Y, Sohn J, Kuramoto E, Kawaguchi Y
  • Organizer: 2019 Gordon Research Conference Dendrites: Molecules, Structure and Function
  • Int'l Joint Research
• [Presentation] An electrodeposited copper tape for serial-section electron microscopy of neural ultrastructure with ATUM-SEM (2019)
  • Author(s): Kubota Y, Kawaguchi Y
  • Organizer: 日本顕微鏡学会第75回学術講演会
• [Presentation] The role of cortical pyramidal cell subtype dependent oscillatory activities in motor learning (2019)
  • Author(s): Otsuka T, Kawaguchi Y
  • Organizer: 第42回日本神経科学大会
• [Presentation] Electrophysiological, morphological, and connection characteristics of layer 2/3 somatostatin cells in mouse frontal cortex (2019)
  • Author(s): Agahari FA, Kawaguchi Y
  • Organizer: 第42回日本神経科学大会
• [Presentation] Sublayer-dependent excitatory and inhibitory circuits in layer 5 of the rat frontal cortex (2019)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: 第42回日本神経科学大会
• [Presentation] Corticocortical and thalamocortical remodeling in the cortical microcircuit during motor learning (2019)
  • Author(s): Sohn J, Kubota Y, Kawaguchi Y
  • Organizer: 第42回日本神経科学大会
• [Presentation] An electrodeposited copper foil tape for serial-section electron microscopy of brain ultrastructure with ATUM-SEM (2019)
  • Author(s): Kubota Y, Kawaguchi Y
  • Organizer: 第42回日本神経科学大会
• [Presentation] Coactosin functions as a morphological determinant in developing cortical interneurons (2019)
  • Author(s): Hou X, Morishima M, Matohara N, Sugi J, Sakimura K, Kawaguchi Y, Sugiyama S
  • Organizer: 第42回日本神経科学大会
• [Presentation] Molecular mechanism of KCNQ channels for reward behavior (2019)
  • Author(s): Tsuboi D, Nishioka T, Shimomura T, Otsuka T, Kubo Y, Kawaguchi Y, Kaibuchi K
  • Organizer: 第42回日本神経科学大会
• [Presentation] Cortical oscillatory activities regulate motor learning formation (2019)
  • Author(s): Otsuka T, Kawaguchi Y
  • Organizer: 第11回光操作研究会
• [Presentation] 皮質オシレーション活動による運動学習形成の制御 (2019)
  • Author(s): 大塚岳、川口泰雄
  • Organizer: 第9回名古屋大学医学系研究科・生理学研究所合同シンポジウム
• [Presentation] Connection selectivity of somatostatin cell subtypes in layer 2/3 of frontal cortex (2019)
  • Author(s): Agahari FA, Kawaguchi Y
  • Organizer: 第9回 名古屋大学医学系研究科・生理学研究所合同シンポジウム
• [Presentation] Circuit remodeling in the motor cortex during motor learning (2019)
  • Author(s): Sohn J, Kubota Y, Kawaguchi Y
  • Organizer: 49th annual meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Thalamo cortical synaptic network in motor cortex analyzed with large volume electron microscopy (2019)
  • Author(s): Kubota Y, Sohn J, Kuramoto E, Kawaguchi Y
  • Organizer: 49th annual meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Mapping learning-dependent structural changes in thalamocortical synapses (2019)
  • Author(s): Kawaguchi Y
  • Organizer: The 9th Okazaki-Tuebingen-Beijing Joint Symposium
  • Int'l Joint Research
• [Presentation] Network plastic changes in the primary motor cortex as the basis of a new motor skill acquisition (2019)
  • Author(s): Sohn J, Kubota Y, Kawaguchi Y
  • Organizer: The 9th Okazaki-Tuebingen-Beijing Joint Symposium
  • Int'l Joint Research
• [Presentation] 神経組織の大容量電顕データセット作成とオートセグメンテーションによる３次元再構築 (2019)
  • Author(s): 窪田芳之, 川口泰雄
  • Organizer: 日本顕微鏡学会第62回シンポジウム
• [Presentation] Sublayer- dependent inhibitory-excitatory connections in the layer 5 of rat frontal cortex (2019)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: 第8回生理研-霊長研-新潟脳研合同シンポジウム
• [Presentation] Sublayer- dependent inhibitory-excitatory connections in the layer 5 of rat frontal cortex (2019)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: The 9th BRI International Symposium
  • Int'l Joint Research
• [Presentation] Thalamo-cortical target analysis in cortical motor cortex using large volume electron microscopy (2019)
  • Author(s): Kubota Y, Sohn J, Kuramoto E, Kawaguchi Y
  • Organizer: 第124回日本解剖学会総会・全国学術集会
  • Invited
• [Presentation] Large volume electron microscopy and neural microcircuitanalysis (2019)
  • Author(s): Kubota Y, Sohn J, Kawaguchi Y
  • Organizer: 9th FAOPS
  • Int'l Joint Research
• [Presentation] Layer 5 sublayer-dependent excitatory-inhibitory connections in the rat frontal cortex (2019)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: 9th FAOPS
  • Int'l Joint Research
• [Presentation] Thalamocortical axon activity in motor cortex exhibits layer-specific dynamics during motor learning (2019)
  • Author(s): Tanaka YR, Tanaka YH, Kondo M, Terada S, Kawaguchi Y, Matsuzaki M
  • Organizer: 9th FAOPS
  • Int'l Joint Research
• [Presentation] Sublayer-dependent interaction between GABAergic and pyramidal cells in layer 5 of the rat frontal cortex (2018)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: 第10回光操作研究会・第２回脳情報動態合同国際シンポジウム
  • Int'l Joint Research
• [Presentation] Layer 5a and 5b dependent inhibitory connections in the rat frontal cortex (2018)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: 第41回日本神経科学大会
• [Presentation] Emerging dynamics of thalamocortical ensemble activity through learning of self-initiated lever pull (2018)
  • Author(s): Tanaka YH, Tanaka YR, Kondo M, Terada S, Kawaguchi Y, Matsuzaki M
  • Organizer: 第41回日本神経科学大会
• [Presentation] SemaphorinA6 on radial glia cell and PlexinA2/A4 on neuron are required for proper positioning of superficial layer neurons in the cerebral cortex (2018)
  • Author(s): Hatanaka Y, Kawasaki T, KohnoT, Hattori M, Sakakibara A, Kawaguchi Y, Hirata T
  • Organizer: 第41回日本神経科学大会
• [Presentation] Identification of indirect pathway neurons and dopaminergic innervation in the rat dorsomedial striatum (2018)
  • Author(s): Nonomura S, Kato S, Iguchi Y, Nishizawa K, Sakai Y, Kawaguchi Y, Nambu A, Watanabe M, Kobayashi K, Isomura Y, Kimura M
  • Organizer: 第41回日本神経科学大会
• [Presentation] Cortical neuron types and their synaptic connections (2018)
  • Author(s): Kawaguchi Y
  • Organizer: 第41回日本神経科学大会
  • Invited
• [Presentation] 新皮質パルブアルブミン細胞とソマトスタチン細胞のシナプス構築 (2018)
  • Author(s): 川口泰雄
  • Organizer: 第8回名古屋大学医学系研究科・生理学研究所合同シンポジウム
  • Invited
• [Presentation] A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure (2018)
  • Author(s): Kubota Y, Sohn J, Kawaguchi Y
  • Organizer: 第8回名古屋大学医学系研究科・生理学研究所合同シンポジウム
• [Presentation] Local recurrent subnetworks correlated with long-distance projections in frontal cortex (2018)
  • Author(s): Kawaguchi Y
  • Organizer: International symposium on Brain Information Dynamics 2018
  • Int'l Joint Research / Invited
• [Presentation] Ipsilateral and contralateral corticocortical projection-dependent subcircuits in layer 2/3 of rat frontal cortex (2018)
  • Author(s): Ueta Y, Kawaguchi Y
  • Organizer: 第95回日本生理学会大会
• [Presentation] Dynamics of thalamocortical activities during motor execution and learning (2018)
  • Author(s): Tanaka YH, Tanaka YR, Kondo M, Terada S, Kawaguchi Y, Matsuzaki M
  • Organizer: 第95回日本生理学会大会
• [Presentation] Local connections of excitatory neurons to parvalbumin-containing interneurons in motor-associated cortical areas of mice (2018)
  • Author(s): Kuramoto E
  • Organizer: Gordon Research Conference Thalamocortical Interaction
  • Int'l Joint Research
• [Presentation] ATUMtome 用の新開発テープと高解像画像用組織処理法 (2017)
  • Author(s): 窪田芳之, 孫在隣, 畑田小百合, 川口泰雄
  • Organizer: 第73回日本顕微鏡学会学術講演会
  • Invited
• [Presentation] Differential involvement of direct- and indirect-pathway spiny projection neurons in action valuation and update in the dorsomedial striatum (2017)
  • Author(s): Kimura M, Nonomura S, Nishizawa K, Kobayashi K, Sakai Y, Kawaguchi Y, Nambu A, Yamanaka K, Isomura Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] A novel tape for ATUMtome and high resolution electron microscopy (2017)
  • Author(s): Kubota Y, Sohn J, Hatada S, Kawaguchi Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] Labeling of neurons derived from intermediate neuronal progenitors in the mouse cerebral cortex (2017)
  • Author(s): Hatanaka Y, Hirata T, Kawaguchi Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] Cortical pyramidal cell-subtype dependent motor learning and oscillatory activities (2017)
  • Author(s): Otsuka T, Kawaguchi Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] Thalamocortical dynamics mediate learning and execution of self-initiated movement (2017)
  • Author(s): Tanaka YH, Tanaka YR, Hira R, Kondo M, Terada S, Kawaguchi Y, Matsuzaki M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Activity of direct- and indirect-pathway striatal projection neurons identified by optogenetics during adaptive choice task with probabilistic reward (2017)
  • Author(s): Nonomura S, Yamanaka K, Nishizawa K, Kobayashi K, Sakai Y, Kawaguchi Y, Nambu A, Isomura Y, Kimura M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Pyramidal cell subtype-dependent inhibitory innervations in layer 5 of the rat frontal cortex (2017)
  • Author(s): Morishima M, Kawaguchi Y
  • Organizer: 第40回日本神経科学大会
• [Presentation] 前頭皮質のニューロン構成と標準回路 (2017)
  • Author(s): 川口泰雄
  • Organizer: 新学術領域研究「脳情報動態を規定する多領野連関と並列処理（脳情報動態）」領域公募説明会＆特別講演会「コネクトミクスからAIへ」
  • Invited
• [Presentation] Action-specific reinforcement and update by direct- and indirect- pathway striatal projection neurons (2017)
  • Author(s): Nonomura S, Nishizawa K, Kobayashi K, Sakai Y, Kawaguchi Y, Nambu A, Yamanaka K, Enomoto K, Isomura Y, Kimura M
  • Organizer: 47th annual meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] A conductive novel tape material and a new staining protocol for volume electron microscopy applications (2017)
  • Author(s): Kubota Y, Sohn J, Hatada S, Schurr M , Straehle J, Gour A. G, Neujahr R, Mikula S, Kawaguchi Y
  • Organizer: 47th annual meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Innervation differences of layer 5a and 5b Martinotti cells in frontal cortex (2017)
  • Author(s): Kawaguchi Y, Morishima M
  • Organizer: 47th annual meeting of the Society for Neuroscience
  • Int'l Joint Research
• [Presentation] 前頭皮質ニューロンと再帰的結合の多様性 (2017)
  • Author(s): 川口泰雄
  • Organizer: 次世代脳プロジェクト冬のシンポジウム2017
  • Invited
• [Presentation] Local connections of excitatory neurons to parvalbumin-containing interneurons in motor-associated cortical areas of mice (2017)
  • Author(s): Kuramoto E, Hiokii H, Tanaka Y, Iwai H, Yamanaka A, Goto T, Kaneko T
  • Organizer: FENS regional meeting
  • Int'l Joint Research
• [Remarks] 生理学研究所大脳神経回路論研究部門
  • URL: http://www.nips.ac.jp/circuit/