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Analysis of "Response zone" and " Communication zone" in mitochondria and Golgi apparatus

Planned

Project AreaToward an integrative understanding of functional zones in organelles
Project/Area Number 17H06414
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTokyo Medical and Dental University

Principal Investigator

清水 重臣  東京医科歯科大学, 難治疾患研究所, 教授 (70271020)

Co-Investigator(Kenkyū-buntansha) 田村 康  山形大学, 理学部, 教授 (50631876)
細谷 孝充  東京医科歯科大学, 生体材料工学研究所, 教授 (60273124)
吉田 秀郎  兵庫県立大学, 理学研究科, 教授 (60378528)
矢木 宏和  名古屋市立大学, 医薬学総合研究院(薬学), 講師 (70565423)
Project Period (FY) 2017-06-30 – 2022-03-31
Project Status Granted (Fiscal Year 2021)
Budget Amount *help
¥253,890,000 (Direct Cost: ¥195,300,000、Indirect Cost: ¥58,590,000)
Fiscal Year 2021: ¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000)
Fiscal Year 2020: ¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2019: ¥43,810,000 (Direct Cost: ¥33,700,000、Indirect Cost: ¥10,110,000)
Fiscal Year 2018: ¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000)
Fiscal Year 2017: ¥71,630,000 (Direct Cost: ¥55,100,000、Indirect Cost: ¥16,530,000)
Keywords細胞生物 / オルガネラ / ミトコンドリア / ゴルジ体 / ゾーン / 小胞体 / ストレス
Outline of Annual Research Achievements

1つのオルガネラの中には、異なる役割を担う場が存在し、本領域ではこれをオルガネラ・ゾーンと命名している。本研究では、「ミトコンドリアとゴルジ体の
応答ゾーン」、「ミトコンドリアと小胞体、ゴルジ体の連携ゾーン」を中心に解析を行なった。1、ゴルジ体応答ゾーン:このゾーンには、ゴルジ体ストレス時に、過剰な分泌たんぱく質の蓄積を抑制するためのGOMEDゾーン、タンパク質の転写翻訳制御するゾーン、ゴルジ体分子の運搬を制御するゾーンなどが存在する。(1)GOMEDに関しては、新規分子を3分子同定することに成功した他、初期分子機構の同定に成功した。即ち、(A)GOMEDは、Ulk1分子の746セリンのリン酸化を引き金として実行されること、(B)リン酸化Ulk1は、ゴルジ体に移動して、GOMEDを実行すること、(C)このUlk1リン酸化は、RIPK3によって実行されること、(D)GOMEDが実行されると、ゴルジ体から運搬される分子が分解されること、などを見出した。(2)転写翻訳制御ゾーンに関しては、転写因子としてKLF2を同定し、その活性制御機構を解析した結果、KLF2遺伝子が転写誘導を受けることを見出した。(3)ゴルジ体選別輸送ゾーンに関しては、内在性の糖転移酵素を対象とした選別輸送ゾーンの観察を行うことで、同じゴルジ槽に存在する糖転移酵素同士が異なるゾーンに局在することを発見した。2、ミトコンドリア・小胞体(Mt-ER)連携ゾーン:Split-GFPを用いた遺伝学スクリーニング,Split-APEX2を用いた生化学的解析により小胞体-ミトコンドリア間結合因子および結合調節因子の同定と機能解析を行い候補因子の同定に成功した。3、オルガネラゾーン研究に関わるケミカルバイオロジー:オルガネラ研究への応用が期待されるアルケニルアジドの環状アルキンとのクリック反応性について新知見を得た。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

ゴルジ体のストレス応答ゾーン解析においては、関連分子の同定、実行機構の詳細なメカニズム解明、遺伝学的解析(ゾーン形成ができないマウスの作成)、転写レベルでの解析、数理解析などが順調に進行し、多方面からの解析による相乗的効果が現れ、実態解明が急速に進行した。Mt-ER連携ゾーン解析に関しても、Split-GFPを用いた遺伝学スクリーニング,Split-APEX2を用いた生化学的解析を用いて、ゾーン構成因子の同定や機能解析に成功し、革新的な知見を得つつある。

Strategy for Future Research Activity

ゴルジストレス応答ゾーンやMt-ER連携ゾーンの解析に関しては、ゾーンの実態解明に成功しつつある。研究代表者と分担者の間での有機的な連携により、今後、ゾーンの生理的、病理的な意義の解明を進める予定である。また、研究手法の開発により、新たなオルガネラゾーンも見出されつつあり、これらの実態解明も行っていく。

Report

(3 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report

Research Products

(92 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (5 results) Journal Article (63 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 63 results,  Open Access: 8 results) Presentation (15 results) (of which Int'l Joint Research: 10 results,  Invited: 11 results) Remarks (7 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Pen State University(米国)

    • Country Name
      U.S.A.
    • Counterpart Institution
      Pen State University
    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Yonsei University(韓国)

    • Country Name
      KOREA (REP. OF KOREA)
    • Counterpart Institution
      Yonsei University
    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Kings coledge of London(英国)

    • Country Name
      UNITED KINGDOM
    • Counterpart Institution
      Kings coledge of London
    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Monash University(Australia)

    • Country Name
      Australia
    • Counterpart Institution
      Monash University
    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] National University of Singapore(Singapore)

    • Country Name
      Singapore
    • Counterpart Institution
      National University of Singapore
    • Related Report
      2017 Annual Research Report
  • [Journal Article] The mitochondrial inner membrane protein LETM1 modulates cristae organization through its LETM domain2020

    • Author(s)
      Nakamura Seiko、Matsui Aiko、Akabane Shiori、Tamura Yasushi、Hatano Azumi、Miyano Yuriko、Omote Hiroshi、Kajikawa Mizuho、Maenaka Katsumi、Moriyama Yoshinori、Endo Toshiya、Oka Toshihiko
    • Journal Title

      Communications Biology

      Volume: 3 Pages: 99

    • DOI

      10.1038/s42003-020-0832-5

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for interorganelle phospholipid transport mediated by VAT-12020

    • Author(s)
      Watanabe Yasunori、Tamura Yasushi、Kakuta Chika、Watanabe Seiya、Endo Toshiya
    • Journal Title

      Journal of Biological Chemistry

      Volume: 295 Pages: 3257~3268

    • DOI

      10.1074/jbc.RA119.011019

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD2020

    • Author(s)
      George Ginto、Ninagawa Satoshi、Yagi Hirokazu、Saito Taiki、Ishikawa Tokiro、Sakuma Tetsushi、Yamamoto Takashi、Imami Koshi、Ishihama Yasushi、Kato Koichi、Okada Tetsuya、Mori Kazutoshi
    • Journal Title

      eLife

      Volume: 9 Pages: e53455

    • DOI

      10.7554/eLife.53455

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor2020

    • Author(s)
      Yagi Hirokazu、Yagi-Utsumi Maho、Honda Rena、Ohta Yusaku、Saito Taiki、Nishio Miho、Ninagawa Satoshi、Suzuki Kousuke、Anzai Takahiro、Kamiya Yukiko、Aoki Kazuhiro、Nakanishi Mahito、Satoh Tadashi、Kato Koichi
    • Journal Title

      Nature Communications

      Volume: 11 Pages: -

    • DOI

      10.1038/s41467-020-15192-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The ceramide analogue N-(1-hydroxy-3-morpholino-1-phenylpropan-2-yl)decanamide induces large lipid droplet accumulation and highlights the effect of LAMP-2 deficiency on lipid droplet degradation2020

    • Author(s)
      Kato Yuji、Arakawa Satoko、Terasawa Kazue、Inokuchi Jin-ichi、Iwata Takanori、Shimizu Shigeomi、Watabe Tetsuro、Hara-Yokoyama Miki
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 30 Pages: 126891~126891

    • DOI

      10.1016/j.bmcl.2019.126891

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ER-resident sensor PERK is essential for mitochondrial thermogenesis in brown adipose tissue2020

    • Author(s)
      Kato Hironori、Okabe Kohki、Miyake Masato、Hattori Kazuki、Fukaya Tomohiro、Tanimoto Kousuke、Beini Shi、Mizuguchi Mariko、Torii Satoru、Arakawa Satoko、Ono Masaya、Saito Yusuke、Sugiyama Takashi、Funatsu Takashi、Sato Katsuaki、Shimizu Shigeomi、Oyadomari Seiichi、Ichijo Hidenori、Kadowaki Hisae、Nishitoh Hideki
    • Journal Title

      Life Science Alliance

      Volume: 3 Pages: e201900576

    • DOI

      10.26508/lsa.201900576

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of a phosphorylation site on Ulk1 required for genotoxic stress-induced alternative autophagy2020

    • Author(s)
      Torii Satoru、Yamaguchi Hirofumi、Nakanishi Akira、Arakawa Satoko、Honda Shinya、Moriwaki Kenta、Nakano Hiroyasu、Shimizu Shigeomi
    • Journal Title

      Nature Communications

      Volume: 11 Pages: press

    • DOI

      10.1038/s41467-020-15577-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sanguisorba officinalis L. derived from herbal medicine prevents intestinal inflammation by inducing autophagy in macrophages2020

    • Author(s)
      4.Yasueda A, Kayama H, Murohashi M, Nishimura J, Wakame K, Komatsu K, Ogino T, Miyoshi N, Takahashi H, Uemura M, Matsuda C, Kitagawa T, Takeda K, Ito T, Doki Y, Eguchi H, Shimizu S, Mizushima T
    • Journal Title

      Scientific Reports

      Volume: press Pages: press

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Msp1 Clears Mistargeted Proteins by Facilitating Their Transfer from Mitochondria to the ER2019

    • Author(s)
      Matsumoto Shunsuke、Nakatsukasa Kunio、Kakuta Chika、Tamura Yasushi、Esaki Masatoshi、Endo Toshiya
    • Journal Title

      Molecular Cell

      Volume: 76 Pages: 191~205.e10

    • DOI

      10.1016/j.molcel.2019.07.006

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae2019

    • Author(s)
      Wang Chenguang、Taki Masayasu、Sato Yoshikatsu、Tamura Yasushi、Yaginuma Hideyuki、Okada Yasushi、Yamaguchi Shigehiro
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 116 Pages: 15817~15822

    • DOI

      10.1073/pnas.1905924116

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Facile Synthesis of Diverse 2,6-Disubstituted Arylsilanes via Silylamination and Silylsulfanylation of Aryne Intermediates Generated from o-Iodoaryl Triflates2019

    • Author(s)
      Nakamura Yu、Ozawa Saki、Yoshida Suguru、Hosoya Takamitsu
    • Journal Title

      Chemistry Letters

      Volume: 48 Pages: 1296~1299

    • DOI

      10.1246/cl.190573

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Resonance on the Clickability of Alkenyl Azides in the Strain-promoted Cycloaddition with Dibenzo-fused Cyclooctynes2019

    • Author(s)
      Yoshida Suguru、Goto Sayuri、Nishiyama Yoshitake、Hazama Yuki、Kondo Masakazu、Matsushita Takeshi、Hosoya Takamitsu
    • Journal Title

      Chemistry Letters

      Volume: 48 Pages: 1038~1041

    • DOI

      10.1246/cl.190400

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Target Identification of Bioactive Compounds by Photoaffinity Labeling Using Diazido Probes.2019

    • Author(s)
      Yoshida, S. and Hosoya, T.
    • Journal Title

      Chapter 14 in Cutting-Edge Organic Synthesis and Chemical Biology of Bioactive Molecules ; The Shape of Organic Synthesis to Come (Ed. Y. Kobayashi Springer Nature Singapore)

      Volume: - Pages: 335-355

    • DOI

      10.1007/978-981-13-6244-6_14

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MGSE Regulates Crosstalk from the Mucin Pathway to the TFE3 Pathway of the Golgi Stress Response2019

    • Author(s)
      Jamaludin Mohamad Ikhwan、Wakabayashi Sadao、Taniguchi Mai、Sasaki Kanae、Komori Ryota、Kawamura Hirotada、Takase Hayataka、Sakamoto Miyu、Yoshida Hiderou
    • Journal Title

      Cell Structure and Function

      Volume: 44 Pages: 137~151

    • DOI

      10.1247/csf.19009

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anticancer saponin OSW-1 is a novel class of selective Golgi stress inducer2019

    • Author(s)
      Kimura Mayu、Sasaki Kanae、Fukutani Yosuke、Yoshida Hiderou、Ohsawa Ikuroh、Yohda Masafumi、Sakurai Kaori
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 29 Pages: 1732~1736

    • DOI

      10.1016/j.bmcl.2019.05.022

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Golgi stress response and organelle zones2019

    • Author(s)
      Sasaki Kanae、Yoshida Hiderou
    • Journal Title

      FEBS Letters

      Volume: 593 Pages: 2330~2340

    • DOI

      10.1002/1873-3468.13554

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organelle Zones2019

    • Author(s)
      Sasaki Kanae、Yoshida Hiderou
    • Journal Title

      Cell Structure and Function

      Volume: 44 Pages: 85~94

    • DOI

      10.1247/csf.19010

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Beclin 1 regulates recycling endosome and is required for skin development in mice2019

    • Author(s)
      Noguchi Saori、Honda Shinya、Saitoh Tatsuya、Matsumura Hiroyuki、Nishimura Emi、Akira Shizuo、Shimizu Shigeomi
    • Journal Title

      Communications Biology

      Volume: 2 Pages: 37

    • DOI

      10.1038/s42003-018-0279-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Alkynyl Sulfides by Copper-Catalyzed Thiolation of Terminal Alkynes Using Thiosulfonates2019

    • Author(s)
      Kanemoto Kazuya、Yoshida Suguru、Hosoya Takamitsu
    • Journal Title

      Organic Letters

      Volume: 21 Pages: 3172~3177

    • DOI

      10.1021/acs.orglett.9b00875

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Facile Synthesis of Diverse o-Iodoaryl Triflates from o-Silylaryl Triflates by Aluminum-Mediated Desilyliodination.2019

    • Author(s)
      Yoshida S, Hazama Y, Kanemoto K, Nakamura Y, *Hosoya T
    • Journal Title

      Chem. Lett

      Volume: 印刷中 Pages: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modular Synthesis of Unsymmetrical Doubly-ring-fused Benzene Derivatives Based on a Sequential Ring Construction Strategy Using Oxadiazinones as a Platform Molecule2019

    • Author(s)
      Meguro Tomohiro、Chen Shengnan、Kanemoto Kazuya、Yoshida Suguru、Hosoya Takamitsu
    • Journal Title

      Chemistry Letters

      Volume: 印刷中 Pages: 印刷中

    • DOI

      10.1246/cl.190118

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A facile preparation of functional cycloalkynes via an azide-to-cycloalkyne switching approach2019

    • Author(s)
      Yoshida Suguru、Kuribara Tomoko、Ito Harumi、Meguro Tomohiro、Nishiyama Yoshitake、Karaki Fumika、Hatakeyama Yasutomo、Koike Yuka、Kii Isao、Hosoya Takamitsu
    • Journal Title

      Chemical Communications

      Volume: 55 Pages: 3556~3559

    • DOI

      10.1039/c9cc01113g

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Divergent Synthesis of Photoaffinity Probe Candidates by Click Reactions of Azido-Substituted Aryltrifluoromethyldiazirines2019

    • Author(s)
      Hosoya Takamitsu、Watanabe Kenji、Tsuda Junpei、Ochiai Hidenori、Niwa Takashi
    • Journal Title

      HETEROCYCLES

      Volume: 99 Pages: 1366~1366

    • DOI

      10.3987/COM-18-S(F)76

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of intracellular targets of a small compound by analyzing peptides presented on MHC class I2019

    • Author(s)
      Sugimoto Yuna、Murohashi Michiko、Arakawa Satoko、Honda Shinya、Shimizu Shigeomi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 508 Pages: 480~486

    • DOI

      10.1016/j.bbrc.2018.11.089

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of cyclooxygenase-2-mediated prostaglandin E2-prostaglandin E receptor 4 signaling in cardiac reprogramming2019

    • Author(s)
      Muraoka Naoto、Nara Kaori、Tamura Fumiya、Kojima Hidenori、Yamakawa Hiroyuki、Sadahiro Taketaro、Miyamoto Kazutaka、Isomi Mari、Haginiwa Sho、Tani Hidenori、Kurotsu Shota、Osakabe Rina、Torii Satoru、Shimizu Shigeomi、Okano Hideyuki、Sugimoto Yukihiko、Fukuda Keiichi、Ieda Masaki
    • Journal Title

      Nature Communications

      Volume: 10 Pages: 679

    • DOI

      10.1038/s41467-019-08626-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Porin Associates with Tom22 to Regulate the Mitochondrial Protein Gate Assembly2019

    • Author(s)
      Sakaue Haruka、Shiota Takuya、Ishizaka Naoya、Kawano Shin、Tamura Yasushi、Tan Kher Shing、Imai Kenichiro、Motono Chie、Hirokawa Takatsugu、Taki Kentaro、Miyata Non、Kuge Osamu、Lithgow Trevor、Endo Toshiya
    • Journal Title

      Molecular Cell

      Volume: 73 Pages: 1044~1055.e8

    • DOI

      10.1016/j.molcel.2019.01.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo2019

    • Author(s)
      Sawasato Katsuhiro、Sato Ryo、Nishikawa Hanako、Iimura Naoki、Kamemoto Yuki、Fujikawa Kohki、Yamaguchi Toshiyuki、Kuruma Yutetsu、Tamura Yasushi、Endo Toshiya、Ueda Takuya、Shimamoto Keiko、Nishiyama Ken-ichi
    • Journal Title

      Scientific Reports

      Volume: 9 Pages: 1372

    • DOI

      10.1038/s41598-018-37809-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myristoyl group-aided protein import into the mitochondrial intermembrane space2019

    • Author(s)
      Ueda Eri、Tamura Yasushi、Sakaue Haruka、Kawano Shin、Kakuta Chika、Matsumoto Shunsuke、Endo Toshiya
    • Journal Title

      Scientific Reports

      Volume: 9 Pages: 1185

    • DOI

      10.1038/s41598-018-38016-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maintenance of Cardiolipin and Crista Structure Requires Cooperative Functions of Mitochondrial Dynamics and Phospholipid Transport2019

    • Author(s)
      Kojima Rieko、Kakimoto Yuriko、Furuta Shiina、Itoh Kie、Sesaki Hiromi、Endo Toshiya、Tamura Yasushi
    • Journal Title

      Cell Reports

      Volume: 26 Pages: 518~528.e6

    • DOI

      10.1016/j.celrep.2018.12.070

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PGSE Is a Novel Enhancer Regulating the Proteoglycan Pathway of the Mammalian Golgi Stress Response2019

    • Author(s)
      Sasaki Kanae、Komori Ryota、Taniguchi Mai、Shimaoka Akie、Midori Sachiko、Yamamoto Mayu、Okuda Chiho、Tanaka Ryuya、Sakamoto Miyu、Wakabayashi Sadao、Yoshida Hiderou
    • Journal Title

      Cell Structure and Function

      Volume: 44 Pages: 1~19

    • DOI

      10.1247/csf.18031

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain2019

    • Author(s)
      Narentuya、Takeda-Uchimura Yoshiko、Foyez Tahmina、Zhang Zui、Akama Tomoya O.、Yagi Hirokazu、Kato Koichi、Komatsu Yukio、Kadomatsu Kenji、Uchimura Kenji
    • Journal Title

      Scientific Reports

      Volume: 9 Pages: 4387

    • DOI

      10.1038/s41598-019-40901-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of the heterogeneity of extracellular vesicles by membrane organization and sorting machineries2019

    • Author(s)
      Harada Yoichiro、Suzuki Takehiro、Fukushige Tomoko、Kizuka Yasuhiko、Yagi Hirokazu、Yamamoto Mika、Kondo Kiyotaka、Inoue Hiromasa、Kato Koichi、Taniguchi Naoyuki、Kanekura Takuro、Dohmae Naoshi、Maruyama Ikuro
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - General Subjects

      Volume: 1863 Pages: 681~691

    • DOI

      10.1016/j.bbagen.2019.01.015

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ligand binding to human prostaglandin E receptor EP4 at the lipid-bilayer interface2018

    • Author(s)
      Toyoda Y、Morimoto K、Suno R、Horita S、Yamashita K、Hirata K、Shimizu T、Asada H、Nakane T、Shiimura Y、Nakagita T、Tsuge K、Yoshida S、Kuribara Tomoko、Hosoya Takamitsu、Sugimoto Y、Nomura N、Sato M、Hirokawa T、Kinoshita M、Murata T、Takayama K、Yamamoto M、Narumiya S、Iwata S、Kobayashi T
    • Journal Title

      Nature Chemical Biology

      Volume: 15 Pages: 18~26

    • DOI

      10.1038/s41589-018-0131-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association between aromatase in human brains and personality traits2018

    • Author(s)
      Takahashi Kayo、Hosoya Takamitsu、Onoe Kayo、Takashima Tadayuki、Tanaka Masaaki、Ishii Akira、Nakatomi Yasuhito、Tazawa Shusaku、Takahashi Kazuhiro、Doi Hisashi、Wada Yasuhiro、Watanabe Yasuyoshi
    • Journal Title

      Scientific Reports

      Volume: 8 Pages: 16841

    • DOI

      10.1038/s41598-018-35065-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Further enhancement of the clickability of doubly sterically-hindered aryl azides by para-amino substitution2018

    • Author(s)
      Yoshida Suguru、Tanaka Junko、Nishiyama Yoshitake、Hazama Yuki、Matsushita Takeshi、Hosoya Takamitsu
    • Journal Title

      Chemical Communications

      Volume: 54 Pages: 13499~13502

    • DOI

      10.1039/c8cc05791e

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Palladium(ii)-mediated rapid 11C-cyanation of (hetero)arylborons2018

    • Author(s)
      Zhang Zhouen、Niwa Takashi、Watanabe Yasuyoshi、Hosoya Takamitsu
    • Journal Title

      Organic & Biomolecular Chemistry

      Volume: 16 Pages: 7711~7716

    • DOI

      10.1039/c8ob02049c

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydrosilyl Group-directed Iridium-catalyzed peri-Selective C?H Borylation of Ring-fused (Hetero)Arenes2018

    • Author(s)
      Sumida Yuto、Harada Ryu、Sumida Tomoe、Hashizume Daisuke、Hosoya Takamitsu
    • Journal Title

      Chemistry Letters

      Volume: 47 Pages: 1251~1254

    • DOI

      10.1246/cl.180594

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of Arynes by Selective Cleavage of a Carbon?Phosphorus Bond of o-(Diarylphosphinyl)aryl Triflates Using a Grignard Reagent2018

    • Author(s)
      Nishiyama Yoshitake、Kamada Shuhei、Yoshida Suguru、Hosoya Takamitsu
    • Journal Title

      Chemistry Letters

      Volume: 47 Pages: 1216~1219

    • DOI

      10.1246/cl.180555

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transient Protection of Organic Azides from Click Reactions with Alkynes by Phosphazide Formation2018

    • Author(s)
      Meguro Tomohiro、Yoshida Suguru、Igawa Kazunobu、Tomooka Katsuhiko、Hosoya Takamitsu
    • Journal Title

      Organic Letters

      Volume: 20 Pages: 4126~4130

    • DOI

      10.1021/acs.orglett.8b01692

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expanding the synthesizable multisubstituted benzo[b]thiophenes via 6,7-thienobenzynes generated from o-silylaryl triflate-type precursors2018

    • Author(s)
      Yoshida Suguru、Kuribara Tomoko、Morita Takamoto、Matsuzawa Tsubasa、Morimoto Kazushi、Kobayashi Takuya、Hosoya Takamitsu
    • Journal Title

      RSC Advances

      Volume: 8 Pages: 21754~21758

    • DOI

      10.1039/C8RA04035D

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Staudinger reaction using 2,6-dichlorophenyl azide derivatives for robust aza-ylide formation applicable to bioconjugation in living cells2018

    • Author(s)
      Meguro Tomohiro、Terashima Norikazu、Ito Harumi、Koike Yuka、Kii Isao、Yoshida Suguru、Hosoya Takamitsu
    • Journal Title

      Chemical Communications

      Volume: 54 Pages: 7904~7907

    • DOI

      10.1039/C8CC00179K

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Slow luminescence kinetics of semi-synthetic aequorin: expression, purification and structure determination of cf3-aequorin2018

    • Author(s)
      Inouye Satoshi、Tomabechi Yuri、Hosoya Takamitsu、Sekine Shun-ichi、Shirouzu Mikako
    • Journal Title

      The Journal of Biochemistry

      Volume: 164 Pages: 247~255

    • DOI

      10.1093/jb/mvy049

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CDK9 Inhibitor FIT-039 Suppresses Viral Oncogenes E6 and E7 and Has a Therapeutic Effect on HPV-Induced Neoplasia2018

    • Author(s)
      Ajiro Masahiko、Sakai Hiroyuki、Onogi Hiroshi、Yamamoto Makoto、Sumi Eriko、Sawada Teruo、Nomura Takashi、Kabashima Kenji、Hosoya Takamitsu、Hagiwara Masatoshi
    • Journal Title

      Clinical Cancer Research

      Volume: 24 Pages: 4518~4528

    • DOI

      10.1158/1078-0432.CCR-17-3119

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Diverse Phenothiazines by Direct Thioamination of Arynes with S-(o-Bromoaryl)-S-methylsulfilimines and Subsequent Intramolecular Buchwald?Hartwig Amination2018

    • Author(s)
      Matsuzawa Tsubasa、Uchida Keisuke、Yoshida Suguru、Hosoya Takamitsu
    • Journal Title

      Chemistry Letters

      Volume: 47 Pages: 825~828

    • DOI

      10.1246/cl.180304

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantification of receptor activation by oxytocin and vasopressin in endocytosis-coupled bioluminescence reduction assay using nanoKAZ2018

    • Author(s)
      Kii Isao、Hirahara-Owada Shino、Yamaguchi Masataka、Niwa Takashi、Koike Yuka、Sonamoto Rie、Ito Harumi、Takahashi Kayo、Yokoyama Chihiro、Hayashi Takuya、Hosoya Takamitsu、Watanabe Yasuyoshi
    • Journal Title

      Analytical Biochemistry

      Volume: 549 Pages: 174~183

    • DOI

      10.1016/j.ab.2018.04.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Convergent synthesis of trifunctional molecules by three sequential azido-type-selective cycloadditions2018

    • Author(s)
      Yoshida Suguru、Kanno Kimiyuki、Kii Isao、Misawa Yoshihiro、Hagiwara Masatoshi、Hosoya Takamitsu
    • Journal Title

      Chemical Communications

      Volume: 54 Pages: 3705~3708

    • DOI

      10.1039/c8cc01195h

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association Between Autophagy and Neurodegenerative Diseases2018

    • Author(s)
      Fujikake Nobuhiro、Shin Minkyoung、Shimizu Shigeomi
    • Journal Title

      Frontiers in Neuroscience

      Volume: 12 Pages: 255

    • DOI

      10.3389/fnins.2018.00255

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organelle zones in mitochondria2018

    • Author(s)
      Shimizu Shigeomi
    • Journal Title

      The Journal of Biochemistry

      Volume: 165 Pages: 101~107

    • DOI

      10.1093/jb/mvy068

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery and Optimization of Inhibitors of the Parkinson’s Disease Associated Protein DJ-12018

    • Author(s)
      Tashiro Shinya、Caaveiro Jose M. M.、Nakakido Makoto、Tanabe Aki、Nagatoishi Satoru、Tamura Yasushi、Matsuda Noriyuki、Liu Dali、Hoang Quyen Q.、Tsumoto Kouhei
    • Journal Title

      ACS Chemical Biology

      Volume: 13 Pages: 2783~2793

    • DOI

      10.1021/acschembio.8b00701

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system2018

    • Author(s)
      Kakimoto Yuriko、Tashiro Shinya、Kojima Rieko、Morozumi Yuki、Endo Toshiya、Tamura Yasushi
    • Journal Title

      Scientific Reports

      Volume: 8 Pages: 6175

    • DOI

      10.1038/s41598-018-24466-0

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Biological Approach to Biopharmaceuticals2018

    • Author(s)
      Kato Koichi、Yanaka Saeko、Yagi Hirokazu
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 138 Pages: 1495~1502

    • DOI

      10.1248/yakushi.18-00020-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure and Dynamics of Immunoglobulin G Glycoproteins2018

    • Author(s)
      Yagi Hirokazu、Yanaka Saeko、Kato Koichi
    • Journal Title

      Adv. Exp. Med. Biol.

      Volume: 1104 Pages: 219~235

    • DOI

      10.1007/978-981-13-2158-0_11

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct substrate specificities of human GlcNAc-6-sulfotransferases revealed by mass spectrometry?based sulfoglycomic analysis2018

    • Author(s)
      Yu Shin-Yi、Hsiao Cheng-Te、Izawa Mineko、Yusa Akiko、Ishida Hiroji、Nakamura Shigeo、Yagi Hirokazu、Kannagi Reiji、Khoo Kay-Hooi
    • Journal Title

      Journal of Biological Chemistry

      Volume: 293 Pages: 15163~15177

    • DOI

      10.1074/jbc.RA118.001937

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] N-glycan structures of human alveoli provide insight into influenza A virus infection and pathogenesis2018

    • Author(s)
      Sriwilaijaroen Nongluk、Nakakita Shin-ichi、Kondo Sachiko、Yagi Hirokazu、Kato Koichi、Murata Takeomi、Hiramatsu Hiroaki、Kawahara Toshio、Watanabe Yohei、Kanai Yasushi、Ono Takao、Hirabayashi Jun、Matsumoto Kazuhiko、Suzuki Yasuo
    • Journal Title

      The FEBS Journal

      Volume: 285 Pages: 1611~1634

    • DOI

      10.1111/febs.14431

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems2018

    • Author(s)
      Yanaka Saeko、Yagi Hirokazu、Yogo Rina、Yagi-Utsumi Maho、Kato Koichi
    • Journal Title

      Journal of Biomolecular NMR

      Volume: 71 Pages: 193~202

    • DOI

      10.1007/s10858-018-0169-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dram1 regulates DNA damage-induced alternative autophagy2018

    • Author(s)
      Nagata Meruna、Arakawa Satoko、Yamaguchi Hirofumi、Torii Satoru、Endo Hazuki、Tsujioka Masatsune、Honda Shinya、Nishida Yuya、Konishi Akimitsu、Shimizu Shigeomi
    • Journal Title

      Cell Stress

      Volume: 2 Pages: 55~65

    • DOI

      10.15698/cst2018.03.127

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Small fluorescent molecules for monitoring autophagic flux2018

    • Author(s)
      Iwashita Hidefumi、Sakurai Hajime Tajima、Nagahora Noriyoshi、Ishiyama Munetaka、Shioji Kosei、Sasamoto Kazumi、Okuma Kentaro、Shimizu Shigeomi、Ueno Yuichiro
    • Journal Title

      FEBS Letters

      Volume: 592 Pages: 559~567

    • DOI

      10.1002/1873-3468.12979

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biological Roles of Alternative Autophagy2018

    • Author(s)
      Shigeomi Shimizu
    • Journal Title

      Mol. Cells

      Volume: 41 Pages: 50-54

    • DOI

      10.14348/molcells.2018.2215

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Site-specific N-glycosylation analysis of soluble Fcγ receptor IIIb in human serum2018

    • Author(s)
      Yagi Hirokazu、Takakura Daisuke、Roumenina Lubka T.、Fridman Wolf Herman、Saut?s-Fridman Catherine、Kawasaki Nana、Kato Koichi
    • Journal Title

      Scientific Reports

      Volume: 8 Pages: 2719

    • DOI

      10.1038/s41598-018-21145-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of Atg5-dependent cell death in the embryonic development of Bax/Bak double-knockout mice2017

    • Author(s)
      Arakawa Satoko、Tsujioka Masatsune、Yoshida Tatsushi、Tajima-Sakurai Hajime、Nishida Yuya、Matsuoka Yosuke、Yoshino Ikuyo、Tsujimoto Yoshihide、Shimizu Shigeomi
    • Journal Title

      Cell Death and Differentiation

      Volume: 24 Pages: 1598~1608

    • DOI

      10.1038/cdd.2017.84

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-function insights into direct lipid transfer between membranes by Mmm1-Mdm12 of ERMES2017

    • Author(s)
      Kawano Shin、Tamura Yasushi、Kojima Rieko、Bala Siqin、Asai Eri、Michel Agnes H.、Kornmann Benoit、Riezman Isabelle、Riezman Howard、Sakae Yoshitake、Okamoto Yuko、Endo Toshiya
    • Journal Title

      The Journal of Cell Biology

      Volume: 217 Pages: 959~974

    • DOI

      10.1083/jcb.201704119

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Intra- and Inter-mitochondrial Membrane Contact Sites in Yeast Phospholipid Biogenesis2017

    • Author(s)
      Tamura Yasushi、Endo Toshiya
    • Journal Title

      Adv Exp Med Biol.

      Volume: 997 Pages: 121~133

    • DOI

      10.1007/978-981-10-4567-7_9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lewis X-Carrying Neoglycolipids Evoke Selective Apoptosis in Neural Stem Cells2017

    • Author(s)
      Yagi Hirokazu、Yan Gengwei、Suzuki Tatsuya、Tsuge Shingo、Yamaguchi Takumi、Kato Koichi
    • Journal Title

      Neurochemical Research

      Volume: 43 Pages: 212~218

    • DOI

      10.1007/s11064-017-2415-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Roles of phospholipid transport in mitochondrial biogenesis2019

    • Author(s)
      Yasushi Tamura
    • Organizer
      The International Conference on the Bioscience of Lipids (AMED-Lipid Satellite Symposium)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of novel factors involved in formation of ER-mitochondria contact sites2019

    • Author(s)
      Yasushi Tamura
    • Organizer
      Organelle zones: opening a new era of Cell Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulatory mechanisms controlling the number of ER-mitochondria contact sites2019

    • Author(s)
      Yasushi Tamura
    • Organizer
      Institute of Biochemistry and Molecular Biology, Freiburg University, Research seminar
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Two independent regulatory mechanisms controlling the number of ER-mitochondria contact sites2019

    • Author(s)
      Yasushi Tamura
    • Organizer
      Organelle zones meet compartmental gates and contact sites
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanisms and physiological roles of alternative autophagy2019

    • Author(s)
      Shigeomi Shimizu
    • Organizer
      The 92nd Annual Meeting of the Japanese Biochemical Society
    • Related Report
      2019 Annual Research Report
  • [Presentation] Molecular mechanisms and physiological roles of Atg5-independent mitophagy2019

    • Author(s)
      Shigeomi Shimizu
    • Organizer
      ASMRM & J-mit
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] New insights into the mechanism of Golgi-mediated alternative autophagy using chemical biology approach2019

    • Author(s)
      Shigeomi Shimizu
    • Organizer
      International Symposium of Autophagy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Promotion of α2,3-sialylation of recombinant erythropoietin by tagging it with a 10-aminoacid sequence recognized by a cargo receptor complex2019

    • Author(s)
      Hirokazu Yagi, Rena Honda, Rino Yamada, Maho Yagi-Utsumi1, Kazuhiro Aoki, and Koichi Kato
    • Organizer
      25th International Symposium on Glycoconjugates
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Deciphering the molecular code for FUT9-dependent Lewis X modification2019

    • Author(s)
      Taiki Saito, Hirokazu Yagi, Chuwei Kuo, Kay-Hooi Khoo, and Koichi Kato
    • Organizer
      25th International Symposium on Glycoconjugates
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オートファジーと疾患2018

    • Author(s)
      清水重臣
    • Organizer
      第6回AAA会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] オートファジーが関わる細胞死2017

    • Author(s)
      清水重臣
    • Organizer
      日本Cell Death学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 新規オートファジーの分子機構とその破綻による疾患2017

    • Author(s)
      清水重臣
    • Organizer
      第22回 日本病態プロテアーゼ学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Development of molecularly targeted anticancer agents based on the regulation of autophagic cell death2017

    • Author(s)
      清水重臣
    • Organizer
      第76回日本癌学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Structural and Mechanistic Insights into Phospholipid Transfer via Mitochondria2017

    • Author(s)
      Yasushi Tamura
    • Organizer
      5th International Conference on Smart Systems Engineering
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The characterization of the laminin-binding glycans on α-dystroglycan catalyzed by several causative gene products of dystroglycanopathy2017

    • Author(s)
      Hirokazy Yagi
    • Organizer
      1st International Conference on the Glycobiology of Nervous System
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 東京医科歯科大学 病態細胞生物ホームページ

    • URL

      http://www.tmd.ac.jp/mri/pcb/index.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] オルガネラゾーン ホームページ

    • URL

      http://organellezone.org/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 東京医科歯科大学病態細胞生物ホームページ

    • URL

      http://www.tmd.ac.jp/mri/pcb/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 東京医科歯科大学生命有機化学ホームページ

    • URL

      http://chembiolab.sakura.ne.jp/Home.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 山形大学理学部・田村研究室ホームページ

    • URL

      https://www.tamuralab.com/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 兵庫県立大学大学院生命理学研究科ホームページ

    • URL

      http://www.eonet.ne.jp/~biophysics-kyoto/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 名古屋市立大学 大学院薬学研究科 生命構造学分野ホームページ

    • URL

      http://www.phar.nagoya-cu.ac.jp/hp/sbk/

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 糖タンパク質の糖修飾2018

    • Inventor(s)
      矢木宏和、齋藤泰輝、加藤晃一
    • Industrial Property Rights Holder
      矢木宏和、齋藤泰輝、加藤晃一
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特願2018-047235
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 糖タンパク質の生産方法2017

    • Inventor(s)
      矢木宏和、本田怜奈、加藤晃一
    • Industrial Property Rights Holder
      矢木宏和、本田怜奈、加藤晃一
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特願2017-132312
    • Related Report
      2017 Annual Research Report

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Published: 2017-07-04   Modified: 2022-03-29  

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