Analysis of "Response zone" and " Communication zone" in mitochondria and Golgi apparatus

• Project Area: Toward an integrative understanding of functional zones in organelles
• Project/Area Number: 17H06414
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: 清水 重臣 東京医科歯科大学, 難治疾患研究所, 教授 (70271020)
• Co-Investigator(Kenkyū-buntansha): 田村 康 山形大学, 理学部, 教授 (50631876) ; 細谷 孝充 東京医科歯科大学, 生体材料工学研究所, 教授 (60273124) ; 吉田 秀郎 兵庫県立大学, 理学研究科, 教授 (60378528) ; 矢木 宏和 名古屋市立大学, 医薬学総合研究院(薬学), 講師 (70565423)
• Project Period (FY): 2017-06-30 – 2022-03-31
• Project Status: Granted (Fiscal Year 2021)
• Budget Amount: ¥253,890,000 (Direct Cost: ¥195,300,000、Indirect Cost: ¥58,590,000); Fiscal Year 2021: ¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000); Fiscal Year 2020: ¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000); Fiscal Year 2019: ¥43,810,000 (Direct Cost: ¥33,700,000、Indirect Cost: ¥10,110,000); Fiscal Year 2018: ¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000); Fiscal Year 2017: ¥71,630,000 (Direct Cost: ¥55,100,000、Indirect Cost: ¥16,530,000)
• Keywords: 細胞生物 / オルガネラ / ミトコンドリア / ゴルジ体 / ゾーン / 小胞体 / ストレス

Outline of Annual Research Achievements

１つのオルガネラの中には、異なる役割を担う場が存在し、本領域ではこれをオルガネラ・ゾーンと命名している。本研究では、「ミトコンドリアとゴルジ体の
応答ゾーン」、「ミトコンドリアと小胞体、ゴルジ体の連携ゾーン」を中心に解析を行なった。１、ゴルジ体応答ゾーン：このゾーンには、ゴルジ体ストレス時に、過剰な分泌たんぱく質の蓄積を抑制するためのGOMEDゾーン、タンパク質の転写翻訳制御するゾーン、ゴルジ体分子の運搬を制御するゾーンなどが存在する。(1)GOMEDに関しては、新規分子を３分子同定することに成功した他、初期分子機構の同定に成功した。即ち、(A)GOMEDは、Ulk1分子の746セリンのリン酸化を引き金として実行されること、(B)リン酸化Ulk1は、ゴルジ体に移動して、GOMEDを実行すること、(C)このUlk1リン酸化は、RIPK3によって実行されること、(D)GOMEDが実行されると、ゴルジ体から運搬される分子が分解されること、などを見出した。(2)転写翻訳制御ゾーンに関しては、転写因子としてKLF2を同定し、その活性制御機構を解析した結果、KLF2遺伝子が転写誘導を受けることを見出した。(3)ゴルジ体選別輸送ゾーンに関しては、内在性の糖転移酵素を対象とした選別輸送ゾーンの観察を行うことで、同じゴルジ槽に存在する糖転移酵素同士が異なるゾーンに局在することを発見した。２、ミトコンドリア・小胞体（Mt-ER)連携ゾーン：Split-GFPを用いた遺伝学スクリーニング，Split-APEX2を用いた生化学的解析により小胞体-ミトコンドリア間結合因子および結合調節因子の同定と機能解析を行い候補因子の同定に成功した。３、オルガネラゾーン研究に関わるケミカルバイオロジー：オルガネラ研究への応用が期待されるアルケニルアジドの環状アルキンとのクリック反応性について新知見を得た。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

ゴルジ体のストレス応答ゾーン解析においては、関連分子の同定、実行機構の詳細なメカニズム解明、遺伝学的解析（ゾーン形成ができないマウスの作成）、転写レベルでの解析、数理解析などが順調に進行し、多方面からの解析による相乗的効果が現れ、実態解明が急速に進行した。Mt-ER連携ゾーン解析に関しても、Split-GFPを用いた遺伝学スクリーニング，Split-APEX2を用いた生化学的解析を用いて、ゾーン構成因子の同定や機能解析に成功し、革新的な知見を得つつある。

Strategy for Future Research Activity

ゴルジストレス応答ゾーンやMt-ER連携ゾーンの解析に関しては、ゾーンの実態解明に成功しつつある。研究代表者と分担者の間での有機的な連携により、今後、ゾーンの生理的、病理的な意義の解明を進める予定である。また、研究手法の開発により、新たなオルガネラゾーンも見出されつつあり、これらの実態解明も行っていく。

Research Products

• [Int'l Joint Research] Pen State University(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: Pen State University
• [Int'l Joint Research] Yonsei University(韓国)
  • Country Name: KOREA (REP. OF KOREA)
  • Counterpart Institution: Yonsei University
• [Int'l Joint Research] Kings coledge of London(英国)
  • Country Name: UNITED KINGDOM
  • Counterpart Institution: Kings coledge of London
• [Int'l Joint Research] Monash University(Australia)
  • Country Name: Australia
  • Counterpart Institution: Monash University
• [Int'l Joint Research] National University of Singapore(Singapore)
  • Country Name: Singapore
  • Counterpart Institution: National University of Singapore
• [Journal Article] The mitochondrial inner membrane protein LETM1 modulates cristae organization through its LETM domain (2020)
  • Author(s): Nakamura Seiko、Matsui Aiko、Akabane Shiori、Tamura Yasushi、Hatano Azumi、Miyano Yuriko、Omote Hiroshi、Kajikawa Mizuho、Maenaka Katsumi、Moriyama Yoshinori、Endo Toshiya、Oka Toshihiko
  • Journal Title: Communications Biology Volume: 3 Pages: 99
  • DOI: 10.1038/s42003-020-0832-5
  • Peer Reviewed
• [Journal Article] Structural basis for interorganelle phospholipid transport mediated by VAT-1 (2020)
  • Author(s): Watanabe Yasunori、Tamura Yasushi、Kakuta Chika、Watanabe Seiya、Endo Toshiya
  • Journal Title: Journal of Biological Chemistry Volume: 295 Pages: 3257～3268
  • DOI: 10.1074/jbc.RA119.011019
  • Peer Reviewed
• [Journal Article] EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD (2020)
  • Author(s): George Ginto、Ninagawa Satoshi、Yagi Hirokazu、Saito Taiki、Ishikawa Tokiro、Sakuma Tetsushi、Yamamoto Takashi、Imami Koshi、Ishihama Yasushi、Kato Koichi、Okada Tetsuya、Mori Kazutoshi
  • Journal Title: eLife Volume: 9 Pages: e53455
  • DOI: 10.7554/eLife.53455
  • Peer Reviewed / Open Access
• [Journal Article] Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor (2020)
  • Author(s): Yagi Hirokazu、Yagi-Utsumi Maho、Honda Rena、Ohta Yusaku、Saito Taiki、Nishio Miho、Ninagawa Satoshi、Suzuki Kousuke、Anzai Takahiro、Kamiya Yukiko、Aoki Kazuhiro、Nakanishi Mahito、Satoh Tadashi、Kato Koichi
  • Journal Title: Nature Communications Volume: 11 Pages: -
  • DOI: 10.1038/s41467-020-15192-1
  • Peer Reviewed / Open Access
• [Journal Article] The ceramide analogue N-(1-hydroxy-3-morpholino-1-phenylpropan-2-yl)decanamide induces large lipid droplet accumulation and highlights the effect of LAMP-2 deficiency on lipid droplet degradation (2020)
  • Author(s): Kato Yuji、Arakawa Satoko、Terasawa Kazue、Inokuchi Jin-ichi、Iwata Takanori、Shimizu Shigeomi、Watabe Tetsuro、Hara-Yokoyama Miki
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 30 Pages: 126891～126891
  • DOI: 10.1016/j.bmcl.2019.126891
  • Peer Reviewed
• [Journal Article] ER-resident sensor PERK is essential for mitochondrial thermogenesis in brown adipose tissue (2020)
  • Author(s): Kato Hironori、Okabe Kohki、Miyake Masato、Hattori Kazuki、Fukaya Tomohiro、Tanimoto Kousuke、Beini Shi、Mizuguchi Mariko、Torii Satoru、Arakawa Satoko、Ono Masaya、Saito Yusuke、Sugiyama Takashi、Funatsu Takashi、Sato Katsuaki、Shimizu Shigeomi、Oyadomari Seiichi、Ichijo Hidenori、Kadowaki Hisae、Nishitoh Hideki
  • Journal Title: Life Science Alliance Volume: 3 Pages: e201900576
  • DOI: 10.26508/lsa.201900576
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a phosphorylation site on Ulk1 required for genotoxic stress-induced alternative autophagy (2020)
  • Author(s): Torii Satoru、Yamaguchi Hirofumi、Nakanishi Akira、Arakawa Satoko、Honda Shinya、Moriwaki Kenta、Nakano Hiroyasu、Shimizu Shigeomi
  • Journal Title: Nature Communications Volume: 11 Pages: press
  • DOI: 10.1038/s41467-020-15577-2
  • Peer Reviewed / Open Access
• [Journal Article] Sanguisorba officinalis L. derived from herbal medicine prevents intestinal inflammation by inducing autophagy in macrophages (2020)
  • Author(s): 4.Yasueda A, Kayama H, Murohashi M, Nishimura J, Wakame K, Komatsu K, Ogino T, Miyoshi N, Takahashi H, Uemura M, Matsuda C, Kitagawa T, Takeda K, Ito T, Doki Y, Eguchi H, Shimizu S, Mizushima T
  • Journal Title: Scientific Reports Volume: press Pages: press
  • Peer Reviewed / Open Access
• [Journal Article] Msp1 Clears Mistargeted Proteins by Facilitating Their Transfer from Mitochondria to the ER (2019)
  • Author(s): Matsumoto Shunsuke、Nakatsukasa Kunio、Kakuta Chika、Tamura Yasushi、Esaki Masatoshi、Endo Toshiya
  • Journal Title: Molecular Cell Volume: 76 Pages: 191～205.e10
  • DOI: 10.1016/j.molcel.2019.07.006
  • Peer Reviewed
• [Journal Article] A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae (2019)
  • Author(s): Wang Chenguang、Taki Masayasu、Sato Yoshikatsu、Tamura Yasushi、Yaginuma Hideyuki、Okada Yasushi、Yamaguchi Shigehiro
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 116 Pages: 15817～15822
  • DOI: 10.1073/pnas.1905924116
  • Peer Reviewed / Open Access
• [Journal Article] Facile Synthesis of Diverse 2,6-Disubstituted Arylsilanes via Silylamination and Silylsulfanylation of Aryne Intermediates Generated from o-Iodoaryl Triflates (2019)
  • Author(s): Nakamura Yu、Ozawa Saki、Yoshida Suguru、Hosoya Takamitsu
  • Journal Title: Chemistry Letters Volume: 48 Pages: 1296～1299
  • DOI: 10.1246/cl.190573
  • Peer Reviewed
• [Journal Article] Effect of Resonance on the Clickability of Alkenyl Azides in the Strain-promoted Cycloaddition with Dibenzo-fused Cyclooctynes (2019)
  • Author(s): Yoshida Suguru、Goto Sayuri、Nishiyama Yoshitake、Hazama Yuki、Kondo Masakazu、Matsushita Takeshi、Hosoya Takamitsu
  • Journal Title: Chemistry Letters Volume: 48 Pages: 1038～1041
  • DOI: 10.1246/cl.190400
  • Peer Reviewed
• [Journal Article] Target Identification of Bioactive Compounds by Photoaffinity Labeling Using Diazido Probes. (2019)
  • Author(s): Yoshida, S. and Hosoya, T.
  • Journal Title: Chapter 14 in Cutting-Edge Organic Synthesis and Chemical Biology of Bioactive Molecules ; The Shape of Organic Synthesis to Come (Ed. Y. Kobayashi Springer Nature Singapore) Volume: - Pages: 335-355
  • DOI: 10.1007/978-981-13-6244-6_14
  • Peer Reviewed
• [Journal Article] MGSE Regulates Crosstalk from the Mucin Pathway to the TFE3 Pathway of the Golgi Stress Response (2019)
  • Author(s): Jamaludin Mohamad Ikhwan、Wakabayashi Sadao、Taniguchi Mai、Sasaki Kanae、Komori Ryota、Kawamura Hirotada、Takase Hayataka、Sakamoto Miyu、Yoshida Hiderou
  • Journal Title: Cell Structure and Function Volume: 44 Pages: 137～151
  • DOI: 10.1247/csf.19009
  • Peer Reviewed
• [Journal Article] Anticancer saponin OSW-1 is a novel class of selective Golgi stress inducer (2019)
  • Author(s): Kimura Mayu、Sasaki Kanae、Fukutani Yosuke、Yoshida Hiderou、Ohsawa Ikuroh、Yohda Masafumi、Sakurai Kaori
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 29 Pages: 1732～1736
  • DOI: 10.1016/j.bmcl.2019.05.022
  • Peer Reviewed
• [Journal Article] Golgi stress response and organelle zones (2019)
  • Author(s): Sasaki Kanae、Yoshida Hiderou
  • Journal Title: FEBS Letters Volume: 593 Pages: 2330～2340
  • DOI: 10.1002/1873-3468.13554
  • Peer Reviewed
• [Journal Article] Organelle Zones (2019)
  • Author(s): Sasaki Kanae、Yoshida Hiderou
  • Journal Title: Cell Structure and Function Volume: 44 Pages: 85～94
  • DOI: 10.1247/csf.19010
  • Peer Reviewed
• [Journal Article] Beclin 1 regulates recycling endosome and is required for skin development in mice (2019)
  • Author(s): Noguchi Saori、Honda Shinya、Saitoh Tatsuya、Matsumura Hiroyuki、Nishimura Emi、Akira Shizuo、Shimizu Shigeomi
  • Journal Title: Communications Biology Volume: 2 Pages: 37
  • DOI: 10.1038/s42003-018-0279-0
  • Peer Reviewed
• [Journal Article] Synthesis of Alkynyl Sulfides by Copper-Catalyzed Thiolation of Terminal Alkynes Using Thiosulfonates (2019)
  • Author(s): Kanemoto Kazuya、Yoshida Suguru、Hosoya Takamitsu
  • Journal Title: Organic Letters Volume: 21 Pages: 3172～3177
  • DOI: 10.1021/acs.orglett.9b00875
  • Peer Reviewed
• [Journal Article] Facile Synthesis of Diverse o-Iodoaryl Triflates from o-Silylaryl Triflates by Aluminum-Mediated Desilyliodination. (2019)
  • Author(s): Yoshida S, Hazama Y, Kanemoto K, Nakamura Y, *Hosoya T
  • Journal Title: Chem. Lett Volume: 印刷中 Pages: 印刷中
  • Peer Reviewed
• [Journal Article] Modular Synthesis of Unsymmetrical Doubly-ring-fused Benzene Derivatives Based on a Sequential Ring Construction Strategy Using Oxadiazinones as a Platform Molecule (2019)
  • Author(s): Meguro Tomohiro、Chen Shengnan、Kanemoto Kazuya、Yoshida Suguru、Hosoya Takamitsu
  • Journal Title: Chemistry Letters Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1246/cl.190118
  • Peer Reviewed
• [Journal Article] A facile preparation of functional cycloalkynes via an azide-to-cycloalkyne switching approach (2019)
  • Author(s): Yoshida Suguru、Kuribara Tomoko、Ito Harumi、Meguro Tomohiro、Nishiyama Yoshitake、Karaki Fumika、Hatakeyama Yasutomo、Koike Yuka、Kii Isao、Hosoya Takamitsu
  • Journal Title: Chemical Communications Volume: 55 Pages: 3556～3559
  • DOI: 10.1039/c9cc01113g
  • Peer Reviewed
• [Journal Article] Divergent Synthesis of Photoaffinity Probe Candidates by Click Reactions of Azido-Substituted Aryltrifluoromethyldiazirines (2019)
  • Author(s): Hosoya Takamitsu、Watanabe Kenji、Tsuda Junpei、Ochiai Hidenori、Niwa Takashi
  • Journal Title: HETEROCYCLES Volume: 99 Pages: 1366～1366
  • DOI: 10.3987/COM-18-S(F)76
  • Peer Reviewed
• [Journal Article] Prediction of intracellular targets of a small compound by analyzing peptides presented on MHC class I (2019)
  • Author(s): Sugimoto Yuna、Murohashi Michiko、Arakawa Satoko、Honda Shinya、Shimizu Shigeomi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 508 Pages: 480～486
  • DOI: 10.1016/j.bbrc.2018.11.089
  • Peer Reviewed
• [Journal Article] Role of cyclooxygenase-2-mediated prostaglandin E2-prostaglandin E receptor 4 signaling in cardiac reprogramming (2019)
  • Author(s): Muraoka Naoto、Nara Kaori、Tamura Fumiya、Kojima Hidenori、Yamakawa Hiroyuki、Sadahiro Taketaro、Miyamoto Kazutaka、Isomi Mari、Haginiwa Sho、Tani Hidenori、Kurotsu Shota、Osakabe Rina、Torii Satoru、Shimizu Shigeomi、Okano Hideyuki、Sugimoto Yukihiko、Fukuda Keiichi、Ieda Masaki
  • Journal Title: Nature Communications Volume: 10 Pages: 679
  • DOI: 10.1038/s41467-019-08626-y
  • Peer Reviewed
• [Journal Article] Porin Associates with Tom22 to Regulate the Mitochondrial Protein Gate Assembly (2019)
  • Author(s): Sakaue Haruka、Shiota Takuya、Ishizaka Naoya、Kawano Shin、Tamura Yasushi、Tan Kher Shing、Imai Kenichiro、Motono Chie、Hirokawa Takatsugu、Taki Kentaro、Miyata Non、Kuge Osamu、Lithgow Trevor、Endo Toshiya
  • Journal Title: Molecular Cell Volume: 73 Pages: 1044～1055.e8
  • DOI: 10.1016/j.molcel.2019.01.003
  • Peer Reviewed
• [Journal Article] CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo (2019)
  • Author(s): Sawasato Katsuhiro、Sato Ryo、Nishikawa Hanako、Iimura Naoki、Kamemoto Yuki、Fujikawa Kohki、Yamaguchi Toshiyuki、Kuruma Yutetsu、Tamura Yasushi、Endo Toshiya、Ueda Takuya、Shimamoto Keiko、Nishiyama Ken-ichi
  • Journal Title: Scientific Reports Volume: 9 Pages: 1372
  • DOI: 10.1038/s41598-018-37809-8
  • Peer Reviewed
• [Journal Article] Myristoyl group-aided protein import into the mitochondrial intermembrane space (2019)
  • Author(s): Ueda Eri、Tamura Yasushi、Sakaue Haruka、Kawano Shin、Kakuta Chika、Matsumoto Shunsuke、Endo Toshiya
  • Journal Title: Scientific Reports Volume: 9 Pages: 1185
  • DOI: 10.1038/s41598-018-38016-1
  • Peer Reviewed
• [Journal Article] Maintenance of Cardiolipin and Crista Structure Requires Cooperative Functions of Mitochondrial Dynamics and Phospholipid Transport (2019)
  • Author(s): Kojima Rieko、Kakimoto Yuriko、Furuta Shiina、Itoh Kie、Sesaki Hiromi、Endo Toshiya、Tamura Yasushi
  • Journal Title: Cell Reports Volume: 26 Pages: 518～528.e6
  • DOI: 10.1016/j.celrep.2018.12.070
  • Peer Reviewed
• [Journal Article] PGSE Is a Novel Enhancer Regulating the Proteoglycan Pathway of the Mammalian Golgi Stress Response (2019)
  • Author(s): Sasaki Kanae、Komori Ryota、Taniguchi Mai、Shimaoka Akie、Midori Sachiko、Yamamoto Mayu、Okuda Chiho、Tanaka Ryuya、Sakamoto Miyu、Wakabayashi Sadao、Yoshida Hiderou
  • Journal Title: Cell Structure and Function Volume: 44 Pages: 1～19
  • DOI: 10.1247/csf.18031
  • Peer Reviewed
• [Journal Article] GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain (2019)
  • Author(s): Narentuya、Takeda-Uchimura Yoshiko、Foyez Tahmina、Zhang Zui、Akama Tomoya O.、Yagi Hirokazu、Kato Koichi、Komatsu Yukio、Kadomatsu Kenji、Uchimura Kenji
  • Journal Title: Scientific Reports Volume: 9 Pages: 4387
  • DOI: 10.1038/s41598-019-40901-2
  • Peer Reviewed
• [Journal Article] Generation of the heterogeneity of extracellular vesicles by membrane organization and sorting machineries (2019)
  • Author(s): Harada Yoichiro、Suzuki Takehiro、Fukushige Tomoko、Kizuka Yasuhiko、Yagi Hirokazu、Yamamoto Mika、Kondo Kiyotaka、Inoue Hiromasa、Kato Koichi、Taniguchi Naoyuki、Kanekura Takuro、Dohmae Naoshi、Maruyama Ikuro
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1863 Pages: 681～691
  • DOI: 10.1016/j.bbagen.2019.01.015
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• [Journal Article] Ligand binding to human prostaglandin E receptor EP4 at the lipid-bilayer interface (2018)
  • Author(s): Toyoda Y、Morimoto K、Suno R、Horita S、Yamashita K、Hirata K、Shimizu T、Asada H、Nakane T、Shiimura Y、Nakagita T、Tsuge K、Yoshida S、Kuribara Tomoko、Hosoya Takamitsu、Sugimoto Y、Nomura N、Sato M、Hirokawa T、Kinoshita M、Murata T、Takayama K、Yamamoto M、Narumiya S、Iwata S、Kobayashi T
  • Journal Title: Nature Chemical Biology Volume: 15 Pages: 18～26
  • DOI: 10.1038/s41589-018-0131-3
  • Peer Reviewed
• [Journal Article] Association between aromatase in human brains and personality traits (2018)
  • Author(s): Takahashi Kayo、Hosoya Takamitsu、Onoe Kayo、Takashima Tadayuki、Tanaka Masaaki、Ishii Akira、Nakatomi Yasuhito、Tazawa Shusaku、Takahashi Kazuhiro、Doi Hisashi、Wada Yasuhiro、Watanabe Yasuyoshi
  • Journal Title: Scientific Reports Volume: 8 Pages: 16841
  • DOI: 10.1038/s41598-018-35065-4
  • Peer Reviewed
• [Journal Article] Further enhancement of the clickability of doubly sterically-hindered aryl azides by para-amino substitution (2018)
  • Author(s): Yoshida Suguru、Tanaka Junko、Nishiyama Yoshitake、Hazama Yuki、Matsushita Takeshi、Hosoya Takamitsu
  • Journal Title: Chemical Communications Volume: 54 Pages: 13499～13502
  • DOI: 10.1039/c8cc05791e
  • Peer Reviewed
• [Journal Article] Palladium(ii)-mediated rapid 11C-cyanation of (hetero)arylborons (2018)
  • Author(s): Zhang Zhouen、Niwa Takashi、Watanabe Yasuyoshi、Hosoya Takamitsu
  • Journal Title: Organic & Biomolecular Chemistry Volume: 16 Pages: 7711～7716
  • DOI: 10.1039/c8ob02049c
  • Peer Reviewed
• [Journal Article] Hydrosilyl Group-directed Iridium-catalyzed peri-Selective C?H Borylation of Ring-fused (Hetero)Arenes (2018)
  • Author(s): Sumida Yuto、Harada Ryu、Sumida Tomoe、Hashizume Daisuke、Hosoya Takamitsu
  • Journal Title: Chemistry Letters Volume: 47 Pages: 1251～1254
  • DOI: 10.1246/cl.180594
  • Peer Reviewed
• [Journal Article] Generation of Arynes by Selective Cleavage of a Carbon?Phosphorus Bond of o-(Diarylphosphinyl)aryl Triflates Using a Grignard Reagent (2018)
  • Author(s): Nishiyama Yoshitake、Kamada Shuhei、Yoshida Suguru、Hosoya Takamitsu
  • Journal Title: Chemistry Letters Volume: 47 Pages: 1216～1219
  • DOI: 10.1246/cl.180555
  • Peer Reviewed
• [Journal Article] Transient Protection of Organic Azides from Click Reactions with Alkynes by Phosphazide Formation (2018)
  • Author(s): Meguro Tomohiro、Yoshida Suguru、Igawa Kazunobu、Tomooka Katsuhiko、Hosoya Takamitsu
  • Journal Title: Organic Letters Volume: 20 Pages: 4126～4130
  • DOI: 10.1021/acs.orglett.8b01692
  • Peer Reviewed
• [Journal Article] Expanding the synthesizable multisubstituted benzo[b]thiophenes via 6,7-thienobenzynes generated from o-silylaryl triflate-type precursors (2018)
  • Author(s): Yoshida Suguru、Kuribara Tomoko、Morita Takamoto、Matsuzawa Tsubasa、Morimoto Kazushi、Kobayashi Takuya、Hosoya Takamitsu
  • Journal Title: RSC Advances Volume: 8 Pages: 21754～21758
  • DOI: 10.1039/C8RA04035D
  • Peer Reviewed
• [Journal Article] Staudinger reaction using 2,6-dichlorophenyl azide derivatives for robust aza-ylide formation applicable to bioconjugation in living cells (2018)
  • Author(s): Meguro Tomohiro、Terashima Norikazu、Ito Harumi、Koike Yuka、Kii Isao、Yoshida Suguru、Hosoya Takamitsu
  • Journal Title: Chemical Communications Volume: 54 Pages: 7904～7907
  • DOI: 10.1039/C8CC00179K
  • Peer Reviewed
• [Journal Article] Slow luminescence kinetics of semi-synthetic aequorin: expression, purification and structure determination of cf3-aequorin (2018)
  • Author(s): Inouye Satoshi、Tomabechi Yuri、Hosoya Takamitsu、Sekine Shun-ichi、Shirouzu Mikako
  • Journal Title: The Journal of Biochemistry Volume: 164 Pages: 247～255
  • DOI: 10.1093/jb/mvy049
  • Peer Reviewed
• [Journal Article] CDK9 Inhibitor FIT-039 Suppresses Viral Oncogenes E6 and E7 and Has a Therapeutic Effect on HPV-Induced Neoplasia (2018)
  • Author(s): Ajiro Masahiko、Sakai Hiroyuki、Onogi Hiroshi、Yamamoto Makoto、Sumi Eriko、Sawada Teruo、Nomura Takashi、Kabashima Kenji、Hosoya Takamitsu、Hagiwara Masatoshi
  • Journal Title: Clinical Cancer Research Volume: 24 Pages: 4518～4528
  • DOI: 10.1158/1078-0432.CCR-17-3119
  • Peer Reviewed
• [Journal Article] Synthesis of Diverse Phenothiazines by Direct Thioamination of Arynes with S-(o-Bromoaryl)-S-methylsulfilimines and Subsequent Intramolecular Buchwald?Hartwig Amination (2018)
  • Author(s): Matsuzawa Tsubasa、Uchida Keisuke、Yoshida Suguru、Hosoya Takamitsu
  • Journal Title: Chemistry Letters Volume: 47 Pages: 825～828
  • DOI: 10.1246/cl.180304
  • Peer Reviewed
• [Journal Article] Quantification of receptor activation by oxytocin and vasopressin in endocytosis-coupled bioluminescence reduction assay using nanoKAZ (2018)
  • Author(s): Kii Isao、Hirahara-Owada Shino、Yamaguchi Masataka、Niwa Takashi、Koike Yuka、Sonamoto Rie、Ito Harumi、Takahashi Kayo、Yokoyama Chihiro、Hayashi Takuya、Hosoya Takamitsu、Watanabe Yasuyoshi
  • Journal Title: Analytical Biochemistry Volume: 549 Pages: 174～183
  • DOI: 10.1016/j.ab.2018.04.001
  • Peer Reviewed
• [Journal Article] Convergent synthesis of trifunctional molecules by three sequential azido-type-selective cycloadditions (2018)
  • Author(s): Yoshida Suguru、Kanno Kimiyuki、Kii Isao、Misawa Yoshihiro、Hagiwara Masatoshi、Hosoya Takamitsu
  • Journal Title: Chemical Communications Volume: 54 Pages: 3705～3708
  • DOI: 10.1039/c8cc01195h
  • Peer Reviewed
• [Journal Article] Association Between Autophagy and Neurodegenerative Diseases (2018)
  • Author(s): Fujikake Nobuhiro、Shin Minkyoung、Shimizu Shigeomi
  • Journal Title: Frontiers in Neuroscience Volume: 12 Pages: 255
  • DOI: 10.3389/fnins.2018.00255
  • Peer Reviewed
• [Journal Article] Organelle zones in mitochondria (2018)
  • Author(s): Shimizu Shigeomi
  • Journal Title: The Journal of Biochemistry Volume: 165 Pages: 101～107
  • DOI: 10.1093/jb/mvy068
  • Peer Reviewed
• [Journal Article] Discovery and Optimization of Inhibitors of the Parkinson’s Disease Associated Protein DJ-1 (2018)
  • Author(s): Tashiro Shinya、Caaveiro Jose M. M.、Nakakido Makoto、Tanabe Aki、Nagatoishi Satoru、Tamura Yasushi、Matsuda Noriyuki、Liu Dali、Hoang Quyen Q.、Tsumoto Kouhei
  • Journal Title: ACS Chemical Biology Volume: 13 Pages: 2783～2793
  • DOI: 10.1021/acschembio.8b00701
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system (2018)
  • Author(s): Kakimoto Yuriko、Tashiro Shinya、Kojima Rieko、Morozumi Yuki、Endo Toshiya、Tamura Yasushi
  • Journal Title: Scientific Reports Volume: 8 Pages: 6175
  • DOI: 10.1038/s41598-018-24466-0
  • Peer Reviewed
• [Journal Article] Structural Biological Approach to Biopharmaceuticals (2018)
  • Author(s): Kato Koichi、Yanaka Saeko、Yagi Hirokazu
  • Journal Title: YAKUGAKU ZASSHI Volume: 138 Pages: 1495～1502
  • DOI: 10.1248/yakushi.18-00020-3
  • Peer Reviewed
• [Journal Article] Structure and Dynamics of Immunoglobulin G Glycoproteins (2018)
  • Author(s): Yagi Hirokazu、Yanaka Saeko、Kato Koichi
  • Journal Title: Adv. Exp. Med. Biol. Volume: 1104 Pages: 219～235
  • DOI: 10.1007/978-981-13-2158-0_11
  • Peer Reviewed
• [Journal Article] Distinct substrate specificities of human GlcNAc-6-sulfotransferases revealed by mass spectrometry?based sulfoglycomic analysis (2018)
  • Author(s): Yu Shin-Yi、Hsiao Cheng-Te、Izawa Mineko、Yusa Akiko、Ishida Hiroji、Nakamura Shigeo、Yagi Hirokazu、Kannagi Reiji、Khoo Kay-Hooi
  • Journal Title: Journal of Biological Chemistry Volume: 293 Pages: 15163～15177
  • DOI: 10.1074/jbc.RA118.001937
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] N-glycan structures of human alveoli provide insight into influenza A virus infection and pathogenesis (2018)
  • Author(s): Sriwilaijaroen Nongluk、Nakakita Shin-ichi、Kondo Sachiko、Yagi Hirokazu、Kato Koichi、Murata Takeomi、Hiramatsu Hiroaki、Kawahara Toshio、Watanabe Yohei、Kanai Yasushi、Ono Takao、Hirabayashi Jun、Matsumoto Kazuhiko、Suzuki Yasuo
  • Journal Title: The FEBS Journal Volume: 285 Pages: 1611～1634
  • DOI: 10.1111/febs.14431
  • Peer Reviewed
• [Journal Article] Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems (2018)
  • Author(s): Yanaka Saeko、Yagi Hirokazu、Yogo Rina、Yagi-Utsumi Maho、Kato Koichi
  • Journal Title: Journal of Biomolecular NMR Volume: 71 Pages: 193～202
  • DOI: 10.1007/s10858-018-0169-2
  • Peer Reviewed
• [Journal Article] Dram1 regulates DNA damage-induced alternative autophagy (2018)
  • Author(s): Nagata Meruna、Arakawa Satoko、Yamaguchi Hirofumi、Torii Satoru、Endo Hazuki、Tsujioka Masatsune、Honda Shinya、Nishida Yuya、Konishi Akimitsu、Shimizu Shigeomi
  • Journal Title: Cell Stress Volume: 2 Pages: 55～65
  • DOI: 10.15698/cst2018.03.127
  • Peer Reviewed / Open Access
• [Journal Article] Small fluorescent molecules for monitoring autophagic flux (2018)
  • Author(s): Iwashita Hidefumi、Sakurai Hajime Tajima、Nagahora Noriyoshi、Ishiyama Munetaka、Shioji Kosei、Sasamoto Kazumi、Okuma Kentaro、Shimizu Shigeomi、Ueno Yuichiro
  • Journal Title: FEBS Letters Volume: 592 Pages: 559～567
  • DOI: 10.1002/1873-3468.12979
  • Peer Reviewed
• [Journal Article] Biological Roles of Alternative Autophagy (2018)
  • Author(s): Shigeomi Shimizu
  • Journal Title: Mol. Cells Volume: 41 Pages: 50-54
  • DOI: 10.14348/molcells.2018.2215
  • Peer Reviewed
• [Journal Article] Site-specific N-glycosylation analysis of soluble Fcγ receptor IIIb in human serum (2018)
  • Author(s): Yagi Hirokazu、Takakura Daisuke、Roumenina Lubka T.、Fridman Wolf Herman、Saut?s-Fridman Catherine、Kawasaki Nana、Kato Koichi
  • Journal Title: Scientific Reports Volume: 8 Pages: 2719
  • DOI: 10.1038/s41598-018-21145-y
  • Peer Reviewed / Open Access
• [Journal Article] Role of Atg5-dependent cell death in the embryonic development of Bax/Bak double-knockout mice (2017)
  • Author(s): Arakawa Satoko、Tsujioka Masatsune、Yoshida Tatsushi、Tajima-Sakurai Hajime、Nishida Yuya、Matsuoka Yosuke、Yoshino Ikuyo、Tsujimoto Yoshihide、Shimizu Shigeomi
  • Journal Title: Cell Death and Differentiation Volume: 24 Pages: 1598～1608
  • DOI: 10.1038/cdd.2017.84
  • Peer Reviewed
• [Journal Article] Structure-function insights into direct lipid transfer between membranes by Mmm1-Mdm12 of ERMES (2017)
  • Author(s): Kawano Shin、Tamura Yasushi、Kojima Rieko、Bala Siqin、Asai Eri、Michel Agnes H.、Kornmann Benoit、Riezman Isabelle、Riezman Howard、Sakae Yoshitake、Okamoto Yuko、Endo Toshiya
  • Journal Title: The Journal of Cell Biology Volume: 217 Pages: 959～974
  • DOI: 10.1083/jcb.201704119
  • Peer Reviewed
• [Journal Article] Role of Intra- and Inter-mitochondrial Membrane Contact Sites in Yeast Phospholipid Biogenesis (2017)
  • Author(s): Tamura Yasushi、Endo Toshiya
  • Journal Title: Adv Exp Med Biol. Volume: 997 Pages: 121～133
  • DOI: 10.1007/978-981-10-4567-7_9
  • Peer Reviewed
• [Journal Article] Lewis X-Carrying Neoglycolipids Evoke Selective Apoptosis in Neural Stem Cells (2017)
  • Author(s): Yagi Hirokazu、Yan Gengwei、Suzuki Tatsuya、Tsuge Shingo、Yamaguchi Takumi、Kato Koichi
  • Journal Title: Neurochemical Research Volume: 43 Pages: 212～218
  • DOI: 10.1007/s11064-017-2415-5
  • Peer Reviewed
• [Presentation] Roles of phospholipid transport in mitochondrial biogenesis (2019)
  • Author(s): Yasushi Tamura
  • Organizer: The International Conference on the Bioscience of Lipids (AMED-Lipid Satellite Symposium)
  • Int'l Joint Research / Invited
• [Presentation] Identification of novel factors involved in formation of ER-mitochondria contact sites (2019)
  • Author(s): Yasushi Tamura
  • Organizer: Organelle zones: opening a new era of Cell Biology
  • Int'l Joint Research / Invited
• [Presentation] Regulatory mechanisms controlling the number of ER-mitochondria contact sites (2019)
  • Author(s): Yasushi Tamura
  • Organizer: Institute of Biochemistry and Molecular Biology, Freiburg University, Research seminar
  • Int'l Joint Research / Invited
• [Presentation] Two independent regulatory mechanisms controlling the number of ER-mitochondria contact sites (2019)
  • Author(s): Yasushi Tamura
  • Organizer: Organelle zones meet compartmental gates and contact sites
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms and physiological roles of alternative autophagy (2019)
  • Author(s): Shigeomi Shimizu
  • Organizer: The 92nd Annual Meeting of the Japanese Biochemical Society
• [Presentation] Molecular mechanisms and physiological roles of Atg5-independent mitophagy (2019)
  • Author(s): Shigeomi Shimizu
  • Organizer: ASMRM & J-mit
  • Int'l Joint Research / Invited
• [Presentation] New insights into the mechanism of Golgi-mediated alternative autophagy using chemical biology approach (2019)
  • Author(s): Shigeomi Shimizu
  • Organizer: International Symposium of Autophagy
  • Int'l Joint Research / Invited
• [Presentation] Promotion of α2,3-sialylation of recombinant erythropoietin by tagging it with a 10-aminoacid sequence recognized by a cargo receptor complex (2019)
  • Author(s): Hirokazu Yagi, Rena Honda, Rino Yamada, Maho Yagi-Utsumi1, Kazuhiro Aoki, and Koichi Kato
  • Organizer: 25th International Symposium on Glycoconjugates
  • Int'l Joint Research
• [Presentation] Deciphering the molecular code for FUT9-dependent Lewis X modification (2019)
  • Author(s): Taiki Saito, Hirokazu Yagi, Chuwei Kuo, Kay-Hooi Khoo, and Koichi Kato
  • Organizer: 25th International Symposium on Glycoconjugates
  • Int'l Joint Research
• [Presentation] オートファジーと疾患 (2018)
  • Author(s): 清水重臣
  • Organizer: 第6回AAA会
  • Invited
• [Presentation] オートファジーが関わる細胞死 (2017)
  • Author(s): 清水重臣
  • Organizer: 日本Cell Death学会
  • Invited
• [Presentation] 新規オートファジーの分子機構とその破綻による疾患 (2017)
  • Author(s): 清水重臣
  • Organizer: 第22回 日本病態プロテアーゼ学会
  • Invited
• [Presentation] Development of molecularly targeted anticancer agents based on the regulation of autophagic cell death (2017)
  • Author(s): 清水重臣
  • Organizer: 第76回日本癌学会
  • Invited
• [Presentation] Structural and Mechanistic Insights into Phospholipid Transfer via Mitochondria (2017)
  • Author(s): Yasushi Tamura
  • Organizer: 5th International Conference on Smart Systems Engineering
  • Int'l Joint Research
• [Presentation] The characterization of the laminin-binding glycans on α-dystroglycan catalyzed by several causative gene products of dystroglycanopathy (2017)
  • Author(s): Hirokazy Yagi
  • Organizer: 1st International Conference on the Glycobiology of Nervous System
  • Int'l Joint Research / Invited
• [Remarks] 東京医科歯科大学 病態細胞生物ホームページ
  • URL: http://www.tmd.ac.jp/mri/pcb/index.html
• [Remarks] オルガネラゾーン ホームページ
  • URL: http://organellezone.org/
• [Remarks] 東京医科歯科大学病態細胞生物ホームページ
  • URL: http://www.tmd.ac.jp/mri/pcb/index.html
• [Remarks] 東京医科歯科大学生命有機化学ホームページ
  • URL: http://chembiolab.sakura.ne.jp/Home.html
• [Remarks] 山形大学理学部・田村研究室ホームページ
  • URL: https://www.tamuralab.com/
• [Remarks] 兵庫県立大学大学院生命理学研究科ホームページ
  • URL: http://www.eonet.ne.jp/~biophysics-kyoto/
• [Remarks] 名古屋市立大学 大学院薬学研究科 生命構造学分野ホームページ
  • URL: http://www.phar.nagoya-cu.ac.jp/hp/sbk/
• [Patent(Industrial Property Rights)] 糖タンパク質の糖修飾 (2018)
  • Inventor(s): 矢木宏和、齋藤泰輝、加藤晃一
  • Industrial Property Rights Holder: 矢木宏和、齋藤泰輝、加藤晃一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2018-047235
• [Patent(Industrial Property Rights)] 糖タンパク質の生産方法 (2017)
  • Inventor(s): 矢木宏和、本田怜奈、加藤晃一
  • Industrial Property Rights Holder: 矢木宏和、本田怜奈、加藤晃一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2017-132312