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高分解能・時間分解構造解析による水分解反応の機構解明

Planned

Project AreaCreation of novel light energy conversion system through elucidation of the molecular mechanism of photosynthesis and its artificial design in terms of time and space
Project/Area Number 17H06434
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Complex systems
Research InstitutionOkayama University

Principal Investigator

沈 建仁  岡山大学, 異分野基礎科学研究所, 教授 (60261161)

Co-Investigator(Kenkyū-buntansha) 神谷 信夫  大阪市立大学, 人工光合成研究センター, 名誉教授 (60152865)
山口 兆  大阪大学, 産業科学研究所, 特任教授 (80029537)
Project Period (FY) 2017-06-30 – 2022-03-31
Project Status Granted (Fiscal Year 2020)
Budget Amount *help
¥310,960,000 (Direct Cost: ¥239,200,000、Indirect Cost: ¥71,760,000)
Fiscal Year 2020: ¥51,090,000 (Direct Cost: ¥39,300,000、Indirect Cost: ¥11,790,000)
Fiscal Year 2019: ¥51,090,000 (Direct Cost: ¥39,300,000、Indirect Cost: ¥11,790,000)
Fiscal Year 2018: ¥51,090,000 (Direct Cost: ¥39,300,000、Indirect Cost: ¥11,790,000)
Fiscal Year 2017: ¥106,600,000 (Direct Cost: ¥82,000,000、Indirect Cost: ¥24,600,000)
Keywords光合成 / 水分解反応 / X線結晶構造解析 / 理論計算 / X線自由電子レーザー / 動的構造解析 / 酸素発生反応 / 時間分解構造解析 / 密度行列繰り込み群法(DMRG) / 生物物理 / タンパク質
Outline of Annual Research Achievements

フェムト秒のX線自由電子レーザー(XFEL)を用いたポンプープローブシリアルフェムト秒結晶構造解析法により、光化学系II (PSII) 微結晶について2閃光照射後の遅延時間がナノ秒からマイクロ秒にわたる複数の時点でX線回折データを収集し、構造解析を行った。その結果、光誘導による電子伝達・プロトン移動に伴うPSIIの構造変化を捕えた。また、暗条件で得られたPSIIの結晶について、同型性の高い試料を多数調製する脱水・クライオ処理条件を確立し、0.1 MGy以下の低X線照射量で回折強度を測定することで、X線によるMnの還元を回避した。その条件下で複数セットのX線回折データを収集し、構造解析を行った結果、結合距離誤差の見積もりを0.05 A 以下まで低減させたMn4CaO5クラスターの精密構造を得ることに成功し、従来より信頼度の高い構造学的知見を得ることができた。

これまで使用されていた密度汎関数(DFT)法を凌駕する高精度理論化学計算法、密度行列繰り込み群(Density Matrix Renormalized Group;DMRG)と結合クラスター(Coupled-Cluster; CC)をMn錯体に適用し、異なるリガンドとスピン状態にある一核と二核Mnの相対安定性を評価した。これらの結果をもとに、PSIIなどの高核Mn錯体のEPR実験結果の理論的解析法の導出に成功した。

上記研究と並行して、PSIIの進化的祖先である酸素非発生型硫黄細菌由来反応中心(RC)-光捕集アンテナ(LHCI)超分子複合体の結晶構造を高分解能で解析し、論文をNature誌に公表した。また、構造未知の、珪藻由来PSIIの光捕集アンテナタンパク質であるクロロフィルc-フコキサンチン結合蛋白質FCPの構造を高分解能で解析し、成果をScience誌に公表した。

Current Status of Research Progress
Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

X線自由電子レーザーを用いたポンプープローブ時間分解構造解析法をPSII微結晶に適用し、光照射により誘導させる微小な構造変化を検出するとともに、極めて低X線照射量と高分解能でPSIIの構造を解析し、Mn4CaO5のより精密な構造を解析した。また、従来の(DFT)法を超える高精度理論化学計算法、DMRG法をPSIIの理論計算に適用し、Mn化合物の相対安定性について重要な知見を得た。合わせて、PSIIの進化的祖先である酸素非発生型硫黄細菌由来の巨大膜タンパク質複合体、RC- LHCI複合体 (サブユニット数36個、総分子量400 kDa)の構造を1.9 A分解能で解析し、論文をNature誌に公表し、珪藻由来PSIIの光捕集アンテナタンパク質FCPの構造を1.8 A分解能で解析し、論文をScience誌に公表した。これらの成果から、研究が当初の計画以上に進展していると判断した。

Strategy for Future Research Activity

これまで構造変化が検出されていない、3閃光照射によるPSIIのS状態遷移に伴う構造変化 (S3→(S4)→S0)を検出するため、PSII微結晶の溶液条件を改善し、S3状態以降の遷移効率が十分高い微結晶の条件を確立し、XFELを利用したポンプープローブSFX法で S3→(S4)→S0遷移に伴う構造変化を解析する。同時に、1閃光、2閃光照射後の異なる遅延時間で複数時点でのX線回折データを収集し、光誘導による電子伝達・プロトン移動に伴うPSIIの構造変化をより詳細に解析する。また、暗順応状態にあるPSII結晶について、pHに依存した構造の変化を高分解能で追跡し、アミノ酸のプロトン化・脱プロトン化によるPSII構造と機能への影響を明らかにする。さらにヨウ素イオンやヒドロキシルアミンなどにより還元されたPSIIの構造を解析し、S0状態またはそれより進んだ還元状態にあるPSIIの構造を解明する。
以上より得られた各状態のPSIIの実験構造について、DFT法を超えるDMRG法を用いて解析し、実験結果と合わせてPSIIの詳細な構造と反応機構を解明する。

Report

(2 results)
  • 2018 Annual Research Report
  • 2017 Annual Research Report

Research Products

(90 results)

All 2019 2018 2017 Other

All Int'l Joint Research Journal Article Presentation Book Remarks

  • [Int'l Joint Research] University of Colorado, Boulder(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 中国科学院植物研究所/中国科学院大連化学物理研究所(中国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] UMR CNRS 9198, CEA Saclay(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Australia National University(オーストラリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Hungarian Academy of Sciences(ハンガリー)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 中国科学院植物研究所/中国科学院化学研究所(中国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] オーストラリア国立大学(オーストラリア)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Properties and structure of a low-potential, penta-heme cytochrome c552 from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium tepidum2019

    • Author(s)
      Chen J.-H., Yu L.-J., Boussac A., Wang-Otomo Z.-Y., Kuang T., Shen J.-R.
    • Journal Title

      Photosynthesis Research

      Volume: 139 Pages: 281-293

    • DOI

      10.1007/s11120-018-0507-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Site-directed mutagenesis of two amino acid residues in cytochrome b559 α subunit that interact with a phosphatidylglycerol molecule (PG772) induces quinone-dependent inhibition of photosystem II activity2019

    • Author(s)
      Endo Kaichiro、Kobayashi Koichi、Wang Hsing-Ting、Chu Hsiu-An、Shen Jian-Ren、Wada Hajime
    • Journal Title

      Photosynthesis Research

      Volume: 139 Pages: 267-279

    • DOI

      10.1007/s11120-018-0555-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Low-Energy Chlorophylls in Fucoxanthin Chlorophyll a/c-Binding Protein Conduct Excitation Energy Transfer to Photosystem I in Diatoms2019

    • Author(s)
      Nagao, R., Yokono, M., Ueno, Y., Shen, J.-R., Akimoto, S.
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 123 Pages: 66-70

    • DOI

      10.1021/acs.jpcb.8b09253

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of a green algal photosystem I in complex with a large number of light-harvesting complex I subunits2019

    • Author(s)
      Qin Xiaochun、Pi Xiong、Wang Wenda、Han Guangye、Zhu Lixia、Liu Mingmei、Cheng Linpeng、Shen Jian-Ren、Kuang Tingyun、Sui Sen-Fang
    • Journal Title

      Nature Plants

      Volume: 5 Pages: 263-272

    • DOI

      10.1038/s41477-019-0379-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Structural baisis for blue-green light harvesting and energy dissipation in diatoms2019

    • Author(s)
      Wang Wenda、Yu Long-Jiang、Xu Caizhe、Tomizaki Takashi、Zhao Songhao、Umena Yasufumi、Chen Xiaobo、Qin Xiaochun、Xin Yueyong、Suga Michihiro、Han Guangye、Kuang Tingyun、Shen Jian-Ren
    • Journal Title

      Science

      Volume: 363

    • DOI

      10.1126/science.aav0365

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cobalt/Cobalt Oxide Surface for Water Oxidation2019

    • Author(s)
      Feizi Hadi、Bagheri Robabeh、Song Zhenlun、Shen Jian-Ren、Allakhverdiev Suleyman I.、Najafpour Mohammad Mahdi
    • Journal Title

      ACS Sustainable Chemistry & Engineering

      Volume: 7 Pages: 6093-6105

    • DOI

      10.1021/acssuschemeng.8b06269

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Redox transients of P680 associated with the incremental chlorophyll‐ a fluorescence yield rises elicited by a series of saturating flashes in diuron‐treated photosystem II core complex of Thermosynechococcus vulcanus2019

    • Author(s)
      Sipka Gabor、Muller Pavel、Brettel Klaus、Magyar Melinda、Kovacs Laszlo、Zhu Qingjun、Xiao Yanan、Han Guangye、Lambrev Petar H.、Shen Jian‐Ren、Garab Gyozo
    • Journal Title

      Physiologia Plantarum

      Volume: 166 Pages: 22-32

    • DOI

      10.1111/ppl.12945

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ultrafast Excitation Energy Dynamics in a Diatom Photosystem I-Antenna Complex: A Femtosecond Fluorescence Upconversion Study2019

    • Author(s)
      Nagao, R., Kagatani, K., Ueno, Y., Shen, J.-R., Akimoto, S.
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 123 Pages: 2673-2678

    • DOI

      10.1021/acs.jpcb.8b12086

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] UNO DMRG CAS CI calculations of binuclear manganese complex Mn(IV)2O2(NHCHCO2)4: Scope and applicability of Heisenberg model2019

    • Author(s)
      Kawakami Takashi、Miyagawa Koichi、Sharma Sandeep、Saito Toru、Shoji Mitsuo、Yamada Satoru、Yamanaka Shusuke、Okumura Mitsutaka、Nakajima Takahito、Yamaguchi Kizashi
    • Journal Title

      J. Comput. Chem.

      Volume: 40 Pages: 333-341

    • DOI

      10.1002/jcc.25602

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Concerted bond switching mechanism coupled with one-electron transfer for the oxygen-oxygen bond formation in the oxygen-evolving complex of photosystem II2019

    • Author(s)
      Shoji Mitsuo、Isobe Hiroshi、Yamaguchi Kizashi
    • Journal Title

      Chemical Physics Letters

      Volume: 714 Pages: 219-226

    • DOI

      10.1016/j.cplett.2018.10.041

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possibility of the right-opened Mn-oxo intermediate (R-oxo (4444)) among all nine intermediates in the S3 state of the oxygen-evolving complex of photosystem II revealed by large-scale QM/MM calculations2019

    • Author(s)
      Shoji Mitsuo、Isobe Hiroshi、Miyagawa Koichi、Yamaguchi Kizashi
    • Journal Title

      Chemical Physics Letters

      Volume: 518 Pages: 81-90

    • DOI

      10.1016/j.chemphys.2018.11.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Theoretical and computational investigations of geometrical, electronic and spin structures of the CaMn4OX (X=5, 6) cluster in the Kok cycle Si (i=0-3) of oxygen evolving complex of photosystem II2019

    • Author(s)
      Yamaguchi Kizashi、Yamanaka Shusuke、Isobe Hiroshi、Shoji Mitsuo、Miyagawa Kouichi、Nakajima Takahito、Kawakami Takashi、Okumura Mitsutaka
    • Journal Title

      Physiologia Plantarum

      Volume: 166 Pages: 44-59

    • DOI

      10.1111/ppl.12960

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spin, Valence, and Structural Isomerism in the S3 State of the Oxygen-Evolving Complex of Photosystem II as a Manifestation of Multimetallic Cooperativity2019

    • Author(s)
      Isobe Hiroshi、Shoji Mitsuo、Suzuki Takayoshi、Shen Jian-Ren、Yamaguchi Kizashi
    • Journal Title

      Journal of Chemical Theory and Computation

      Volume: 15 Pages: 2375-2391

    • DOI

      10.1021/acs.jctc.8b01055

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Linear Response Functions of Densities and Spin Densities for Systematic Modeling of the QM/MM Approach for Mono- and Poly-Nuclear Transition Metal Systems2019

    • Author(s)
      Kitakawa Colin、Maruyama Tomohiro、Oonari Jinta、Mitsuta Yuki、Kawakami Takashi、Okumura Mitsutaka、Yamaguchi Kizashi、Yamanaka Shusuke
    • Journal Title

      Molecules

      Volume: 24 Pages: 821-821

    • DOI

      10.3390/molecules24040821

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Theoretical Elucidation of Geometrical Structures of the CaMn4O5 Cluster in Oxygen Evolving Complex of Photosystem II Scope and Applicability of Estimation Formulae of Structural Deformations via the Mixed-Valence and Jahn?Teller Effects2019

    • Author(s)
      Shoji Mitsuo、Isobe Hiroshi、Yamanaka Shusuke、Umena Yasufumi、Kawakami Keisuke、Kamiya Nobuo、Yamaguchi Kizashi
    • Journal Title

      Adv. Q. Chem.

      Volume: 78 Pages: 307-451

    • DOI

      10.1016/bs.aiq.2018.05.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of photosynthetic LH1?RC supercomplex at 1.9A resolution2018

    • Author(s)
      Yu Long-Jiang、Suga Michihiro、Wang-Otomo Zheng-Yu、Shen Jian-Ren
    • Journal Title

      Nature

      Volume: 556 Pages: 209-213

    • DOI

      10.1038/s41586-018-0002-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A siphonous morphology affects light-harvesting modulation in the intertidal green macroalga Bryopsis corticulans (Ulvophyceae)2018

    • Author(s)
      Giovagnetti Vasco、Han Guangye、Ware Maxwell A.、Ungerer Petra、Qin Xiaochun、Wang Wen-Da、Kuang Tingyun、Shen Jian-Ren、Ruban Alexander V.
    • Journal Title

      Planta

      Volume: 247 Pages: 1293-1306

    • DOI

      10.1007/s00425-018-2854-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Unique organization of photosystem I-light harvesting supercomplex revealed by cryo-EM from a red alga2018

    • Author(s)
      Pi X., Tian L., Dai H.-E., Qin X., Cheng L., Kuang T., Sui S.-F., Shen J.-R.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 115 Pages: 4423-4428

    • DOI

      10.1073/pnas.1722482115

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fourier transform infrared analysis of the S-state cycle of water oxidation in the microcrystals of photosystem II.2018

    • Author(s)
      Kato Yuki、Akita Fusamichi、Nakajima Yoshiki、Suga Michihiro、Umena Yasufumi、Shen Jian-Ren、Noguchi Takumi
    • Journal Title

      J. Phys. Chem. Lett.

      Volume: 9 Pages: 2121-2126

    • DOI

      10.1021/acs.jpclett.8b00638

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alterations of pigment composition and their interactions in response to different light conditions in the diatom Chaetoceros gracilis probed by time-resolved fluorescence spectroscopy2018

    • Author(s)
      Nagao, R., Ueno, Y., Yokono, M., Shen, J.-R., Akimoto, S.
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Bioenergetics

      Volume: 1859 Pages: 524-530

    • DOI

      10.1016/j.bbabio.2018.04.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biohybrid solar cells: Fundamentals, progress, and challenges2018

    • Author(s)
      Musazade E., Voloshin R., Brady N., Mondal J., Atashova S., Zharmukhamedov S. K., Huseynova I., Ramakrishna S., Najafpour M. M., Shen J.-R., Bruce B. D., Allakhverdiev S. I.
    • Journal Title

      Journal of Photochemistry and Photobiology C: Photochemistry Reviews

      Volume: 35 Pages: 134-156

    • DOI

      10.1016/j.jphotochemrev.2018.04.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Thylakoid membrane lipid sulfoquinovosyl-diacylglycerol (SQDG) is required for full functioning of photosystem II in Thermosynechococcus elongatus.2018

    • Author(s)
      Nakajima, Y., Umena, Y., Nagao, R., Endo, K., Kobayashi, K., Akita, F., Suga, M., Wada, H., Noguchi, T., Shen, J.-R.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 293 Pages: 14786-14797

    • DOI

      10.1074/jbc.ra118.004304

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel features of LH1-RC from Thermochromatium tepidum revealed from its atomic resolution structure2018

    • Author(s)
      Yu Long-Jiang、Suga Michihiro、Wang-Otomo Zheng-Yu、Shen Jian-Ren
    • Journal Title

      The FEBS Journal

      Volume: 285 Pages: 4359-4366

    • DOI

      10.1111/febs.14679

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A transparent electrode with water-oxidizing activity2018

    • Author(s)
      Azadi G., Bagheri R., Bikas R., Mousazade Y., Cui J., Song Z., Kinzhybalo V., Shen J.-R., Allakhverdiev S. I., Najafpour M. M.
    • Journal Title

      International Journal of Hydrogen Energy

      Volume: 43 Pages: 22896-22904

    • DOI

      10.1016/j.ijhydene.2018.10.146

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biochemical characterization of photosystem I complexes having different subunit compositions of fucoxanthin chlorophyll a/c-binding proteins in the diatom Chaetoceros gracilis2018

    • Author(s)
      Nagao, R., Ueno, Y., Akita, F., Suzuki, T., Dohmae, N., Akimoto, S., Shen, J.-R.
    • Journal Title

      Photosynthesis Research

      Volume: 印刷中 Pages: 141-149

    • DOI

      10.1007/s11120-018-0576-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Understanding Two Different Structures in the Dark Stable State of the Oxygen-Evolving Complex of Photosystem II: Applicability of the Jahn-Teller Deformation Formula2018

    • Author(s)
      Shoji Mitsuo、Isobe Hiroshi、Tanaka Ayako、Fukushima Yoshimasa、Kawakami Keisuke、Umena Yasufumi、Kamiya Nobuo、Nakajima Takahito、Yamaguchi Kizashi
    • Journal Title

      ChemPhotoChem

      Volume: 2 Pages: 257-270

    • DOI

      10.1002/cptc.201700162

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relative stability between the manganese hydroxide- and oxo-models for water oxidation by CCSD, DMRG CASCI, CASSCF, CASPT2 and CASDFT methods; Importance of static and dynamical electron correlation effects for OEC of PSII2018

    • Author(s)
      Kawakami T.、Miyagawa K.、Isobe H.、Shoji M.、Yamanaka S.、Katouda M.、Nakajima T.、Nakatani K.、Okumura M.、Yamaguchi K.
    • Journal Title

      Chemical Physics Letters

      Volume: 705 Pages: 85-91

    • DOI

      10.1016/j.cplett.2018.05.046

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of approximate spin projection method and its application for elucidation of electronic structures, molecular structures and physical properties of polynuclear metal complexes2018

    • Author(s)
      Kitagawa Yasutaka、Saito Toru、Yamaguchi Kizashi
    • Journal Title

      Bull. Jpn. Soc. Coord. Chem.

      Volume: 71 Issue: 0 Pages: 57-68

    • DOI

      10.4019/bjscc.71.57

    • NAID

      130007438867

    • ISSN
      1882-6954, 1883-1737
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Concerted Mechanism of Water Insertion and O2 Release during the S4 to S0 Transition of the Oxygen-Evolving Complex in Photosystem?II2018

    • Author(s)
      Shoji Mitsuo、Isobe Hiroshi、Shigeta Yasuteru、Nakajima Takahito、Yamaguchi Kizashi
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 122 Pages: 6491-6502

    • DOI

      10.1021/acs.jpcb.8b03465

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Approximate Spin Projection for Broken-Symmetry Method and Its Application2018

    • Author(s)
      Kitagawa Yasutaka、Saito Toru、Yamaguchi Kizashi
    • Journal Title

      IntechOpen

      Volume: - Pages: 122-139

    • DOI

      10.5772/intechopen.75726

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structured near-infrared Magnetic Circular Dichroism spectra of the Mn 4 CaO 5 cluster of PSII in T. vulcanus are dominated by Mn(IV) d-d ‘spin-flip’ transitions2018

    • Author(s)
      Morton J., Chrysina M., Craig V. S.J., Akita F., Nakajima Y., Lubitz W., Cox N., Shen J.-R., and Krausz E.
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Bioenergetics

      Volume: 1859 Pages: 88-98

    • DOI

      10.1016/j.bbabio.2017.10.004

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An aluminum/cobalt/iron/nickel alloy as a precatalyst for water oxidation2018

    • Author(s)
      Najafpour M. M., Mehrabani S., Bagheri R., Song Z., Shen J.-R., Allakhverdiev S. I.
    • Journal Title

      Inter. J. Hydrogen Energy

      Volume: 43 Pages: 2083-2090

    • DOI

      10.1016/j.ijhydene.2017.12.025

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The deep red state of photosystem II in Cyanidioschyzon merolae2018

    • Author(s)
      Langley J., Morton J., Purchase R., Tian L., Shen L., Han G., Shen J.-R., Krausz E.
    • Journal Title

      Photosynthetica

      Volume: 56 Pages: 275-278

    • DOI

      10.1007/s11099-017-0760-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Rate-limiting steps in the dark-to-light transition of Photosystem II - revealed by chlorophyll-a fluorescence induction2018

    • Author(s)
      Magyar M., Sipka G., Kovacs L., Ughy B., Zhu Q., Han G., Spunda V., Lambrev P. H., Shen J.-R., Garab G.
    • Journal Title

      Scientific Reports

      Volume: 8 Pages: 2755-2755

    • DOI

      10.1038/s41598-018-21195-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Theory of chemical bonds in metalloenzymes XXI. Possible mechanisms of water oxidation in oxygen evolving complex of photosystem II2018

    • Author(s)
      K. Yamaguchi, M. Shoji, H. Isobe, S. Yamanaka, T. Kawakami, S. Yamada, M. Katouda, T. Nakajima
    • Journal Title

      Molecular Physics

      Volume: 116 Pages: 717-745

    • DOI

      10.1080/00268976.2018.1428375

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional role of Lys residues of Psb31 in electrostatic interactions with diatom photosystem II2017

    • Author(s)
      Nagao Ryo、Suzuki Takehiro、Dohmae Naoshi、Shen Jian-Ren、Tomo Tatsuya
    • Journal Title

      FEBS Lett.

      Volume: 591 Pages: 3259-3264

    • DOI

      10.1002/1873-3468.12830

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Probing structure-function relationships in early events in photosynthesis using a chimeric photocomplex2017

    • Author(s)
      Nagashima K. V. P., Sasaki M., Hashimoto K., Takaichi S., Nagashima S., Yu L. -J., Abe Y., Gotou K., Kawakami T., Takenouchi, M. Shibuya Y., Yamaguchi A., Ohno T., Shen J. -R., Inoue K., Madigan M., Kimura Y., Wang-Otomo Z. -Y.
    • Journal Title

      Proc. Natl. Acad. Sci. USA.

      Volume: 114 Pages: 10906-10911

    • DOI

      10.1073/pnas.1703584114

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The crystal structure of Deg9 reveals a novel octameric-type HtrA protease2017

    • Author(s)
      Ouyang M., Li X., Zhao S., Pu H., Shen J. -R., Adam Z., Clausen T., Zhang L.
    • Journal Title

      Nature Plants

      Volume: 3 Pages: 973-982

    • DOI

      10.1038/s41477-017-0060-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 天然光合成における高効率光捕集・水分解の反応機構2019

    • Author(s)
      沈 建仁
    • Organizer
      平成30年度 産総研中国センター国際シンポジウム「SDGsの推進に資する化学技術と材料/タンパク質構造解析が切り拓く低環境負荷社会」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Mechanism of water-splitting catalyzed by the Mn4CaO5-cluster of photosystem II2019

    • Author(s)
      Jian-Ren Shen
    • Organizer
      10th OCARINA International Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 巨大膜タンパク質複合体の高分解能構造解析から探る光合成の仕組み2019

    • Author(s)
      沈 建仁
    • Organizer
      平成 30 年度第 2回構造生物構造生物学研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Structural studies of large membrane-protein complexes involved in photosynthesis by a combination of X-ray crystallography and cryo-EM2019

    • Author(s)
      沈 建仁
    • Organizer
      理化学研究所RSCクライオ電子顕微鏡シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 光合成で働く光化学系II・酸素発生クラスターの構造とMn原子の価数2019

    • Author(s)
      神谷信夫
    • Organizer
      触媒学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Mechanism of photosynthetic water-splitting catalyzed by the Mn4CaO5 metal cluster of photosystem II2018

    • Author(s)
      J.-R Shen, F. Akita, M. Suga
    • Organizer
      1st Asia-Oceania International Congress on Photosynthesis (AOICP2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Crystal structure of LH1-RC complex from a purple sulfur bacterium Thermochromatium tedium2018

    • Author(s)
      L.-J. Yu, M. Suga, T. Kawakami, Y. Kimura, Z.-Y. Wang-Otomo, J.-R. Shen
    • Organizer
      1st Asia-Oceania International Congress on Photosynthesis (AOICP2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Crystal structure of plant PSI-LHCI supercomplex and its energy transfer mechanism2018

    • Author(s)
      X. Qin, M. Suga, T. Kuang, J.-R. Shen
    • Organizer
      1st Asia-Oceania International Congress on Photosynthesis (AOICP2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanism of photosynthetic water-splitting catalyzed by the Mn4CaO5 metal cluster in photosystem II2018

    • Author(s)
      Shen J.-R.
    • Organizer
      20th European Bioenergetics Conference (EBEC2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanism of photosynthetic water oxidation2018

    • Author(s)
      Shen J.-R.
    • Organizer
      Japan-Finland Seminar 2018 “Shaping photosynthesis against climate change and toward efficient water and nutrient management”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structural biological studies of photosynthetic membrane-protein complexes2018

    • Author(s)
      Shen J.-R.
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogensis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 中性子回折による光化学系II光誘導水分解・酸素発生反応機構解明への期待2018

    • Author(s)
      沈 建仁
    • Organizer
      iBIX将来構想検討会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 光合成における光誘導水分解・酸素発生反応の分子機構2018

    • Author(s)
      沈 建仁
    • Organizer
      静岡大学理学部講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Structural biological studies on photosynthetic membrane-protein complexes2018

    • Author(s)
      Jian-Ren Shen
    • Organizer
      The 9th International Conference on Photosynthesis and Hydrogen Energy Research for Sustainability
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Large-scale QM/MM calculations of the CaMn4O5 cluster in the S3 state of oxygen evolving complex of photosystem II2018

    • Author(s)
      Kizashi Yamaguchi, Mitsuo Shoji, Hiroshi Isobe, Michi Suga, Fusamichi Akita, Jian-Ren Shen and Takahito Nakajima
    • Organizer
      The 1st European Congress on Photosynthesis Research ePS-1
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新学術領域研究内容の説明と研究グループの研究紹介2018

    • Author(s)
      沈 建仁
    • Organizer
      科研費・新学術領域「革新的光物質変換」第1回公開シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] SQDG欠損変異株由来PSIIの結晶構造と機能解析2018

    • Author(s)
      中島芳樹, 梅名泰史, 遠藤嘉一郎, 長尾遼, 小林康一, 野口巧, 和田元, 沈建仁
    • Organizer
      科研費・新学術領域「革新的光物質変換」第1回公開シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Structure of photosynthetic LH1-RC complex at 1.9 A resolution2018

    • Author(s)
      L.-J. Yu, M. Suga1, Z.-Y. Wang-Otomo, J.-R. Shen
    • Organizer
      科研費・新学術領域「革新的光物質変換」第1回公開シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Mechanism of photosynthetic water-splitting catalyzed by the Mn4CaO5 metal cluster in photosystem II2018

    • Author(s)
      Jian-Ren Shen
    • Organizer
      Gordon Research Conference on Metals in Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanism of water oxidation in natural photosynthesis catalyzed by the Mn4CaO5-cluster of photosystem II2018

    • Author(s)
      Jian-Ren Shen
    • Organizer
      Gordon Research Conference on Renewable Energy: Solar Fuels
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanism of photosynthetic water oxidation based on the structural analyses of photosystem II2018

    • Author(s)
      Jian-Ren Shen
    • Organizer
      5th International Workshop on Solar Energy for Sustainability, Bioenergetics: Oxygen Production and Reduction
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Crystal structure of PSII in the intermediate states and possible mechanism of O=O bond formation2018

    • Author(s)
      M. Suga, F. Akita, J.-R. Shen
    • Organizer
      5th International Workshop on Solar Energy for Sustainability, Bioenergetics: Oxygen Production and Reduction
    • Related Report
      2017 Annual Research Report
  • [Presentation] シリアルフェムト秒結晶解析により明らかにした光化学系IIの反応中間体の立体構造と酸素発生機構2018

    • Author(s)
      菅倫寛,秋田総理,菅原道泰,久保稔,中島芳樹,岩田想,沈建仁
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 好熱性シアノバクテリアThermosynechococcus elongatusにおけるマイナーフェレドキシンの構造解析2018

    • Author(s)
      本村大樹,Lidia Zuccarello,Alain Boussac,沈建仁
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 集光性フコキサンチンタンパク質と光化学系Iコアとの相互作用2018

    • Author(s)
      長尾遼,植野嘉文,秋本誠志,秋田総理,沈建仁
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光合成水分解反応の仕組み-植物に学ぶ 光エネルギーの高効率利用(みどりの学術賞受賞講演)2017

    • Author(s)
      沈 建仁
    • Organizer
      平成29年度みどりの学術賞受賞記念講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Mechanism of photosynthetic water-splitting from a structural point of view2017

    • Author(s)
      Jian-Ren Shen
    • Organizer
      Gordon Research Conference on Photosynthesis
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanism of photosynthetic water oxidation based on atomic structure of photosystem II2017

    • Author(s)
      Jian-Ren Shen
    • Organizer
      IBC2017, The XIX International Botanical Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物に学ぶ太陽光エネルギーの高効率利用ー岡山県三木記念賞受賞について2017

    • Author(s)
      沈 建仁
    • Organizer
      岡山ロータリークラブ例会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 新学術領域研究内容の紹介2017

    • Author(s)
      沈 建仁
    • Organizer
      科研費・新学術領域「革新的光物質変換」キックオフミーティング
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光合成光化学系IIと光化学系I複合体の構造と機能に関する研究(植物学会学術賞受賞講演)2017

    • Author(s)
      沈 建仁
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 光合成における光水分解反応の機構解明(錯体化学会貢献賞受賞講演)2017

    • Author(s)
      沈 建仁
    • Organizer
      錯体化学討論会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] XFELによって明らかになった光化学系II水分解触媒の中間体構造と反応機構2017

    • Author(s)
      沈 建仁
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Molecular mechanism of photosynthetic water-splitting based on the atomic structure of photosystem II2017

    • Author(s)
      Jian-Ren Shen
    • Organizer
      15th Chinese and International Biophysics Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光合成水分解の仕組み ー光と水からエネルギーと酸素へ2017

    • Author(s)
      沈 建仁
    • Organizer
      東京都中央区区民カレッジに「科学技術の最前線」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 光合成の仕組みに迫る2017

    • Author(s)
      沈 建仁
    • Organizer
      JSTサイエンスアゴラ2017 越境する光科学
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Mechanism of light-induced water-splitting in natural photosynthesis2017

    • Author(s)
      Jian-Ren Shen
    • Organizer
      2nd International RINS Symposium (Okayama Science University)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光合成水分解反応の機構解明と革新的光物質変換2017

    • Author(s)
      沈 建仁
    • Organizer
      植物科学シンポジウム2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Structures of PSII with herbicides bound2017

    • Author(s)
      Jian-Ren Shen
    • Organizer
      Japan-France Joint Workshop on the Structure and Function of Photosystem II
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanism of photosynthetic water oxidation based on the structural analysis of photosystem II2017

    • Author(s)
      Jian-Ren Shen
    • Organizer
      The Second International Symposium on Biofunctional Chemistry (ISBC2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Methods in Enzymology - 613, Enzymes of Energy Technology2018

    • Author(s)
      Keisuke Kawakamia, Jian-Ren Shen
    • Publisher
      Academic Press
    • Related Report
      2018 Annual Research Report
  • [Remarks] 新学術領域研究ホームページ

    • URL

      http://photoenergy-conv.net/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 新学術領域ホームページ

    • URL

      http://photoenergy-conv.net/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-07-04   Modified: 2020-08-26  

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