| Project Area | Innovative nanoscience of supermolecular motor proteins working in biomembranes |
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| Project/Area Number |
18074002
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
HISABORI Toru 東京工業大学, 資源科学研究所, 教授 (40181094)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Yasuyuki 立教大学, 理学部, 准教授 (80386507)
KONNO Hiroki 東京工業大学, 資源化学研究所, 助教 (80419267)
YAGI Hiromasa 大阪大学, 蛋白質研究所, 助教 (70332749)
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| Co-Investigator(Renkei-kenkyūsha) |
KONNO Hiroki 東京工業大学, 資源化学研究所, 助教 (80419267)
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| Project Period (FY) |
2006 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥110,000,000 (Direct Cost: ¥110,000,000)
Fiscal Year 2010: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2009: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2008: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2007: ¥23,700,000 (Direct Cost: ¥23,700,000)
Fiscal Year 2006: ¥23,300,000 (Direct Cost: ¥23,300,000)
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| Keywords | ATP合成酵素 / 調節機構 / εサブユニット / 構造変化 / レドックス制御 / γサブユニット / 酸化還元調節 / 1分子観察 / 細胞内ATP量 / 回転制御 / 回転阻害 / テントキシン / レドックス調節 |
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| Research Abstract |
In order to understand the regulation mechanism of the molecular motor enzyme, F_1-ATPase, we studied the regulation of rotation of the γ subunit itself and external regulation mechanism as well. Consequently, we obtained the following results: 1. Based on the single molecule analysis of rotation of the γ subunit caused by the conformational change of this subunit, we revealed the relationship between the pause position and the form of the β subunit. 2. The inhibitory function of the intrinsic inhibitory subunit ε was studied both biochemical method and biophysical method. 3. Based on the study of the intracellular ATP level and the ATP synthase activity, physiological significance of the regulation of ATP synthase was discussed.
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