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Mechanics of supermolecular motor proteins revealed by an advanced optical microscope designed to track three dimensional dynamics.

Planned Research

Project AreaInnovative nanoscience of supermolecular motor proteins working in biomembranes
Project/Area Number 18074008
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionGakushuin University

Principal Investigator

NISHIZAKA Takayuki  Gakushuin University, 理学部, 教授 (40359112)

Co-Investigator(Kenkyū-buntansha) MASAIKE Tomoko  学習院大学, 理学部, 助教 (60406882)
矢島 潤一郎  学習院大学, 理学部, 研究員 (00453499)
Co-Investigator(Renkei-kenkyūsha) YAJIMA Junichiro  学習院大学
ADACHI Kengo  学習院大学
SUGAWA Mitsuhiro  学習院大学
Research Collaborator HORIBE Keiko  学習院大学
TATSUGUCHI Ayako  学習院大学
Project Period (FY) 2006 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥112,400,000 (Direct Cost: ¥112,400,000)
Fiscal Year 2010: ¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2009: ¥23,300,000 (Direct Cost: ¥23,300,000)
Fiscal Year 2008: ¥24,300,000 (Direct Cost: ¥24,300,000)
Fiscal Year 2007: ¥24,300,000 (Direct Cost: ¥24,300,000)
Fiscal Year 2006: ¥24,300,000 (Direct Cost: ¥24,300,000)
Keywords1分子生物物理学 / 光学顕微鏡 / 分子モーター / タンパク質科学 / 顕微操作 / 全反射型顕微鏡 / 光ピンセット / 3次元検出 / F1-ATPase / タンパク質構造 / 1分子ナノ計測 / バイオイメージング / 1分子計測・操作 / 構造生物学 / タンパク質 / F_1ATPase / 蛋白質の構造
Research Abstract

The final goal of our group is to establish the missing link between chemical reaction and mechanical event in molecular motors. To achieve this, we have developed advanced versions of conventional optical microscopes and applied them into single-molecule techniques. Our main contributions are following two studies: one is about the kinesin-microtubule system and the other F1-ATPase. These techniques are applicable to other molecular machines, hopefully in more sophisticated ways, and we hope to investigate this in future studies as a new research field 'single-molecule structural biology.'

Report

(7 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (126 results)

All 2011 2010 2009 2008 2007 2006 2005 Other

All Journal Article (31 results) (of which Peer Reviewed: 12 results) Presentation (76 results) Remarks (9 results) Patent(Industrial Property Rights) (10 results) (of which Overseas: 4 results)

  • [Journal Article] Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells.2011

    • Author(s)
      Tsuji, T., S.Kawai-Noma, C.G.Pack, H.Terajima, J.Yajima, T.Nishizaka, M.Kinjo, H.Taguchi
    • Journal Title

      Biochem Biophys Res Commun 405(4)

      Pages: 638-643

    • Related Report
      2010 Final Research Report
  • [Journal Article] Molecular Bio-Motors in Living Cells.2010

    • Author(s)
      Nishizaka, T.
    • Journal Title

      Adv.Biochem.Eng.Biotechnol.

    • Related Report
      2010 Final Research Report
  • [Journal Article] Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells2010

    • Author(s)
      Tsuji, T., S.Kawai-Noma, C.G.Pack, H.Terajima, J.Yajima, T.Nishizaka, M.Kinjo, H.Taguchi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 405 Pages: 638-43

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] タンパク質1分子とその中身を観察する顕微鏡技術2010

    • Author(s)
      西坂崇之、政池知子
    • Journal Title

      顕微鏡

      Volume: 45 Pages: 173-9

    • NAID

      130007788887

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 科学の"雲の通ひ路"2010

    • Author(s)
      西坂崇之
    • Journal Title

      生物物理

      Volume: 50 Pages: 214-5

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane.2009

    • Author(s)
      Tabata, K.V., K.Sato, T.Ide, T.Nishizaka, A.Nakano, H.Noji
    • Journal Title

      EMBO J 28(21)

      Pages: 3279-3289

    • Related Report
      2010 Final Research Report
  • [Journal Article] 蛋白質の局所構造変化を1個のレベルで可視化する2009

    • Author(s)
      政池知子、西坂崇之
    • Journal Title

      蛋白質・核酸・酵素 54

      Pages: 1919-1927

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane2009

    • Author(s)
      田端和仁、佐藤健、井出徹、西坂崇之、中野明彦、野地博行
    • Journal Title

      EMBO Journal 28

      Pages: 3279-3289

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking.2008

    • Author(s)
      Yajima, J., K.Mizutani, T.Nishizaka
    • Journal Title

      Nat.Struct.Mol.Biol. 15(10)

      Pages: 1119-1121

    • Related Report
      2010 Final Research Report
  • [Journal Article] Cooperative three-step motions in catalytic subunits of F1-ATPase correlate with 80° and 40° substep rotations.2008

    • Author(s)
      Masaike, T., F.Koyama-Horibe, K.Oiwa, M.Yoshida, T.Nishizaka
    • Journal Title

      Nat.Struct.Mol.Biol. 15(12)

      Pages: 1326-1333

    • Related Report
      2010 Final Research Report
  • [Journal Article] Coperative three-step motions in catalytic subunits of F1-ATPase correlate with 80° and 40° substep rotations2008

    • Author(s)
      Masaike, T., Koyama-Horibe, F., Oiwa, K., Yoshida M. & Nishizaka, T.
    • Journal Title

      Nat. Struct. Mol. Biol. 15

      Pages: 1326-1333

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] A torque component present in kinesin-5 revealed by 3D tracking2008

    • Author(s)
      Yajima, J., Mizutani, K. & Nishizaka, T.
    • Journal Title

      Nat. Struct. Mol. Biol. 15

      Pages: 1119-1121

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking2008

    • Author(s)
      矢島潤一郎、水谷佳奈、西坂崇之
    • Journal Title

      Nature Structural and Molecular Biology 15

      Pages: 1119-1121

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cooperative three-step motions in catalytic subunits of Fl-AT Pase correlate with 80° and 40° substep rotations2008

    • Author(s)
      政池知子、小山一堀部史恵、大岩和弘、吉田賢右、西坂崇之
    • Journal Title

      Nature Structural and Molecular Biology 15

      Pages: 1326-1333

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microtubule sliding and rotation driven by single headed kinesins2008

    • Author(s)
      矢島潤一郎, 他2名
    • Journal Title

      Biophysical Journal Jan(abstr.)

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Single-Molecule Observation of Rotation of F1-ATPase Through Microbeads.2007

    • Author(s)
      Nishizaka, T., K.Mizutani, T.Masaike
    • Journal Title

      Methods Mol.Biol. 392

      Pages: 171-182

    • Related Report
      2010 Final Research Report
  • [Journal Article] 化学反応の1分子イメージング技術2007

    • Author(s)
      Nishizaka T., T.Masaike
    • Journal Title

      蛋白質・核酸・酵素 52(4)

      Pages: 317-322

    • Related Report
      2010 Final Research Report
  • [Journal Article] F1-ATPaseの化学-力学カップリング:1分子の反応を顕微鏡でとらえる2007

    • Author(s)
      西坂崇之、政池知子
    • Journal Title

      生物物理 47

      Pages: 118-123

    • Related Report
      2010 Final Research Report
  • [Journal Article] Single Molecule Energetics of F1-ATPase Motor.2007

    • Author(s)
      Muneyuki, E., T.Watanabe-Nakayama, T.Suzuki, M.Yoshida, T.Nishizaka, H.Noji
    • Journal Title

      Biophys J 92(5)

      Pages: 1806-1812

    • Related Report
      2010 Final Research Report
  • [Journal Article] Jr., Coupling of rotation and catalysis in F1-ATPase revealed by single-molecule imaging and manipulation.2007

    • Author(s)
      Adachi, K., K.Oiwa, T.Nishizaka, S.Furuike, H.Noji, H.Itoh, M.Yoshida, K.Kinosita
    • Journal Title

      Cell 130(2)

      Pages: 309-321

    • Related Report
      2010 Final Research Report
  • [Journal Article] Coupling of Rotation and Catalysis in F1-ATPase Revealed by Single-Molecule Imaging and Manipulation2007

    • Author(s)
      Adachi, K., Oiwa, K., Nishizaka, T., Furuike, S., Noji, H., Itoh, H., Yoshida, M., and Kinosita, K., Jr.
    • Journal Title

      Cell 130

      Pages: 309-321

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] F1-ATPaseの化学-力学カップリング : 1分子の反応を顕微鏡でとらえる2007

    • Author(s)
      西坂崇之、政池知子
    • Journal Title

      生物物理 47

      Pages: 118-123

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] 化学反応の1分子イメージング技術 -回転分子モーターへの応用-2007

    • Author(s)
      西坂崇之、政池知子
    • Journal Title

      蛋白質・核酸・酵素 52

      Pages: 317-322

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] 化学反応の1分子イメージング技術2007

    • Author(s)
      西坂崇之、政池知子
    • Journal Title

      蛋白質 核酸 酵素 52

      Pages: 317-322

    • Related Report
      2007 Annual Research Report
  • [Journal Article] F1-ATPaseの化学-力学カップリング2007

    • Author(s)
      西坂崇之、政池知子
    • Journal Title

      生物物理 47

      Pages: 118-123

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Coupling of Rotation and Catalysis in F1-ATPase Revealed by Single-Molecule Imaging and Manipulation2007

    • Author(s)
      足立健吾, 他7名
    • Journal Title

      Cell 130

      Pages: 309-321

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single Molecule Energetics of F_1-ATPase Motor.2007

    • Author(s)
      宗行英郎(他5名)
    • Journal Title

      Biophysical Journal 92

      Pages: 1806-1812

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Tracking of 3-D motion with nanometer accuracy.2007

    • Author(s)
      水谷佳奈(他3名)
    • Journal Title

      Biophysical Journal Jan(abstr.)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 化学反応の1分子イメージング技術2007

    • Author(s)
      西坂宗之(他1名)
    • Journal Title

      蛋白質 核酸 酵素 52

      Pages: 317-322

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Single-molecule observation of rotation of F_1 ATPase through micro beads.2007

    • Author(s)
      西坂宗之(他2名)
    • Journal Title

      Methods in Molecular Biology (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 化学反応の1分子イメージング技術2007

    • Author(s)
      西坂宗之(他1名)
    • Journal Title

      生物物理 (in press)(総説)

    • Related Report
      2006 Annual Research Report
  • [Presentation] Orientation of the g shaft and catalytic b subunit in F1-ATPase in the intermediate state revealed at the single-molecule level2011

    • Author(s)
      西坂崇之, 須河光弘, 政池知子
    • Organizer
      Biophysical Society 55th Annual Meeting
    • Place of Presentation
      Baltimore Convention Center, Maryland, USA
    • Year and Date
      2011-03-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Imaging structure and function of motor proteins under optical microscopes.2011

    • Author(s)
      西坂崇之, 矢島潤一郎, 須河光弘, 足立健吾, 政池知子
    • Organizer
      7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS), India Habitat Center
    • Place of Presentation
      New Delhi, India
    • Year and Date
      2011-02-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Imaging structure and function of motor proteins under optical microscopes2011

    • Author(s)
      西坂崇之, 矢島潤一郎, 須河光弘, 足立健吾, 政池知子
    • Organizer
      7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS)
    • Place of Presentation
      India Habitat Center, New Delhi, India
    • Year and Date
      2011-02-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分子モーターの動態を可視化する光学顕微鏡技術2011

    • Author(s)
      西坂崇之
    • Organizer
      第5回NIBBバイオイメージングフォーラム
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2011-01-11
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] キネシンモーターによる微小管の並進回転運動メカニズム2011

    • Author(s)
      矢島潤一郎, 西坂崇之
    • Organizer
      生体運動研究合同班・2011年班会議
    • Place of Presentation
      大阪市立大学・杉本キャンパス
    • Year and Date
      2011-01-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分子モーターの動態を可視化する光学顕微鏡技術2011

    • Author(s)
      西坂崇之, 政池知子
    • Organizer
      生体運動研究合同班・2011年班会議
    • Place of Presentation
      大阪市立大学・杉本キャンパス
    • Year and Date
      2011-01-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 回転分子モーターF1-ATPaseのイメージングImaging of rotary molecular motor F1-ATPase.2010

    • Author(s)
      政池知子, 龍口文子, 西坂崇之
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学・川内北キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Imaging of rotary molecular motor F1-ATPase2010

    • Author(s)
      政池知子, 龍口文子, 西坂崇之
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学・川内北キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] High-speed imaging of single fluorescently labeled nucleotides bound to F1-ATPase2010

    • Author(s)
      橋本優, 足立健吾, 政池知子, 西坂崇之
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学・川内北キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Tilting of the g subunit and conformational changes of the a3b3 in F1-ATPase2010

    • Author(s)
      須河光弘, 岡田薫, 小林大, 政池知子, 西坂崇之
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学・川内北キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Application of 3-D prismatic optical tracking : single-molecule optical tweezers approach and stereoscopic 3-D view2010

    • Author(s)
      西坂崇之, 足立健吾, 龍口文子, 政池知子
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学・川内北キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Corkscrew motion of a sliding microtubule driven by single-headed kinesins with mutations in N-terminal region2010

    • Author(s)
      大和田慎介, 矢島潤一郎, 政池知子, 西坂崇之
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学・川内北キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Localization and orientation of single fluorescent nucleotides bound to the catalytic sites in rotary motor F1-ATPase2010

    • Author(s)
      足立健吾, 政池知子, 大岩和宏, 西坂崇之
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学・川内北キャンパス
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] プリズムによる3次元光学トラッキング技術の応用/1分子を対象にした光ピンセット操作と3次元立体視表示Application of 3-D prismatic optical tracking : single-molecule optical tweezers approach and stereoscopic 3-D view.2010

    • Author(s)
      西坂崇之, 足立健吾, 龍口文子, 政池知子
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      東北大学・川内北キャンパス
    • Year and Date
      2010-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 3次元光ピンセットの分子モーターヘの応用2010

    • Author(s)
      西坂崇之、龍口文子
    • Organizer
      生体運動研究合同班・2010年班会議
    • Place of Presentation
      中央大学理工学部
    • Year and Date
      2010-01-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] マイコプラズマのステップ2010

    • Author(s)
      中根大介、西坂崇之、宮田真人
    • Organizer
      生体運動研究合同班・2010年班会議
    • Place of Presentation
      中央大学理工学部
    • Year and Date
      2010-01-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Imaging function of motor proteins under optical microscopes2009

    • Author(s)
      西坂崇之
    • Organizer
      Intemational workshop on dynamic cross-effect in softly condensed matter
    • Place of Presentation
      国立情報学研究所
    • Year and Date
      2009-11-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Imaging function of motor proteins under optical microscopes2009

    • Author(s)
      西坂崇之
    • Organizer
      International workshop on dynamic cross-effect in softly condensed matter
    • Place of Presentation
      国立情報学研究所
    • Year and Date
      2009-11-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] "Visualization of COPII vesicle formation process reconstituted in the artificial lipid bilayer"「人工脂質二重膜上に再構成したCOPII小胞形成過程のイメージング」2009

    • Author(s)
      田端和仁、佐藤健、井出徹、西坂崇之、中野明彦、野地博行
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2009-10-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Visualization of COPII vesicle formation process reconstituted in the artificial lipid bilayer2009

    • Author(s)
      田端和仁、佐藤健、井出徹、西坂崇之、中野明彦、野地博行
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Step detection of gliding bacterium, Mycoplasma mobile2009

    • Author(s)
      中根大介、西坂崇之、宮田真人
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] High-accuracy localization of single nucleotides bound to the catalytic sites in rotary motor F1-ATPase2009

    • Author(s)
      足立健吾、政池知子、大岩和宏、西坂崇之
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Versatility of the unbinding force measurements at the single molecule level adapted to different molecular motors2009

    • Author(s)
      Sergey Mikhailenko, Oguchi, Y., Ohki, T., Olivares, A.O., De La Cruz, E.M., Ishiwata, S., Nishizaka, T.
    • Organizer
      日本生物物群会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Conformational changes of the β subunits in F1-ATPase revealed by single molecule FRET measurement2009

    • Author(s)
      須河光弘、小林大、西坂崇之、政池知子
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Characterization of a torque component present in the kinesins-driven microtubule movement2009

    • Author(s)
      矢島潤一郎、西坂崇之
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Imaging structure and function of motor proteins at the single molecular level2009

    • Author(s)
      西坂崇之、足立健吾、矢島潤一郎、Sergey V.Mikhailenko、須河光則、政池知子
    • Organizer
      Intemational Symposium : Innovative Nanoscience of Supermolecular Motor Proteins
    • Place of Presentation
      京都芝蘭会館
    • Year and Date
      2009-09-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Imaging structure and function of motor proteins at the single molecular level2009

    • Author(s)
      西坂崇之、足立健吾、矢島潤一郎、Sergey V. Mikhailenko、須河光則、政池知子
    • Organizer
      International Symposium : Innovative Nanoscience of Supermolecular Motor Proteins
    • Place of Presentation
      京都芝蘭会館
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光学顕微鏡による分子モーターの構造機能相関の可視化2009

    • Author(s)
      西坂崇之
    • Organizer
      生理学研究所研究会「作動中の膜機能分子の姿を捉える-静止画から動画へ-」自然科学研究機構
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2009-09-03
    • Related Report
      2010 Final Research Report
  • [Presentation] 光学顕微鏡による分子モーターの構造機能相関の可視化2009

    • Author(s)
      西坂崇之
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      自然科学研究機構岡崎コンファレンスセンター
    • Year and Date
      2009-09-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Imaging structure and fUnction of motor proteins at the single molecular level2009

    • Author(s)
      西坂崇之、矢島潤一郎、足立健吾、政池知子
    • Organizer
      TSRC Workshop on Protein Dynamics, Telluride Science Research Center
    • Place of Presentation
      Telluride U.S.A.
    • Year and Date
      2009-08-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Imaging structure and function of motor proteins at the single molecular level2009

    • Author(s)
      西坂崇之、矢島潤一郎、足立健吾、政池知子
    • Organizer
      TSRC Workshop on Protein Dynamics
    • Place of Presentation
      Telluride Science Research Center
    • Year and Date
      2009-08-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] Visualizing structure dynamics and function in single motor proteins2009

    • Author(s)
      西坂崇之、矢島潤一郎、足立健吾、政池知子
    • Organizer
      The 4th Asian And Pacific Rim Symposium On Biophotonics
    • Place of Presentation
      The Shilla Jeju, South Korea
    • Year and Date
      2009-05-28
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 触媒サブユニットの構造変化から探るF1-ATPaseの回転駆動メカニズム2009

    • Author(s)
      政池知子、小山史恵、龍口文子、大岩和弘、吉田賢右、西坂崇之
    • Organizer
      第9回日本蛋白質学会年会、シンポジウム「タンパク質のダイナミクス計測のためのイメージング技術」
    • Place of Presentation
      熊本全日空ホテルニュースカイ
    • Year and Date
      2009-05-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 触媒サブユニットの構造変化から探るFi-ATPaseの回転駆動メカニズム2009

    • Author(s)
      政池知子、小山史恵、龍口文子、大岩和弘、吉田賢右、西坂崇之
    • Organizer
      日本蛋白質学会年会
    • Place of Presentation
      熊本全日空ホテルニュースカイ
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] Visualizing structure dynamics and function in single motor proteins2009

    • Author(s)
      西坂崇之、矢島潤一郎、足立健吾、政池知子
    • Organizer
      The 4th Asian And Pacific Rim Symposium On Biophotonics, The Shilla Jeju
    • Place of Presentation
      South Korea
    • Year and Date
      2009-03-28
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Imaging structure and function of motor proteins by using advanced optical microscopes2009

    • Author(s)
      西坂崇之、矢島潤一郎、Sergey V.Mikhailenko、政池知子
    • Organizer
      The 6th Asian Biophysics Association(ABA)Symposium
    • Place of Presentation
      HKUST, Hong Kong
    • Year and Date
      2009-01-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Imaging structure and function of motor proteins by using advanced optical microscopes2009

    • Author(s)
      西坂崇之、矢島潤一郎、Sergey V. Mikhailenko、政池知子
    • Organizer
      The 6th Asian Biophysics Association Symposium
    • Place of Presentation
      HKUST, Hong Kong
    • Year and Date
      2009-01-13
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] Imaging structure and function of motor proteins by using advanced optical microscopes2009

    • Author(s)
      西坂崇之、他3名
    • Organizer
      The 6th Asian Biophysics Association (ABA) Symposium
    • Place of Presentation
      HKUST, Hong Kong
    • Year and Date
      2009-01-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] 生体分子の構造と機能を1個のレベルで可視化する2008

    • Author(s)
      西坂崇之、矢島潤一郎、政池知子
    • Organizer
      BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)シンポジウム「生命システムの階層間をまたぐイメージング技術」
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] 生体分子の構造と機能を1個のレベルで可視化する2008

    • Author(s)
      西坂崇之、矢島潤一郎、政池知子
    • Organizer
      BMB2008(第31回 日本分子生物学会年会・第81回日本生化学会大会 合同大会)シンポジウム「生命システムの階層間をまたぐイメージング技術」
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] 生体分子の構造と機能を1個のレベルで可視化する2008

    • Author(s)
      西坂崇之、矢島潤一郎、政池知子
    • Organizer
      BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Displacement of beads attached on red blood cell caused by Mycoplasma mobile gliding2008

    • Author(s)
      中根大介、西坂崇之、宮田真人
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Exclusion of non-transport protein on COPII vesicle for mation proces2008

    • Author(s)
      田端和仁、他5名
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Detection of conformational changes of the o subunits of F1-ATPase by FRET2008

    • Author(s)
      小林大、西坂崇之、政池知子
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Angle Dependence of the Affinity for Nucleotides in Rotary Motor F1-ATPase2008

    • Author(s)
      足立健吾、他6名
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Optical tweezers detecting 3D forces and its application to molecular motors2008

    • Author(s)
      寺島勲、他5名
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] The role of the axle in F1-ATPase revealed by rotation of a mutant containing a twisted a subunit2008

    • Author(s)
      平川隆之、他5名
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Three-dimensional nanometer resolution optical tracking reveals a torque component present in kinesin-5 driven movement of microtubules2008

    • Author(s)
      矢島潤一郎、水谷佳奈、西坂崇之
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Analysis of Thermal Fluctuation of Beads Bound to the Shaft of F1-ATPase2008

    • Author(s)
      西坂崇之、平川隆之、小林大、政池知子
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] F1-ATPase as a model for understanding mechanisms of enzyme functions through detection of confbrmational changes in single molecules2008

    • Author(s)
      政池知子、小山-堀部史恵、大岩和弘、吉田賢右、西坂崇之
    • Organizer
      F1-ATPaseをモデルとした1分子構造変化検出による酵素機能発現の理解」日本生物物理学会第46回年会若手奨励賞招待講演
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-03
    • Related Report
      2010 Final Research Report
  • [Presentation] F1-ATPase as a model for understanding mechanisms of enzyme functions through detection of conformational changes in single molecules2008

    • Author(s)
      政池知子、小山-堀部史恵、大岩和弘、吉田賢右、西坂崇之
    • Organizer
      日本生物物理学会第46回年会, 若手奨励賞 招待講演
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-03
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] F1-ATPase as a model for understanding mechanisms of enzyme functions through detection of conformational chan ges in single molecules2008

    • Author(s)
      政池知子、小山一堀部史恵、大岩和弘、吉田賢右、西坂崇之
    • Organizer
      日本生物物理学会第46回年会若手奨励賞招待講演
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] The role of oGlu190 in phosphate release from F1-ATPase revealed by Phosphate binding protein2008

    • Author(s)
      原田慎吾、西坂崇之、政池知子
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Direct observation of structure and function of motor proteins at single molecular level2008

    • Author(s)
      西坂崇之、矢島潤一郎、政池知子
    • Organizer
      International Symposium on Surface Science and Nanotechnologyシンポジウム講演
    • Place of Presentation
      早稲田大学
    • Year and Date
      2008-11-11
    • Related Report
      2010 Final Research Report
  • [Presentation] 光学顕微鏡を用いた1分子構造生物学への挑戦2008

    • Author(s)
      西坂崇之
    • Organizer
      第3回生体分子イメージングの新たな技術開発に関する研究会
    • Place of Presentation
      名古屋市桜華会館
    • Related Report
      2010 Final Research Report
  • [Presentation] 単頭キネシンによる微小管の並進・回転運動機構の研究2008

    • Author(s)
      矢島 潤一郎、他3名
    • Organizer
      生体運動研究合同班・2008年班会議
    • Place of Presentation
      仙台市戦災復興記念館記念ホール
    • Related Report
      2007 Annual Research Report
  • [Presentation] F_1-ATPaseの中間状態におけるγ-subunitの位置2008

    • Author(s)
      岡田 薫、他3名
    • Organizer
      生体運動研究合同班・2008年班会議
    • Place of Presentation
      仙台市戦災復興記念館記念ホール
    • Related Report
      2007 Annual Research Report
  • [Presentation] Microtubule sliding and rotation driven by single headedkinesins2008

    • Author(s)
      矢島 潤一郎、他2名
    • Organizer
      The Joint Biophysical Society 52nd Annual Meeting and 16th International Biophysics Congress
    • Place of Presentation
      Long Beach、California USA
    • Related Report
      2007 Annual Research Report
  • [Presentation] Imaging structure and function of motor proteins at the single molecular level by using advanced optical microscopes2007

    • Author(s)
      西坂崇之、宮田真人、政池知子
    • Organizer
      日本生物物理学会第45回年会シンポジウム講演
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Conformational changes of the catalytic subunit β in single F1-ATPase molecules revealed by TIRF microscopy with polarization modulation2007

    • Author(s)
      政池知子、小山史恵、吉田賢右、大岩和弘、西坂崇之
    • Organizer
      日本生物物理学会第45回年会シンポジウム講演
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Measurements of phosphate release from a mutant F1-ATPase using phosphate binding protein2007

    • Author(s)
      原田慎吾, 西坂崇之, 政池知子
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会 口頭発表
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Biomechanics of Motor Proteins on a Molecular Scale2007

    • Author(s)
      西坂崇之
    • Organizer
      The International Conference on Science of Friction
    • Place of Presentation
      伊良湖ビューホテル
    • Related Report
      2010 Final Research Report 2008 Self-evaluation Report 2007 Annual Research Report
  • [Presentation] 細胞と分子モーターの動きを画像化する新しい顕微鏡技術2007

    • Author(s)
      西坂崇之、水谷佳奈、中根大介、見理剛、矢島潤一郎、宮田真人、政池知子
    • Organizer
      日本マイコプラズマ学会・第34回学術集会
    • Place of Presentation
      紀州加太休暇村
    • Related Report
      2010 Final Research Report 2007 Annual Research Report
  • [Presentation] 光ピンセットを用いたMycoplasma mobileのゴーストの力測定2007

    • Author(s)
      鈴木純也、寺島勲、水谷佳奈、政池知子、宮田真人、西坂崇之
    • Organizer
      日本マイコプラズマ学会・第34回学術集会
    • Place of Presentation
      紀州加太休暇村
    • Related Report
      2010 Final Research Report 2007 Annual Research Report
  • [Presentation] Measurements of phosphate release from a mutant F_1-ATPase using phosphate binding protein2007

    • Author(s)
      原田 慎吾、他2名
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Conformational changes of the catalytic subunit β in single F_1-ATPase molecules revealed by TIRF microscopy with polarization modulation2007

    • Author(s)
      政池 知子、他4名
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Microtubule sliding and rotation driven by multiplesingle-headed rat kinesins2007

    • Author(s)
      矢島 潤一郎、他2名
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Three-dimensional imaging of microtubule sliding generated by molecular motors2007

    • Author(s)
      水谷 佳奈、他5名
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Orientation of γ Subunit in F_1-ATPase in the Intermediate State2007

    • Author(s)
      岡田 薫、他3名
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Stepwise changes in distance between two βs of rotating F_1-ATPase revealed by single-pair FRET2007

    • Author(s)
      小林 大、他2名
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Measurement of three-dimensional trap stiffness in opticaltweezers2007

    • Author(s)
      寺島 勲、他4名
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Force exerted to substrate from Mycoplasma mobile,detected by red blood cell marked by plastic2007

    • Author(s)
      金宇治 綾香、他2名
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Imaging structure and function of motor proteins at the single molecular level by using advanced optical microscopes2007

    • Author(s)
      西坂 崇之、他2名
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Single Molecule Nano-Biology-Watch and Touch Single Molecules2006

    • Author(s)
      西坂崇之
    • Organizer
      JAFoS 2006(Invited Chair)
    • Place of Presentation
      Portland USA
    • Related Report
      2010 Final Research Report
  • [Presentation] 新しい顕微鏡技術を用いたFoF1-ATP合成酵素の分子内コンフォメーション変化の検出2006

    • Author(s)
      政池知子、岡田薫、水谷佳奈、吉田賢右、西坂崇之
    • Organizer
      日本分子生物学会2006フォーラム
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2010 Final Research Report
  • [Presentation] Muscle proteins as high speed nano transporters on micro pattems.2006

    • Author(s)
      吉田由美、下澤東吾、西坂崇之、石渡信一、竹内昌治
    • Organizer
      MEMS 2006
    • Place of Presentation
      Istanbul Convention & Exhibition Centre、Istanbul、Turkey
    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.gakushuin.ac.jp/univ/sci/phys/nishizaka/

    • Related Report
      2010 Final Research Report
  • [Remarks] 朝日新聞朝刊、科学欄、2009年3月9日政池知子氏の成果が報道される

    • Related Report
      2008 Self-evaluation Report
  • [Remarks] 日本生物物理学会第46回年会において、学会の若手奨励賞を政池知子氏が受賞、2008年12月4日

    • Related Report
      2008 Self-evaluation Report
  • [Remarks] 第4回日本学術振興会賞を西坂崇之が受賞、2008年3月3日、理工系「1分子レベルにおけるタンパク質の構造と機能の相関メカニズムの解明」

    • Related Report
      2008 Self-evaluation Report
  • [Remarks] 日本生物物理学会第45回年会において、学会の若手奨励賞を研究員の矢島潤一郎氏が受賞、2007年12月21日

    • Related Report
      2008 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.gakushuin.ac.jp/univ/sci/phys/nishizaka/lab/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.gakushuin.ac.jp/univ/sci/phys/nishizaka/

    • Related Report
      2007 Annual Research Report
  • [Remarks] 4/1より 学習院大学理学部教授

    • Related Report
      2007 Annual Research Report
  • [Remarks] 3/3 第4回 日本学術振興会賞 理工系 受賞

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 高時間分解能画像化方法及び装置並びに全反射型蛍光顕微鏡2010

    • Inventor(s)
      西坂崇之
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Filing Date
      2010-01-29
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 暗視野顕微鏡およびその調整方法2007

    • Inventor(s)
      西坂崇之、安田涼平
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Filing Date
      2007-05-23
    • Related Report
      2010 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 暗視野顕微鏡およびその調整方法2007

    • Inventor(s)
      西坂崇之(75%)・安田涼平(25%)
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Filing Date
      2007-05-23
    • Related Report
      2008 Self-evaluation Report
    • Overseas
  • [Patent(Industrial Property Rights)] 3次元位置観測方法及び装置2006

    • Inventor(s)
      西坂崇之
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Filing Date
      2006-06-29
    • Related Report
      2010 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 暗視野顕微鏡およびその調整方法2006

    • Inventor(s)
      西坂崇之、安田涼平
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Industrial Property Number
      2006-144050
    • Filing Date
      2006-05-24
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 3次元位置観測方法及び装置2006

    • Inventor(s)
      西坂崇之(80%)・水谷佳奈(20%)
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Filing Date
      2006-06-29
    • Related Report
      2008 Self-evaluation Report
    • Overseas
  • [Patent(Industrial Property Rights)] 3次元位置観測方法及び装置2006

    • Inventor(s)
      西坂宗之(80%), 水谷佳奈(20%)
    • Industrial Property Rights Holder
      科学技術振興機構(80%), 学習院(20%)
    • Filing Date
      2006-06-29
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 暗視野顕微鏡およびその調整方法2006

    • Inventor(s)
      西坂宗之(75%), 安田凉平(25%)
    • Industrial Property Rights Holder
      科学技術振興機構(62.5%), 学習院(37.5%)
    • Industrial Property Number
      2006-144050
    • Filing Date
      2006-05-24
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 高時間分解能画像化方法及び装置並びに全反射型蛍光顕微鏡2005

    • Inventor(s)
      西坂崇之
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構
    • Acquisition Date
      2005-03-31
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 暗視野顕微鏡およびその調整方法

    • Inventor(s)
      西坂崇之(75%)・安田涼平(25%)
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構(62.5%), 学校法人学習院(37.5%)
    • Industrial Property Number
      2006-144050
    • Related Report
      2008 Self-evaluation Report

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Published: 2006-04-01   Modified: 2018-03-28  

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