Dynamics of mitochondria in the reproduction strategy of higher plants

• Project Area: Genome Barriers in Plant Reproduction
• Project/Area Number: 18075005
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: TSUTSUMI Nobuhiro 東京大学, 大学院・農学生命科学研究科, 教授 (00202185)
• Co-Investigator(Kenkyū-buntansha): 中園 幹生 東京大学, 大学院・農学生命科学研究科, 准教授 (70282697) ; 有村 慎一 東京大学, 大学院・農学生命科学研究科, 助教 (00396938)
• Project Period (FY): 2006 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥77,000,000 (Direct Cost: ¥77,000,000); Fiscal Year 2010: ¥15,400,000 (Direct Cost: ¥15,400,000); Fiscal Year 2009: ¥15,400,000 (Direct Cost: ¥15,400,000); Fiscal Year 2008: ¥15,400,000 (Direct Cost: ¥15,400,000); Fiscal Year 2007: ¥15,400,000 (Direct Cost: ¥15,400,000); Fiscal Year 2006: ¥15,400,000 (Direct Cost: ¥15,400,000)
• Keywords: タペート / miRNA / マイクロアレイ / 雌性配偶体 / 卵 / 助細胞 / ミトコンドリア / イネ / シロイヌナズナ / 卵細胞 / 精細胞 / in vitro受精系 / 多光子レーザー顕微鏡 / ミトコンドリア分裂 / タペート崩壊 / ミトコンドリア融合 / 分裂因子

Research Abstract

(1)タペート崩壊時におけるミトコンドリアの動態
花粉形成過程のシロイヌナズナおよびイネにおいて,タペート細胞が正常な形態のオルガネラおよび細胞膜を保持したまま,葯室内の花粉粒の間にアメーバ状に陥入していくことがわかった.これまで,イネ,シロイヌナズナはともに,他の多くの被子植物と同様にタペート細胞が葯室周縁に留まる分泌型タペートを有していると考えられてきた.しかし本研究の結果から,崩壊に先行して細胞自体が葯室内へ陥入していく侵入型のタペートであることが明らかとなった.またタペート陥入に先だって,減数分裂期の前から細胞壁が消失し始めることが明らかとなった.今後は,花粉の成熟過程における陥入したタペートからの物質輸送について明らかにしたい.
(2)イネ雌性配偶体構成細胞における遺伝子発現プロファイリング
本研究では,イネ雌性配偶体構成細胞の遺伝子発現プロファイリングにより各細胞の機能を解明することを目指し,卵細胞,助細胞中央細胞,反足細胞をそれぞれ生細胞の状態で多数単離し,それぞれの細胞から抽出したRNAを用いてマイクロアレイ解析を行った.その結果,雌性配偶体を構成する細胞の中では助細胞が極めて特異的な発現プロファイルを持つことが明らかとなった.各細胞で特異的に発現量が変化する遺伝子群を本年度報告されたシロイヌナズナのプロファイルと比較すると,イネとの共通性は低かった.また,同様の方法で雌性配偶体構成細胞をそれぞれ単離し,次世代シーケンサを用いて転写産物の配列を網羅的に取得した.現在,得られたデータを解析している.

Research Products

• [Journal Article] Distinct Gene Expression Profiles in Egg and Synergid Cells of Rice as Revealed by Cell Type-specific Microarrays. (2011)
  • Author(s): Ohnishi, T.
  • Journal Title: Plant Physiol. Volume: 155 Pages: 881-891
  • Peer Reviewed
• [Journal Article] MIRO1 influences the morphology and intracellular distribution of mitochondria during embryonic cell division in Arabidopsis (2010)
  • Author(s): Yamaoka, S.
  • Journal Title: Plant Cell Rep. Volume: 30 Pages: 239-244
  • Peer Reviewed
• [Journal Article] Rice expression atlas in reproductive development (2010)
  • Author(s): Fujita, M.
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 2060-2081
  • Peer Reviewed
• [Journal Article] The involvement of a PPR protein of the P subfamily in partial RNA editing of anArabidopsis mitochondrial transcript (2010)
  • Author(s): Doniwa, Y.
  • Journal Title: Gene 454 Pages: 39-46
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis (2010)
  • Author(s): Fujimoto, M.
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 6094-6099
  • Peer Reviewed
• [Journal Article] Studies of mitochondrial morphology and DNA amount in the rice egg cell (2010)
  • Author(s): Takanashi, H.
  • Journal Title: Curr.Genet. 56 Pages: 33-41
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission (2009)
  • Author(s): Fujimono, M.
  • Journal Title: Plant J. 58 Pages: 388-400
  • Peer Reviewed
• [Journal Article] Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5) (2009)
  • Author(s): Nagano, M.
  • Journal Title: Plant J. 58 Pages: 122-134
  • Peer Reviewed
• [Journal Article] Rice-specific mitochondrial iron-regulated gene(MIR)plays an important role in iron homeostasis (2009)
  • Author(s): Ishimaru, Y.
  • Journal Title: Mol.Plant 2 Pages: 1059-1066
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission (2009)
  • Author(s): Fujimoto, M., Arimura, S., Mano, S., Kondo, M., Saito, C., Ueda, T., Nakazono, M., Nakano, A., Nishimura, M., and Tsutsumi, N.
  • Journal Title: Plant Journal 58 Pages: 388-400
• [Journal Article] Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5) (2009)
  • Author(s): Nagano, M., Ihara-Ohori, Y., Imai, H., Inada, N., Fujimoto, M., Tsutsumi, N., Uchimiya, H., and Kawai-Yamada, M.
  • Journal Title: Plant Journal 58 Pages: 122-134
• [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission (2009)
  • Author(s): Fujimoto, M
  • Journal Title: Plant J. (In press)
  • Peer Reviewed
• [Journal Article] Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5) (2009)
  • Author(s): Nagano M
  • Journal Title: Plant J. 58 Pages: 122-134
  • Peer Reviewed
• [Journal Article] Arabidopsis ELONGATED MITOCHONDRIA1 is required for localization of DYNAMIN-RELATED PROTEIN3A to mitochondrial fission sites (2008)
  • Author(s): Arimura, S., Fujimoto, M., Doniwa, Y., Kadoya, N., Nakazono, M., Sakamoto, W., and Tsutsumi, N.
  • Journal Title: Plant Cell 20 Pages: 1555-1566
• [Journal Article] Separated Transcriptomes of Male Gametophyte and Tapetum in Rice: Validity of a Laser Microdissection (LM) Microarray (2008)
  • Author(s): Suwabe, K., Suzuki, G., Takahashi, H., Shiono, K., Endo, M., Yano, K., Fujita, M., Masuko, H., Saito, H., Fujioka, T., Kaneko, F., Kazama, T., Mizuta, Y., Kawagishi-Kobayashi, M., Tsutsumi, N., Kurata, N., Nakazono, M., and Watanabe, M.
  • Journal Title: Plant and Cell Physiology 49 Pages: 1407-1416
  • NAID: 10027333829
• [Journal Article] Various Spatiotemporal Expression Profiles of Anther-Expressed Genes in Rice (2008)
  • Author(s): Hobo, T., Suwabe, K., Aya, K., Suzuki, G., Yano, K., Ishimizu, T., Fujita, M., Kikuchi, S., Hamada, K., Miyano, M., Fujioka, T., Kaneko, F., Kazama, T., Mizuta, Y., Takahashi, H., Shiono, K., Nakazono, M., Tsutsumi, N., Nagamura, Y., Kurata, N., Watanabe, M., and Matsuoka, M.
  • Journal Title: Plant and Cell Physiology 49 Pages: 1417-1428
  • NAID: 10027333889
• [Journal Article] Arabidopsis ELONGATED MITOCHONDRIAI is required for localization of DYNAMIN-RELATED PROTEIN3A to mitochondrial fission sites (2008)
  • Author(s): Arimura S
  • Journal Title: Plant Cell 20 Pages: 1555-1566
  • Peer Reviewed
• [Journal Article] Substitution of the gene for chloroplast RPS16 was assisted by generation of a dual targeting signal (2008)
  • Author(s): Ueda M
  • Journal Title: Mol Biol Evol. 25 Pages: 1566-1575
  • Peer Reviewed
• [Journal Article] Various spatiotemporal expression profiles of anther-expressed genes in rice (2008)
  • Author(s): Hobo T
  • Journal Title: Plant Cell Physiol 49 Pages: 1417-1428
  • Peer Reviewed
• [Journal Article] Separated transcriptomes of male gametophyte and tapetum in rice : validity of a laser microdissection (LM) microarray (2008)
  • Author(s): Suwabe K
  • Journal Title: Plant Cell Physiol 49 Pages: 1407-1406
  • Peer Reviewed
• [Journal Article] Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging (2008)
  • Author(s): Matsushima R
  • Journal Title: Plant Cell Physiol 49 Pages: 1074-1083
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate (2008)
  • Author(s): Fujimoto M
  • Journal Title: Plant Cell Rep. 27 Pages: 1581-1586
  • Peer Reviewed
• [Journal Article] Presence of a latent mitochondrial targeting signal in gene, on mitoch ondrial genome (2008)
  • Author(s): Ueda M
  • Journal Title: Mol. Biol. Evol. 25 Pages: 1791-1793
• [Journal Article] Identiiication of the USUFK7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of iasmonic acid in rice (2008)
  • Author(s): Tani T
  • Journal Title: Planta 227 Pages: 517-526
  • Peer Reviewed
• [Journal Article] Mitochonria use action filaments as rails for fast translocation in Arabidopsis and tobacco cells. (2007)
  • Author(s): Doniwa Y., Arimura SI., Tsutsumi N.
  • Journal Title: Plant Biotech. 24 Pages: 441-447
  • Peer Reviewed
• [Journal Article] lmaging of plant dynamin-related proteins and clatnrin around the plasma membrane by variable incidence angle fluorescence microscopy. (2007)
  • Author(s): Fujimoto M., Arimura SI., Nakazono M., Tsutsumi N.
  • Journal Title: Plant Biotech. 24 Pages: 449-455
  • Peer Reviewed
• [Journal Article] Loss of the rp132 gene from the chloroplast genome and subsequent acquisition of a preexisting transit peptide within the nuclear gene in Ponulus. (2007)
  • Author(s): Ueda M., Fujimoto M., Arimura SI., Murata J., Tsutsumi N., Kadowaki KI.
  • Journal Title: Gene 402 Pages: 51-56
  • Peer Reviewed
• [Journal Article] Single-organelle tracking by two-photon conversion (2007)
  • Author(s): Watanabe, W. et al.
  • Journal Title: Optics Express 15 Pages: 2490-2498
• [Journal Article] Isolation and characterization of the pea cytochrome c oxidase Vb gene (2006)
  • Author(s): Kubo, N. et al.
  • Journal Title: Genome 49 Pages: 1481-1489
• [Journal Article] Different amounts of DNA in each mitochondrion in rice root (2006)
  • Author(s): Takanashi, H. et al.
  • Journal Title: Genes and Genetic Systems 81 Pages: 215-218
• [Journal Article] The mitochondrial fission regulator DRP3B does not regulate cell death in plants (2006)
  • Author(s): Yoshinaga, K. et al.
  • Journal Title: Annals of Botany 97 Pages: 1145-1149
• [Journal Article] Promoter shuffling at a nuclear gene for mitochondrial RPL27. Involvement of interchromosome and subsequent intrachromosome recombinations (2006)
  • Author(s): Ueda, M. et al.
  • Journal Title: Plant Physiology 141 Pages: 702-710
• [Journal Article] Evidence for transit peptide acquisition through duplication and subsequent frameshift mutation of a preexisting protein gene in rice (2006)
  • Author(s): Ueda, M. et al.
  • Journal Title: Molecular Biology and Evolution 23 Pages: 2405-2412