Research on the molecular mechanism of singularity phenomenon in neurological disorders

• Project Area: Singularity biology
• Project/Area Number: 18H05414
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Waseda University
• Principal Investigator: 坂内 博子 早稲田大学, 理工学術院, 教授 (40332340)
• Co-Investigator(Kenkyū-buntansha): 高島 明彦 学習院大学, 理学部, 教授 (00154774) ; 廣島 通夫 国立研究開発法人理化学研究所, 生命機能科学研究センター, 上級研究員 (20392087) ; 松本 弦 長崎大学, 医歯薬学総合研究科(医学系), 講師 (50415303) ; 松田 恵子 慶應義塾大学, 医学部(信濃町), 講師 (40383765)
• Project Period (FY): 2018-06-29 – 2023-03-31
• Project Status: Granted (Fiscal Year 2022)
• Budget Amount: ¥117,780,000 (Direct Cost: ¥90,600,000、Indirect Cost: ¥27,180,000); Fiscal Year 2022: ¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000); Fiscal Year 2021: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000); Fiscal Year 2020: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000); Fiscal Year 2019: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2018: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
• Keywords: シンギュラリティ / タウ / アルツハイマー病 / １分子イメージング / タンパク質凝集 / 光遺伝学 / 脳神経疾患 / プローブ開発 / ゲノム編集 / イメージング / シンギュラリティ生物学 / プローブ

Outline of Annual Research Achievements

今年度は、光遺伝学的にタウの凝集を誘導するツールをゲノム編集法によりノックインしたNeuro2aを用いて、光刺激により誘導されるタウの凝集体の動態と特性の解析を主に行った。免疫蛍光染色法によりタウと微小管をラベルした細胞を超解像顕微鏡法(SIM)により観察したところ、光照射によるTauの集積は最初は微小管に沿って線状におこることがわかった。光刺激後暗環境下で細胞を長期間培養すると、微小管に沿ってできた筋状の集積がいったん解消され、その後粒状の凝集体様構造が生成されることが見出された。生化学的解析によりこの凝集体様の特性を調べたところ、粒状の凝集体様構造はサルコシジル不溶性画分に含まれないこと、主要なリン酸化を受けていないことがわかった。
凝集体様構造ができたNeuro2aの細胞の性質を明らかにするために、全自動イメージング法AISISを用いて、細胞膜上のEGF受容体の動態を解析した。青色光照射細胞でのみ、動きの遅いフラクションに異常がみられたことから、細胞膜環境になんらかの変化が生じたことが示唆された。
本研究の大きな目的の一つは、タウ凝集を検出するためのプローブを作成することである。光遺伝学的にタウの凝集を誘導するツールをノックインした細胞を用いて、光照射細胞においてシグナルが増加するようなタウ凝集プローブ(A01-2班が開発）のスクリーニングを行った。光照射細胞でわずかにシグナル増加するプローブは存在したが、実用化できる水準のシグナル変化を見せるプローブ候補はみつからなかった。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

光遺伝学的にタウの局在をコントロールすることのできるツールにより、人為的にタウを集積させた状況をつくることができた。その凝集体様性質も、超解像顕微鏡法やイメージング、生化学を用いて多角的に解析することができた。

Strategy for Future Research Activity

今年度の研究から、光遺伝学的ツールによる凝集体様以外にもタウの病理を再現するシステムが必要であることが判明した。そのため今後は、タウ凝集シード導入による病理誘導モデルシステムも並行して採用し、タウプローブのスクリーニングもすすめる。また、光遺伝学的ツールにより誘導されるタウ病理をさらに進める条件の検討も行い、超解像イメージング・生化学と多角的に凝集体の性質を解析する。光遺伝学
的ツールおよびプローブ候補をマウス脳へ導入することも目指す。

Research Products

• [Int'l Joint Research] MaxPlank DR(ドイツ)
• [Journal Article] Origin of diverse phosphorylation patterns in the ERBB system (2022)
  • Author(s): Okada Takashi、Miyagi Hiraku、Sako Yasushi、Hiroshima Michio、Mochizuki Atsushi
  • Journal Title: Biophysical Journal Volume: 121 Pages: 470-480
  • DOI: 10.1016/j.bpj.2021.12.031
  • NAID: 120007187502
  • Peer Reviewed / Open Access
• [Journal Article] Heterotrimeric Gq proteins?act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias (2022)
  • Author(s): Kawakami Kouki、Yanagawa Masataka、Hiratsuka Suzune、Yoshida Misaki、Ono Yuki、Hiroshima Michio、Ueda Masahiro、Aoki Junken、Sako Yasushi、Inoue Asuka
  • Journal Title: Nature Communications Volume: 13 Pages: 1-16
  • DOI: 10.1038/s41467-022-28056-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SRC kinase activator CDCP1 promotes hepatocyte growth factor-induced cell migration/invasion of a subset of breast cancer cells (2022)
  • Author(s): Kawase Naoyuki、Sugihara Atsuya、Kajiwara Kentaro、Hiroshima Michio、Akamatsu Kanako、Nada Shigeyuki、Matsumoto Kunio、Ueda Masahiro、Okada Masato
  • Journal Title: J Biol Chem Volume: 298 Pages: 101630-101630
  • DOI: 10.1016/j.jbc.2022.101630
  • Peer Reviewed / Open Access
• [Journal Article] Dendritic distribution of CDK5 mRNA and p35 mRNA, and a glutamate-responsive increase of CDK5/p25 complex contribute to tau hyperphosphorylation (2022)
  • Author(s): Tanaka Toru、Ohashi Sachiyo、Takashima Akihiko、Kobayashi Shunsuke
  • Journal Title: Biochimica et Biophysica Acta - General Subjects Volume: 1866 Pages: 130135-130135
  • DOI: 10.1016/j.bbagen.2022.130135
  • Peer Reviewed
• [Journal Article] Administration of mucuna beans (Mucuna pruriences (L.) DC. var. utilis) improves cognition and neuropathology of 3?×?Tg-AD mice (2022)
  • Author(s): Konishi Fumiko、Furusho Tadasu、Soeda Yoshiyuki、Yamauchi Jun、Kobayashi Shoko、Ito Masatoshi、Araki Takuma、Kogure Sarasa、Takashima Akihiko、Takekoshi Susumu
  • Journal Title: Sci Rep Volume: 12 Pages: 996-996
  • DOI: 10.1038/s41598-022-04777-z
  • Peer Reviewed / Open Access
• [Journal Article] DNA‐Based Synthetic Growth Factor Surrogates with Fine‐Tuned Agonism** (2021)
  • Author(s): Akiyama Momoko、Ueki Ryosuke、Yanagawa Masataka、Abe Mitsuhiro、Hiroshima Michio、Sako Yasushi、Sando Shinsuke
  • Journal Title: Angewandte Chemie International Edition Volume: 60 Pages: 22745-22752
  • DOI: 10.1002/anie.202105314
  • Peer Reviewed / Open Access
• [Journal Article] Comparative Analysis of Single-Molecule Dynamics of TRPV1 and TRPV4 Channels in Living Cells (2021)
  • Author(s): Kuwashima Yutaro、Yanagawa Masataka、Abe Mitsuhiro、Hiroshima Michio、Ueda Masahiro、Arita Makoto、Sako Yasushi
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Pages: 8473-8473
  • DOI: 10.3390/ijms22168473
  • Peer Reviewed / Open Access
• [Journal Article] Deposition of Phosphorylated α-Synuclein and Activation of GSK-3β and PP2A in the PS19 Mouse Model of Tauopathy (2021)
  • Author(s): Takaichi Yuta、Chambers James K、Ano Yasuhisa、Takashima Akihiko、Nakayama Hiroyuki、Uchida Kazuyuki
  • Journal Title: Journal of Neuropathology & Experimental Neurology Volume: 80 Pages: 731-740
  • DOI: 10.1093/jnen/nlab054
  • Peer Reviewed
• [Journal Article] Disulfide bond formation in microtubule-associated tau protein promotes tau accumulation and toxicity <i>in vivo</i> (2021)
  • Author(s): Saito Taro、Chiku Tomoki、Oka Mikiko、Wada-Kakuda Satoko、Nobuhara Mika、Oba Toshiya、Shinno Kanako、Abe Saori、Asada Akiko、Sumioka Akio、Takashima Akihiko、Miyasaka Tomohiro、Ando Kanae
  • Journal Title: Human Molecular Genetics Volume: 30 Pages: 1955-1967
  • DOI: 10.1093/hmg/ddab162
  • Peer Reviewed / Open Access
• [Journal Article] Essential roles of plexin-B3(+) oligodendrocyte precursor cells in the pathogenesis of Alzheimer's disease. (2021)
  • Author(s): Nihonmatsu-Kikuchi N, Yu XJ, Matsuda Y, Ozawa N, Ito T, Satou K, Kaname T, Iwasaki Y, Akagi A, Yoshida M, Toru S, Hirokawa K, Takashima A, Hasegawa M, Uchihara T, Tatebayashi Y
  • Journal Title: Communications Biology Volume: 4 Pages: 870-870
  • DOI: 10.1038/s42003-021-02404-7
  • Peer Reviewed / Open Access
• [Journal Article] β-Lactolin Reduces Age-Related Inflammation and Cognitive Decline (2021)
  • Author(s): Ano Yasuhisa、Ohya Rena、Takashima Akihiko、Uchida Kazuyuki、Nakayama Hiroyuki
  • Journal Title: Frontiers in Nutrition Volume: 8 Pages: 724134-724134
  • DOI: 10.3389/fnut.2021.724134
  • Peer Reviewed / Open Access
• [Journal Article] Cell-to-cell diversification in ERBB-RAS-MAPK signal transduction that produces cell-type specific growth factor responses (2021)
  • Author(s): Miyagi Hiraku、Hiroshima Michio、Sako Yasushi
  • Journal Title: Biosystems Volume: 199 Pages: 104293-104293
  • DOI: 10.1016/j.biosystems.2020.104293
  • Peer Reviewed / Open Access
• [Journal Article] In-Cell Single-Molecule Analysis of Molecular State and Reaction Kinetics Coupling (2021)
  • Author(s): Hiroshima Michio、Sako Yasushi
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 1310 Pages: 59-80
  • DOI: 10.1007/978-981-33-6064-8_3
  • Peer Reviewed
• [Journal Article] Inhibitory synaptic transmission tuned by Ca(2+) and glutamate through the control of GABA(A) R lateral diffusion dynamics (2020)
  • Author(s): Bannai Hiroko、Niwa Fumihiro、Sakuragi Shigeo、Mikoshiba Katsuhiko
  • Journal Title: Development, Growth & Differentiation Volume: 62 Pages: 398-406
  • DOI: 10.1111/dgd.12667
  • Peer Reviewed / Open Access
• [Journal Article] Large scale single-molecule imaging aided by artificial intelligence (2020)
  • Author(s): Hiroshima, M., Yasui, M., and Ueda, M.
  • Journal Title: Microscopy Volume: 69 Pages: 69-78
  • DOI: 10.1093/jmicro/dfz116
  • Peer Reviewed / Open Access
• [Journal Article] β-Lactolin, a Whey-Derived Lacto-Tetrapeptide, Prevents Alzheimer’s Disease Pathologies and Cognitive Decline (2020)
  • Author(s): Ano Yasuhisa、Ohya Rena、Takaichi Yuta、Washinuma Terukatsu、Uchida Kazuyuki、Takashima Akihiko、Nakayama Hiroyuki
  • Journal Title: Journal of Alzheimer's Disease Volume: 73 Pages: 1331-1342
  • DOI: 10.3233/jad-190997
  • Peer Reviewed
• [Journal Article] Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single-Particle Tracking (2020)
  • Author(s): Bannai Hiroko、Inoue Takafumi、Hirose Matsumi、Niwa Fumihiro、Mikoshiba Katsuhiko
  • Journal Title: Neuromethods Volume: 154 Pages: 131-155
  • DOI: 10.1007/978-1-0716-0532-5_7
  • Peer Reviewed
• [Journal Article] AIにより実現した細胞内1分子動態の大規模網羅的イメージング (2020)
  • Author(s): 廣島通夫
  • Journal Title: 日本化学会情報化学部会誌（CICSJ Bulletin） Volume: 38 Pages: 12-15
  • NAID: 130007960374
• [Journal Article] New Insights Into Drug Discovery Targeting Tau Protein (2020)
  • Author(s): Yoshiyuki Soeda, Akihiko Takashima
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 13 Pages: 590896-590896
  • DOI: 10.3389/fnmol.2020.590896
  • Peer Reviewed / Open Access
• [Journal Article] Hop bitter acids containing a β-carbonyl moiety prevent inflammation-induced cognitive decline via the vagus nerve and noradrenergic system (2020)
  • Author(s): Ano Yasuhisa、Ohya Rena、Yamazaki Takahiro、Takahashi Chika、Taniguchi Yoshimasa、Kondo Keiji、Takashima Akihiko、Uchida Kazuyuki、Nakayama Hiroyuki
  • Journal Title: Scientific Reports Volume: 10 Pages: 20028-20028
  • DOI: 10.1038/s41598-020-77034-w
  • Peer Reviewed / Open Access
• [Journal Article] Glutamate-responsive translation of dendritic GSK3β mRNA triggers a cycle for amplification of reactivated preexisting GSK3β that is indispensable for tau hyperphosphorylation (2020)
  • Author(s): Tanaka Toru、Ohashi Sachiyo、Takashima Akihiko、Kobayashi Shunsuke
  • Journal Title: Neurochemistry International Volume: 139 Pages: 104808-104808
  • DOI: 10.1016/j.neuint.2020.104808
  • Peer Reviewed
• [Journal Article] Phosphorylation and oligomerization of α-synuclein associated with GSK-3β activation in the rTg4510 mouse model of tauopathy (2020)
  • Author(s): Takaichi Yuta、Chambers James K.、Inoue Hiroyuki、Ano Yasuhisa、Takashima Akihiko、Nakayama Hiroyuki、Uchida Kazuyuki
  • Journal Title: Acta Neuropathologica Communications Volume: 8 Pages: 86-86
  • DOI: 10.1186/s40478-020-00969-8
  • NAID: 120006902252
  • Peer Reviewed / Open Access
• [Journal Article] Distribution of endogenous normal tau in the mouse brain. (2020)
  • Author(s): Kubo A, Misonou H, Matsuyama M, Nomori A, Wada-Kakuda S, Takashima A, Kawata M, Murayama S, Ihara Y, Miyasaka T
  • Journal Title: Journal of Comparative Neurology Volume: 527 Pages: 985-998
  • DOI: 10.1002/cne.24577
  • Peer Reviewed / Open Access
• [Journal Article] Dissection of Local Ca²⁺ Signals in Cultured Cells by Membrane-targeted Ca²⁺ Indicators (2019)
  • Author(s): Bannai Hiroko、Hirose Matsumi、Niwa Fumihiro、Mikoshiba Katsuhiko
  • Journal Title: J. Vis. Exp. Volume: 145
  • DOI: 10.3791/59246
  • Peer Reviewed / Open Access
• [Journal Article] Methylene Blue Inhibits Formation of Tau Fibrils but not of Granular Tau Oligomers: A Plausible Key to Understanding Failure of a Clinical Trial for Alzheimer’s Disease (2019)
  • Author(s): Soeda Yoshiyuki、Saito Marino、Maeda Sumihiro、Ishida Kohki、Nakamura Akira、Kojima Shuichi、Takashima Akihiko
  • Journal Title: Journal of Alzheimer's Disease. Volume: 68 Pages: 1677-1686
  • DOI: 10.3233/jad-181001
  • Peer Reviewed
• [Journal Article] Tryptophan-related Dipeptides in Fermented Dairy Products Suppress Microglial Activation and Prevent Cognitive Decline (2019)
  • Author(s): Ano Y, Yoshino Y, Kutsukake T, Ohya R, Fukuda T, Uchida K, Takashima A, Nakayama H
  • Journal Title: Aging Volume: 11 Pages: 2949-2967
  • DOI: 10.18632/aging.101909
  • Peer Reviewed
• [Journal Article] Dysregulation of autophagy and stress granule-related proteins in stress-driven Tau pathology (2019)
  • Author(s): Silva Joana Margarida、Rodrigues Sara、Sampaio-Marques B、Gomes Patr?cia、Neves-Carvalho Andreia、Dioli Chrysoula、Soares-Cunha Carina、Mazuik Brandon F、Takashima Akihiko、Ludovico Paula、Wolozin Benjamin、Sousa Nuno、Sotiropoulos Ioannis
  • Journal Title: Cell Death and Differentiation Volume: - Pages: 1411-1427
  • DOI: 10.1038/s41418-018-0217-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Enhanced Tau Protein Translation by Hyper-Excitation (2019)
  • Author(s): Kobayashi S, Tanaka T, Soeda Y, Takashima A.
  • Journal Title: Front Aging Neurosci, Volume: 11 Pages: 322-322
  • DOI: 10.3389/fnagi.2019.00322
  • Peer Reviewed
• [Journal Article] Pathological Progression Induced by the Frontotemporal Dementia-Associated R406W Tau Mutation in Patient-Derived iPSCs (2019)
  • Author(s): Nakamura Mari、Shiozawa Seiji、Tsuboi Daisuke、Amano Mutsuki、Watanabe Hirotaka、Maeda Sumihiro、Kimura Taeko、Yoshimatsu Sho、Kisa Fumihiko、Karch Celeste M.、Miyasaka Tomohiro、Takashima Akihiko、Sahara Naruhiko、Hisanaga Shin-ichi、Ikeuchi Takeshi、Kaibuchi Kozo、Okano Hideyuki
  • Journal Title: Stem Cell Reports Volume: 13 Pages: 684-699
  • DOI: 10.1016/j.stemcr.2019.08.011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tau Oligomers (2019)
  • Author(s): Maeda S, Takashima A.
  • Journal Title: Adv Exp Med Biol . Volume: 1184 Pages: 373-380
  • DOI: 10.1007/978-981-32-9358-8_27
  • Peer Reviewed
• [Journal Article] Molecular membrane dynamics: Insights into synaptic function and neuropathological disease (2018)
  • Author(s): Bannai Hiroko
  • Journal Title: Neurosci Res Volume: 129 Pages: 47-56
  • DOI: 10.1016/j.neures.2017.07.007
  • Peer Reviewed
• [Journal Article] Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters (2018)
  • Author(s): Hiroshima Michio、Pack Chan-gi、Kaizu Kazunari、Takahashi Koichi、Ueda Masahiro、Sako Yasushi
  • Journal Title: Journal of Molecular Biology Volume: 430 Pages: 1386-1401
  • DOI: 10.1016/j.jmb.2018.02.018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Single-molecule diffusion-based estimation of ligand effects on G protein?coupled receptors (2018)
  • Author(s): Yanagawa Masataka、Hiroshima Michio、Togashi Yuichi、Abe Mitsuhiro、Yamashita Takahiro、Shichida Yoshinori、Murata Masayuki、Ueda Masahiro、Sako Yasushi
  • Journal Title: Sci. Signal. Volume: 11 Pages: 1-16
  • DOI: 10.1126/scisignal.aao1917
  • Peer Reviewed / Open Access
• [Journal Article] Automated single-molecule imaging in living cells (2018)
  • Author(s): Yasui Masato、Hiroshima Michio、Kozuka Jun、Sako Yasushi、Ueda Masahiro
  • Journal Title: Nature Communications Volume: 9 Pages: 1-11
  • DOI: 10.1038/s41467-018-05524-7
  • Peer Reviewed / Open Access
• [Journal Article] Shift in Conformational Equilibrium Induces Constitutive Activity of G-Protein-Coupled Receptor, Rhodopsin. (2018)
  • Author(s): Maeda R, Hiroshima M, Yamashita T, Wada A, Sako Y, Shichida Y, Imamoto Y.
  • Journal Title: J Phys Chem B Volume: 122 Pages: 4838-4843
  • DOI: 10.1021/acs.jpcb.8b02819
  • NAID: 120006708207
  • Peer Reviewed / Open Access
• [Presentation] アルツハイマー病モデル細胞における1分子イメージング; ― 全自動1分子イメージングによる大規模画像データ取得に向けて ― (2022)
  • Author(s): 坂内博子・廣島通夫
  • Organizer: 第127回日本解剖学会総会・全国学術集会
  • Invited
• [Presentation] Approach to Alzheimer’s disease by singularity biology (2021)
  • Author(s): Hiroko Bannai
  • Organizer: Pacifichem2021
  • Int'l Joint Research / Invited
• [Presentation] Single molecule imaging for understanding brain function, neurological disorders, and drug action mechanism (2021)
  • Author(s): Hiroko Bannai
  • Organizer: 14th International Symposium on Nanomedicine
  • Int'l Joint Research / Invited
• [Presentation] Synaptic function revealed by single-molecule imaging (2021)
  • Author(s): Hiroko Bannai
  • Organizer: 14th Canadian Neuroscience Meeting
  • Int'l Joint Research / Invited
• [Presentation] １分子イメージングによる脳神経疾患へのアプローチ (2021)
  • Author(s): 坂内博子
  • Organizer: 第64回日本神経化学学会大会
  • Invited
• [Presentation] １分子イメージングから読み解く神経生理・病理 (2021)
  • Author(s): 坂内博子
  • Organizer: 第13回光塾
  • Invited
• [Presentation] What molecular behavior tells us about brain? -Physiology and pathology of neurons revealed by single-molecule imaging (2021)
  • Author(s): Hiroko Bannai
  • Organizer: The 1st ASHBi SignAC Workshop 2021
  • Int'l Joint Research / Invited
• [Presentation] １分子イメージングで読み解く脳神経生理と病理 (2021)
  • Author(s): 坂内博子
  • Organizer: 早稲田大学NCNP合同セミナー
  • Invited
• [Presentation] 分子動態が語る神経生理・病理 (2021)
  • Author(s): 坂内博子
  • Organizer: 東京女子医科大学・早稲田大学_研究交流会
  • Invited
• [Presentation] タウオリゴマーの人為的誘導の可能性 (2021)
  • Author(s): 坂内 博子、亀崎貴也、吉村 英哲、添田義行、高島明彦
  • Organizer: 新学術領域「シンギュラリティ生物学」第４回領域会議
• [Presentation] Novel insight into neurological disorders revealed single-molecule imaging (2021)
  • Author(s): Hiroko Bannai
  • Organizer: Joint Symposium between Waseda University and the University of Bonn
  • Int'l Joint Research / Invited
• [Presentation] AMPA受容体, GABAA受容体に対する細胞外タウタンパク質の影響 (2021)
  • Author(s): 町田彩乃、櫻木繁雄、添田義行、高島明彦、坂内博子
  • Organizer: 新学術領域「シンギュラリティ生物学」第４回領域会議
• [Presentation] 全自動１分子解析による毒性タウ分子の伝播メカニズムの解明に向けて (2021)
  • Author(s): 廣島通夫
  • Organizer: 新学術領域「シンギュラリティ生物学」第４回領域会議
• [Presentation] 光遺伝学的手法を用いたタウ凝集体形成 (2021)
  • Author(s): 添田義行、吉村 英哲、坂内博子、高島明彦
  • Organizer: 新学術領域「シンギュラリティ生物学」第４回領域会議
• [Presentation] Pharmacological Application of Automated Single-molecule Analysis for EGFR Cancerous Mutants (2020)
  • Author(s): 廣島通夫, 渡邉大介, 上田昌宏.
  • Organizer: 第58回日本生物物理学会年会
• [Presentation] 細胞内 1 分子イメージングの自動化と細胞内シグナル伝達への適用 (2020)
  • Author(s): 廣島通夫, 上田 昌宏
  • Organizer: CBI学会2020大会
  • Invited
• [Presentation] 超高齢社会を考えるⅣ ＜超高齢社会を支える技術と倫理＞ (2020)
  • Author(s): 高島明彦
  • Organizer: 第８回学習院大学ブランディング・シンポジウム（第27回生命科学シンポジウム）
  • Invited
• [Presentation] 認知症最初期に起こる嗅内野機能低下を検出する VR-ゴーグルの開発 (2020)
  • Author(s): 添田義行、笠井淳史、源田公平、甲斐英隆 、高島明彦
  • Organizer: 第39回日本認知症学会学術集会
• [Presentation] グルタミン酸刺激に応じた樹状突起GSK3βの活性化とタウの過剰リン酸化 (2020)
  • Author(s): 田中融、大橋祥世、高島明彦、小林俊亮
  • Organizer: 第43回日本分子生物学会
• [Presentation] Physiology and pathology of brains revealed by single molecule imaging (2019)
  • Author(s): Hiroko Bannai
  • Organizer: 17th Chinese Biophysics Congress
  • Int'l Joint Research / Invited
• [Presentation] Approach to Alzheimer’s disease by Singularity Biology (2019)
  • Author(s): Hiroko Bannai
  • Organizer: ICSB 2019 satellite workshop "Singularity Biology"
  • Int'l Joint Research / Invited
• [Presentation] Physiology and pathology of brains revealed by single molecule imaging (2019)
  • Author(s): Hiroko Bannai
  • Organizer: OIST Joint Minisymposium with the 16th International Membrane Research Forum
  • Int'l Joint Research / Invited
• [Presentation] Approach to Alzheimer’s disease by Singularity Biology (2019)
  • Author(s): Hiroko Bannai
  • Organizer: 13th International Symposium on Nanomedicine
  • Int'l Joint Research / Invited
• [Presentation] 1分子イメージングで読み解くアルツハイマー病のシンギュラリティ」 (2019)
  • Author(s): 坂内博子、廣島通夫
  • Organizer: 第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会 合同年次大会 シンポジウム
  • Invited
• [Presentation] Approach to neurodegenerative disease by singularity biology (2019)
  • Author(s): Hiroko Bannai
  • Organizer: Neuro2019 シンポジウム
  • Invited
• [Presentation] １分子イメージングで読み解く脳の生理と病理 (2019)
  • Author(s): 坂内博子
  • Organizer: 和光-精神神経懇話会
  • Invited
• [Presentation] １分子イメージングから読み解くタウの生理機能 (2019)
  • Author(s): 坂内博子
  • Organizer: タウ研究会
  • Invited
• [Presentation] １分子解析による生理的タウの新機能の発見 (2019)
  • Author(s): 坂内博子、金谷美沙、高島明彦
  • Organizer: 第92回日本生化学会大会
  • Invited
• [Presentation] What singularity biology can do for understanding Alzheimer’s disease (2019)
  • Author(s): Hiroko Bannai, Michio Hiroshima, Yoshiyuki Soeda, Akihiko Takashima
  • Organizer: 第57回生物物理学会年会シンポジウム
  • Invited
• [Presentation] 全自動イメージングにより実現した網羅的1分子解析 (2019)
  • Author(s): 廣島通夫
  • Organizer: 第44回レーザ顕微鏡研究会＆シンポジウム
  • Invited
• [Presentation] 全自動化 1 分子イメージングによる大規模計測 (2019)
  • Author(s): 廣島通夫
  • Organizer: CBI学会2019年大会
  • Invited
• [Presentation] Regulation of Downstream Signaling by Clusters of Epidermal Growth Factor Receptor (2019)
  • Author(s): Michio Hiroshima
  • Organizer: 第57回日本生物物理学会年会
• [Presentation] カテコール骨格を持つ低分子化合物のタウ凝集阻害作用 (2019)
  • Author(s): 添田義行 石村結麻 斉藤万梨乃 高島明彦
  • Organizer: 第38回日本認知症学会学術集会
• [Presentation] 植物由来抽出物を使ったタウ凝集阻害剤のスクリーニング (2019)
  • Author(s): 阿部 将志, 添田 義行, 楊 金緯, 立﨑 仁, 高島 明彦
  • Organizer: 第38回日本認知症学会学術集会
• [Presentation] Deciphering the cell individuality of a cell by single molecule analysis of membrane molecule dynamics (2018)
  • Author(s): Hiroko Bannai
  • Organizer: MPFI-JST(PRESTO) Joint Workshop on Neuroscience and Single Cell Analysis
  • Int'l Joint Research / Invited
• [Presentation] Approach to role of physiological Tau protein by single molecule analysis of membrane molecule dynamics (2018)
  • Author(s): Hiroko Bannai, Misa Kanatani, Sumihiro Maeda, Matsumi Hirose, Akihiko Takashima, Katsuhiko Mikoshiba
  • Organizer: 12th International Symposium on NanoMedicine
  • Int'l Joint Research / Invited
• [Presentation] Approach to neurodegenerative disease by singularity biology (2018)
  • Author(s): Hiroko Bannai
  • Organizer: 第56回日本生物物理学会年会
  • Invited
• [Presentation] In Cell Automated Single-molecule Analysis and Its Extensive Applications (2018)
  • Author(s): 廣島通夫
  • Organizer: 第56回日本生物物理学会年会，シンポジウム「1分子計測に立脚した新しいバイオ分析の潮流」
  • Invited
• [Book] Tau Biology (2019)
  • Author(s): Takashima A, Wolozin B, Buee L.
  • Total Pages: 416
  • Publisher: Springer Nature
  • ISBN: 9789813293588