バクテリオファージに学ぶ発動分子システムの創成

• Project Area: Molecular Engine: Design of Autonomous Functions through Energy Conversion
• Project/Area Number: 18H05421
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: 上野 隆史 東京工業大学, 生命理工学院, 教授 (70332179)
• Co-Investigator(Kenkyū-buntansha): 金丸 周司 東京工業大学, 生命理工学院, 助教 (50376951)
• Project Period (FY): 2018-06-29 – 2023-03-31
• Project Status: Granted (Fiscal Year 2022)
• Budget Amount: ¥72,930,000 (Direct Cost: ¥56,100,000、Indirect Cost: ¥16,830,000); Fiscal Year 2022: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000); Fiscal Year 2021: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2020: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2019: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2018: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: T４ファージ / タンパク質集合体 / HSAFM / ソフトマター / 生体超分子 / 分子機械 / 高速原子間力顕微鏡 / バクテリオファージ / 分子針 / 自己集合 / 超分子化学

Outline of Annual Research Achievements

生命環境では、様々なタンパク質が特異な集合構造を形成し、自発的に機動する分子機関を構築されている。本研究では、代表者が開発した、T4ファージ由来の全長15nmのタンパク質針を基盤とし、新しい分子機関の合成をめざした。具体的には、分子機関としてのタンパク質針精密設計として、タンパク質針の結晶構造やHSAFMの実験結果と分子動力学計算を元に、分子機関としての機能を精密に制御したタンパク質針を構築し、タンパク質針をユニットとしてタンパク質針を階層的に集積し、アクティブマターとしての新しい機能の発現を目指した。高速AFMの実験結果を数理モデルを用いた解析により理解することによって、理論計算のモデルを構築し新たな集合体形成のメカニズム解明へとつなげることができた。さらに、これらの結果を踏まえ、タンパク質針の集団運動の制御に向けた精密分子設計の指針ができた。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

高速AFMの実験結果を数理モデルを用いた解析により理解することによって、理論計算のモデルを構築することができたため。

Strategy for Future Research Activity

新たな集合体形成のメカニズム解明から、タンパク質針の集団運動の制御に向けた精密分子設計をすすめる。

Research Products

• [Int'l Joint Research] 清華大学(中国)
• [Int'l Joint Research] TATA基礎科学研究所(インド)
• [Int'l Joint Research] 南洋理工大学(シンガポール)
• [Int'l Joint Research] 清華大学(中国)
• [Int'l Joint Research] マサチューセッツ工科大学(米国)
• [Int'l Joint Research] TATA基礎科学研究所(インド)
• [Int'l Joint Research] 清華大学(中国)
• [Int'l Joint Research] KAIST(韓国)
• [Int'l Joint Research] NTU(シンガポール)
• [Journal Article] Dynamic Behavior of Cargo Proteins Regulated by Linker Peptides on a Protein Needle Scaffold (2022)
  • Author(s): Nguyen Que D.、Kikuchi Kosuke、Kojima Mariko、Ueno Takafumi
  • Journal Title: Chemistry Letters Volume: 51 Issue: 1 Pages: 73-76
  • DOI: 10.1246/cl.210599
  • NAID: 130008137585
  • ISSN: 0366-7022, 1348-0715
  • Year and Date: 2022-01-05
  • Peer Reviewed
• [Journal Article] Importance of the Subunit?Subunit Interface in Ferritin Disassembly: A Molecular Dynamics Study (2022)
  • Author(s): Li Zhipeng、Maity Basudev、Hishikawa Yuki、Ueno Takafumi、Lu Diannan
  • Journal Title: Langmuir Volume: 38 Issue: 3 Pages: 1106-1113
  • DOI: 10.1021/acs.langmuir.1c02753
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Engineering of protein crystals for use as solid biomaterials (2022)
  • Author(s): Kojima Mariko、Abe Satoshi、Ueno Takafumi
  • Journal Title: Biomaterials Science Volume: 10 Issue: 2 Pages: 354-367
  • DOI: 10.1039/d1bm01752g
  • Peer Reviewed
• [Journal Article] Protein Needles Designed to Self‐Assemble through Needle Tip Engineering (2022)
  • Author(s): Kikuchi Kosuke、Fukuyama Tatsuya、Uchihashi Takayuki、Furuta Tadaomi、Maeda Yusuke T.、Ueno Takafumi
  • Journal Title: Small Volume: 18 Issue: 10 Pages: 2106401-2106401
  • DOI: 10.1002/smll.202106401
  • Peer Reviewed
• [Journal Article] Design of a gold clustering site in an engineered apo-ferritin cage (2022)
  • Author(s): Lu Chenlin、Maity Basudev、Peng Xue、Ito Nozomi、Abe Satoshi、Sheng Xiang、Ueno Takafumi、Lu Diannan
  • Journal Title: Communications Chemistry Volume: 5 Issue: 1 Pages: 1-11
  • DOI: 10.1038/s42004-022-00651-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Controlled Uptake of an Iridium Complex inside Engineered apo‐Ferritin Nanocages: Study of Structure and Catalysis** (2022)
  • Author(s): Taher Mohd、Maity Basudev、Nakane Taiki、Abe Satoshi、Ueno Takafumi、Mazumdar Shyamalava
  • Journal Title: Angewandte Chemie International Edition Volume: 61 Issue: 13
  • DOI: 10.1002/anie.202116623
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Solubility enhancement of poorly water soluble domperidone by complexation with the large ring cyclodextrin (2021)
  • Author(s): Ismail Abbas、Kerdpol Khanittha、Rungrotmongkol Thanyada、Tananuwong Kanitha、Ueno Takafumi、Ekasit Sanong、Muangsin Nongnuj、Krusong Kuakarun
  • Journal Title: International Journal of Pharmaceutics Volume: 606 Pages: 120909-120909
  • DOI: 10.1016/j.ijpharm.2021.120909
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Design of in‐cell protein crystal for environmentally responsive construction of a supramolecular filament (2021)
  • Author(s): Abe Satoshi、Pham Thuc Toan、Negishi Hashiru、Yamashita Keitaro、Hirata Kunio、Ueno Takafumi
  • Journal Title: Angewandte Chemie International Edition Volume: - Issue: 22 Pages: 12341-12345
  • DOI: 10.1002/anie.202102039
  • Peer Reviewed
• [Journal Article] The Versatile Manipulations of Self-Assembled Proteins in Vaccine Design (2021)
  • Author(s): Nguyen Que Dan、Kikuchi Kosuke、Maity Basudev、Ueno Takafumi
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 4 Pages: 1934-1934
  • DOI: 10.3390/ijms22041934
  • Peer Reviewed
• [Journal Article] In-Cell Engineering of Protein Crystals with Nanoporous Structures for Promoting Cascade Reactions (2021)
  • Author(s): Nguyen Tien K.、Abe Satoshi、Kasamatsu Makoto、Maity Basudev、Yamashita Keitaro、Hirata Kunio、Kojima Mariko、Ueno Takafumi
  • Journal Title: ACS Applied Nano Materials Volume: 4 Issue: 2 Pages: 1672-1681
  • DOI: 10.1021/acsanm.0c03129
  • Peer Reviewed
• [Journal Article] Design of Multinuclear Gold Binding Site at the Two-fold Symmetric Interface of the Ferritin Cage (2020)
  • Author(s): Yuki Hishikawa,Basudev Maity,Nozomi Ito,Satoshi Abe,Diannan Lu,Takafumi Ueno.
  • Journal Title: Chemistry Letters Volume: 49 Issue: 7 Pages: 840-844
  • DOI: 10.1246/cl.200217
  • NAID: 130007872571
  • ISSN: 0366-7022, 1348-0715
  • Year and Date: 2020-07-05
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Single-molecule level dynamic observation of disassembly of the apo-ferritin cage in solution (2020)
  • Author(s): Maity Basudev、Li Zhipeng、Niwase Kento、Ganser Christian、Furuta Tadaomi、Uchihashi Takayuki、Lu Diannan、Ueno Takafumi
  • Journal Title: Physical Chemistry Chemical Physics Volume: 22 Issue: 33 Pages: 18562-18572
  • DOI: 10.1039/d0cp02069a
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy (2020)
  • Author(s): Ueno Takafumi、Niwase Kento、Tsubokawa Daisho、Kikuchi Kosuke、Takai Natsumi、Furuta Tadaomi、Kawano Ryuji、Uchihashi Takayuki
  • Journal Title: Nanoscale Volume: in press Issue: 15 Pages: 8166-8173
  • DOI: 10.1039/d0nr01121e
  • Peer Reviewed
• [Journal Article] Site-Selective Protein Chemical Modification of Exposed Tyrosine Residues Using Tyrosine Click Reaction (2020)
  • Author(s): Sato Shinichi、Matsumura Masaki、Kadonosono Tetsuya、Abe Satoshi、Ueno Takafumi、Ueda Hiroshi、Nakamura Hiroyuki
  • Journal Title: Bioconjugate Chemistry Volume: in press Issue: 5 Pages: 1417-1424
  • DOI: 10.1021/acs.bioconjchem.0c00120
  • Peer Reviewed
• [Journal Article] Coordination design of cadmium ions at the 4-fold axis channel of the apo-ferritin cage (2019)
  • Author(s): Abe Satoshi、Ito Nozomi、Maity Basudev、Lu Chenlin、Lu Diannan、Ueno Takafumi
  • Journal Title: Dalton Transactions Volume: 印刷中 Issue: 26 Pages: 9759-9764
  • DOI: 10.1039/c9dt00609e
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Recent progresses in the accumulation of metal ions into the apo-ferritin cage: Experimental and theoretical perspectives (2019)
  • Author(s): Maity Basudev、Hishikawa Yuki、Lu Diannan、Ueno Takafumi
  • Journal Title: Polyhedron Volume: 印刷中 Pages: 104-111
  • DOI: 10.1016/j.poly.2019.03.048
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Engineering of protein crystals for development of bionanomaterials (2019)
  • Author(s): T. K. Nguyen, T. T. Pham, and T Ueno
  • Journal Title: Jpn. J. Appl. Phys. Volume: 印刷中
  • NAID: 210000156508
  • Peer Reviewed
• [Journal Article] Encapsulation of biomacromolecules by soaking and co-crystallization into porous protein crystals of hemocyanin (2019)
  • Author(s): Tsubasa Hashimoto, Yuxin Ye, Asuka Matsuno, Yuki Ohnishi, Akira Kitamura, Masataka Kinjo, Satoshi Abe, Takafumi Uenod, Min Yao, Tomohisa Ogawa, Takashi Matsuia, Yoshikazu Tanaka
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 509 Issue: 2 Pages: 577-584
  • DOI: 10.1016/j.bbrc.2018.12.096
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Construction of supramolecular nanotubes from protein crystals (2019)
  • Author(s): Nguyen Tien Khanh、Negishi Hashiru、Abe Satoshi、Ueno Takafumi
  • Journal Title: Chemical Science Volume: 10 Issue: 4 Pages: 1046-1051
  • DOI: 10.1039/c8sc04167a
  • Peer Reviewed
• [Journal Article] Photoinduced in Vivo Magnetic Resonance Imaging (MRI) with Rapid CO Release from an MnCO-Protein Needle Composite (2018)
  • Author(s): Hyodo Fuminori、Sho Takeya、Maity Basudev、Fujita Kenta、Tachibana Yoko、Akashi Satoko、Mano Megumi、Hishikawa Yuki、Matsuo Masayuki、Ueno Takafumi
  • Journal Title: Chemistry - A European Journal Volume: 24 Issue: 45 Pages: 11578-11583
  • DOI: 10.1002/chem.201802445
  • Peer Reviewed
• [Journal Article] Raman spectroscopy insight into Norovirus encapsulation in Bombyx mori cypovirus cubic microcrystals (2018)
  • Author(s): Mori Hajime、Oda Naoki、Abe Satoshi、Ueno Takafumi、Zhu Wenliang、Pernstich Chris、Pezzotti Giuseppe
  • Journal Title: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Volume: 203 Pages: 19-30
  • DOI: 10.1016/j.saa.2018.05.066
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Photocatalytic hydrogen evolution systems constructed in cross-linked porous protein crystals (2018)
  • Author(s): Tabe Hiroyasu、Takahashi Hikaru、Shimoi Takuya、Abe Satoshi、Ueno Takafumi、Yamada Yusuke
  • Journal Title: Applied Catalysis B: Environmental Volume: 印刷中 Pages: 1124-1129
  • DOI: 10.1016/j.apcatb.2018.01.046
  • NAID: 120006939381
  • Peer Reviewed
• [Journal Article] Functionalization of protein crystals with metal ions, complexes and nanoparticles (2018)
  • Author(s): Abe Satoshi、Maity Basudev、Ueno Takafumi
  • Journal Title: Current Opinion in Chemical Biology Volume: 43 Pages: 68-76
  • DOI: 10.1016/j.cbpa.2017.11.015
  • Peer Reviewed
• [Presentation] Functionalization of amyloid beta peptide into ferritin cage and observation of the cage disassembly. (2022)
  • Author(s): Basudev Maity, Shiori Kameyama, Satoshi Abe, Takafumi Ueno
  • Organizer: 102nd CSJ Annual Meeting
• [Presentation] Analysis of Dynamic Behavior of Aromatic Interactions using Protein Cages (2022)
  • Author(s): Yuki Hishikawa, Asuka Asanuma, Basudev Maity, Satoru Nagatoishi, Kouhei Tsumoto, Satoshi Abe, Takafumi Ueno
  • Organizer: 102nd CSJ Annual Meeting
• [Presentation] Dynamics structural analysis of miniprotein by in-cell crystal engineering (2022)
  • Author(s): Mariko Kojima, Yuki Hishikawa, Satoshi Abe, Tadaomi Furuta, Duy Phuoc Tran, Akio Kitao, Takafumi Ueno
  • Organizer: 102nd CSJ Annual Meeting
• [Presentation] 無細胞タンパク質結晶エンジニアリングによる構造解析 (2022)
  • Author(s): 田中 潤子, 安部 聡, 金丸 周司, 上野 隆史
  • Organizer: 102nd CSJ Annual Meeting
• [Presentation] In-cell crystal engineering for the development of solid biomaterials (2022)
  • Author(s): Satoshi Abe, Mariko Kojima, Junko Tanaka, Yuto Nakasuji, Takafumi Ueno
  • Organizer: 102nd CSJ Annual Meeting
• [Presentation] Structural investigation of a semi-clathrate hydrate formation using porous ferritin crystals (2022)
  • Author(s): Jiaxin Tian, Basudev Maity, Satoshi Abe, Takafumi Ueno
  • Organizer: 102nd CSJ Annual Meeting
• [Presentation] Design of the heteroepitaxial vertical assembly of asymmetric protein needles (2022)
  • Author(s): Kosuke Kikuchi, Takafumi Ueno
  • Organizer: 102nd CSJ Annual Meeting
• [Presentation] タンパク質ケージ内芳香族クラスターによる蛍光分子特性制御 (2021)
  • Author(s): 菱川湧輝, MAITYBASUDEV, 吉沢道人, 森本 正和, 安部聡, 上野隆史
  • Organizer: 第15回バイオ関連化学シンポジウム
• [Presentation] 迅速タンパク質結晶化とサブミクロン結晶構造解析 (2021)
  • Author(s): 安部 聡, 田中潤子, 上野隆史
  • Organizer: 第15回バイオ関連化学シンポジウム
• [Presentation] 無細胞タンパク質合成を利用した結晶化とサブミクロン結晶構造解析 (2021)
  • Author(s): 安部聡, 上野隆史
  • Organizer: 70th Symposium on Macromolecules
• [Presentation] Effect of Alanine substitution at the 2-fold symmetric interface of the ferritin cage (2021)
  • Author(s): Jiaxin Tian, Basudev Maity, Satoshi Abe, Kenji Yasuda, Takeshi Murata, Takafumi Ueno
  • Organizer: 70th Symposium on Macromolecules
• [Presentation] βヘリックスタンパク質分子針の末端設計による二次元集合制御 (2021)
  • Author(s): 菊池 幸祐, 福山達也, 古田 忠臣, 内橋 貴之, 前多裕介, 上野 隆史
  • Organizer: 第15回バイオ関連化学シンポジウム
• [Presentation] タンパク質分子針の末端構造設計による二次元集合パターン制御 (2021)
  • Author(s): 菊池 幸祐, 福山達也, 古田 忠臣, 前多裕介, 内橋 貴之, 上野 隆史
  • Organizer: 第１回発動分子科学研究会
• [Presentation] 細胞内タンパク質結晶を利用した天然変性タンパク質の構造解析 (2021)
  • Author(s): 小島摩利子, 安部聡, 上野隆史
  • Organizer: 第15回バイオ関連化学シンポジウム
• [Presentation] DESIGN OF IN-CELL PROTEIN CRYSTALS FOR DEVELOPMENT OF BIO-SOLID MATERIALS (2021)
  • Author(s): Satoshi Abe, Takafumi Ueno
  • Organizer: 22nd American Conference on Crystal Growth and Epitaxy (ACCGE-22)
  • Int'l Joint Research / Invited
• [Presentation] タンパク質ケージ結晶を用いた芳香族クラスターの動的挙動解析 (2021)
  • Author(s): 菱川湧輝, 野谷大樹, MAITYBASUDEV, 長門石 曉, 津本浩平, 安部聡, 上野隆史
  • Organizer: The 70th SPSJ Annual Meeting
• [Presentation] タンパク質分子針の末端設計による二次元集合パターン制御 (2021)
  • Author(s): 菊池幸祐, 福山達也, 古田忠臣, 前多裕介, 内橋貴之, 上野隆史
  • Organizer: The 70th SPSJ Annual Meeting
• [Presentation] The role of helix modification in building hybrid ferritin cages (2021)
  • Author(s): Jiaxin Tian, Kenji Yasuda, Basudev Maity, Satoshi Abe, Takeshi Murata, Takafumi Ueno
  • Organizer: The 70th SPSJ Annual Meeting
• [Presentation] β-ヘリックス人工分子針の動的集合設計 (2021)
  • Author(s): 菊池幸祐, 福山達也, Maity Basudev, 古田忠臣, 前多裕介, 内橋貴之, 上野隆史
  • Organizer: 日本化学会第101春季年会
• [Presentation] タンパク質ケージ内における芳香族分子集積と反応制御 (2021)
  • Author(s): 菱川湧輝, 野谷大樹, Basudev Maity, 安部聡, 上野隆史
  • Organizer: 日本化学会第101回春季年会
• [Presentation] Energy Analysis of Miniprotein by in Vivo Protein Crystallization (2021)
  • Author(s): 小島 摩利子, 菱川 湧輝, 安部 聡, 古田 忠臣, Tran Phuoc Duy, 北尾 彰朗, 上野 隆史
  • Organizer: 日本化学会第101春季年会
• [Presentation] Design of the two-dimensional protein assembly patterns by β-helical protein needle (2020)
  • Author(s): 菊池 幸祐, 上野 隆史
  • Organizer: 第69回高分子討論会
• [Presentation] 無細胞タンパク質合成による微小結晶構造解析 (2020)
  • Author(s): 小島摩利子, 安部聡, 上野隆史
  • Organizer: 第14回バイオ関連化学シンポジウム2020
• [Presentation] タンパク質ケージを利用した芳香族クラスターの構築と外来分子内包 (2020)
  • Author(s): 菱川湧輝, 野谷大樹, Basudev Maity, 安部聡, 上野隆史
  • Organizer: 発動分子科学第5回領域会議
• [Presentation] Construction of Fibril Protein Assemblies From Engineered Protein Crystals (2020)
  • Author(s): PHAM Toan Thuc, 安部 聡, 根岸 走, 上野 隆史
  • Organizer: 69th Symposium on Macromolecules
• [Presentation] Construction of Filament Protein Assembly From Autonomous Cross-Linked Crystals (2020)
  • Author(s): PHAM Toan Thuc, 安部 聡, 根岸 走, 上野 隆史
  • Organizer: 14th Bio-related Chemistry Symposium
• [Presentation] タンパク質結晶工学による新機能材料 (2020)
  • Author(s): 上野隆史
  • Organizer: 第28回ポリマー材料フォーラム
  • Invited
• [Presentation] GPCR 内包を目指したタンパク質ハイブリッド結晶の構築 (2020)
  • Author(s): 田中潤子, 安部聡, 長田俊哉, 上野隆史
  • Organizer: 第 10 回 CSJ 化学フェスタ
• [Presentation] Molecular design of protein needle for norovirus vaccine (2020)
  • Author(s): Nguyen Dan Que, 上野 隆史
  • Organizer: 第 10 回 CSJ 化学フェスタ
• [Presentation] Protein Crystals for Designing Multiple Nanofunctions (2020)
  • Author(s): 上野 隆史
  • Organizer: The 58th Annual Meeting of the Biophysical Society of Japan
  • Invited
• [Presentation] 細胞内タンパク質結晶化の最適化と迅速構造解析 (2020)
  • Author(s): 安部 聡, 小島摩利子, 小暮遼河, 上野隆史
  • Organizer: 第14回バイオ関連シンポジウム
• [Presentation] フェリチンケージのサブユニット界面を利用した多核金結合サイトの構築 (2020)
  • Author(s): 菱川湧輝, Basudev Maity, 安部聡, Diannan Lu, 上野隆史
  • Organizer: 第70回錯体化学会討論会
• [Presentation] タンパク質ケージの内部空間設計による芳香族クラスターの構築 (2020)
  • Author(s): 菱川湧輝, 野谷大樹, Basudev Maity, 安部聡, 上野隆史
  • Organizer: 第14回バイオ関連化学シンポジウム
• [Presentation] In vivo Protein Crystal Engineering for Structure Analysis of Meta-stable State Mini-protein (2020)
  • Author(s): 小島 摩利子, 安部 聡, 上野 隆史
  • Organizer: 第69回高分子討論会
• [Presentation] タンパク質の迅速結晶化とサブミクロン結晶の構造解析 (2020)
  • Author(s): 安部 聡, 小島摩利子, 上野隆史
  • Organizer: 第69回高分子討論会
• [Presentation] かご状タンパク質フェリチンのサブユニット界面における金集積機構の解明 (2020)
  • Author(s): 菱川湧輝, Basudev Maity, 安部聡, Diannan Lu, 上野隆史
  • Organizer: 第69回高分子学会年次大会
• [Presentation] タンパク質の細胞内迅速・微量結晶化と構造解析 (2020)
  • Author(s): 安部 聡, 小島摩利子, 小暮遼河, 上野隆史
  • Organizer: 第69回高分子学会年次大会
• [Presentation] Construction of Fibril Protein Assembly From Protein Crystals (2020)
  • Author(s): Thuc Toan PHAM,Satoshi Abe,Hashiru Negishi,Takafumi Ueno
  • Organizer: The 100th CSJ Annual Meeting
• [Presentation] ミニタンパク質融合多角体タンパク質の細胞内結晶化 (2020)
  • Author(s): 小島摩利子,安部聡,上野隆史
  • Organizer: 日本化学会第100春季年会
• [Presentation] Design of the Two-dimensional Protein Assembly Patterns by Modifying the Tip of β-helical Protein Needle (2020)
  • Author(s): Kosuke Kikuchi,Takafumi Ueno
  • Organizer: The 100th CSJ Annual Meeting
• [Presentation] タンパク質の迅速構造解析を目指した多角体の結晶化設計 (2020)
  • Author(s): 安部 聡,小島摩利子,小暮遼河,上野隆史
  • Organizer: 日本化学会 第100春季年会（2020）
• [Presentation] 細胞内結晶化の最適化 (2020)
  • Author(s): 小暮遼河,安部 聡,上野隆史
  • Organizer: 日本化学会 第100春季年会（2020）
• [Presentation] Disassembly reaction of the ferritin cage observed by high-speed AFM (2020)
  • Author(s): MAITY Basudev,Zhipeng LI,Kento NIWASE,Diannan LU,Takafumi UENO,Takafumi
  • Organizer: The 100th Annual Meeting 2020 of CSJ
• [Presentation] Artifical enzymes Artifical enzymes using protein assemblies (2020)
  • Author(s): Takafumi Ueno
  • Organizer: 8th ELSI SYMPOSIUM EXTENDING VIEWS OF CATALYSIS
  • Int'l Joint Research / Invited
• [Presentation] 外来タンパク質固定化のための細胞内タンパク質結晶の空間設計 (2019)
  • Author(s): 安部 聡,上野隆史
  • Organizer: 日本結晶学会令和元年（2019年度）年会
• [Presentation] 高速原子間力顕微鏡による針状タンパク質の集合体構造の解析 (2019)
  • Author(s): 菊池幸祐,上野隆史
  • Organizer: 第9回CSJ化学フェスタ2019
• [Presentation] Engineering of Protein Assembly? for Coordination Chemistry (2019)
  • Author(s): Takafumi Ueno
  • Organizer: 12th CJJSMCC 2019
  • Int'l Joint Research / Invited
• [Presentation] アミロイドβ融合蛋白質ケージの設計とその動的挙動解析 (2019)
  • Author(s): 亀山志織,MAITYBASUDEV,安部聡,上野隆史
  • Organizer: 第9回CSJ化学フェスタ
• [Presentation] 多角体タンパク質の結晶化設計 (2019)
  • Author(s): 安部 聡,小島摩利子,上野隆史
  • Organizer: 第13回バイオ関連化学シンポジウム
• [Presentation] 原子、分子そして生命へ (2019)
  • Author(s): 上野隆史
  • Organizer: 錯体化学会第69回討論会
  • Invited
• [Presentation] ミニタンパク質融合多角体タンパク質の結晶設計 (2019)
  • Author(s): 小島摩利子,安部聡,上野隆史
  • Organizer: 第13回バイオ関連化学シンポジウム
• [Presentation] 高速原子間力顕微鏡による針状タンパク質の集合体観察 (2019)
  • Author(s): 菊池幸祐,上野隆史
  • Organizer: 第68回高分子討論会
• [Presentation] アミロイドβオリゴマー形成機序の解明に向けた融合蛋白質ケージの設計 (2019)
  • Author(s): 亀山志織,MAITYBASUDEV,安部聡,上野隆史
  • Organizer: 第13回バイオ関連化学シンポジウム
  • Int'l Joint Research
• [Presentation] 球状タンパク質フェリチンを用いたアミロイドβオリゴマーの動的挙動追跡 (2019)
  • Author(s): 亀山志織,MAITYBASUDEV,安部聡,上野隆史
  • Organizer: 第68回高分子討論会
• [Presentation] Construction of Supramolecular Structures from Protein Crystals (2019)
  • Author(s): Takafumi Ueno
  • Organizer: 10th International Conference on Materials for Advanced Technologies
  • Int'l Joint Research / Invited
• [Presentation] Construction of Protein Needle with Cargo Protein from Norovirus (2019)
  • Author(s): Que Dan NGUYEN,Kazuhiko Katayama,Takafumi Ueno
  • Organizer: 10th International Conference on Materials for Advanced Technologies
  • Int'l Joint Research
• [Presentation] Assembly Formation of Needle-Like Protein from Bacteriophage T4 Observed by High Speed Atomic Force Microscopy (2019)
  • Author(s): Kosuke Kikuchi,Kento Niwase,Takafumi Ueno
  • Organizer: 10th International Conference on Materials for Advanced Technologies
  • Int'l Joint Research
• [Presentation] In Cell Protein Crystals Containing Organometallic Complexes (2019)
  • Author(s): Satoshi Abe,Takafumi Ueno
  • Organizer: 15th International Symposium on Applied Bioinorganic Chemistry
  • Int'l Joint Research
• [Presentation] Creation of functional materials using in vivo protein crystals (2019)
  • Author(s): Satoshi Abe,Makoto Kasamatsu,Takafumi Ueno
  • Organizer: 第68回高分子年次大会
• [Presentation] In vivo crystallization of polyhedra protein fused with metal binding peptide” (2019)
  • Author(s): Mariko Kojima,Satoshi Abe,Takafumi Ueno
  • Organizer: 第68回高分子学会年次大会
• [Presentation] Protein Crystal Engineering for Coordination Chemistry (2019)
  • Author(s): Takafumi Ueno
  • Organizer: China-Japan Symposium of Functional Coordination Chemistry
  • Int'l Joint Research / Invited
• [Presentation] In cell design of protein assembly materials (2019)
  • Author(s): Takafumi Ueno
  • Organizer: Japan-BIOCEV conference
  • Int'l Joint Research / Invited
• [Presentation] Solid-biocatalysts constructed from immobilized enzymes within in vivo protein crystals (2019)
  • Author(s): Tien Khanh Nguyen,Satoshi Abe,Takafumi Ueno
  • Organizer: 13th Biofunctioned conference
• [Presentation] Solid biocatalysts designed from in vivo protein crystals (2019)
  • Author(s): Tien Khanh Nguyen,Satoshi Abe,Takafumi Ueno
  • Organizer: 68th Symposium on Macromolecules
• [Presentation] タンパク質結晶工学による新機能材料 (2019)
  • Author(s): 上野隆史
  • Organizer: 第28回ポリマー材料フォーラム
  • Invited
• [Presentation] Protein crystals for designing biosupramolecular materials (2019)
  • Author(s): Takafumi Ueno
  • Organizer: Tokyo Tech NCCR Molecular Systems Engineering Joint Symposium, Basel,
  • Int'l Joint Research / Invited
• [Presentation] Bioinorganic Design of Protein Assembly (2019)
  • Author(s): Takafumi Ueno
  • Organizer: 2019 KAIST School of Molecular Science BK21 Workshop
  • Int'l Joint Research / Invited
• [Presentation] Metal Coordination Functions Designed in Protein Cage (2018)
  • Author(s): Takafumi Ueno
  • Organizer: 3rd Japan-UK Joint Symposium on Coordination Chemistry
  • Int'l Joint Research / Invited
• [Presentation] Metal Coordination and Function Designed in Protein Assembly (2018)
  • Author(s): Takafumi Ueno
  • Organizer: 2018 Korea-Taiwan-Japan Bioinorganic Chemistry Symposium
  • Int'l Joint Research / Invited
• [Presentation] Functional Design of Protein Cage for Sustainable Bionanomaterial (2018)
  • Author(s): Takafumi Ueno
  • Organizer: ASPIRE League forum
  • Int'l Joint Research / Invited
• [Presentation] Functions designed in Crystalline Protein Assembly? (2018)
  • Author(s): Takafumi Ueno
  • Organizer: JST Fujita ACCEL International Symposium Coordination Chemistry for Structural Elucidation ICCC2018?
  • Int'l Joint Research / Invited
• [Presentation] Dynamic Process of Proteins Observed in Crystalline Cages (2018)
  • Author(s): Takafumi Ueno
  • Organizer: The 79th Okazaki Conference Synthetic, Biological, and Hybrid Molecular Engines
  • Int'l Joint Research / Invited
• [Presentation] Dynamic Function of Crystalline Protein Assembly (2018)
  • Author(s): Takafumi Ueno
  • Organizer: ACSIN-14 & ICSPM26, Nanobiology
  • Int'l Joint Research / Invited
• [Presentation] Artificial metalloproteins constructed with protein assemblies for bioinorganic materials (2018)
  • Author(s): Takafumi Ueno
  • Organizer: The 18th Japan-Korea Joint Symposium on Organometallic and Coordination Chemistry
  • Int'l Joint Research / Invited
• [Presentation] Artificial metalloproteins constructed with protein assemblies for bionano applications (2018)
  • Author(s): Takafumi Ueno
  • Organizer: The 9th Asian Biological Inorganic Conference
  • Int'l Joint Research / Invited
• [Book] Cell-Inspired Materials and Engineering (2021)
  • Author(s): H. Tabe and T. Ueno
  • Total Pages: 257
  • Publisher: Springer
• [Book] 機能性高分子金属錯体 (2020)
  • Author(s): 安部 聡、上野隆史
  • Publisher: 三共出版
• [Book] 光エネルギー変換における分子触媒の新展開 (2020)
  • Author(s): 安部 聡、上野隆史
  • Publisher: 化学同人
• [Remarks] Ueno Lab
  • URL: http://www.ueno.bio.titech.ac.jp/
• [Remarks] ueno lab
  • URL: http://www.ueno.bio.titech.ac.jp/index.html
• [Patent(Industrial Property Rights)] ＭＨＣ分子に適合する病原微生物由来のペプチドが担持された複合ポリペプチド単量体、当該単量体の会合体、及び当該会合体を有効成分とするコンポーネントワクチン (2021)
  • Inventor(s): 上野 隆史／Nguyen Dan Que
  • Industrial Property Rights Holder: 上野 隆史／Nguyen Dan Que
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2021-001522
  • Filing Date: 2021
• [Patent(Industrial Property Rights)] コロナウイルスの蛋白質が担持された複合ポリペプチド単量体、当該単量体の会合体、及び当該会合体を有効成分とするコンポーネントワクチン (2020)
  • Inventor(s): 上野隆史、片山和彦
  • Industrial Property Rights Holder: 上野隆史、片山和彦
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2020-088508
  • Filing Date: 2020
• [Patent(Industrial Property Rights)] 融合タンパク質及びその使用 (2019)
  • Inventor(s): 上野隆史、亀山志織
  • Industrial Property Rights Holder: 上野隆史、亀山志織
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-088420
  • Filing Date: 2019