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Development of single-molecule methods measuring energy conversion processes of biological and synthetic molecular engines

Planned Research

Project AreaMolecular Engine: Design of Autonomous Functions through Energy Conversion
Project/Area Number 18H05424
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Complex systems
Research InstitutionInstitute for Molecular Science

Principal Investigator

IINO RYOTA  分子科学研究所, 生命・錯体分子科学研究領域, 教授 (70403003)

Co-Investigator(Kenkyū-buntansha) 笠井 倫志  岐阜大学, 糖鎖生命コア研究所, 特任准教授 (20447949)
Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥125,190,000 (Direct Cost: ¥96,300,000、Indirect Cost: ¥28,890,000)
Fiscal Year 2022: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2021: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2020: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2019: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2018: ¥33,020,000 (Direct Cost: ¥25,400,000、Indirect Cost: ¥7,620,000)
Keywords分子モーター / 1分子計測 / 分子機械 / 1分子イメージング / 光学顕微鏡
Outline of Final Research Achievements

We developed new single-molecule measurement method to visualize the movement and energy conversion process of biological, artificial, and hybrid molecular engines that convert energy while moving autonomously. Major achievements include: 1) development of a method to track the movement of biological and hybrid molecular engines with atomic-level localization precision, 2) development of a method to simultaneously track the movement of multiple types of molecular engines with high time resolution and high localization precision, 3) development of a method to track the movement of artificial molecular engines in living cells, and 4) elucidation of the operational mechanisms of molecular engines using the developed method.

Academic Significance and Societal Importance of the Research Achievements

発動分子が自律的に動き続けながら高効率にエネルギーを変換する仕組みを理解するには、エネルギー変換を行う際の発動分子の動きを原子レベルで詳細に理解することが重要である。本研究で開発した1分子計測法を様々な発動分子へと適用することで、その仕組みの理解がさらに深まると期待される。そして将来的には、新たな高効率エネルギー変換デバイス(例えば発電素子)の開発、原理実証、および社会実装につながると期待される。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (99 results)

All 2023 2022 2021 2020 2019 2018 Other

All Journal Article (25 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 21 results,  Open Access: 15 results) Presentation (65 results) (of which Int'l Joint Research: 24 results,  Invited: 44 results) Remarks (6 results) Patent(Industrial Property Rights) (2 results) Funded Workshop (1 results)

  • [Journal Article] Direct observation of stepping rotation of V-ATPase reveals rigid component in coupling between Vo and V1 motors2022

    • Author(s)
      Otomo Akihiro、Iida Tatsuya、Okuni Yasuko、Ueno Hiroshi、Murata Takeshi、Iino Ryota
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 42

    • DOI

      10.1073/pnas.2210204119

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A methodology for creating mutants of G‐protein coupled receptors stabilized in active state by combining statistical thermodynamics and evolutionary molecular engineering2022

    • Author(s)
      Yamamoto Taisei、Yasuda Satoshi、Kasai Rinshi S.、Nakano Ryosuke、Hikiri Simon、Sugaya Kanna、Hayashi Tomohiko、Ogasawara Satoshi、Shiroishi Mitsunori、Fujiwara Takahiro K.、Kinoshita Masahiro、Murata Takeshi
    • Journal Title

      Protein Science

      Volume: 31 Issue: 10

    • DOI

      10.1002/pro.4425

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover2022

    • Author(s)
      Burton-Smith RN, Song C, Ueno H, Murata T, Iino R, Murata K
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2022.08.09.503272

    • Related Report
      2022 Annual Research Report
    • Open Access
  • [Journal Article] はたらく分子マシン:ナノの世界のエネルギー変換へ2022

    • Author(s)
      金原数, 飯野亮太, 竹内正之, 前多裕介
    • Journal Title

      現代化学

      Volume: 612 Pages: 46-50

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Positive Charge Introduction on the Surface of Thermostabilized PET Hydrolase Facilitates PET Binding and Degradation2021

    • Author(s)
      Nakamura Akihiko、Kobayashi Naoya、Koga Nobuyasu、Iino Ryota
    • Journal Title

      ACS Catalysis

      Volume: 11 Issue: 14 Pages: 8550-8564

    • DOI

      10.1021/acscatal.1c01204

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional Reconstitution of Dopamine D2 Receptor into a Supported Model Membrane in a Nanometric Confinement2021

    • Author(s)
      Nagai Rurika、Sugimachi Ayane、Tanimoto Yasushi、Suzuki Kenichi G. N.、Hayashi Fumio、Weikert Dorothee、Gmeiner Peter、Kasai Rinshi S.、Morigaki Kenichi
    • Journal Title

      Advanced Biology

      Volume: 5 Issue: 12 Pages: 2100636-2100636

    • DOI

      10.1002/adbi.202100636

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] はたらく分子マシン10:生体分子モーターの予想外の動きを観る2021

    • Author(s)
      飯野亮太
    • Journal Title

      現代化学

      Volume: 604 Pages: 19-23

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 生きものが機械でもいいじゃない2021

    • Author(s)
      飯野亮太
    • Journal Title

      Seibutsu Butsuri

      Volume: 61 Issue: 2 Pages: 077-077

    • DOI

      10.2142/biophys.61.077

    • NAID

      130008002752

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Journal Article] 銀, 金, 銀金合金ナノ粒子の光散乱を利用したマルチカラー生体1分子追跡2021

    • Author(s)
      安藤潤,飯野亮太
    • Journal Title

      フォトニクスニュース

      Volume: 6 Pages: 132-136

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combined approach to engineer a highly active mutant of processive chitinase hydrolyzing crystalline chitin2020

    • Author(s)
      Visootsat A, Nakamura A, Wang T-W, Iino R
    • Journal Title

      ACS Omega

      Volume: 5 Issue: 41 Pages: 26807-26816

    • DOI

      10.1021/acsomega.0c03911

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases2020

    • Author(s)
      Nakamura A, Ishiwata D, Visootsat A, Uchiyama T, Mizutani K, Kaneko S, Murata T, Igarashi K, Iino R
    • Journal Title

      J Biol Chem

      Volume: 295 Issue: 43 Pages: 14606-14617

    • DOI

      10.1074/jbc.ra120.014792

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-speed single-molecule imaging reveals signal transduction by onduced transbilayer raft phases.2020

    • Author(s)
      I. Koyama-Honda, T. K. Fujiwara, R. S. Kasai, K. G. N. Suzuki, E. Kajikawa, T. A. Tsunoyama, A. Kusumi.
    • Journal Title

      J. Cell Biol.

      Volume: 219 Issue: 12

    • DOI

      10.1083/jcb.202006125

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synergetic roles of Formyl Peptide Receptor 1 oligomerization in ligand-induced signal transduction2020

    • Author(s)
      Nishiguchi T, Yoshimura H, Kasai RS, Fujiwara TK, Ozawa T
    • Journal Title

      ACS Chem. Biol.

      Volume: 15 Issue: 9 Pages: 2577-2587

    • DOI

      10.1021/acschembio.0c00631

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A synthetic ion channel with anisotropic ligand response2020

    • Author(s)
      Muraoka Takahiro, Noguchi Daiki, Kasai Rinshi, Sato Kohei, Sasaki Ryo, Tabata Kazuhito, Ekimoto Toru, Ikeguchi Mitsunori, Kamagata Kiyoto, Hoshino Norihisa, Noji Hiroyuki, Akutagawa Tomoyuki, Ichimura Kazuaki, Kinbara Kazushi
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 2924-2924

    • DOI

      10.1038/s41467-020-16770-z

    • NAID

      120006878529

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Small stepping motion of processive dynein revealed by load-free high-speed single-particle tracking2020

    • Author(s)
      Ando J, Shima T, Kanazawa R, Shimo-Kon R, Nakamura A, Yamamoto M, Kon T, Iino R
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 1080-1080

    • DOI

      10.1038/s41598-020-58070-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens2020

    • Author(s)
      Visootsat A, Nakamura A, Vignon P, Watanabe H, Uchihashi T, Iino R
    • Journal Title

      J Biol Chem

      Volume: 295 Issue: 7 Pages: 1915-1925

    • DOI

      10.1074/jbc.ra119.012078

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Introduction: Molecular Motors2020

    • Author(s)
      Iino Ryota、Kinbara Kazushi、Bryant Zev
    • Journal Title

      Chemical Reviews

      Volume: 120 Issue: 1 Pages: 1-4

    • DOI

      10.1021/acs.chemrev.9b00819

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Multicolor high-speed tracking of single biomolecules with silver, gold, silver-gold alloy nanoparticles2019

    • Author(s)
      Ando J, Nakamura A, Yamamoto M, Song C, Murata K, Iino R
    • Journal Title

      ACS Photonics

      Volume: 6 Issue: 11 Pages: 2870-2883

    • DOI

      10.1021/acsphotonics.9b00953

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Defining raft domains in the plasma membrane2019

    • Author(s)
      Akihiro Kusumi、Takahiro K. Fujiwara、Taka A. Tsunoyama、Rinshi S. Kasai、An‐An Liu、Koichiro M. Hirosawa、Masanao Kinoshita、Nobuaki Matsumori、Naoko Komura、Hiromune Ando、Kenichi G. N. Suzuki
    • Journal Title

      Traffic

      Volume: 21 Issue: 1 Pages: 106-137

    • DOI

      10.1111/tra.12718

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies2019

    • Author(s)
      Takayuki Umakoshi, Shingo Fukuda, Ryota Iino, Takayuki Uchihashi, Toshio Ando*
    • Journal Title

      BBA General Subjects

      Volume: 印刷中 Issue: 2 Pages: 129325-129325

    • DOI

      10.1016/j.bbagen.2019.03.011

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous observation of kinesin-driven microtubule motility and binding of adenosine triphosphate using linear zero-mode waveguides2018

    • Author(s)
      Fujimoto K, Morita Y, Iino R, Tomishige M, Shintaku H, Kotera H, Yokokawa R
    • Journal Title

      ACS Nano

      Volume: 12 Issue: 12 Pages: 11975-11985

    • DOI

      10.1021/acsnano.8b03803

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy2018

    • Author(s)
      Tsunoda Jun、Song Chihong、Imai Fabiana Lica、Takagi Junichi、Ueno Hiroshi、Murata Takeshi、Iino Ryota、Murata Kazuyoshi
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 15632-15632

    • DOI

      10.1038/s41598-018-33977-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin2018

    • Author(s)
      Nakamura Akihiko、Okazaki Kei-ichi、Furuta Tadaomi、Sakurai Minoru、Iino Ryota
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 3814-3814

    • DOI

      10.1038/s41467-018-06362-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visualization of Functional Structure and Kinetic Dynamics of Cellulases2018

    • Author(s)
      Akihiko Nakamura, Ryota Iino
    • Journal Title

      Adv Exp Med Biol

      Volume: 1104 Pages: 201-217

    • DOI

      10.1007/978-981-13-2158-0_10

    • ISBN
      9789811321573, 9789811321580
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Class-A GPCR Dopamine D2 Receptor Forms Transient Dimers Stabilized by Agonists: Detection by Single-Molecule Tracking2018

    • Author(s)
      Kasai Rinshi S.、Ito Shuichi V.、Awane Ryo M.、Fujiwara Takahiro K.、Kusumi Akihiro
    • Journal Title

      Cell Biochemistry and Biophysics

      Volume: 76 Issue: 1-2 Pages: 29-37

    • DOI

      10.1007/s12013-017-0829-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 生体・人工ハイブリッド分子モーターの創出と特性解析2023

    • Author(s)
      飯野亮太
    • Organizer
      分子研研究会 「生体分子材料を探る:発動分子のさらなる理解と設計に向けて」
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Engineering rotary and linear molecular motor proteins2023

    • Author(s)
      Ryota Iino
    • Organizer
      The 3rd NINS-Princeton Joint Symposium
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞外小胞を介したWntのシグナル伝達機構の解明2023

    • Author(s)
      笠井 倫志、鈴木 健一
    • Organizer
      2022年度生物物理学会中部支部講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 2色同時超解像動画観察によるGPI-アンカー型タンパク質の膜ドメイン形成機構の解明2023

    • Author(s)
      川合 登偉、笠井 倫志、廣澤 幸一朗、横田 康成、鈴木 健一
    • Organizer
      2022年度生物物理学会中部支部講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 細胞膜内層脂質ドメインによるシグナル伝達場形成の検証2023

    • Author(s)
      森 俊貴、廣澤 幸一朗、笠井 倫志、田口 友彦、横田 康成、鈴木 健一
    • Organizer
      2022年度生物物理学会中部支部講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 高速AFMと局在化AFM法を用いた接着GPCRの細胞外領域の構造解析2023

    • Author(s)
      西口 茂孝、笠井 倫志、内橋 貴之
    • Organizer
      2022年度生物物理学会中部支部講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Single-molecule analysis and engineering of motor proteins2022

    • Author(s)
      Ryota Iino
    • Organizer
      iNANO-IMS-ExCELLS Interdisciplinary Nanoscience Joint Meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 分子モーターの動きをみる、動きをつくる2022

    • Author(s)
      飯野亮太
    • Organizer
      第36回分子シミュレーション討論会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 分子モーターの動きをみる、動きをつくる2022

    • Author(s)
      飯野亮太
    • Organizer
      名古屋大学理学研究科談話会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 結晶性高分子分解酵素の反応サイクルの1分子イメージング解析2022

    • Author(s)
      飯野亮太
    • Organizer
      第19回糖鎖科学コンソーシアムシンポジウム
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Engineering linear motor protein kinesin-12022

    • Author(s)
      Ryota Iino
    • Organizer
      EASMB 2022 Symposium
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-molecule analysis and engineering of Na+-pumping V-ATPase2022

    • Author(s)
      Akihiro Otomo, Ryota Iino
    • Organizer
      EASMB 2022 Symposium
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Integrated research on rotary V-ATPase approached by single-molecule observation, biochemical assay, and protein engineering2022

    • Author(s)
      Akihiro Otomo, Ryota Iino
    • Organizer
      The 60th Annual Meeting of the Biophysical Society of Japan "Tackle “Molecular Engine” by Early-career Researchers"
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Single-molecule analysis and engineering of molecular motor proteins2022

    • Author(s)
      Ryota Iino
    • Organizer
      Sendai 2022, An Update on Molecular Machines: Open Challenges and New Perspectives
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] リニア分子モーターキネシン1のエンジニアリング2022

    • Author(s)
      飯野亮太
    • Organizer
      第22回日本蛋白質科学会年会ワークショップ「発動分子エンジニアリング:タンパク質分子機械をいじり倒して実現する新機能」
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Trans-dimer conformations of full-length ectodomains of Celsr cadherin in solution visualized using high-speed atomic force microscopy2022

    • Author(s)
      Nishiguchi S, Kasai R, Uchihashi T
    • Organizer
      The 60th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2022 Annual Research Report
  • [Presentation] Single molecule observation of polarity-related membrane proteins at the cell-cell interface; immobilization and accumulation of Frizzled2022

    • Author(s)
      Kasai RS, Nemoto YL
    • Organizer
      The 60th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2022 Annual Research Report
  • [Presentation] Reconstitution of exosomes into a patterned model membrane2022

    • Author(s)
      Yoshimura Y, Sugimachi A, Hayashi F, Hirosawa KM, Kasai RS, Suzuki KGN, Morigaki K
    • Organizer
      The 60th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2022 Annual Research Report
  • [Presentation] レーザー暗視野顕微鏡を用いた高速高精度生体1分子イメージング2022

    • Author(s)
      飯野亮太
    • Organizer
      レーザー学会学術講演会第42回年次大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] マクロな機械とナノサイズの分子機械に共通な仕組みはあるか2022

    • Author(s)
      飯野亮太
    • Organizer
      ソフトロボット×発動分子科学ジョイントシンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 話題提供:生命科学の立場から2022

    • Author(s)
      飯野亮太
    • Organizer
      公開シンポジウム:アト秒レーザー科学研究施設(ALFA)計画の現状と展望
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 生体分子モーターを観る、壊す、創る2021

    • Author(s)
      飯野亮太
    • Organizer
      新学術「生命金属科学」領域会議
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Invited
  • [Presentation] Visualization and Engineering of the Motions of Protein Molecular Motors2021

    • Author(s)
      Ryota Iino
    • Organizer
      Seminar at Mechanobiology Institute, National University of Singapore
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Watching dynamic motions of natural and engineered molecular motor proteins2021

    • Author(s)
      Ryota Iino
    • Organizer
      Seminar on single-molecule biophysics, the School of Pharmacy, Huazhong University of Science and Technology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質分子モーターを観る、壊す、創る2021

    • Author(s)
      飯野亮太
    • Organizer
      第5回分子ロボティクス年次大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Engineering of hybrid kinesin-1 dimer with synthetic linker by tuning the neck linker length2021

    • Author(s)
      Jakia Jannat Keya, Akasit Visootsat, Akihiro Otomo, Sanghun Han, Kazushi Kinbara, Ryota Iino
    • Organizer
      Japan-US symposium on cytoskeletal motor proteins and their associated proteins, the 59th annual meeting of the Biophysical Society of Japan
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Engineering linear molecular motor kinesin-12021

    • Author(s)
      Ryota Iino
    • Organizer
      3rd Conference on Biomotors, Virus Assembly, and Nanobiotechnology Applications
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Watching dynamic motions of individual protein motors2021

    • Author(s)
      Ryota Iino
    • Organizer
      Pacifichem 2021, "Molecular engines based on energy conversion: From design to autonomous functions"
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional reconstitution of dopamine D2 receptor into a supported model membrane in a nanometric confinement2021

    • Author(s)
      Nagai R, Sugimachi A, Tanimoto Y, Suzuki KGN, Hayashi F, Kasai RS, Morigaki K
    • Organizer
      ACS Spring 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Single molecule observation of polarity-related membrane proteins at the cell-cell interface2021

    • Author(s)
      Kasai RS, Nemoto YL
    • Organizer
      The 59th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2021 Annual Research Report
  • [Presentation] Watching unexpected motions of protein molecular motors2020

    • Author(s)
      Ryota Iino
    • Organizer
      Serendipity Symposium 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生体分子モーターを観る、壊す、創る2020

    • Author(s)
      飯野亮太
    • Organizer
      東北大学大学院工学研究科応用物理学セミナー
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] タンパク質分子モーターを観る、壊す、創る2020

    • Author(s)
      飯野亮太
    • Organizer
      東京大学大学院工学系研究科化学生命工学専攻「ハイブリッドレクチャー」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] タンパク質の基礎2020

    • Author(s)
      飯野亮太
    • Organizer
      新学術領域合同シンポジウム-ソフトロボット学と発動分子科学の境界-
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] High-speed atomic force microscopy as a versatile tool to study dynamical and mechanical properties of proteins2020

    • Author(s)
      Christian Ganser, Kimitoshi Takeda, Ryota Iino, Koichi Kato, Takayuki Uchihashi
    • Organizer
      The 58th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] Single-molecule analysis of rotation of Enterococcus hirae V-ATPase depending on sodium ion concentration2020

    • Author(s)
      Akihiro Otomo, Tatsuya Iida, Hiroshi Ueno, Takeshi Murata, Ryota Iino
    • Organizer
      The 58th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] Combination approach for identification of highly-active mutant of processive chitinase2020

    • Author(s)
      Akasit VISOOTSAT, Akihiko NAKAMURA, Tak-Wai WANG, Ryota IINO
    • Organizer
      The 58th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] Single-molecule analysis of artificial kinesin-1 dimers and trimers with different linker lengths2020

    • Author(s)
      Kimitoshi Takeda, Monique Honsa, Akihiko Nakamura, Jun Ando, Ryota Iino
    • Organizer
      The 58th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] 蛍光1分子観察法による細胞膜中の分子の動態と機能の解明:会合体形成とダイナミクス2020

    • Author(s)
      笠井倫志
    • Organizer
      日本化学会秋季事業 第10会 CSJ化学フェスタ2020
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Single molecule observation of adhesion GPCR accumulated at the cell-cell interface2020

    • Author(s)
      Kasai RS, Nemoto YL.
    • Organizer
      The 58th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] Visualizing Dynamic Motions of Protein Molecular Motors with Plasmonic Nanoprobes2020

    • Author(s)
      Ryota Iino
    • Organizer
      The 1st International Symposium on Molecular Engine
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Processive chitinase: a burnt-bridge Brownian motor hydrolyzing crystalline polysaccharide2020

    • Author(s)
      Ryota Iino
    • Organizer
      The symposium "Molecular Motors" at the Biophysical Society 64th Annual Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plasmonic nanoprobes visualizing dynamic motions of single motor proteins2019

    • Author(s)
      Ryota Iino
    • Organizer
      Joint Meeting for Young Researchers "Frontiers in Imaging Probes and Technologies"
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Watching motor protein dynamics with plasmonic nanoprobes2019

    • Author(s)
      Ryota Iino
    • Organizer
      2nd East Asian Symposium on Single-Molecule Biological Sciences
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualizing dynamic motions of protein molecular motors with plasmonic nanoprobes2019

    • Author(s)
      Ryota Iino
    • Organizer
      2019 Japan-Korea Molecular Science Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Watching dynamic motions of hydrolysis-powered protein motors with plasmonic nanoprobes2019

    • Author(s)
      Ryota Iino
    • Organizer
      The 10th Toyota RIKEN internatonal workshop
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-molecule dynamics of motor proteins visualized by plasmonic nanoprobes2019

    • Author(s)
      Ryota Iino
    • Organizer
      2nd NIBB-Princeton Joint Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] プラズモニックナノプローブでタンパク質分子モーターの動きを観る2019

    • Author(s)
      飯野亮太
    • Organizer
      OCU先端光科学シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] プラズモニックナノ粒子を用いた分子モーターの高速高精度1分子イメージング2019

    • Author(s)
      飯野亮太
    • Organizer
      第9回分子モーター討論会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] バイオマス多糖を効率的に分解する生体分子モーターの作動原理2019

    • Author(s)
      飯野亮太
    • Organizer
      高分子学会バイオミメティクス研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Single-molecule dynamics of protein molecular motors visualized by plasmonic nanoprobes2019

    • Author(s)
      Ryota Iino
    • Organizer
      The workshop in NCCR
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 1分子計測、構造解析、シミュレーションで探るタンパク質分子モーターの作動機構2019

    • Author(s)
      飯野亮太
    • Organizer
      第24回べん毛研究交流会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Molecular engines: An introduction2018

    • Author(s)
      Ryota Iino
    • Organizer
      The 79th Okazaki Conference: Synthetic, Biological, and Hybrid Molecular Engines
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-molecular videography of protein molecular motors2018

    • Author(s)
      Ryota Iino
    • Organizer
      Telluride Workshop on "Molecular Videography"
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Commonalities and disparities in mechanisms of protein molecular motors revealed by single-molecule imaging analyses with plasmonic nanoprobes2018

    • Author(s)
      Ryota Iino
    • Organizer
      1st SNU Evolutionary Materials Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] バイオマス多糖を効率的に分解する生体分子モーターの作動原理2018

    • Author(s)
      飯野亮太
    • Organizer
      第67回高分子討論会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Characterization and engineering of chitin-hydrolyzing Brownian linear motor from marine bacteria2018

    • Author(s)
      Akihiko Nakamura, Veda Boorla, Hiroki Watanabe, Takayuki Uchihashi, Ryota Iino
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Multi-color single-molecule imaging with silver, gold, and silver/gold-alloy nanoparticles2018

    • Author(s)
      Jun Ando, Akihiko Nakamura, Mayuko Yamamoto, Ryota Iino
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Single-particle tracking of motor domain of a processive dynein at microsecond time resolution and nanometer localization precision2018

    • Author(s)
      Jun Ando, Tomohiro Shima, Akihiko Nakamura, Akasit Visootsat, Mayuko Yamamoto, Takahide Kon, Ryota Iino
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Estimating a diffusion model hopping on multiple energy surfaces from trajectories: Toward application to biomolecular motors2018

    • Author(s)
      Kei-ichi Okazaki, Akihiko Nakamura, Ryota Iino
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Single Particle Analysis of EhV-ATPase by Phase-Plate electron cryo-microscopy2018

    • Author(s)
      Jun Tsunoda, Chihong Song, Yakushiji Fabiana Lica, Takeshi Murata, Hiroshi Ueno, Naoyuki Miyazaki, Kenji Iwasaki, Junichi Takagi, Ryota Iino, Kazuyoshi Murata
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Transient dimer formation of G-protein coupled receptor: single fluorescent molecule imaging in live cells2018

    • Author(s)
      Kasai RS
    • Organizer
      The 3rd Biosignal Research Center International Symposium “Modulation of GPCR signaling by membrane heterogeneity and molecular clustering”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Gタンパク質共役型受容体の動的なダイマー形成:蛍光1 分子観察法による解明2018

    • Author(s)
      笠井倫志
    • Organizer
      日本農芸化学会 中部支部 第184回例会 若手シンポジウム “化学と生物のマリアージュ:若手研究者による生化学研究の新機軸”
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Spontaneous activation in a transient GPCR dimer before ligation as revealed by dual-channel single fluorescent molecule imaging2018

    • Author(s)
      Kasai RS
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Transient dimers of GPCRs are responsible for triggering GPCRs’ basic constitutive signals - A finding by the two-color single fluorescent-molecule tracking in living cells2018

    • Author(s)
      Kasai RS, Fujiwara TK, Kusumi A
    • Organizer
      Cold Spring Harbor meeting: Single Biomolecules
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 自然科学研究機構 分子科学研究所 飯野グループ

    • URL

      https://groups.ims.ac.jp/organization/iino_g/index.html

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report 2018 Annual Research Report
  • [Remarks] 分子研飯野グループホームページ

    • URL

      https://groups.ims.ac.jp/organization/iino_g/index.html

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] 二本足のリニア分子モーターダイニンは小さな歩幅でふらふら歩く(飯野グループら)

    • URL

      https://www.ims.ac.jp/news/2020/01/24_4544.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 光学顕微鏡によるマルチカラー高速高精度1分子観察を実現(飯野グループら)

    • URL

      https://www.ims.ac.jp/news/2019/11/25_4477.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] キチン加水分解酵素は熱ゆらぎを利用して一方向に動きながら結晶性バイオマスを分解する分子モノレールカー

    • URL

      https://www.ims.ac.jp/news/2018/09/19_4057.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] モータータンパク質とATPの結合を1分子で可視化することに成功

    • URL

      https://www.ims.ac.jp/news/2018/12/03_4138.html

    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] エクソソーム膜結合分子の解析方法2022

    • Inventor(s)
      森垣 憲一、鈴木 健一、笠井 倫志、廣澤 幸一朗
    • Industrial Property Rights Holder
      神戸大学、岐阜大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-132291
    • Filing Date
      2022
    • Related Report
      2022 Annual Research Report
  • [Patent(Industrial Property Rights)] タンパク質、ポリヌクレオチド、組換えベクター、形質転換体、ポリエチレンテレフタレート分解用組成物、及びリサイクル品の製造方法2021

    • Inventor(s)
      中村彰彦、飯野亮太
    • Industrial Property Rights Holder
      静岡大学、自然科学研究機構
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-168388
    • Filing Date
      2021
    • Related Report
      2021 Annual Research Report
  • [Funded Workshop] The 1st International Symposium on Molecular Engine2020

    • Related Report
      2019 Annual Research Report

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Published: 2018-07-20   Modified: 2024-01-30  

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