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Structural basis and physico-chemical evaluation for rational design and theoretical calculation of molecular engine

Planned Research

Project AreaMolecular Engine: Design of Autonomous Functions through Energy Conversion
Project/Area Number 18H05425
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Complex systems
Research InstitutionChiba University

Principal Investigator

村田 武士  千葉大学, 大学院理学研究院, 教授 (80415322)

Co-Investigator(Kenkyū-buntansha) 長門石 曉  東京大学, 医科学研究所, 特任准教授 (30550248)
Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Granted (Fiscal Year 2022)
Budget Amount *help
¥125,710,000 (Direct Cost: ¥96,700,000、Indirect Cost: ¥29,010,000)
Fiscal Year 2022: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2021: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2020: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000)
Fiscal Year 2019: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2018: ¥38,870,000 (Direct Cost: ¥29,900,000、Indirect Cost: ¥8,970,000)
Keywords発動分子 / 構造基盤 / 物理化学評価
Outline of Annual Research Achievements

(1)発動分子の物理化学解析(長門石が担当):本年度は、昨年度に引き続きA01班が創出した生体発動分子の各種変異体に関して、DSC測定による熱安定解析にて成果が得られた。変異導入した各種アミノ酸によってDSCのピーク形状が異なることが明らかとなった。多段階の変性が起こることが観察された一方で、導入したアミノ酸の官能基構造の違いがタンパク質内部のコア構造および安定性に影響を及ぼしていることがDSCプロファイルの差異より明らかとなった(論文準備中)。
(2)理論的耐熱化変異体作製技術の開発(村田が担当):本年度は、回転型発動分子の構造固定に寄与しているN末バレルドメインに焦点を当て、理論計算結果を参考に変異体を作製した。得られた変異体は耐熱性が向上するばかりでなく、ATPase活性も向上した。
(3)生体発動分子の耐熱化と構造解析(村田が担当):上記で作製した回転型発動分子の耐熱化変異体の立体構造をX線結晶構造解析により決定した(論文準備中)。これらの構造情報からさらに超機能化した回転型発動分子の創成を目指す。
(4)人工およびハイブリッド発動分子の構造解析(村田が担当):A01班が創造した固定化により蛍光を発する機能分子がATP駆動性薬剤排出ポンプを活性化することを見出した。現在クライオ電子顕微鏡を用いて複合体構造解析を行っている。
(5)発動分子の合理設計指針の提案(長門石、村田が担当):本研究によって得られたタンパク質と有機分子が結合・融合した生体発動分子の物理化学情報・立体構造情報・理論解析情報から本発動分子の合理設計指針を提案した。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

当初計画通り、(1)生体発動分子の各種変異体に関するDSC解析、(2)回転型発動分子の耐熱化変異体の創出、(3)得られた回転型発動分子の耐熱化変異体の立体構造決定、(4)有機分子と発動分子の相互作用解析、(5)有機分子融合型の生体発動分子に関する設計指針の提案を行った。このため予定通り順調に進展していると考えている。

Strategy for Future Research Activity

当初計画に従って、各研究項目を継続する。特に公募班を含めて各班と連携を強化する。これにより各種発動分子に関する合理設計・理論計算するための物理化学評価と構造基盤の確立を行う。

Report

(4 results)
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(142 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (9 results) Journal Article (30 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 30 results,  Open Access: 16 results) Presentation (102 results) (of which Int'l Joint Research: 11 results,  Invited: 19 results) Book (1 results)

  • [Int'l Joint Research] Arizona State University/University of Illinois(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University de Lorraine(フランス)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Arizona State University/University of Illinois(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Medical Research Council/University of Oxford/Imperial College London(英国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University de Lorraine(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Arizona State University/University of Illinois(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University de Lorraine(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Arizona State University/University of Illinois(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University de Lorraine(フランス)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Controlling the Rigidity of Kinesin-Propelled Microtubules in an In Vitro Gliding Assay Using the Deep-Sea Osmolyte Trimethylamine N-Oxide2022

    • Author(s)
      Kabir Arif Md. Rashedul、Munmun Tasrina、Hayashi Tomohiko、Yasuda Satoshi、Kimura Atsushi P.、Kinoshita Masahiro、Murata Takeshi、Sada Kazuki、Kakugo Akira
    • Journal Title

      ACS Omega

      Volume: 7 Pages: 3796-3803

    • DOI

      10.1021/acsomega.1c06699

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discovery of ultrafast myosin, its amino acid sequence, and structural features2022

    • Author(s)
      Haraguchi Takeshi、Tamanaha Masanori、Suzuki Kano、Yoshimura Kohei、Imi Takuma、Tominaga Motoki、Sakayama Hidetoshi、Nishiyama Tomoaki、Murata Takeshi、Ito Kohji
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119

    • DOI

      10.1073/pnas.2120962119

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of an Outward Proton Pumping Rhodopsin with a New Record in Thermostability by Means of Amino Acid Mutations2022

    • Author(s)
      Yasuda Satoshi、Akiyama Tomoki、Kojima Keiichi、Ueta Tetsuya、Hayashi Tomohiko、Ogasawara Satoshi、Nagatoishi Satoru、Tsumoto Kouhei、Kunishima Naoki、Sudo Yuki、Kinoshita Masahiro、Murata Takeshi
    • Journal Title

      J Phys Chem B

      Volume: 126 Pages: 1004-1015

    • DOI

      10.1021/acs.jpcb.1c08684

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural characterization of proton-pumping rhodopsin lacking a cytoplasmic proton donor residue by X-ray crystallography2022

    • Author(s)
      Suzuki Kano、del Carmen Mar?n Maria、Konno Masae、Bagherzadeh Reza、Murata Takeshi、Inoue Keiichi
    • Journal Title

      Journal of Biological Chemistry

      Volume: 298 Pages: 101722-101722

    • DOI

      10.1016/j.jbc.2022.101722

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] State-Targeting Stabilization of Adenosine A2A Receptor by Fusing a Custom-Made De Novo Designed α-Helical Protein2021

    • Author(s)
      Mitsumoto Masaya、Sugaya Kanna、Kazama Kazuki、Nakano Ryosuke、Kosugi Takahiro、Murata Takeshi、Koga Nobuyasu
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Pages: 12906-12906

    • DOI

      10.3390/ijms222312906

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-based screening combined with computational and biochemical analyses identified the inhibitor targeting the binding of DNA Ligase 1 to UHRF12021

    • Author(s)
      Kori Satomi、Shibahashi Yuki、Ekimoto Toru、Nishiyama Atsuya、Yoshimi Sae、Yamaguchi Kosuke、Nagatoishi Satoru、Ohta Masateru、Tsumoto Kouhei、Nakanishi Makoto、Defossez Pierre-Antoine、Ikeguchi Mitsunori、Arita Kyohei
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 52 Pages: 116500-116500

    • DOI

      10.1016/j.bmc.2021.116500

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of cadherin dimerization by chemical fragments as a trigger to inhibit cell adhesion2021

    • Author(s)
      Senoo Akinobu、Ito Sho、Nagatoishi Satoru、Saito Yutaro、Ueno Go、Kuroda Daisuke、Yoshida Kouhei、Tashima Takumi、Kudo Shota、Sando Shinsuke、Tsumoto Kouhei
    • Journal Title

      Communications Biology

      Volume: 4 Pages: 1041-1041

    • DOI

      10.1038/s42003-021-02575-3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of dimerization and structural features of human LI-cadherin2021

    • Author(s)
      Yui Anna、Caaveiro Jose M.M.、Kuroda Daisuke、Nakakido Makoto、Nagatoishi Satoru、Goda Shuichiro、Maruno Takahiro、Uchiyama Susumu、Tsumoto Kouhei
    • Journal Title

      Journal of Biological Chemistry

      Volume: 297 Pages: 101054-101054

    • DOI

      10.1016/j.jbc.2021.101054

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anion solvation enhanced by positive supercharging mutations preserves thermal stability of an antibody in a wide pH range2021

    • Author(s)
      Kasahara Keisuke、Kuroda Daisuke、Tanabe Aki、Kawade Raiji、Nagatoishi Satoru、Tsumoto Kouhei
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 563 Pages: 54-59

    • DOI

      10.1016/j.bbrc.2021.05.053

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Further thermo‐stabilization of thermophilic rhodopsin from Thermus thermophilus JL ‐18 through engineering in extramembrane regions2021

    • Author(s)
      Akiyama Tomoki、Kunishima Naoki、Nemoto Sayaka、Kazama Kazuki、Hirose Masako、Sudo Yuki、Matsuura Yoshinori、Naitow Hisashi、Murata Takeshi
    • Journal Title

      Proteins: Structure, Function, and Bioinformatics

      Volume: 89 Pages: 301-310

    • DOI

      10.1002/prot.26015

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cryo-EM Structure of K+-Bound hERG Channel Complexed with the Blocker Astemizole2021

    • Author(s)
      Asai Tatsuki、Adachi Naruhiko、Moriya Toshio、Oki Hideyuki、Maru Takamitsu、Kawasaki Masato、Suzuki Kano、Chen Sisi、Ishii Ryohei、Yonemori Kazuko、Igaki Shigeru、Yasuda Satoshi、Ogasawara Satoshi、Senda Toshiya、Murata Takeshi
    • Journal Title

      Structure

      Volume: 29

    • DOI

      10.1016/j.str.2020.12.007

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics2021

    • Author(s)
      Shimizu Kouki、Seiki Issei、Goto Yoshiyuki、Murata Takeshi
    • Journal Title

      Antibiotics

      Volume: 10 Pages: 180-180

    • DOI

      10.3390/antibiotics10020180

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thermodynamic Dissection of Potency and Selectivity of Cytosolic Hsp90 Inhibitors2021

    • Author(s)
      Yoshimura Chihoko、Nagatoishi Satoru、Kuroda Daisuke、Kodama Yasuo、Uno Takao、Kitade Makoto、Chong-Takata Khoontee、Oshiumi Hiromi、Muraoka Hiromi、Yamashita Satoshi、Kawai Yuichi、Ohkubo Shuichi、Tsumoto Kouhei
    • Journal Title

      Journal of Medicinal Chemistry

      Volume: 64 Pages: 2669-2677

    • DOI

      10.1021/acs.jmedchem.0c01715

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic identification and validation of novel interactions of the putative tumor suppressor PRELP with membrane proteins including IGFI-R and p75NTR2021

    • Author(s)
      Kosuge Hirofumi、Nakakido Makoto、Nagatoishi Satoru、Fukuda Tetsuya、Bando Yasuhiko、Ohnuma Shin-ichi、Tsumoto Kouhei
    • Journal Title

      Journal of Biological Chemistry

      Volume: 296 Pages: 100278-100278

    • DOI

      10.1016/j.jbc.2021.100278

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Theoretical identification of thermostabilizing amino acid mutations for G-protein-coupled receptors2020

    • Author(s)
      Takeshi Murata、Satoshi Yasuda、Tomohiko Hayashi、Masahiro Kinoshita
    • Journal Title

      Biophysical Reviews

      Volume: - Pages: 323-332

    • DOI

      10.1007/s12551-020-00678-5

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases2020

    • Author(s)
      Nakamura A, Ishiwata D, Visootsat A, Uchiyama T, Mizutani K, Kaneko S, Murata T, Igarashi K, Iino R
    • Journal Title

      J. Biol. Chem.

      Volume: 295 Pages: 14606-14617

    • DOI

      10.1074/jbc.ra120.014792

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structure of an anti-podoplanin antibody bound to a disialylated O-linked glycopeptide2020

    • Author(s)
      Ogasawara S, Suzuki K, Naruchi K, Nakamura S, Shimabukuro J, Tsukahara N, Kaneko MK, Kato Y, Murata T
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 533 Pages: 57-63

    • DOI

      10.1016/j.bbrc.2020.08.103

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An affinity change model to elucidate the rotation mechanism of V1-ATPase2020

    • Author(s)
      Arai Satoshi、Maruyama Shintaro、Shiroishi Mitsunori、Yamato Ichiro、Murata Takeshi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 533 Pages: 1413-1418

    • DOI

      10.1016/j.bbrc.2020.10.033

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly sensitive HPLC analysis and biophysical characterization of N‐glycans of IgG‐Fc domain in comparison between CHO and 293 cells using FcγRIIIa ligand2020

    • Author(s)
      Kosuge Hirofumi、Nagatoishi Satoru、Kiyoshi Masato、Ishii‐Watabe Akiko、Tanaka Toru、Terao Yosuke、Oe Seigo、Ide Teruhiko、Tsumoto Kouhei
    • Journal Title

      Biotechnology Progress

      Volume: 36

    • DOI

      10.1002/btpr.3016

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elucidation of cosolvent effects thermostabilizing water-soluble and membrane proteins2020

    • Author(s)
      Yasuda Satoshi、Kazama Kazuki、Akiyama Tomoki、Kinoshita Masahiro、Murata Takeshi
    • Journal Title

      Journal of Molecular Liquids

      Volume: 301 Pages: 112403-112403

    • DOI

      10.1016/j.molliq.2019.112403

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] How Does a Microbial Rhodopsin RxR Realize Its Exceptionally High Thermostability with the Proton-Pumping Function Being Retained?2020

    • Author(s)
      Hayashi Tomohiko、Yasuda Satoshi、Suzuki Kano、Akiyama Tomoki、Kanehara Kanae、Kojima Keiichi、Tanabe Mikio、Kato Ryuichi、Senda Toshiya、Sudo Yuki、Murata Takeshi、Kinoshita Masahiro
    • Journal Title

      J Phys Chem B

      Volume: 124 Pages: 990-1000

    • DOI

      10.1021/acs.jpcb.9b10700

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methodology for Further Thermostabilization of an Intrinsically Thermostable Membrane Protein Using Amino Acid Mutations with Its Original Function Being Retained2020

    • Author(s)
      Yasuda Satoshi、Akiyama Tomoki、Nemoto Sayaka、Hayashi Tomohiko、Ueta Tetsuya、Kojima Keiichi、Tsukamoto Takashi、Nagatoishi Satoru、Tsumoto Kouhei、Sudo Yuki、Kinoshita Masahiro、Murata Takeshi
    • Journal Title

      J Chem Inf Model.

      Volume: 60 Pages: 1709-1716

    • DOI

      10.1021/acs.jcim.0c00063

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-molecule analysis reveals rotational substeps and chemo-mechanical coupling scheme of Enterococcus hirae V1-ATPase2019

    • Author(s)
      Iida Tatsuya、Minagawa Yoshihiro、Ueno Hiroshi、Kawai Fumihiro、Murata Takeshi、Iino Ryota
    • Journal Title

      J. Biol. Chem.

      Volume: 294 Pages: 17017-17030

    • DOI

      10.1074/jbc.ra119.008947

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Application of High-Sensitivity UV photoemission Spectroscopy to Examine the Electronic Structure of Thermophilic Rhodopsin2019

    • Author(s)
      Sano Daisuke、Ide Ichiro、Akiyama Tomoki、Tanaka Yuya、Sudo Yuki、Murata Takeshi、Ishii Hisao
    • Journal Title

      Molecular Crystals and Liquid Crystals

      Volume: 687 Pages: 34-39

    • DOI

      10.1080/15421406.2019.1648052

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metastable asymmetrical structure of shaftless V1 motor2019

    • Author(s)
      S. Maruyama, K. Suzuki, M. Imamura, H. Sasaki, H. Matsunami, K. Mizutani, Y. Saito, F. L. Imai, Y. Ishizuka-Katsura, T. Kimura-Someya, M. Shirouzu, T. Uchihashi, T. Ando, I. Yamato, T. Murata
    • Journal Title

      Science Advances

      Volume: 5

    • DOI

      10.1126/sciadv.aau8149

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rotational Mechanism Model of the Bacterial V1 Motor Based on Structural and Computational Analyses2019

    • Author(s)
      Singharoy A, Chipot C, Ekimoto T, Suzuki K, Ikeguchi M, Yamato I, Murata T.
    • Journal Title

      Frontiers in Physiology

      Volume: 10 Pages: 1-12

    • DOI

      10.3389/fphys.2019.00046

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analyses based on statistical thermodynamics for large difference between thermophilic rhodopsin and xanthorhodopsin in terms of thermostability2019

    • Author(s)
      Yasuda Satoshi、Hayashi Tomohiko、Kajiwara Yuta、Murata Takeshi、Kinoshita Masahiro
    • Journal Title

      Journal of Chemical Physics

      Volume: 150 Pages: 055101-055101

    • DOI

      10.1063/1.5082217

    • NAID

      40022492153

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy2018

    • Author(s)
      Tsunoda J., Song C., Imai F. L., Takagi J., Ueno H., Murata T., Iino R., Murata K.
    • Journal Title

      Sci Rep

      Volume: 8 Pages: 15632-15632

    • DOI

      10.1038/s41598-018-33977-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular basis for governing the morphology of type-I collagen fibrils by Osteomodulin2018

    • Author(s)
      Takumi Tashima , Satoru Nagatoishi, Jose M.M. Caaveiro, Makoto Nakakido, Hiroshi Sagara, Osamu Kusano-Arai, Hiroko Iwanari, Hitomi Mimuro, Takao Hamakubo, Shin-ichi Ohnuma and Kouhei Tsumoto
    • Journal Title

      Commun Biol

      Volume: 1:33 Pages: 1-10

    • DOI

      10.1038/s42003-018-0038-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inhibition of homophilic dimerization and disruption of cell adhesion by P-cadherin-specific small molecules from SPR-based assays2018

    • Author(s)
      Senoo A, Nagatoishi S, Moberg A, Babol LN, Mitani T, Tashima T, Kudo S, Tsumoto K.
    • Journal Title

      Chem. Commun.

      Volume: 54 Pages: 5350-5353

    • DOI

      10.1039/c8cc01964a

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 様々なIgG型抗体に関するFcγRIIIaの物理化学的相互作用解析2022

    • Author(s)
      長門石曉, 小菅啓史, 木吉真人, 石井明子, 寺尾陽介, 井出輝彦, 津本浩平
    • Organizer
      日本薬学会第142年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 熱力学および速度論を基点とした蛋白質を制御する低分子リガンドの開発2022

    • Author(s)
      長門石曉
    • Organizer
      日本化学会 第102春季年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 膜タンパク質の構造解析・機能解析のためのアフィニティ精製システムの構築2021

    • Author(s)
      小笠原 諭、稲村 光亮、陳 思思、中川 史、安田 賢司、村田 武士
    • Organizer
      第21回日本蛋白質科学年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 融合パートナータンパク質のデノボデザインによる GPCR の状態選択的安定化2021

    • Author(s)
      三本 斉也、菅谷 幹奈、風間 一輝、中野 僚介、小杉 貴洋、村田 武士、 古賀 信康
    • Organizer
      第21回日本蛋白質科学年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 膜蛋白質の抗体アフィニティ精製システムの構築2021

    • Author(s)
      小笠原 諭、陳 思思、中川 史、渋谷 南美、安田 賢司、村田 武士
    • Organizer
      量子生命科学会第3回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] G タンパク質共役型受容体を熱安定化するアミノ酸置換の理論的予測法の開発2021

    • Author(s)
      安田賢司、林智彦、小笠原 諭、村田 武士、木下正弘
    • Organizer
      量子生命科学会第3回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] G タンパク質共役型受容体を熱安定化するアミノ酸置換の理論的予測法の開発2021

    • Author(s)
      菅谷 幹奈 安田 賢司、佐藤 愼吾、藤木祐美、陳 思思、小笠原 諭、木下 正弘、村田 武士
    • Organizer
      第 2 回 発動分子科学研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Design schemes for peptides imitating β-hairpin conformation of VHH CDR32021

    • Author(s)
      山本 晃一、中木戸 誠、黒田 大祐、長門石 曉、森本 淳平、山東 信介、津本 浩平
    • Organizer
      2021年度医科研発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] M.R.S.A: fighting with antibodies2021

    • Author(s)
      Valenciano Bellido S, Caaveiro J.M.M, Nakakido M, Nagatoishi S, Tsumoto K.
    • Organizer
      2021年度医科研発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Bioengineering of antibodies: the double antibody2021

    • Author(s)
      Valenciano S, Caaveiro J.M.M, Nakagawa I, Nakakido M, Nagatoishi S,Tsumoto K
    • Organizer
      UoT-MaC-UT workshop
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 抗体表面への正荷電残基変異導入による抗体のpH非依存的な熱安定性獲得機構2021

    • Author(s)
      笠原 慶亮、黒田 大祐、河出 来時、田部 亜季、長門石 曉、津本 浩平
    • Organizer
      第59回 日本生物物理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] aHUSに関連する補体抑制因子CFHと診断薬取得に向けた特異的抗体の機能解析2021

    • Author(s)
      横尾 尚典、田部 亜季、中木戸 誠、長門石 曉、カアベイロ ホセ、吉田 瑶子、池田 洋一郎、南学 正臣、津本 浩平
    • Organizer
      第21回 日本蛋白質科学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] A novel homodimerization mechanism of LI-cadherin2021

    • Author(s)
      nna YuiJose M. M. Caaveiro, Daisuke Kuroda, Makoto Nakakido, Satoru Nagatoishi, Takahiro Maruno, Susumu Uchiyama, and Kouhei Tsumoto
    • Organizer
      The Protein Society 35th Annual Symposium
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プロテオーム解析に基づく腫瘍抑制蛋白質PRELPと膜蛋白質の新規相互作用探索と機能解析2021

    • Author(s)
      小菅 啓史、中木戸 誠、長門石 曉、福田 哲也、板東 泰彦、大沼 信一、津本 浩平
    • Organizer
      第94回 日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Analysis of the molecular mechanisms involved in the recognition of antigen and the regulation of function by antibodies2021

    • Author(s)
      氏家 寛、田部 亜季、長門石 曉、津本 浩平
    • Organizer
      第44回 日本分子生物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Fragment-based screening and hit validation targeting enzymes and protein-protein interactions by SPR and ITC2021

    • Author(s)
      Satoru Nagatoishi, Kouhei Tsumoto
    • Organizer
      PACIFICHEM 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 滴定型熱量測定2021

    • Author(s)
      長門石 曉
    • Organizer
      第7回蛋白質工学研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Interaction analysis between small-molecules and proteins using HDX-MS in physicochemical screening2021

    • Author(s)
      Satoru NAGATOISHI, Kouhei TSUMOTO
    • Organizer
      第69回質量分析総合討論会
    • Related Report
      2021 Annual Research Report
  • [Presentation] バンコマイシン耐性腸球菌V-ATPaseの生化学2021

    • Author(s)
      村田武士
    • Organizer
      第94回日本細菌学会総会シンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 化膿連鎖球菌由来金属獲得蛋白質MtsAに対する機能阻害剤の探索2021

    • Author(s)
      中木戸 誠、竹内 美結、長門石 曉、相川 知宏、中川 一路、津本 浩平
    • Organizer
      第94回 日本細菌学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Structure of the voltage-dependent potassium channel (hERG) using cryo-electron microscopy2020

    • Author(s)
      Tatsuki Asai, Kano Suzuki, Naruhiko Adachi, Masato Kawasaki, Toshio Moriya, Toshiya Senda, Satoshi Ogasawara, Takeshi Murata
    • Organizer
      第58回日本生物物理学会年会 (ポスター発表)
    • Related Report
      2020 Annual Research Report
  • [Presentation] Single-molecule analysis of rotation of Enterococcus hirae V-ATPase depending on sodium ion concentration2020

    • Author(s)
      Akihiro Otomo, Tatsuya Iida, Hiroshi Ueno, Takeshi Murata, Ryota Iino
    • Organizer
      第58回日本生物物理学会年会 (ポスター発表)
    • Related Report
      2020 Annual Research Report
  • [Presentation] V1-ATPaseの回転メカニズムを明らかにする親和性変化モデル2020

    • Author(s)
      丸山 慎太郎、新井 聡、白石 充典、山登 一郎、村田 武士
    • Organizer
      日本生体エネルギー研究会第46回討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 蛋白質-蛋白質間相互作用の速度論的制御に基づくP-カドヘリンの細胞接着形成阻害2020

    • Author(s)
      妹尾 暁暢、長門石 曉、黒田 大祐、伊藤 翔、上野 剛、齋藤 雄太朗、吉田 浩平、田島 卓実、工藤 翔太、山東 信介、津本 浩平
    • Organizer
      第14回バイオ関連化学シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] aHUSに関連する補体抑制因子CFHと特異的抗体の機能解析2020

    • Author(s)
      横尾 尚典、田部 亜季、中木戸 誠、長門石 曉、吉田 瑶子、池田 洋一郎、南学 正臣、津本 浩平
    • Organizer
      第93回 日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 計算デザイン Supercharging 抗体の物性機能解析2020

    • Author(s)
      笠原 慶亮、黒田 大祐 、河出 来時 、田部 亜季 、長門石 曉 、津本 浩平
    • Organizer
      第48回 構造活性相関シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] HDX-MSを活用したP-カドヘリンのホモ複合体に対する低分子阻害剤開発2020

    • Author(s)
      妹尾 暁暢、長門石 曉、伊藤 翔、上野 剛、津本 浩平
    • Organizer
      第16回 日本臨床プロテオゲノミクス研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Proteomic Identification And Validation Of New Interactions Of Novel Tumor Suppressor PRELP With Membrane Proteins2020

    • Author(s)
      Kosuge H, Nakakido M, Nagatoishi S, Fukuda T, Bando Y, Ohnuma S, Tsumoto K
    • Organizer
      第20回 日本蛋白質科学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 化膿連鎖球菌の金属獲得蛋白質MtsAに対する機能阻害剤の探索2020

    • Author(s)
      竹内 美結、中木戸 誠、長門石 曉、河出 来時、相川 知宏、中川 一路、津本 浩平
    • Organizer
      第93回 日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Inhibition of P-cadherin-mediated cell contact formation through the kinetic effect of chemical fragment2020

    • Author(s)
      妹尾 暁暢、齋藤 雄太朗、伊藤 翔、長門石 曉、上野 剛、吉田 浩平、田島 卓実、工藤 翔太、山東 信介、津本 浩平
    • Organizer
      第20回 日本蛋白質科学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The Molecular Basis of How SNP in LI-cadherin Affected Cancer Metastasis2020

    • Author(s)
      由井 杏奈、菊池 智佳、郷田 秀一郎、工藤 翔太、秋葉 宏樹、中木戸 誠、黒田大祐、長門石 曉
    • Organizer
      第20回 日本蛋白質科学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular basis of increased risk of colorectal cancer metastasis caused by SNPs in LI-cadherin2020

    • Author(s)
      由井 杏奈、菊池 智佳、郷田 秀一郎、丸野 孝浩、内山 進、中木戸 誠、黒田大祐、長門石 曉、新井 修、岩成 宏子、浜窪 隆雄、津本 浩平
    • Organizer
      第58回 日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] VHH抗体β-hairpin型CDR3を利用したペプチド設計手法の探索2020

    • Author(s)
      山本晃一、中木戸 誠、黒田 大祐、長門石 曉、森本 淳平、山東 信介、津本 浩平
    • Organizer
      第43回 日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Development of new antibacterial drug targeting V-ATPase in Vancomycin-resistant Enterococcus2019

    • Author(s)
      Takeshi Murata
    • Organizer
      Gordon Research Conference on Bioenergetics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 統計熱力学から理解する膜タンパク質の熱安定性の原理2019

    • Author(s)
      村田武士
    • Organizer
      第19回蛋白質科学会・第71回細胞生物学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 耐熱性ロドプシンの立体構造と耐熱化メカニズム2019

    • Author(s)
      村田武士
    • Organizer
      ISSPワークショップ
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 回転分子モータータンパク質によるプロトン輸送メカニズム2019

    • Author(s)
      村田武士
    • Organizer
      第92回日本生化学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Rotational molecular mechanism of bacterial V1-ATPase2019

    • Author(s)
      Takeshi Murata
    • Organizer
      2nd Tokyo ATPase Workshop
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 素人でもなんとかなるクライオEM2019

    • Author(s)
      村田武士
    • Organizer
      日本分子生物学会フォーラム企画
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 多剤耐性腸球菌V-ATPaseを標的とした新規抗菌薬の開発2019

    • Author(s)
      村田武士
    • Organizer
      第2回構造生命科学研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 薬剤耐性腸球菌V-ATPaseを標的とした新規抗菌薬の開発2019

    • Author(s)
      清水 康輝、森山 克彦、後藤 義幸、村田 武士
    • Organizer
      日本生体エネルギー研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] バンコマイシン耐性腸球菌に存在するV-ATPaseを分子標的とした新規抗菌薬の開発2019

    • Author(s)
      清水 康輝、薬師寺 リカ ファビアナ、森山 克彦、後藤 義幸、村田 武士
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Inhibition of V-ATPase decrease the growth of Vancomycin-resistant Enterococcus (VRE) specifically2019

    • Author(s)
      Kouki Shimizu, Fabiana Lica Yakushiji, Katsuhiko Moriyama, Yoshiyuki Goto, Takeshi Murata
    • Organizer
      第2回AMED薬剤耐性シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Na+-transporting V-ATPase is a new target for drug development against Vancomycin-resistant Enterococcus (VRE)2019

    • Author(s)
      Kouki Shimizu, Fabiana Lica Yakushiji, Katsuhiko Moriyama, Yoshiyuki Goto, Takeshi Murata
    • Organizer
      Gordon Research Conference on Bioenergetics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural insights into the subtype-selective antagonist binding to the M2 muscarinic receptor2019

    • Author(s)
      Ryoji Suno, Sangbae Lee, Shoji Maeda, Satoshi Yasuda, Keitaro Yamashita, Kunio Hirata, Takeshi Murata, Masahiro Kinoshita, Masaki Yamamoto, Brian Kobilka, Nagarajan Vaidehi, So Iwata, Takuya Kobayashi
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Theoretical Prediction of Thermostabilizing Mutations for GPCR: Identification of Hot-Spot Residues to be Mutated Common in Class A GPCRs2019

    • Author(s)
      Satoshi Yasuda, Yuta Kajiwara, Yuki Takamuku, Nanao Suzuki, Yosuke Toyoda, Kazushi Morimoto, Ryoji Suno, So Iwata, Takuya Kobayashi, Takeshi Murata, Masahiro Kinoshita
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The combination of high-speed atomic force microscopy and X-ray crystallography reveals rotary catalysis of a shaftless V1 motor2019

    • Author(s)
      Shintaro Maruyama, Motonori Imamura, Takayuki Uchihashi, Kazuya Nakamoto, Mizutani Kenji, Lica Fabiana Imai, Kano Suzuki, Yoshiko Ishizuka-Katsura, Tomomi Someya-Kimura, Mikako Shirouzu, Ichiro Yamato, Toshio Ando, Takeshi Murata
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Further Thermo-Stabilization of Thermophilic Rhodopsin through Engineering in Intramembrane and Extramembrane Regions2019

    • Author(s)
      Tomoki Akiyama, Naoki Kunishima, Masako Hirose, Sayaka Nemoto, Stoshi Yasuda, Yuki Sudo, Takeshi Murata
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Tailor-made design of superstable proteins from scratch for rational stabilization of GPCR2019

    • Author(s)
      Masaya Mitsumoto, Nanao Suzuki, Ryosuke Nakano, Takahiro Kosugi, Takeshi Murata, Nobuyasu Koga
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Chemo-mechanical coupling scheme of rotary molecular motor Enterococcus hirae V1-ATPase revealed by single-molecule analysis2019

    • Author(s)
      Tatsuya Iida, Yoshihiro Minagawa, Hiroshi Ueno, Fumihiro Kawai, Takeshi Murata, Ryota Iino
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The off-axis rotor of Enterococcus hirae V-type ATPase by Volta phase contrast cryo-EM2019

    • Author(s)
      Burton-Smith Raymond. N, Tsunoda Jun, Yamamori Yu, Miyazaki Naoyuki, Imai Fabiana L., Song Chihong, Tomii Kentaro, Iwasaki Kenji, Takagi Junichi, Ueno Hiroshi, Murata Takeshi, Iino Ryota, Murata Kazuyoshi
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The influence of cosolvent on thermal stability of membrane proteins2019

    • Author(s)
      Kazuki Kazama, Satoshi Yasuda, Tomoki Akiyama, Masahiro Kinoshita, Takeshi Murata
    • Organizer
      CBI学会2019年大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development of the screening system to create GPCR mutants stabilized in active states2019

    • Author(s)
      Ryosuke Nakano, Nanao Suzuki, Simon Hikiri, Satoshi Yasuda, Mitsunori Shiroishi, Masahiro Kinoshita, Takeshi Murata
    • Organizer
      CBI学会2019年大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Analyses based on statistical thermodynamics for large difference between thermophilic rhodopsin and xanthorhodopsin in terms of thermostability2019

    • Author(s)
      Satoshi Yasuda, Tomohiko Hayashi, Yuta Kajiwara, Takeshi Murata, Masahiro Kinoshita
    • Organizer
      CBI学会2019年大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Calorimetric Selection of Ligands to Inhibit the Protein Functions in Cell and in Vivo2019

    • Author(s)
      Satoru Nagatoishi, Kouhei Tsumoto
    • Organizer
      The Seventh International Symposium on the New Frontiers of Thermal Studies of Materials
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Thermodynamic selection of ligands to inhibit the protein functions in cell and in vivo2019

    • Author(s)
      Satoru Nagatoishi, Kouhei Tsumoto
    • Organizer
      The 7th International Postgraduate Conference on Pharmaceutical Sciences
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Elucidation of the interactions for natural flexible and unstable proteins using HDX-MS2019

    • Author(s)
      長門石曉、津本浩平
    • Organizer
      第65回質量分析総合討論会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 生体分子を制御する特異的リガンドの物理化学的探索と解析2019

    • Author(s)
      長門石曉、津本浩平
    • Organizer
      生有研シンポジウム 2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Disruption of Homophilic Protein-protein Interaction of P-cadherin by A Fragment Compound as A Trigger To Inhibit Cell Adhesion2019

    • Author(s)
      妹尾 暁暢、長門石 曉、吉田 浩平、伊藤 翔、上野 剛、田島 卓実、工藤 翔太、津本 浩平
    • Organizer
      33rd The Protein Society Annual Symposium
    • Related Report
      2019 Annual Research Report
  • [Presentation] Biophysics of the Protein-ligand Interactions to Regulate the Protein Function2019

    • Author(s)
      S Nagatoishi、K Tsumoto
    • Organizer
      10th RSC-CSJ Joint Symposium
    • Related Report
      2019 Annual Research Report
  • [Presentation] 医薬品設計の提案を指向したHDX-MSによる蛋白質間相互作用解析2019

    • Author(s)
      長門石曉、 展天承、 森千夏、 中木戸誠、 津本浩平
    • Organizer
      第15回日本臨床プロテオゲノミクス研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低分子リガンドによる細胞接着蛋白質P-カドヘリンの同種親和的二量体化阻害に対する分子機構解明2019

    • Author(s)
      妹尾暁暢、長門石曉、吉田浩平、伊藤翔、上野剛、田島卓実、工藤翔太、津本浩平
    • Organizer
      第19回 日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] S100A4-MetAP2蛋白質間相互作用に対するペプチド阻害剤の相互作用解析2019

    • Author(s)
      片桐直宏、長門石曉、遠藤英也、津本浩平
    • Organizer
      第19回 日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 小分子リガンドによる細胞接着蛋白質P-カドヘリンの分子間相互作用制御と構造情報に基づくリガンド設計2019

    • Author(s)
      妹尾暁暢、齋藤雄太朗、伊藤翔、長門石曉、上野剛、吉田浩平、田島卓実、工藤翔太、山東信介、津本浩平
    • Organizer
      第13回バイオ関連化学シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] ホモ二量体形成蛋白質P-カドヘリンによる細胞接着阻害を可能にする新規低分子リガンド結合部位の同定2019

    • Author(s)
      妹尾暁暢、長門石曉、吉田浩平、伊藤翔、上野剛、田島卓実、工藤翔太、津本浩平
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] S100A4-MetAP2間相互作用に対するHelix-turn-helix構造を形成するペプチド 阻害剤の解析2019

    • Author(s)
      片桐直宏、長門石曉、遠藤英也、津本浩平
    • Organizer
      第37回 メディシナルケミストリーシンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 物理化学的手法を駆使した細胞接着蛋白質P-カドヘリンのホモ二量体化相互作用を阻害する低分子の同定とその阻害機構解明2019

    • Author(s)
      妹尾暁暢、齋藤雄太朗、伊藤翔、長門石曉、上野剛、吉田浩平、田島卓実、工藤翔太、山東信介、津本浩平
    • Organizer
      第37回 メディシナルケミストリーシンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] New methodologies for human membrane protein crystallography2019

    • Author(s)
      Takeshi Murata
    • Organizer
      Gordon Research Conference on IGF and Insulin System in Physiology and Disease
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 蛋白質-リガンド, 抗原-抗体相互作用解析における熱分析の活用2019

    • Author(s)
      長門石曉、津本浩平
    • Organizer
      熱測定スプリングスクール2019
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Further thermostabilization of thermophilic rhodopsin TR2018

    • Author(s)
      Takeshi Murata
    • Organizer
      23th Biophysics Conference in Taiwan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 理論計算を用いた膜蛋白質の耐熱化法の開発2018

    • Author(s)
      村田 武士
    • Organizer
      第4回蛋白質工学研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Further thermostabilization of Microbial rhodopsins2018

    • Author(s)
      Takeshi Murata
    • Organizer
      The 79th Okazaki Conference
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] X線結晶構造解析を用いたシャフトレスV1-ATPaseの非対称構造形成機構の探索2018

    • Author(s)
      丸山 慎太郎、鈴木 花野、今村 元紀、佐々木 輝、松波 秀行、水谷 健二、齋藤 靖子、今井 ファビアナ・リカ、石塚(桂)芳子、 染谷(木村)友美、白水 美香子、内橋 貴之、安藤 敏夫、山登 一郎、村田 武士
    • Organizer
      量子生命科学研究会第2回学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] N 末端側βバレルドメインのクラウン構造が非対称性を生み、恊動性を付与する2018

    • Author(s)
      丸山 慎太郎、鈴木 花野、今村 元紀、佐々木 輝、松波 秀行、水谷 健二、齋藤 靖子、今井 ファビアナ・リカ、石塚(桂)芳子、 染谷(木村)友美、白水 美香子、内橋 貴之、安藤 敏夫、山登 一郎、村田 武士
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Immobilization of conformational state of E.hirae V-ATPase complex using PA tag system for structural analysis2018

    • Author(s)
      Fabiana L Yakushiji, Jun Tsunoda, Chihong Song, Hiroshi Ueno, Ryota Iino, Satoshi Ogasawara, Junichi Takagi, Kazuyoshi Murata, Takeshi Murata
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] all-αタンパク質のデザイン2018

    • Author(s)
      佐久間 航也、杉木 俊彦、鈴木 花野、小林 直也、小杉 貴洋、小林 直宏、 村田 武士、古賀 理恵、古賀 信康
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大腸菌発現系を用いたヒト由来 A2Aアデノシン受容体の発現・精製検討2018

    • Author(s)
      鈴木 七緒、高椋 勇樹、吉田 千香、村田 武士
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] G タンパク質共役受容体の不安定領域の再デザイン2018

    • Author(s)
      三本 斉也、村田 武士、古賀 信康
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Chemo-mechanical coupling scheme of rotary molecular motor V1-ATPase revealed by single-molecule analysis2018

    • Author(s)
      Tatsuya Iida, Yoshihiro Minagawa, Hiroshi Ueno, Fumihiro Kawai, Takeshi Murata, and Ryota Iino
    • Organizer
      The 79th Okazaki Conference
    • Related Report
      2018 Annual Research Report
  • [Presentation] Statistical thermodynamics on the large difference between thermophilic rhodopsin and xanthorhodopsin in terms of thermostability2018

    • Author(s)
      Satoshi Yasuda, Tomohiko Hayashi, Yuta Kajiwara, Takeshi Murata, and Masahiro Kinoshita
    • Organizer
      The 79th Okazaki Conference
    • Related Report
      2018 Annual Research Report
  • [Presentation] Design of all-α proteins2018

    • Author(s)
      Koya Sakuma, Naohiro Kobayashi, Kano Suzuki, Toshihiko Sugiki, Naoya Kobayashi, Takahiro Kosugi, Takeshi Murata, Toshimichi Fujiwara, Rie Koga, Nobuyasu Koga
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Electronic structure of a film with TR observed by technique using photoelectron emission 2: Comparison with RxR2018

    • Author(s)
      Daisuke Sano, Yuki Takeda, Tomoki Akiyama, Kanae Kanahara, Takeshi Murata, Yuki Sudo, Hisao Ishi
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Gap state of lysozyme thin film observed by high-sensitivity UV photoelectron spectroscopy2018

    • Author(s)
      Ichiro Ide, Daisuke Sano, Shintaro Maruyama, Yuya Tanaka, Takeshi Murata, Hisao Ishii
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Physical origin of exceptionally high thermostability of thermophilic rhodopsin2018

    • Author(s)
      Satoshi Yasuda, Tomohiko Hayashi, Yuta Kajiwara, Takeshi Murata, Masahiro Kinoshita
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Thermostabilization of the microbial rhodopsin TR by carotenoid binding and amino-acid mutation2018

    • Author(s)
      Tomoki Akiyama, Keigo Nishikawa, Sayaka Nemoto, Satoshi Yasuda, Daisuke Umeno, Masahiro Kinoshita, Yuki Sudo, Takeshi Murata
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Statistical thermodynamics for the extremely high thermostability of a microbial rhodopsin from the eubacterium Rubrobacter (RxR)2018

    • Author(s)
      Tomohiko Hayashi, Satoshi Yasuda, Kano Suzuki, Tomoki Akiyama, Kanae Kanehara, Yuki Sudo, Takeshi Murata, Masahiro Kinoshita
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Rational Design of G-Protein Coupled Receptors Stabilized in Aimed State2018

    • Author(s)
      Masaya Mitsumoto, Ryosuke Nakano, Takeshi Murata, Nobuyasu Koga
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Efficient screening system construction to create thermo-stabilized GPCR mutants using theoretical calculation and evolutional engineering2018

    • Author(s)
      Shingo Sato, Nanao Suzuki, Satoshi Yasuda, Yuta Kaziwara, Hiroki Nakai, Kinuka Isshiki, Tomoaki Matsuura, Masahiro Kinoshita, Takeshi Murata
    • Organizer
      日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 理論計算と進化分子工学を融合したGPCR安定化変異体スクリーニング系の構築2018

    • Author(s)
      佐藤愼吾、鈴木七緒、梶原佑太、安田賢司、仲井宏紀、一色衣香、松浦友亮、木下正弘、村田武士
    • Organizer
      日本生体エネルギー研究会 第44回討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] GPCR活性型変異体スクリーニング系の構築2018

    • Author(s)
      中野僚介、肥喜里志門、安田賢司、白石充典、木下正弘、村田武士
    • Organizer
      日本生体エネルギー研究会 第44回討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メラトニン1A受容体の高発現・熱安定化改変体の創出2018

    • Author(s)
      扇谷和希、佐藤愼吾、鈴木七緒、安田賢司、白石充典、木下 正弘、村田武士
    • Organizer
      日本生体エネルギー研究会 第44回討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] N末端βバレルクラウンがV1モーターの非対称構造形成に寄与する2018

    • Author(s)
      丸山 慎太郎、鈴木 花野、今村 元紀、佐々木 輝、松波 秀行、水谷 健二、齋藤 靖子、イマイ ファビアナ リカ、石塚(桂) 芳子、木村(染谷) 友美、白水 美香子、内橋 貴之、安藤敏夫、山登 一郎、村田 武士
    • Organizer
      日本生体エネルギー研究会 第44回討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Physicochemical and Computational Analysis for S100A4-MetAP2 peptide Interaction2018

    • Author(s)
      Naohiro Katagiri, Satoru Nagatoishi, Hideya Endo, Kouhei Tsumoto
    • Organizer
      sian Biophysics Association Symposium in conjunction with the Australian Society for Biophysics Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 天然変性タンパク質のフラグメント化によるタンパク質間相互作用阻害リガンド探索2018

    • Author(s)
      長門石曉、津本浩平
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞内の蛋白質間相互作用を制御する物理化学的リガンド探索2018

    • Author(s)
      長門石曉、津本浩平
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Nanodisc-GPCRを用いた物理化学的アプローチによる低分子薬剤探索2018

    • Author(s)
      吉田 浩平、長門石 曉、黒田 大祐、津本 浩平
    • Organizer
      第9回スクリーニング学研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] SPRを用いた化膿連鎖球菌の宿主感染関連PPIを阻害する天然物化合物の探索2018

    • Author(s)
      妹尾 暁暢、長門石 曉、中木戸 誠、星野 将人、中山 登、坂東 泰彦、石崎 仁將、澤 竜一、五十嵐 雅之、津本 浩平
    • Organizer
      第9回スクリーニング学研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] LI-cadherinのTrp残基への変異導入がホモ二量体形成に与える影響2018

    • Author(s)
      由井 杏奈、菊池 智佳、郷田 秀一郎、工藤 翔太、秋葉 宏樹、中木戸 誠、長門石 曉、新井 修、岩成 宏子、浜窪 隆雄、津本 浩平
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Nanodisc を用いたリン脂質二重膜環境中におけるヒトセロトニン受容体の機能解析2018

    • Author(s)
      吉田 浩平、黒田 大祐、長門石 曉、津本 浩平
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 物理化学測定を用いた異なる脂質環境におけるセロトニン受容体の機能評価2018

    • Author(s)
      吉田 浩平、長門石 曉、津本 浩平
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] SPRを活用した鉄獲得機構を標的とした化膿連鎖球菌に対する新規抗菌剤の探索2018

    • Author(s)
      妹尾 暁暢、長門石 曉、福田 哲也、小尾 奈緒子、中山 登、坂東 泰彦、石崎 仁將、澤 竜一、五十嵐 雅之、津本 浩平
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] SAXS及びITCによるホモ二量体形成蛋白質LI-cadherinの物性解析2018

    • Author(s)
      由井 杏奈、菊池 智佳、郷田 秀一郎、工藤 翔太、秋葉 宏樹、中木戸 誠、長門石 曉、新井 修、岩成 宏子、浜窪 隆雄、津本 浩平
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Annual Research Report
  • [Book] 創薬研究のための相互作用解析パーフェクト2021

    • Author(s)
      長門石曉、津本浩平
    • Total Pages
      368
    • Publisher
      羊土社
    • ISBN
      4758122563
    • Related Report
      2021 Annual Research Report

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Published: 2018-07-20   Modified: 2022-12-28  

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