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Imaging analysis on cell geometry during mechanical optimization in leaves

Planned Research

Project AreaElucidation of the strategies of mechanical optimization in plants toward the establishment of the bases for sustainable structure system
Project/Area Number 18H05492
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Complex systems
Research InstitutionKumamoto University

Principal Investigator

Higaki Takumi  熊本大学, 大学院先端科学研究部(理), 教授 (90578486)

Co-Investigator(Kenkyū-buntansha) 加藤 壮英  奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (70379535)
國田 樹  琉球大学, 工学部, 准教授 (20645478)
Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥57,460,000 (Direct Cost: ¥44,200,000、Indirect Cost: ¥13,260,000)
Fiscal Year 2022: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2021: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2020: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2019: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2018: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Keywordsジョイント構造 / 継手 / 画像解析 / 葉表皮細胞 / バイオイメージング / 継ぎ手
Outline of Final Research Achievements

In this study, we investigated the structural significance of the jigsaw puzzle-like morphogenesis of leaf epidermal cells, and explored the potential application of the cell biological structure to artificial structures. Our analysis indicated that the jigsaw puzzle-like cell shape moderates local growth throughout the organ, which may affect the formation of the round fan-shaped morphology of the cotyledons. Furthermore, we found that the cell wall waving pattern observed in pavement cells could be applied to the structure of joints. We also developed an experimental system to non-destructively reconstruct the three-dimensional morphology of above-ground parts such as flower stems and lateral branches of Arabidopsis thaliana, and to measure the angular and curvature distribution of lateral branches. Using this system, we analyzed temporal changes in lateral branch morphology of myosin XI mutants, suggesting that myosin X contributes to the upward growth of lateral branches.

Academic Significance and Societal Importance of the Research Achievements

本研究では、葉の細胞がなぜジグソーパズルのような形に変形するのか、その理由を探求した。研究の結果、この細胞の形が葉の形状を保つのに役立つ場合があることがわかった。この研究成果は、自然のデザインがどのように機能するかを理解する助けとなり、その知識を建築などの人工構造に応用する可能性が開かれた。また、私たちは草姿を立体的に再構成し、その成長を追跡する方法を開発した。これにより、特定の遺伝子が植物の成長にどのように影響を与えるかを理解することが可能になった。以上の成果は、自然のデザイン原理を理解し、それを実生活に適用することで、持続可能な社会を構築する手がかりを提供するものである。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (64 results)

All 2023 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (40 results) (of which Int'l Joint Research: 14 results,  Peer Reviewed: 40 results,  Open Access: 32 results) Presentation (16 results) (of which Int'l Joint Research: 5 results,  Invited: 13 results) Remarks (6 results)

  • [Int'l Joint Research] UC Davis/Michigan State University(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of California, Davis(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Ab-GALFA, A bioassay for insect gall formation using the model plant Arabidopsis thaliana2023

    • Author(s)
      Tomoko Hirano , Ayaka Okamoto , Yoshihisa Oda , Tomoaki Sakamoto , Seiji Takeda , Takakazu Matsuura , Yoko Ikeda , Takumi Higaki , Seisuke Kimura , Masa H Sato
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1 Pages: 2554-2554

    • DOI

      10.1038/s41598-023-29302-8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Migration of prospindle before the first asymmetric division in germinating spore of <i>Marchantia polymorpha</i>2022

    • Author(s)
      Sakai Yuuki、Higaki Takumi、Ishizaki Kimitsune、Nishihama Ryuichi、Kohchi Takayuki、Hasezawa Seiichiro
    • Journal Title

      Plant Biotechnology

      Volume: 39 Issue: 1 Pages: 5-12

    • DOI

      10.5511/plantbiotechnology.21.1217b

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2022-03-25
    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tracking metabolites at single-cell resolution reveals metabolic dynamics during plant mitosis.2022

    • Author(s)
      Okubo-Kurihara E, Ali A, Hiramoto M, Kurihara Y, Abouleila Y, Abdelazem EM, Kawai T, Makita Y, Kawashima M, Esaki T, Shimada H, Mori T, Hirai MY, Higaki T, Hasezawa S, Shimizu Y, Masujima T, Matsui M
    • Journal Title

      Plant Physiol

      Volume: 189 Issue: 2 Pages: 459-464

    • DOI

      10.1093/plphys/kiac093

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two Kinesin-14A motors oligomerize to drive poleward microtubule convergence for acentrosomal spindle morphogenesis in Arabidopsis thaliana.2022

    • Author(s)
      Hotta T, Lee YR, Higaki T, Hashimoto T, Liu B
    • Journal Title

      Front Cell Dev Biol.

      Volume: 10 Pages: 949345-949345

    • DOI

      10.3389/fcell.2022.949345

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Two-step regulation of centromere distribution by condensin II and the nuclear envelope proteins2022

    • Author(s)
      Sakamoto Takuya、Sakamoto Yuki、Grob Stefan、Slane Daniel、Yamashita Tomoe、Ito Nanami、Oko Yuka、Sugiyama Tomoya、Higaki Takumi、Hasezawa Seiichiro、Tanaka Maho、Matsui Akihiro、Seki Motoaki、Suzuki Takamasa、Grossniklaus Ueli、Matsunaga Sachihiro
    • Journal Title

      Nature Plants

      Volume: 8 Issue: 8 Pages: 940-953

    • DOI

      10.1038/s41477-022-01200-3

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Bone marrow imaging reveals the migration dynamics of neonatal hematopoietic stem cells2022

    • Author(s)
      Takihara Yuji、Higaki Takumi、Yokomizo Tomomasa、Umemoto Terumasa、Ariyoshi Kazunori、Hashimoto Michihiro、Sezaki Maiko、Takizawa Hitoshi、Inoue Toshihiro、Suda Toshio、Mizuno Hidenobu
    • Journal Title

      Communications Biology

      Volume: 5 Issue: 1 Pages: 776-776

    • DOI

      10.1038/s42003-022-03733-x

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules.2022

    • Author(s)
      Hotta T, McAlear TS, Yue Y, Higaki T, Haynes SE, Nesvizhskii AI, Sept D, Verhey KJ, Bechstedt S, Ohi R
    • Journal Title

      Curr Biol

      Volume: 32 Issue: 18 Pages: 3898-3910

    • DOI

      10.1016/j.cub.2022.07.027

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Machine learning and feature analysis of the cortical microtubule organization of Arabidopsis cotyledon pavement cells.2022

    • Author(s)
      Yoshida D, Akita K, Higaki T
    • Journal Title

      Protoplasma

      Volume: - Issue: 3 Pages: 987-998

    • DOI

      10.1007/s00709-022-01813-7

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disease-associated H58Y mutation affects the nuclear dynamics of human DNA topoisomerase IIβ.2022

    • Author(s)
      Morotomi-Yano K, Hiromoto Y, Higaki T, Yano KI
    • Journal Title

      Sci Rep

      Volume: 12 Issue: 1 Pages: 20627-20627

    • DOI

      10.1038/s41598-022-24883-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Calcium signaling contributes to xylem vessel cell differentiation via post-transcriptional regulation of VND7 downstream events2021

    • Author(s)
      Kamon E, Noda C, Higaki T, Demura T, Ohtani M
    • Journal Title

      Plant Biotechnology

      Volume: 38 Issue: 3 Pages: 331-337

    • DOI

      10.5511/plantbiotechnology.21.0519a

    • NAID

      130008091606

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2021-09-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The boundary-expressed <i>EPIDERMAL PATTERNING FACTOR-LIKE2</i> gene encoding a signaling peptide promotes cotyledon growth during <i>Arabidopsis thaliana</i> embryogenesis2021

    • Author(s)
      Fujihara Rina、Uchida Naoyuki、Tameshige Toshiaki、Kawamoto Nozomi、Hotokezaka Yugo、Higaki Takumi、Simon Rudiger、Torii Keiko U、Tasaka Masao、Aida Mitsuhiro
    • Journal Title

      Plant Biotechnology

      Volume: 38 Issue: 3 Pages: 317-322

    • DOI

      10.5511/plantbiotechnology.21.0508a

    • NAID

      130008091603

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2021-09-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Involvement of the Membrane Trafficking Factor PATROL1 in the Salinity Stress Tolerance of <i>Arabidopsis thaliana</i>2021

    • Author(s)
      Sato F, Iba K, Higaki T
    • Journal Title

      CYTOLOGIA

      Volume: 86 Issue: 2 Pages: 119-126

    • DOI

      10.1508/cytologia.86.119

    • NAID

      130008056667

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2021-06-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana2021

    • Author(s)
      Suzuki Reira、Yamada Mizuki、Higaki Takumi、Aida Mitsuhiro、Kubo Minoru、Tsai Allen Yi-Lun、Sawa Shinichiro
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 755610-755610

    • DOI

      10.3389/fpls.2021.755610

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Metal-nano-ink coating for monitoring and quantification of cotyledon epidermal cell morphogenesis.2021

    • Author(s)
      Kikukawa K, Yoshimura K, Watanabe A, Higaki T
    • Journal Title

      Front Plant Sci

      Volume: 12 Pages: 745980-745980

    • DOI

      10.3389/fpls.2021.745980

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The phosphorylation status of NPH3 affects photosensory adaptation during the phototropic response.2021

    • Author(s)
      Kimura T, Haga K, Nomura Y, Higaki T, Nakagami H, Sakai T
    • Journal Title

      Plant Physiol

      Volume: 187 Issue: 2 Pages: 981-995

    • DOI

      10.1093/plphys/kiab281

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamic Rearrangement and Directional Migration of Tubular Vacuoles are Required for the Asymmetric Division of the <i>Arabidopsis</i> Zygote2021

    • Author(s)
      Matsumoto Hikari、Kimata Yusuke、Higaki Takumi、Higashiyama Tetsuya、Ueda Minako
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 8 Pages: 1280-1289

    • DOI

      10.1093/pcp/pcab075

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A three-dimensional scanning system for digital archiving and quantitative evaluation of Arabidopsis plant architectures.2021

    • Author(s)
      Kunita I, Morita MT, Toda M, Higaki T
    • Journal Title

      Plant Cell Physiol

      Volume: 62 Issue: 12 Pages: 1975-1982

    • DOI

      10.1093/pcp/pcab068

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Disruption of actin filaments delays accumulation of cell plate membranes after chromosome separation.2021

    • Author(s)
      Maeda K, Higaki T
    • Journal Title

      Plant Sig Behav

      Volume: 16 Issue: 4 Pages: 1873586-1873586

    • DOI

      10.1080/15592324.2021.1873586

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Discovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules.2021

    • Author(s)
      Ishida T, Yoshimura H, Takekawa M, Higaki T, Ideue T, Hatano M, Igarashi M, Tani T, Sawa S, Ishikawa H.
    • Journal Title

      Sci Rep

      Volume: 23 Issue: 1 Pages: 6077-6077

    • DOI

      10.1038/s41598-021-85501-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Four-dimensional imaging with virtual reality to quantitatively explore jigsaw puzzle-like morphogenesis of <i>Arabidopsis</i> cotyledon pavement cells2020

    • Author(s)
      Higaki T, Mizuno H
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 4 Pages: 429-435

    • DOI

      10.5511/plantbiotechnology.20.0605a

    • NAID

      130007960223

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2020-12-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Elevated CO2 promotes satellite stomata production in young cotyledons of Arabidopsis thaliana.2020

    • Author(s)
      Higaki T, Akita K, Hasezawa S
    • Journal Title

      Genes Cells

      Volume: 25 Issue: 7 Pages: 475-482

    • DOI

      10.1111/gtc.12773

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expresion of a fungal exo-β-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactans in the regulation of cell shape.2020

    • Author(s)
      Yoshimi Y, Hara K, Yoshimura M, Tanaka N, Higaki T, Tsumuraya Y, Kotake T
    • Journal Title

      J Exp Bot

      Volume: 71 Issue: 18 Pages: 5414-5424

    • DOI

      10.1093/jxb/eraa236

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes2020

    • Author(s)
      Kimata Yusuke、Higaki Takumi、Kurihara Daisuke、Ando Naoe、Matsumoto Hikari、Higashiyama Tetsuya、Ueda Minako
    • Journal Title

      Quantitative Plant Biology

      Volume: 1

    • DOI

      10.1017/qpb.2020.4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity.2020

    • Author(s)
      Lu YJ, Li P, Shimono M, Corrion A, Higaki T, He SY, Day B
    • Journal Title

      Nat Commun

      Volume: 11 Issue: 1 Pages: 6234-6234

    • DOI

      10.1038/s41467-020-20007-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Coefficient of variation as an image-intensity metric for cytoskeleton bundling.2020

    • Author(s)
      Higaki T, Akita K, Katoh K
    • Journal Title

      Sci Rep

      Volume: 10 Issue: 1 Pages: 22187-22187

    • DOI

      10.1038/s41598-020-79136-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Noninvasive Early Detection of Calpain 2-Enriched Non-Small Cell Lung Cancer Using a Human Serum Albumin-Bounded Calpain 2 Nanosensor2020

    • Author(s)
      Kwon Seung-Hae、Wi Taemin、Park Yong Il、Kim Min Woo、Lee Gibok、Higaki Takumi、Choi Jung Hoon、Lee Ruda
    • Journal Title

      Bioconjugate Chemistry

      Volume: 31 Issue: 3 Pages: 803-812

    • DOI

      10.1021/acs.bioconjchem.9b00870

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Actin Filament Disruption Alters Phragmoplast Microtubule Dynamics during the Initial Phase of Plant Cytokinesis2020

    • Author(s)
      Maeda Keisho、Sasabe Michiko、Hanamata Shigeru、Machida Yasunori、Hasezawa Seiichiro、Higaki Takumi
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 3 Pages: 445-456

    • DOI

      10.1093/pcp/pcaa003

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Progression of Xylem Vessel Cell Differentiation is Dependent on the Activity Level of VND7 in Arabidopsis thaliana2019

    • Author(s)
      Hirai Risaku、Higaki Takumi、Takenaka Yuto、Sakamoto Yuki、Hasegawa Junko、Matsunaga Sachihiro、Demura Taku、Ohtani Misato
    • Journal Title

      Plants

      Volume: 9 Issue: 1 Pages: 39-39

    • DOI

      10.3390/plants9010039

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Basic Proline-Rich Protein-Mediated Microtubules Are Essential for Lobe Growth and Flattened Cell Geometry2019

    • Author(s)
      Wong Jeh Haur、Kato Takehide、Belteton Samuel A.、Shimizu Rie、Kinoshita Nene、Higaki Takumi、Sakumura Yuichi、Szymanski Daniel B.、Hashimoto Takashi
    • Journal Title

      Plant Physiology

      Volume: 181 Issue: 4 Pages: 1535-1551

    • DOI

      10.1104/pp.19.00811

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Theoretical models for branch formation in plants2019

    • Author(s)
      Nakamasu Akiko、Higaki Takumi
    • Journal Title

      Journal of Plant Research

      Volume: 132 Issue: 3 Pages: 325-333

    • DOI

      10.1007/s10265-019-01107-9

    • NAID

      40021897166

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of Root-Knot Nematode Behavior by Seed-Coat Mucilage-Derived Attractants.2019

    • Author(s)
      Tsai, A, Y-L., Higaki, T., Nguyen, C-N., Perfus-Barbeoch, L., Favery, B., and Sawa, S.
    • Journal Title

      Mol. Plants.

      Volume: 12 Issue: 1 Pages: 99-122

    • DOI

      10.1016/j.molp.2018.11.008

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Developmental analyses of divarications in leaves of an aquatic fern Microsorum pteropus and its varieties2019

    • Author(s)
      Miyoshi Saori、Kimura Seisuke、Ootsuki Ryo、Higaki Takumi、Nakamasu Akiko
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 1 Pages: e0210141-e0210141

    • DOI

      10.1371/journal.pone.0210141

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Polar vacuolar distribution is essential for accurate asymmetric division ofArabidopsiszygotes2019

    • Author(s)
      Kimata Yusuke、Kato Takehide、Higaki Takumi、Kurihara Daisuke、Yamada Tomomi、Segami Shoji、Morita Miyo Terao、Maeshima Masayoshi、Hasezawa Seiichiro、Higashiyama Tetsuya、Tasaka Masao、Ueda Minako
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 116 Issue: 6 Pages: 2338-2343

    • DOI

      10.1073/pnas.1814160116

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An induction system for clustered stomata by sugar solution immersion treatment in Arabidopsis thaliana seedlings2019

    • Author(s)
      Akita K, Higaki T
    • Journal Title

      Journal of Visualized Experiments

      Volume: 144 Issue: 144

    • DOI

      10.3791/58951

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulators involved in responses to volatile organic compounds in plants2019

    • Author(s)
      Nagashima A, Higaki T, Koeduka T, Ishigami K, Hosokawa S, Watanabe H, Matsui K, Hasezawa S, Touhara K
    • Journal Title

      Journal of Biological Chemistry

      Volume: 294 Issue: 7 Pages: 2256-2266

    • DOI

      10.1074/jbc.ra118.005843

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin2019

    • Author(s)
      Minami Tanaka, Yuki Fujii, Kazumi Hirano, Takumi Higaki, Akira Nagasaki, Ryoki Ishikawa, Takaharu Okajima, Kaoru Katoh
    • Journal Title

      Genes to Cells

      Volume: 24 Issue: 3 Pages: 202-213

    • DOI

      10.1111/gtc.12671

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reactive Oxygen Species Production and Stimulated Endocytosis in Tobacco BY-2 Cells Treated with <i>Erwinia carotovora</i> Culture Filtrate2018

    • Author(s)
      Hirakawa Y, Hasezawa S, Higaki T
    • Journal Title

      CYTOLOGIA

      Volume: 83 Issue: 3 Pages: 289-293

    • DOI

      10.1508/cytologia.83.289

    • NAID

      130007497289

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2018-09-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cortical microtubules and fusicoccin response in clustered stomatal guard cells induced by sucrose solutionimmersion.2018

    • Author(s)
      Akita K, Hasezawa S, Higaki T
    • Journal Title

      Plant Signaling and Behavior

      Volume: 13 Issue: 4 Pages: 1454815-1454815

    • DOI

      10.1080/15592324.2018.1454815

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Actin reorganization triggers rapid cell elongation in roots2018

    • Author(s)
      Takatsuka Hirotomo、Higaki Takumi、Umeda Masaaki
    • Journal Title

      Plant Physiology

      Volume: 178 Issue: 3 Pages: 1130-1141

    • DOI

      10.1104/pp.18.00557

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis.2018

    • Author(s)
      Hirano T, Konno H, Takeda S, Dolan L, Kato M, Aoyama T, Higaki T, Takigawa-Imamura H, Sato MH.
    • Journal Title

      Nat Plants.

      Volume: 4 Issue: 11 Pages: 888-897

    • DOI

      10.1038/s41477-018-0277-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 画像解析に基づく細胞の定量評価と認識2022

    • Author(s)
      檜垣 匠
    • Organizer
      日本農薬学会第48回大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Four-dimensional digital archiving and quantitative evaluation of Arabidopsis architectures.2022

    • Author(s)
      Takumi Higaki
    • Organizer
      International symposium on “Plant-Structure-Optimization”
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物細胞生物学を起点とした画像の定量評価と機械学習2021

    • Author(s)
      檜垣 匠
    • Organizer
      第30回日本バイオイメージング学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Three-dimensional reconstruction of Arabidopsis plant architecture.2021

    • Author(s)
      Takumi Higaki
    • Organizer
      Special Satellite Event “Plant Structure Optimization” in the 7th International Conference of Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 画像解析技術の援用による植物細胞骨格研究2021

    • Author(s)
      檜垣匠
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 微小管結合タンパク質RIC1 過剰発現によ る子葉器官および表皮細胞の形態変化2021

    • Author(s)
      菊川琴美、檜垣匠
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナ子葉敷石細胞における微小 管構造特徴と細胞形態の関係2021

    • Author(s)
      吉田大一、Liu Bo、檜垣匠
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 重力感受細胞におけるLZY タンパク質のライブイメージング2021

    • Author(s)
      森祥伍,中村守貴,押田龍一郎,四方明格,西村岳志,古谷将彦,桧垣匠,森田(寺尾)美代
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 視体積交差法による植物形態の立体再構成と計測2020

    • Author(s)
      檜垣匠
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 生物画像の定量・分類・認識2020

    • Author(s)
      檜垣匠
    • Organizer
      第67回日本生態学会大会シンポジウム「植物フェノタイピング:表現型データの定量化,モデル化とその自動化をもう一度考える」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Plasma membrane delivery of H+-ATPse for stomatal response.2019

    • Author(s)
      Higaki Takumi
    • Organizer
      International Symposium 'Mechanisms of plant perception: from the endogenous to the exogenous'
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 電子顕微鏡画像解析による生物微細構造の検出と認識2019

    • Author(s)
      檜垣匠
    • Organizer
      公益社団法人日本顕微鏡学会第62回シンポジウム「AIを用いた顕微イメージングの将来~科学技術から物理化学,そして生命科学への貢献~」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Three-dimensional imaging and morphometry of large vacuole regeneration in plant cultured cells.2019

    • Author(s)
      Higaki Takumi
    • Organizer
      Workshop in the 42nd Annual Meeting of the Molecular Biology Society of Japan “The vacuole/lysosome; Where does it come from, how is it maintained and what does it do in the cell?”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ジグソーパズル型葉表皮細胞の力学的最適化戦略を探る2019

    • Author(s)
      檜垣匠
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 植物細胞の形態形成機構の理解に向けたイメージングと画像解析2018

    • Author(s)
      檜垣匠
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Bioimage clustering and classification2018

    • Author(s)
      Takumi Higaki
    • Organizer
      KAIST-KU Joint Symposium 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 熊本大学ニュース「細胞の骨組みの特徴に基づいたAIによる細胞の自動分類と分析」

    • URL

      https://www.kumamoto-u.ac.jp/whatsnew/sizen/20221020

    • Related Report
      2022 Annual Research Report
  • [Remarks] Mapping 4D cell models with VR

    • URL

      https://www.leica-microsystems.com/science-lab/mapping-4d-cell-models-with-vr/

    • Related Report
      2021 Annual Research Report
  • [Remarks] AIを活用した植物細胞形状の追跡計測技術の開発

    • URL

      https://www.kumamoto-u.ac.jp/whatsnew/sizen/20210921

    • Related Report
      2021 Annual Research Report
  • [Remarks] 熊本大学ニュース: 熊本大学『フィロソフィアの扉』第31回「国際先端科学技術研究機構」

    • URL

      https://www.youtube.com/watch?v=4Nr4uK-WAH4

    • Related Report
      2020 Annual Research Report
  • [Remarks] 細胞の骨組みの状態を高感度に測る技術を開発

    • URL

      https://www.kumamoto-u.ac.jp/whatsnew/sizen/20201221

    • Related Report
      2020 Annual Research Report
  • [Remarks] EurekAlert! Science News Releases

    • URL

      https://www.eurekalert.org/pub_releases/2021-01/ku-ahs011421.php

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-07-20   Modified: 2024-01-30  

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