Decryption of the ubiquitin code by chemo-technologies and advanced proteomics

• Project Area: New frontier for ubiquitin biology driven by chemo-technologies
• Project/Area Number: 18H05498
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: SAEKI Yasushi 公益財団法人東京都医学総合研究所, 基礎医科学研究分野, プロジェクトリーダー (80462779)
• Co-Investigator(Kenkyū-buntansha): 大竹 史明 星薬科大学, 先端生命科学研究所, 特任准教授 (60447373)
• Project Period (FY): 2018-06-29 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥131,430,000 (Direct Cost: ¥101,100,000、Indirect Cost: ¥30,330,000); Fiscal Year 2022: ¥28,860,000 (Direct Cost: ¥22,200,000、Indirect Cost: ¥6,660,000); Fiscal Year 2021: ¥28,860,000 (Direct Cost: ¥22,200,000、Indirect Cost: ¥6,660,000); Fiscal Year 2020: ¥28,860,000 (Direct Cost: ¥22,200,000、Indirect Cost: ¥6,660,000); Fiscal Year 2019: ¥28,860,000 (Direct Cost: ¥22,200,000、Indirect Cost: ¥6,660,000); Fiscal Year 2018: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
• Keywords: ユビキチン / プロテアソーム / 質量分析 / 標的タンパク質分解誘導剤 / 液－液相分離 / p97 / ケモテクノロジー / 質量分析計

Outline of Final Research Achievements

In this planned research, we have developed several advanced proteomics methods to analyze the ubiquitin system and strongly promoted this research project area. Major achievements include the elucidation of the ubiquitin decoders for proteasomal degradation, the discovery of the liquid-liquid phase separation of the proteasome, the development of monobodies that inhibit the ubiquitin decoder, and the discovery of new architectures of branched ubiquitin chains induced by PROTAC and its formation mechanisms. We believe that these findings will contribute to the future development of ubiquitin research and drug discovery.

Academic Significance and Societal Importance of the Research Achievements

ユビキチン・プロテアソーム系を駆動するユビキチンコードとその解読分子を解明した本研究成果は、基礎科学の発展のみならず、がんや神経変性疾患など本分解経路が関与する様々な疾患の発症機構解明や近年爆発的に拡大しているユビキチン創薬の発展に大きく貢献する。

Research Products

• [Int'l Joint Research] Max Planck Institute of Biochemistry(ドイツ)
• [Journal Article] An attenuated vaccinia vaccine encoding the severe acute respiratory syndrome coronavirus-2 spike protein elicits broad and durable immune responses, and protects cynomolgus macaques and human angiotensin-converting enzyme 2 transgenic mice from severe acute respiratory syndrome coronavirus-2 and its variants. (2022)
  • Author(s): Ishigaki H, Yasui F, Nakayama M, Endo A, Yamamoto N, Yamaji K, Nguyen CT, Kitagawa Y, Sanada T, Honda T, Munakata T, Higa M, Toyama S, Kono R, Takagi A, Matsumoto Y, Koseki A, Hayashi K, Shiohara M, Ishii K, Saeki Y, Itoh Y, Kohara M.
  • Journal Title: Front Microbiol. Volume: 13 Pages: 967019-967019
  • DOI: 10.3389/fmicb.2022.967019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] cIAP1-based degraders induce degradation via branched ubiquitin architectures (2022)
  • Author(s): Akizuki Yoshino、Morita Mai、Mori Yuki、Kaiho-Soma Ai、Dixit Shivani、Endo Akinori、Shimogawa Marie、Hayashi Gosuke、Naito Mikihiko、Okamoto Akimitsu、Tanaka Keiji、Saeki Yasushi、Ohtake Fumiaki
  • Journal Title: Nature Chemical Biology Volume: 19 Issue: 3 Pages: 311-322
  • DOI: 10.1038/s41589-022-01178-1
  • Peer Reviewed
• [Journal Article] Branched ubiquitin code: from basic biology to targeted protein degradation (2022)
  • Author(s): Ohtake Fumiaki
  • Journal Title: The Journal of Biochemistry Volume: 171 Issue: 4 Pages: 361-366
  • DOI: 10.1093/jb/mvac002
  • Peer Reviewed
• [Journal Article] Quality Control: Maintaining molecular order and preventing cellular chaos (2022)
  • Author(s): Neal Sonya、Ohtake Fumiaki、Cuervo Ana Maria、Hegde Ramanujan S.、Jakob Ursula、Lazarou Michael、Gilbert Wendy V.、Chen Zhijian J.、Tooze Sharon A.、Haber James E.、Walters Kylie J.、Hartl F. Ulrich
  • Journal Title: Molecular Cell Volume: 82 Issue: 8 Pages: 1390-1397
  • DOI: 10.1016/j.molcel.2022.04.002
  • Peer Reviewed
• [Journal Article] ENTREP/FAM189A2 encodes a new ITCH ubiquitin ligase activator that is downregulated in breast cancer (2022)
  • Author(s): Takumi Tsunoda, Miho Riku, Norika Yamada, Hikaru Tsuchiya, Takuya Tomita, Minako Suzuki, Mari Kizuki, Akihito Inoko, Hideaki Ito, Kenta Murotani, Hideki Murakami, Yasushi Saeki, Kenji Kasai
  • Journal Title: EMBO reports Volume: 23 Issue: 2
  • DOI: 10.15252/embr.202051182
  • Peer Reviewed / Open Access
• [Journal Article] The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis (2022)
  • Author(s): Yoko Hasegawa, Thais Huarancca Reyes, Tomohiro Uemura, Anirban Baral, Akari Fujimaki, Yongming Luo, Yoshie Morita, Yasushi Saeki, Shugo Maekawa, Shigetaka Yasuda, Koki Mukuta, Yoichiro Fukao, Keiji Tanaka, Akihiko Nakano, Junpei Takagi, Rishikesh P Bhalerao, Junji Yamaguchi, Takeo Sato
  • Journal Title: THE PLANT CELL Volume: 34 Issue: 4 Pages: 1354-1374
  • DOI: 10.1093/plcell/koac014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Frontier of the ubiquitin code research (2021)
  • Author(s): 佐伯 泰、遠藤 彬則、大竹 史明
  • Journal Title: MEDCHEM NEWS Volume: 31 Issue: 3 Pages: 136-142
  • DOI: 10.14894/medchem.31.3_136
  • NAID: 130008070855
  • ISSN: 2432-8618, 2432-8626
  • Year and Date: 2021-08-01
  • Peer Reviewed
• [Journal Article] RNA polymerase II condensate formation and association with Cajal and histone locus bodies in living human cells (2021)
  • Author(s): Imada Takashi、Shimi Takeshi、Kaiho Ai、Saeki Yasushi、Kimura Hiroshi
  • Journal Title: Genes to Cells Volume: - Issue: 5 Pages: 298-312
  • DOI: 10.1111/gtc.12840
  • Peer Reviewed / Open Access
• [Journal Article] Discovery of a Highly Potent and Selective Degrader Targeting Hematopoietic Prostaglandin D Synthase via In Silico Design (2021)
  • Author(s): Yokoo Hidetomo、Shibata Norihito、Endo Akinori、Ito Takahito、Yanase Yuta、Murakami Yuki、Fujii Kiyonaga、Hamamura Kengo、Saeki Yasushi、Naito Mikihiko、Aritake Kosuke、Demizu Yosuke
  • Journal Title: Journal of Medicinal Chemistry Volume: 64 Issue: 21 Pages: 15868-15882
  • DOI: 10.1021/acs.jmedchem.1c01206
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Design, Synthesis, and Evaluation of Trivalent PROTACs Having a Functionalization Site with Controlled Orientation (2021)
  • Author(s): Huang Yifan、Yokoe Hiromasa、Kaiho-Soma Ai、Takahashi Kazunori、Hirasawa Yusuke、Morita Hiroshi、Ohtake Fumiaki、Kanoh Naoki
  • Journal Title: Bioconjugate Chemistry Volume: 33 Issue: 1 Pages: 142-151
  • DOI: 10.1021/acs.bioconjchem.1c00490
  • Peer Reviewed
• [Journal Article] MIND bomb 2 prevents RIPK1 kinase activity-dependent and -independent apoptosis through ubiquitylation of cFLIPL (2021)
  • Author(s): Nakabayashi Osamu、Takahashi Hirotaka、Moriwaki Kenta、Komazawa-Sakon Sachiko、Ohtake Fumiaki、Murai Shin、Tsuchiya Yuichi、Koyahara Yuki、Saeki Yasushi、Yoshida Yukiko、Yamazaki Soh、Tokunaga Fuminori、Sawasaki Tatsuya、Nakano Hiroyasu
  • Journal Title: Communications Biology Volume: 4 Issue: 1 Pages: 80-80
  • DOI: 10.1038/s42003-020-01603-y
  • Peer Reviewed / Open Access
• [Journal Article] TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains (2021)
  • Author(s): Kaiho-Soma Ai、Akizuki Yoshino、Igarashi Katsuhide、Endo Akinori、Shoda Takuji、Kawase Yasuko、Demizu Yosuke、Naito Mikihiko、Saeki Yasushi、Tanaka Keiji、Ohtake Fumiaki
  • Journal Title: Molecular Cell Volume: 81 Issue: 7 Pages: 1411-1424.e7
  • DOI: 10.1016/j.molcel.2021.01.023
  • Peer Reviewed / Open Access
• [Journal Article] Proteasome phase separation for degradation (2020)
  • Author(s): 佐伯 泰、安田 さや香、土屋 光
  • Journal Title: 生化学 Volume: 92 Issue: 6 Pages: 822-826
  • DOI: 10.14952/SEIKAGAKU.2020.920822
  • NAID: 40022454281
  • ISSN: 0037-1017
  • Year and Date: 2020-12-25
  • Peer Reviewed
• [Journal Article] A novel method to determine the chain length of the polyubiquitinated proteins (2020)
  • Author(s): 土屋 光、佐伯 泰
  • Journal Title: 生化学 Volume: 92 Issue: 1 Pages: 14-19
  • DOI: 10.14952/SEIKAGAKU.2020.920014
  • ISSN: 0037-1017
  • Year and Date: 2020-02-25
  • Peer Reviewed / Open Access
• [Journal Article] Branched ubiquitin chains and acetylated ubiquitin (2020)
  • Author(s): 大竹 史明
  • Journal Title: 生化学 Volume: 92 Issue: 1 Pages: 57-63
  • DOI: 10.14952/SEIKAGAKU.2020.920057
  • ISSN: 0037-1017
  • Year and Date: 2020-02-25
  • Peer Reviewed
• [Journal Article] Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses. (2020)
  • Author(s): Oikawa D, Sato Y, Ohtake F, Komakura K, Hanada K, Sugawara K, Terawaki S, Mizukami Y, Phuong HT, Iio K, Obika S, Fukushi M, Irie T, Tsuruta D, Sakamoto S, Tanaka K, Saeki Y, Fukai S, Tokunaga F.
  • Journal Title: Commun. Biol. Volume: 3 Issue: 1 Pages: 163-163
  • DOI: 10.1038/s42003-020-0882-8
  • Peer Reviewed / Open Access
• [Journal Article] The HOIL-1L ligase modulates immune signalling and cell death via monoubiquitination of LUBAC (2020)
  • Author(s): Fuseya Yasuhiro、Fujita Hiroaki、Kim Minsoo、Ohtake Fumiaki、Nishide Akira、Sasaki Katsuhiro、Saeki Yasushi、Tanaka Keiji、Takahashi Ryosuke、Iwai Kazuhiro
  • Journal Title: Nature Cell Biology Volume: 22 Issue: 6 Pages: 663-673
  • DOI: 10.1038/s41556-020-0517-9
  • Peer Reviewed / Open Access
• [Journal Article] α-synuclein strains that cause distinct pathologies differentially inhibit proteasome (2020)
  • Author(s): Suzuki Genjiro、Imura Sei、Hosokawa Masato、Katsumata Ryu、Nonaka Takashi、Hisanaga Shin-Ichi、Saeki Yasushi、Hasegawa Masato
  • Journal Title: eLife Volume: 9
  • DOI: 10.7554/elife.56825
  • Peer Reviewed / Open Access
• [Journal Article] A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets (2020)
  • Author(s): Watanabe Masashi、Saeki Yasushi、Takahashi Hidehisa、Ohtake Fumiaki、Yoshida Yukiko、Kasuga Yusuke、Kondo Takeshi、Yaguchi Hiroaki、Suzuki Masanobu、Ishida Hiroki、Tanaka Keiji、Hatakeyama Shigetsugu
  • Journal Title: Communications Biology Volume: 3 Issue: 1 Pages: 592-592
  • DOI: 10.1038/s42003-020-01328-y
  • Peer Reviewed / Open Access
• [Journal Article] Ribosomal protein S7 ubiquitination during ER stress in yeast is associated with selective mRNA translation and stress outcome (2020)
  • Author(s): Matsuki Yasuko、Matsuo Yoshitaka、Nakano Yu、Iwasaki Shintaro、Yoko Hideyuki、Udagawa Tsuyoshi、Li Sihan、Saeki Yasushi、Yoshihisa Tohru、Tanaka Keiji、Ingolia Nicholas T.、Inada Toshifumi
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 19669-19669
  • DOI: 10.1038/s41598-020-76239-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multi-Step Ubiquitin Decoding Mechanism for Proteasomal Degradation (2020)
  • Author(s): Tsuchiya Hikaru、Endo Akinori、Saeki Yasushi
  • Journal Title: Pharmaceuticals Volume: 13 Issue: 6 Pages: 128-128
  • DOI: 10.3390/ph13060128
  • Peer Reviewed / Open Access
• [Journal Article] Mass Spectrometry Technologies for Deciphering the Ubiquitin Code (2020)
  • Author(s): Ohtake Fumiaki
  • Journal Title: Trends in Biochemical Sciences Volume: 45 Issue: 9 Pages: 820-821
  • DOI: 10.1016/j.tibs.2020.04.008
  • Peer Reviewed
• [Journal Article] Stress- and ubiquitylation-dependent phase separation of the proteasome (2020)
  • Author(s): Yasuda Sayaka、Tsuchiya Hikaru、Kaiho Ai、Guo Qiang、Ikeuchi Ken、Endo Akinori、Arai Naoko、Ohtake Fumiaki、Murata Shigeo、Inada Toshifumi、Baumeister Wolfgang、Fernandez-Busnadiego Ruben、Tanaka Keiji、Saeki Yasushi
  • Journal Title: Nature Volume: 578 Issue: 7794 Pages: 296-300
  • DOI: 10.1038/s41586-020-1982-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation (2020)
  • Author(s): A. Nishiyama, C. Mulholland, S. Bultmann, S. Kori, A. Endo, Y. Saeki, W. Qin, C. Trummer, Y. Chiba, H. Yokoyama, S. Kumamoto, T. Kawakami, H. Hojo, G. Nagae, H. Aburatani, K. Tanaka, K. Arita, H. Leonhardt, M. Nakanishi
  • Journal Title: Nat. Commun. Volume: 11 Issue: 1 Pages: 1222-1222
  • DOI: 10.1038/s41467-020-15006-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] RQT complex dissociates ribosomes collided on endogenous RQC substrate SDD1 (2020)
  • Author(s): Matsuo Yoshitaka、Tesina Petr、Nakajima Shizuka、Mizuno Masato、Endo Akinori、Buschauer Robert、Cheng Jingdong、Shounai Okuto、Ikeuchi Ken、Saeki Yasushi、Becker Thomas、Beckmann Roland、Inada Toshifumi
  • Journal Title: Nature Structural & Molecular Biology Volume: 27 Issue: 4 Pages: 323-332
  • DOI: 10.1038/s41594-020-0393-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 最先端プロテオミクス解析を用いたユビキチン研究 (2020)
  • Author(s): 佐伯 泰
  • Journal Title: Farumashia Volume: 56 Issue: 1 Pages: 21-25
  • DOI: 10.14894/faruawpsj.56.1_21
  • NAID: 130007779041
  • ISSN: 0014-8601, 2189-7026
  • Peer Reviewed / Open Access
• [Journal Article] Stepwise multipolyubiquitination of p53 by the E6AP-E6 ubiquitin ligase complex (2019)
  • Author(s): Masuda Y, Saeki Y, Arai N, Kawai H, Kukimoto I, Tanaka K, Masutani C.
  • Journal Title: J Biol Chem Volume: 294 Issue: 41 Pages: 14860-14875
  • DOI: 10.1074/jbc.ra119.008374
  • Peer Reviewed / Open Access
• [Journal Article] Structural insights into ubiquitin recognition and Ufd1 interaction of Npl4 (2019)
  • Author(s): Sato Yusuke、Tsuchiya Hikaru、Yamagata Atsushi、Okatsu Kei、Tanaka Keiji、Saeki Yasushi、Fukai Shuya
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 5708-5708
  • DOI: 10.1038/s41467-019-13697-y
  • Peer Reviewed / Open Access
• [Journal Article] Collided ribosomes form a unique structural interface to induce Hel2‐driven quality control pathways (2019)
  • Author(s): Ikeuchi Ken、Tesina Petr、Matsuo Yoshitaka、Sugiyama Takato、Cheng Jingdong、Saeki Yasushi、Tanaka Keiji、Becker Thomas、Beckmann Roland、Inada Toshifumi
  • Journal Title: The EMBO Journal Volume: 38 Issue: 5
  • DOI: 10.15252/embj.2018100276
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Methods to measure ubiquitin chain length and linkage. (2019)
  • Author(s): Ohtake, F., Tsuchiya, H., Tanaka, K., and Saeki, Y.
  • Journal Title: Methods Enzymol Volume: 618 Pages: 105-133
  • DOI: 10.1016/bs.mie.2018.12.019
  • Peer Reviewed / Open Access
• [Journal Article] Deubiquitylases USP5 and USP13 are recruited to and regulate heat-induced stress granules through their deubiquitylating activities (2018)
  • Author(s): Xie Xuan、Matsumoto Shunsuke、Endo Akinori、Fukushima Toshiaki、Kawahara Hiroyuki、Saeki Yasushi、Komada Masayuki
  • Journal Title: Journal of Cell Science Volume: 131 Issue: 8 Pages: 210856-210856
  • DOI: 10.1242/jcs.210856
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] HERC2 facilitates BLM and WRN helicase complex interaction with RPA to suppress G-quadruplex DNA. (2018)
  • Author(s): Wu W, Rokutanda N, Takeuchi J, Lai Y, Maruyama R, Togashi Y, Nishikawa H, Arai N, Miyoshi Y,Suzuki N, Saeki Y, Tanaka K, Ohta T.
  • Journal Title: Cancer Res. Volume: 78 Issue: 22 Pages: 6371-6385
  • DOI: 10.1158/0008-5472.can-18-1877
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Specific modification of aged proteasomes revealed by tag-exchangeable knock-in mice. (2018)
  • Author(s): Tomita, T., Hirayama, S., Sakurai, Y., Ohte, Y., Yoshihara, H., Saeki, Y., Hamazaki, J., and Murata, S.
  • Journal Title: Mol. Cell Biol. Volume: 39 Issue: 1
  • DOI: 10.1128/mcb.00426-18
  • Peer Reviewed / Open Access
• [Presentation] ケモテクノロジーが拓くユビキチンニューフロンティア (2022)
  • Author(s): 佐伯 泰
  • Organizer: 日本ケミカルバイオロジー学会第16回年会
  • Invited
• [Presentation] プロテアソーム基質の選別機構 (2022)
  • Author(s): 佐伯 泰、土屋光、林剛介、遠藤彬則
  • Organizer: 第74回日本細胞生物学会大会
  • Invited
• [Presentation] ユビキチンコード研究の最前線～基本原理の理解と創薬～ (2022)
  • Author(s): 佐伯 泰
  • Organizer: ゲノム創薬・創発フォーラム第10回シンポジウム「標的タンパク質分解誘導薬の可能性を探る」
  • Invited
• [Presentation] ケモユビキチン：生物学と合成化学の融合がもたらす新発想の創薬の芽 (2022)
  • Author(s): 古畑隆史、佐伯泰、岡本晃光
  • Organizer: 第1回日本科学振興協会（JAAS）キックオフミーティング
• [Presentation] 最先端プロテオミクスによって見えてきたプロテアソーム経路の基質選別メカニズム (2022)
  • Author(s): 佐伯 泰、土屋光、遠藤彬則
  • Organizer: 日本プロテオーム学会2022年大会
  • Invited
• [Presentation] Ubiquitylation-dependent phase separation of the proteasome (2022)
  • Author(s): Yasushi Saeki
  • Organizer: XI-ZOMES
  • Int'l Joint Research / Invited
• [Presentation] 頑張って若さを保とう！老化とタンパク質分解 (2022)
  • Author(s): 佐伯 泰
  • Organizer: 第12回CSJ化学フェスタ2022 シンポジウム「豊かな超高齢社会をつくろう～ヒトの命を支える化学～」
  • Invited
• [Presentation] 最先端プロテオミクスによって見えてきたプロテアソーム経路の基質選別メカニズム (2022)
  • Author(s): 佐伯 泰
  • Organizer: 第95回日本生化学会大会シンポジウム「ユビキチン・プロテアソーム研究のニューフロンティア」
  • Invited
• [Presentation] Ubiquitylation-dependent phase separation of the proteasome (2022)
  • Author(s): Yasushi Saeki
  • Organizer: Ubiquitin New Frontier "from Neo-Biology to Targeted Protein Degradation"
  • Int'l Joint Research / Invited
• [Presentation] 分岐型ユビキチン鎖は標的タンパク質分解誘導剤の作用を促進する (2022)
  • Author(s): 大竹史明、秋月慶乃
  • Organizer: 日本ケミカルバイオロジー学会第16回年会
  • Invited
• [Presentation] 標的タンパク質分解誘導剤によるネオユビキチンコード形成機構 (2022)
  • Author(s): 大竹史明
  • Organizer: 第95回日本生化学会大会シンポジウム「ユビキチン・プロテアソーム研究のニューフロンティア」
  • Invited
• [Presentation] Role of the branched ubiquitin chains in targeted protein degradation (2022)
  • Author(s): Fumiaki Ohtake
  • Organizer: Ubiquitin New Frontier "from Neo-Biology to Targeted Protein Degradation"
  • Int'l Joint Research / Invited
• [Presentation] プロテアソーム液滴基質のプロテオーム解析 (2021)
  • Author(s): 土屋 光、佐伯 泰
  • Organizer: 日本プロテオーム学会2021年大会
  • Invited
• [Presentation] ユビキチン依存的なプロテアソームの液-液相分離 (2021)
  • Author(s): 佐伯 泰、土屋 光、遠藤彬則
  • Organizer: 第94回日本生化学大会
  • Invited
• [Presentation] 細胞内ATPレベル低下によるユビキチン・プロテアソーム液滴の形成機構 (2021)
  • Author(s): 遠藤彬則、田中啓二、佐伯 泰
  • Organizer: 第94回日本生化学大会
  • Invited
• [Presentation] Understanding the ubiquitin-proteasome system by advanced proteomics (2021)
  • Author(s): Yasushi Saeki、Hikaru Tsuchiya、Takuya Tomita、Akinori Endo
  • Organizer: 第44回日本分子生物学会年会
  • Invited
• [Presentation] 化合物による標的タンパク質分解を誘導するユビキチンコード (2021)
  • Author(s): 大竹史明
  • Organizer: 第94回日本生化学大会
  • Invited
• [Presentation] 標的タンパク質分解薬におけるユビキチン分岐鎖と役割 (2021)
  • Author(s): 大竹史明
  • Organizer: 第40回日本認知症学会学術集会
  • Invited
• [Presentation] Branched ubiquitin chains promote chemically-induced targeted protein degradation (2021)
  • Author(s): 大竹史明、相馬愛、秋月慶乃
  • Organizer: 第44回日本分子生物学会年会
  • Invited
• [Presentation] プロテオミクス解析から明らかになるユビキチン修飾のバイオロジー (2020)
  • Author(s): 大竹史明
  • Organizer: 日本質量分析学会第68回質量分析総合討論会
  • Invited
• [Presentation] Role of ubiquitin chain architecture revealed by chemo-technology (2020)
  • Author(s): Fumiaki Ohtake
  • Organizer: 第93回日本生化学会シンポジウム
  • Invited
• [Presentation] RAD23B drives polyubiquitin chain-dependent phase separation of the proteasome (2020)
  • Author(s): Akinori Endo、Hikaru Tsuchiya、Keiji Tanaka, Yasushi Saeki
  • Organizer: 第43回日本分子生物学会年会
  • Invited
• [Presentation] ユビキチン研究の新展開：ケミカルバイオロジーとの融合から相分離まで (2020)
  • Author(s): 佐伯 泰
  • Organizer: 日本化学会第100春季年会
  • Invited
• [Presentation] ケモテクノロジーと質量分析計を活用したユビキチンコードの解読 (2019)
  • Author(s): 佐伯 泰、遠藤彬則、土屋 光、大竹史明
  • Organizer: 日本ケミカルバイオロジー学会 第14回年会
  • Invited
• [Presentation] ユビキチン研究の新展開―相分離から分解誘導剤まで (2019)
  • Author(s): 佐伯 泰
  • Organizer: 第19回 日本蛋白質科学会年会・第71回日本細胞生物学会大会 合同年次大会
  • Invited
• [Presentation] Orbitrap Fusion Lumosを使った革新的ユビキチン研究 (2019)
  • Author(s): 佐伯 泰
  • Organizer: サーモフィッシャーサイエンティフィック 質量分析フォーラム2019
  • Invited
• [Presentation] ユビキチンによるタンパク質分解のしくみ：We will degrade you! (2019)
  • Author(s): 佐伯 泰
  • Organizer: 第9回CSJ化学フェスタ
  • Invited
• [Presentation] MSを用いたユビキチンシグナルの網羅的解析 (2019)
  • Author(s): 佐伯 泰
  • Organizer: 第11回 LC/MSワークショップ
  • Invited
• [Presentation] 高浸透圧ストレスによるプロテアソーム液滴形成と核内タンパク質分解 (2019)
  • Author(s): 佐伯 泰
  • Organizer: 第14回日本臨床ストレス応答学会
  • Invited
• [Presentation] Stress- and ubiquitylation-dependent phase separation of the proteasome (2019)
  • Author(s): Yasushi Saeki
  • Organizer: 42nd MBSJ, Symposium ”Frontiers of in-cell protein sciences”
  • Int'l Joint Research / Invited
• [Presentation] 質量分析技術とケミカルツールでユビキチン鎖の複雑性に挑む (2019)
  • Author(s): 大竹史明
  • Organizer: 第4回先端ケミカルバイオロジー研究会
  • Invited
• [Presentation] Ub-ProT reveals global length and composition of protein ubiquitylation in cells. (2018)
  • Author(s): Yasushi Saeki
  • Organizer: The 8th Proteasome and Autophagy Congress
  • Int'l Joint Research
• [Presentation] The Cdc48-Rad23/Dsk2 Axis Contributes to K48 Ubiquitin Chain Specificity of the Proteasome. (2018)
  • Author(s): Yasushi Saeki
  • Organizer: FASEB SRC “Ubiquitin and Cellular Regulation”
  • Int'l Joint Research / Invited
• [Presentation] Ubiquitin-dependent phase separation for proteasomal degradation. (2018)
  • Author(s): Yasushi Saeki
  • Organizer: International Symposium on “Proteins; from the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] The NEDD4 family ubiquitin ligase ITCH regulates proteasomal degradation by seeding branched ubiquitin chains. (2018)
  • Author(s): Fumiaki Ohtake, Yasushi Saeki, and Keiji Tanaka
  • Organizer: FASEB Conference ”Ubiquitin and cellular regulation”
  • Int'l Joint Research
• [Presentation] Post-translational modification code of ubiquitin in signal transduction and protein degradation (2018)
  • Author(s): Fumiaki Ohtake, Yasushi Saeki, and Keiji Tanaka
  • Organizer: 第91回日本生化学会シンポジウム
  • Invited
• [Presentation] The ubiquitin ligases ITCH and UBR5 regulate proteasomal degradation through K48/K63 branched ubiquitin chains. (2018)
  • Author(s): Ohtake, F., Saeki, Y., and Tanaka, K.
  • Organizer: 第41回日本分子生物学会シンポジウム
  • Invited
• [Book] ユビキチン修飾と相分離 (2021)
  • Author(s): 佐伯 泰
  • Total Pages: 8
  • Publisher: 羊土社
  • ISBN: 9784758103954
• [Book] イメージング時代の構造生命科学 (2020)
  • Author(s): 安田さや香、田中啓二、佐伯 泰
  • Total Pages: 6
  • Publisher: 羊土社
  • ISBN: 9784758103855
• [Remarks] 公益財団法人東京都医学総合研究所蛋白質代謝プロジェクトホームページ
  • URL: http://www.igakuken.or.jp/pro-meta/
• [Remarks] 東京大学大学院新領域創成科学研究科メディカル情報生命専攻ホームページ
  • URL: https://www.cbms.k.u-tokyo.ac.jp/lab/saeki.html
• [Remarks] 星薬科大学先端生命科学研究所蛋白質分解創薬研究室ホームページ
  • URL: https://jupiter.hoshi.ac.jp/lab/bunkai/
• [Remarks] プレスリリース「がんの治療標的タンパク質の分解を担う酵素を発見」
  • URL: https://www.hoshi.ac.jp/wp-content/uploads/2023/04/b6e1c422d7e91ef47e39955bd1cfe478.pdf
• [Remarks] 新学術領域研究「ケモテクノロジーが拓くユビキチンニューフロンティア」ホームページ
  • URL: http://ubiquitin.jp/index.html
• [Remarks] 東京大学大学院新領域創成科学研究科メディカル情報生命専攻ホームページ
  • URL: http://www.cbms.k.u-tokyo.ac.jp/lab/rinsho.html#saeki
• [Remarks] 公益財団法人東京都医学総合研究所蛋白質代謝研究室ホームページ
  • URL: http://www.igakuken.or.jp/pro-meta/
• [Remarks] プレスリリース「標的タンパク質分解誘導剤の効果を促進する酵素を発見」
  • URL: https://www.hoshi.ac.jp/site/information/wp-content/uploads/2021/02/802f10a59ff7ef0a252fdb8c13a53c51.pdf
• [Remarks] 液－液相分離が担う核内タンパク質分解機構の発見
  • URL: http://www.igakuken.or.jp/topics/2020/0205.html
• [Remarks] Proteasome Phase separation for destruction
  • URL: http://www.igakuken.or.jp/english/topics/2020/e-0206.html
• [Remarks] 不要なタンパク質が分解を受ける前に解きほぐされる仕組み
  • URL: http://www.igakuken.or.jp/topics/2019/1213.html
• [Patent(Industrial Property Rights)] ユビキチンに対する人工抗体 (2023)
  • Inventor(s): 佐伯泰（他7名）
  • Industrial Property Rights Holder: 東京都医学総合研究所、東海国立大学機構、京都大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2023
  • Overseas
• [Patent(Industrial Property Rights)] ユビキチンに対する人工抗体 (2022)
  • Inventor(s): 佐伯泰、遠藤彬則、河瀬泰子、林剛介、村上裕
  • Industrial Property Rights Holder: 公益財団法人東京都医学総合研究所
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2022-010877
  • Filing Date: 2022
• [Funded Workshop] Ubiquitin New Frontier "from Neo-Biology to Targeted Protein Degradation" (2022)
• [Funded Workshop] 第23回都医学研国際シンポジウム (2022)