| Budget Amount *help |
¥169,520,000 (Direct Cost: ¥130,400,000、Indirect Cost: ¥39,120,000)
Fiscal Year 2022: ¥30,680,000 (Direct Cost: ¥23,600,000、Indirect Cost: ¥7,080,000)
Fiscal Year 2021: ¥30,680,000 (Direct Cost: ¥23,600,000、Indirect Cost: ¥7,080,000)
Fiscal Year 2020: ¥30,680,000 (Direct Cost: ¥23,600,000、Indirect Cost: ¥7,080,000)
Fiscal Year 2019: ¥30,680,000 (Direct Cost: ¥23,600,000、Indirect Cost: ¥7,080,000)
Fiscal Year 2018: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
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| Outline of Final Research Achievements |
In this study, to understand the chromatin potential of the fertilized egg, quantitative and reconstructive analyses of nuclear, chromosomal, and chromatin dynamics after fertilization were performed using our own methodologies such as non-invasive live-cell imaging, artificial cell nucleus, and epigenome editing. We were able to construct artificial nuclei with nuclear transfer potential and heterochromatin structure in fertilized eggs by using long-stranded DNA as starting material. Epigenome editing revealed that DNA methylation in the centromere region is involved in fertilized egg-specific cell cycle regulation. We newly developed minimally invasive super-resolution imaging and succeeded in super-resolution observation of nuclear chromatin and chromosomes in living cells.
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