• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of repertoire dynamics of germ cell development

Planned Research

Project AreaEnsuring integrity in gametogenesis
Project/Area Number 18H05551
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute for Basic Biology

Principal Investigator

吉田 松生  基礎生物学研究所, 生殖細胞研究部門, 教授 (60294138)

Co-Investigator(Kenkyū-buntansha) 中村 隼明  広島大学, 統合生命科学研究科(生), 助教 (30613723)
Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Granted (Fiscal Year 2022)
Budget Amount *help
¥119,210,000 (Direct Cost: ¥91,700,000、Indirect Cost: ¥27,510,000)
Fiscal Year 2022: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2021: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2020: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000)
Fiscal Year 2019: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2018: ¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000)
Keywords生殖細胞 / レパートリー / 細胞系譜 / バーコーディング
Outline of Annual Research Achievements

2020年度は以下のような研究を遂行し、生殖細胞の発生様式の異なる哺乳類と鳥類における配偶子インテグリティ成立プロセスの解析を進めた。まず、2019年度までに確立した、マウス生殖細胞特異的に多数の異なる配列(バーコード)を導入する実験系を用いて、胎児生殖細胞にバーコードを導入し、発生の進んだ段階の生殖細胞および精子を介して次世代に伝わったバーコードのデータ取得を進めた。また、本解析に必要な情報である、胎児期の生殖細胞数を、3次元顕微鏡画像より計測するアルゴリズムを共同開発した。これらのデータを用いて、海外研究協力者とともに解析手法を開発しながら、クローンレパートリー数の動態解析を行った。さらに、2019年度までにマウス精巣を異なる温度で培養することで明らかにした、深部体温程度の温度上昇によって、精子形成、とくに減数分裂の進行が障害を受ける分子メカニズムの解明を進め、DNA二重鎖切断の修復および相同染色体の対合が障害されること、障害を受けた細胞は減数分裂チェックポイントによって除去されることを明らかにした(以上は、研究代表者吉田が行った)。鳥類においては、ニワトリ生殖細胞の培養条件を改善し、遺伝子導入の条件の検討を進めた (以上は、研究分担者中村が行った)。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

上記のように本研究は進行している。2019年度にコロナウイルス感染症の影響などで、計画以上に時間を要したバーコード導入や培養条件の最適化については、最適化した実験条件を用いて信頼できるデータを網羅的に取得することができた。以上から、概ね順調に進展していると判断する。

Strategy for Future Research Activity

基本的には、現在進行している研究を推進することで、本研究の目的の達成を目指す。それを通して、本研究領域の研究の進展に貢献する。マウス生殖細胞のレパートリー動態については、本年度までに取得したバーコード頻度についてのデータの取得を完了する。海外研究協力者とともに数理統計解析を行い、個体に含まれる生殖細胞レパートリーの変遷を高い精度で推定することを実現する。レパートリーが減少する段階が同定されれば、その時期の生殖細胞の不均一性を検討する。さらに、細胞系譜と遺伝子発現の情報を紐づけて解析する実験系を導入する。マウス精子形成の高温感受性については、高温で障害を受ける減数分裂のステップと分子マシナリーを絞り込む。深部体温程度の温度上昇に感応するメカニズムの解明を目指して、候補分子の各種変異体の解析を行う。鳥類では、ニワトリ生殖細胞への遺伝子導入効率とその培養効率を改善することで、遺伝子改変した安定細胞株樹立の効率化を図る。また、Brainbow 2.1ならびにdead end MCMが導入された遺伝子改変ニワトリの作出を進め、生殖細胞を複数の蛍光タンパク質でランダムにパルス標識することで、生殖細胞系譜の運命を追跡する。

Report

(3 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report

Research Products

(80 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (10 results) Journal Article (24 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 19 results,  Open Access: 21 results) Presentation (42 results) (of which Int'l Joint Research: 21 results,  Invited: 31 results) Book (3 results) Remarks (1 results)

  • [Int'l Joint Research] ドイツがん研究センター(DKFZ)/ハイデルベルク大学(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ロスリン研究所/ケンブリッジ大学(英国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] シンシナティ大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ミナス・ジェライス連邦大学(ブラジル)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ドイツがん研究センター(DKFZ)/ハイデルベルク大学(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ロスリン研究所/ケンブリッジ大学(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] シンシナティ大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ミナス・ジェライス連邦大学(ブラジル)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ドイツがん研究センター(DKFZ)/ハイデルベルク大学(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ロスリン研究所(英国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Regulation of spermatogenic stem cell homeostasis by mitogen competition in an open niche microenvironment2022

    • Author(s)
      Kitadate Yu、Yoshida Shosei
    • Journal Title

      Genes & Genetic Systems

      Volume: 97 Issue: 1 Pages: 15-25

    • DOI

      10.1266/ggs.21-00062

    • NAID

      130008158203

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2022-02-01
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 特集 組織幹細胞の共通性と特殊性 Ⅰ.幹細胞の自律性とニッチ依存性:Q.幹細胞の自己複製能とは何か? 精子幹細胞のランダムな挙動からニッチの役割を考える2021

    • Author(s)
      吉田 松生
    • Journal Title

      生体の科学

      Volume: 72 Issue: 2 Pages: 100-104

    • DOI

      10.11477/mf.2425201322

    • ISSN
      0370-9531, 1883-5503
    • Year and Date
      2021-04-15
    • Related Report
      2020 Annual Research Report
  • [Journal Article] A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis2021

    • Author(s)
      Nakagawa Toshinori、Jorg David J.、Watanabe Hitomi、Mizuno Seiya、Han Seungmin、Ikeda Tatsuro、Omatsu Yoshiki、Nishimura Keiko、Fujita Miyako、Takahashi Satoru、Kondoh Gen、Simons Benjamin D.、Yoshida Shosei、Nagasawa Takashi
    • Journal Title

      Cell Reports

      Volume: 37 Issue: 3 Pages: 109875-109875

    • DOI

      10.1016/j.celrep.2021.109875

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice2021

    • Author(s)
      Osawa Yuki, Murata Kazuya, Usui Miho, Kuba Yumeno, Le H Thu, Mikami Natsuki, Nakagawa Toshinori, Daitoku Yoko, Kato Kanako, Shawki H Hassan, Ikeda Yoshihisa, Kuno Akihiro, Morimoto Kento, Tanimoto Yoko, Dinh T T Huong, Yagami Ken-ichi, Ema Masatsugu, Yoshida Shosei, Takahashi Satoru, Mizuno Seiya, Sugiyama Fumihiro
    • Journal Title

      eLife

      Volume: 10

    • DOI

      10.7554/elife.59759

    • NAID

      120007190954

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice2021

    • Author(s)
      Nakamura Y, Jorg DJ, Kon Y, Simons BD, Yoshida S
    • Journal Title

      Cell Stem Cell

      Volume: 28 Issue: 8 Pages: 1-14

    • DOI

      10.1016/j.stem.2021.03.016

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Stem Cell Populations as Self-Renewing Many-Particle Systems2021

    • Author(s)
      David J. Jorg, Yu Kitadate, Shosei Yoshida, and Benjamin D. Simons
    • Journal Title

      Annual Review of Condensed Matter Physics

      Volume: 12 Issue: 1 Pages: 135-153

    • DOI

      10.1146/annurev-conmatphys-041720-125707

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye2021

    • Author(s)
      Y.H. Yeh, M. Hu, T. Nakagawa, A. Sakashita, S. Yoshida, S. Maezawa and S.H. Namekaw
    • Journal Title

      J Vis Exp

      Volume: - Issue: 167

    • DOI

      10.3791/61666

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 精子幹細胞研究の展開:見えてきた個のランダム性と集団の安定性2021

    • Author(s)
      吉田松生
    • Journal Title

      生化学

      Volume: -

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Journal Article] A Growth Performance and Nonlinear Growth Curve Functions of Large- and Normal-Sized Japanese Quail (<i>Coturnix japonica</i>)2021

    • Author(s)
      Haqani Mohammad I.、Kawamura Kensuke、Takenouchi Atsushi、Kabir Mohammad H.、Nakamura Yoshiaki、Ishikawa Akira、Tsudzuki Masaoki
    • Journal Title

      The Journal of Poultry Science

      Volume: 58 Issue: 2 Pages: 88-96

    • DOI

      10.2141/jpsa.0200020

    • NAID

      130008029804

    • ISSN
      1346-7395, 1349-0486
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mapping of Quantitative Trait Loci Controlling Egg-Quality and -Production Traits in Japanese Quail (Coturnix japonica) Using Restriction-Site Associated DNA Sequencing2021

    • Author(s)
      Haqani Mohammad Ibrahim、Nomura Shigeru、Nakano Michiharu、Goto Tatsuhiko、Nagano Atsushi J.、Takenouchi Atsushi、Nakamura Yoshiaki、Ishikawa Akira、Tsudzuki Masaoki
    • Journal Title

      Genes

      Volume: 12 Issue: 5 Pages: 735-735

    • DOI

      10.3390/genes12050735

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quantitative trait loci for growth-related traits in Japanese quail (Coturnix japonica) using restriction-site associated DNA sequencing2021

    • Author(s)
      Haqani Mohammad Ibrahim、Nomura Shigeru、Nakano Michiharu、Goto Tatsuhiko、Nagano Atsushi J.、Takenouchi Atsushi、Nakamura Yoshiaki、Ishikawa Akira、Tsudzuki Masaoki
    • Journal Title

      Molecular Genetics and Genomics

      Volume: 296 Issue: 5 Pages: 1147-1159

    • DOI

      10.1007/s00438-021-01806-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] EXOC1 regulates cell morphology of spermatogonia and spermatocytes in mice2021

    • Author(s)
      Osawa Y、Murata K、Usui M、Kuba Y、Le Hoai T、Mikami N、Nakagawa T、Daitoku Y、Kato K、Shawki Hossam H、Ikeda Y、Kuno A、Morimoto K、Tanimoto Y、Huong Dinh Tra T、Yagami K、Ema M、Yoshida S、Takahashi S、Mizuno S、Sugiyama F
    • Journal Title

      eLife

      Volume: -

    • DOI

      10.1101/2020.06.07.139030

    • Related Report
      2019 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] 精子幹細胞のランダムな挙動からニッチの役割を考える2021

    • Author(s)
      吉田松生
    • Journal Title

      生体の科学

      Volume: 72 Pages: 1-5

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Spermatogonial asynchrony in Tex14 mutant mice lacking intercellular bridges.2020

    • Author(s)
      Rezende-Melo, C.A., Caldeira-Brant, A.L. Drumond-Bock, , A.L. Buchold, , G.M., Shetty, G., Almeida, F.R.C.L. Matzuk, M.M., Hara, K.. Yoshida, S.. Meistrich, M.L. Chiarini-Garcia, H.:
    • Journal Title

      Reproduction

      Volume: 160 Issue: 2 Pages: 205-215

    • DOI

      10.1530/rep-20-0118

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tracing the cellular basis of islet specification in mouse pancreas2020

    • Author(s)
      Sznurkowska Magdalena K.、Hannezo Edouard、Azzarelli Roberta、Chatzeli Lemonia、Ikeda Tatsuro、Yoshida Shosei、Philpott Anna、Simons Benjamin D.
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 5037-5037

    • DOI

      10.1038/s41467-020-18837-3

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mouse spermatogenesis reflects the unity and diversity of tissue stem cell-niche systems.2020

    • Author(s)
      Yoshida, S.
    • Journal Title

      Cold Spring Harbor Perspectives in Biology

      Volume: - Issue: 12 Pages: a036186-a036186

    • DOI

      10.1101/cshperspect.a036186

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heterogeneous, dynamic, and stochastic nature of mammalian spermatogenic stem cells.2019

    • Author(s)
      Yoshida, S.
    • Journal Title

      Curr Top Dev Biol

      Volume: 135 Pages: 245-285

    • DOI

      10.1016/bs.ctdb.2019.04.008

    • ISBN
      9780128097526
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Wnt produced by stretched roof-plate cells is required for the promotion of cell proliferation around the central canal of the spinal cord2019

    • Author(s)
      Shinozuka Takuma、Takada Ritsuko、Yoshida Shosei、Yonemura Shigenobu、Takada Shinji
    • Journal Title

      Development

      Volume: 146 Issue: 2 Pages: 159343-159343

    • DOI

      10.1242/dev.159343

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche2019

    • Author(s)
      Kitadate Y., Jorg DJ., Tokue M,. Maruyama A., Ishikawa R., Tsuchiya S., Segi-Nishida E., Nakagawa T., Uchida A., Kimura-Yoshida C., Mizuno S., Sugiyama F., Azami T., Ema M., Noda C., Kobayashi S., Matsuo I., Kanai Y., Nagasawa T., Sugimoto Y., Takahashi S., Simons BD., Yoshida S.
    • Journal Title

      Cell Stem Cell

      Volume: 24 Issue: 1 Pages: 79-92

    • DOI

      10.1016/j.stem.2018.11.013

    • NAID

      120007128265

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Developmental stages of the blue-breasted quail (Coturnix chinensis).2019

    • Author(s)
      Y. Nakamura, Y. Nakane and M. Tsudzuki
    • Journal Title

      Anim Sci J

      Volume: 90 Issue: 1 Pages: 35-48

    • DOI

      10.1111/asj.13119

    • NAID

      120006554901

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Skeletal development in blue-breasted quail embryos.2019

    • Author(s)
      Y. Nakamura, Y. Nakane and M. Tsudzuki
    • Journal Title

      Anim Sci J

      Volume: 90 Issue: 3 Pages: 353-365

    • DOI

      10.1111/asj.13159

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility.2018

    • Author(s)
      S. Sakamoto, D. Thumkeo, H. Ohta, Z. Zhang, S.R. Huang, P. Kanchenawong, T. Fuu, S. Watanabe, K. Shimada, Y. Fujihara, S. Yoshida, M. Ikawa, N. Watanabe, M. Saitou and S. Narumiya
    • Journal Title

      Plos Biology

      Volume: 16 Issue: 9 Pages: e2004874-e2004874

    • DOI

      10.1371/journal.pbio.2004874

    • NAID

      120006531249

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Open niche regulation of mouse spermatogenic stem cells2018

    • Author(s)
      S. Yoshida
    • Journal Title

      Dev Growth Differ

      Volume: 60 Issue: 9 Pages: 542-552

    • DOI

      10.1111/dgd.12574

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 精子幹細胞と精巣の開放型ニッチ 特集「幹細胞の恒常性維持とその破綻」2018

    • Author(s)
      吉田松生
    • Journal Title

      月刊細胞

      Volume: 50 Pages: 15-18

    • NAID

      40021598195

    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Presentation] Live-imaging the stem cells and spatiotemporal pattern of spermatogenesis in the mouse testis2022

    • Author(s)
      Shosei Yoshida
    • Organizer
      ISSCR webinar series, Understanding Stem Cells & Cellular Processes Through Imaging
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マウス精子形成における減数分裂の温度感受性2022

    • Author(s)
      吉田松生
    • Organizer
      大阪大学蛋白質研究所セミナー 生殖細胞・減数分裂研究の最前線
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 精子形成の周期と波の可視化2022

    • Author(s)
      吉田松生
    • Organizer
      認識と形成2022 冬の討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ジメチルスルホキシドを用いたニワトリ始原生殖細胞の凍結保存液の開発2022

    • Author(s)
      濵井奈津子,丹所祐貴,大岡雪乃,平野茉葉,都築政起,中村隼明
    • Organizer
      日本家禽学会2022年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 精子幹細胞: そのランダムな挙動と移植による妊孕性の回復2021

    • Author(s)
      吉田松生
    • Organizer
      第109回日本泌尿器科学会総会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 機能的にヘテロジーニアスなマウス精子幹細胞集団を維持する、確率的な状態の転移2021

    • Author(s)
      中川俊徳、吉田松生
    • Organizer
      第114回日本繁殖生物学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Clonal dynamics of developing germ cells in murine testes2021

    • Author(s)
      Tatsuro Ikeda, Langhinrichs Maurice, Nizharadze Tamar, Hofer Thomas, Rodewald Hans-Reimer, Shosei Yoshida
    • Organizer
      92nd Annual Meeting of the ZSJ
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of Spermatogonial Stem Cell Dynamics in the Testis2021

    • Author(s)
      Shosei Yoshida
    • Organizer
      CRH Reproduction Seminar, virtual seminar series on Germline Development and Function
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamics and regulation of sperm stem cells in the mouse testis2021

    • Author(s)
      Shosei Yoshida
    • Organizer
      RIMS Workshop "Biofluid Mechanics of Reproduction"
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fertility restoration by tuning the stochastic fate of transplanted spermatogonial stem cells in mouse2021

    • Author(s)
      Yoshiaki Nakamura, David Jorg, Yayoi Kon, Benjamin Simons, and Shosei Yoshida
    • Organizer
      21st European Testis Workshop
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multistate dynamics of stem cells in steady-state mouse spermatogenesis2021

    • Author(s)
      Toshinori Nakagawa, David Jorg, Benjamin Simons, and Shosei Yoshida
    • Organizer
      第18回幹細胞シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] Restoration of fertility by tuning the post-transplantation fate of mouse spermatogonial stem cells2021

    • Author(s)
      Yoshiaki Nakamura
    • Organizer
      第18回幹細胞シンポジウム
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ニワトリ始原生殖細胞の培養と凍結保存2021

    • Author(s)
      中村隼明
    • Organizer
      第5回HiHA Young Researchers Workshop (広島大学健康長寿拠点) NBRPオンラインワークショップ バイオリソースの先端技術まるわかり
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 精子幹細胞移植の進展と畜産への応用2021

    • Author(s)
      中村隼明
    • Organizer
      第130回日本畜産学会大会 パラレルシンポジウムII
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Cryobanking of chicken through manipulation of primordial gem cells2021

    • Author(s)
      中村隼明
    • Organizer
      Workshop on Avian Biodiversity (The East Asia Science and Innovation Area Joint Research Program)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Manipulation of primordial germ cells for conservation and breeding of endangered birds2021

    • Author(s)
      Yoshiaki Nakamura
    • Organizer
      Avian Genetic Rescue Meeting (Revive & Restore)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 精子幹細胞研究の展開:見えてきた個のランダム性と集団の安定性2021

    • Author(s)
      吉田松生
    • Organizer
      第3回せとうちART研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 龍神地鶏初期発生における始原生殖細胞数の調査2021

    • Author(s)
      小出茅洋, 濵井奈津子, 橋本典和, 都築政起, 中村隼明
    • Organizer
      2021年度日本家禽学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ニワトリ始原生殖細胞に適した凍結保存液の開発2021

    • Author(s)
      濵井奈津子, 小出茅洋, 都築政起, 中村 隼明
    • Organizer
      日本畜産学会第128回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Maintenace of homeostasis and highjack by non-neutral stem cell clones in mouse spermatogenesis2020

    • Author(s)
      Shosei Yoshida
    • Organizer
      Cold Spring Harbor Laboratory Meeting on Germ Cells
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sperm production with minimal mutations. S4: Developmental mechanisms underlying sexual reproduction2020

    • Author(s)
      Shosei Yoshida
    • Organizer
      JSDB Online Trial Meeting 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Maintenace of homeostasis and highjack by non-neutral stem cell clones in mouse spermatogenesis.2020

    • Author(s)
      吉田松生
    • Organizer
      Cold Spring Harbor Laboratory Meeting on Germ Cells
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sperm production with minimal mutations. S4: Developmental mechanisms underlying sexual reproduction2020

    • Author(s)
      吉田松生
    • Organizer
      JSDB Online Trial Meeting 2020
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 宿主精巣に移植した精子幹細胞はストカスティックな運命を辿る2020

    • Author(s)
      中村隼明,David J Jorg,Benjamin D Simons,吉田松生
    • Organizer
      第113回日本繁殖生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マウス精子形成の温度チェックポイントのex vivo 培養による解明2019

    • Author(s)
      吉田松生
    • Organizer
      Biothermology Workshop 2019’ Annual Workshop
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Robust spermatogenesis is supported by random behavior and simple regulation of stem cells2019

    • Author(s)
      吉田松生
    • Organizer
      第42回日本分子生物学会シンポジウム 4S-11 “Robustness of germline lineage”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Temperature sensitivity of mouse spermatogenesis.2019

    • Author(s)
      吉田松生
    • Organizer
      Memorial Conference for Dr. Goro Eguchi in Kumamoto
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 精子幹細胞の集団動態と次世代へ伝わる変異へのインパクト2019

    • Author(s)
      吉田松生
    • Organizer
      日本遺伝学会第91回大会シンポジウム“遺伝学会・新学術「配偶子インテグリティ」共催シンポ:「個体を潜り抜けるための遺伝戦略」”
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] マウス精子形成幹細胞の気ままな振る舞いとその制御2019

    • Author(s)
      吉田松生
    • Organizer
      2019遺伝研研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ひとつひとつの幹細胞のランダムなふるまいが安定した精子形成を支える2019

    • Author(s)
      吉田松生
    • Organizer
      第17回幹細胞シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Unique differentiation processes support the sharp seasonal response of Japanese quail spermatogenesis.2019

    • Author(s)
      吉田松生
    • Organizer
      The annual meeting of the Japanese Society of Developmental Biologists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Open niche regulation of spermatogenic stem cells in the mouse testis.2018

    • Author(s)
      S. Yoshida
    • Organizer
      The 5th Conference of Frontiers in Reproductive Biology,
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Rapid and robust seasonal breeding in Japanese quail supported by unique process of spermatogenesis.2018

    • Author(s)
      S. Yoshida
    • Organizer
      Cold Spring Harbor Laboratory Meeting on Germ Cells
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Towards a Better Understanding of Spermatogenic Stem Cells.2018

    • Author(s)
      S. Yoshida
    • Organizer
      The Society for the Study of Reproduction 51st Annual Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sperm stem cells: their context-dependent behavior.2018

    • Author(s)
      S. Yoshida
    • Organizer
      ISSCR 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 精子形成幹細胞のホメオスタシス維持システムとその経時変化2018

    • Author(s)
      吉田松生
    • Organizer
      千里ライフサイエンスセミナーM1<老化メカニズムと疾患制御>
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Flexible and stochastic behavior of spermatogenic stem cells.2018

    • Author(s)
      S. Yoshida
    • Organizer
      The 20th European Testis Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] For a Better Understanding of Spermatogenic Stem Cell Dynamics Over Time.2018

    • Author(s)
      S. Yoshida
    • Organizer
      11th NYRA Meeting, preceding the 20th European Testis Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 精子を作る細胞たち:個のランダム性が集団を支える2018

    • Author(s)
      吉田松生
    • Organizer
      大隅基礎科学創成財団「第二回創発セミナー」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 生殖細胞の凍結・移植による家禽遺伝資源の長期保存2018

    • Author(s)
      中村隼明
    • Organizer
      日本農芸化学会2018年度大会 シンポジウム「鳥類の農芸化学」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Embryonic Development of the Blue-Breasted Quail (Coturnix chinensis).2018

    • Author(s)
      Nakamura Y, Nakane Y, Tsudzuki M
    • Organizer
      Asian Australasian Animal Production Congress 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 「家禽類および齧歯類における生殖細胞の移植を用いたキメラ作製技法」2018

    • Author(s)
      中村隼明
    • Organizer
      第111回日本繁殖生物学会大会 大会企画シンポジウム1「キメラ動物作製技術の応用」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Book] 遺伝学の百科事典2022

    • Author(s)
      日本遺伝学会
    • Total Pages
      690
    • Publisher
      丸善出版
    • ISBN
      9784621306604
    • Related Report
      2020 Annual Research Report
  • [Book] SDGsに向けた生物生産学入門2021

    • Author(s)
      三本木 至宏、上田 晃弘、杉野 利久、鈴木 卓弥、冨山 毅、船戸 耕一
    • Total Pages
      256
    • Publisher
      共立出版
    • ISBN
      9784320058316
    • Related Report
      2020 Annual Research Report
  • [Book] Regulatory mechanism of spermatogenic stem cells in mice: their dynamic and context-dependent behavior: PartⅠDivesity in Reproductive Stragegies and Germ Cell Differentiation Mechanisms, Animal Diverdity and Gnerality, “Reproductive and Developmental Strategies”2018

    • Author(s)
      S. Yoshida
    • Total Pages
      789
    • Publisher
      Springer Japan KK
    • Related Report
      2018 Annual Research Report
  • [Remarks] 基礎生物学研究所 吉田研究室

    • URL

      http://www.nibb.ac.jp/germcell/

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report 2018 Annual Research Report

URL: 

Published: 2018-07-20   Modified: 2022-12-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi