Analysis of repertoire dynamics of germ cell development

• Project Area: Ensuring integrity in gametogenesis
• Project/Area Number: 18H05551
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology
• Principal Investigator: 吉田 松生 基礎生物学研究所, 生殖細胞研究部門, 教授 (60294138)
• Co-Investigator(Kenkyū-buntansha): 中村 隼明 広島大学, 統合生命科学研究科(生), 助教 (30613723)
• Project Period (FY): 2018-06-29 – 2023-03-31
• Project Status: Granted (Fiscal Year 2022)
• Budget Amount: ¥119,210,000 (Direct Cost: ¥91,700,000、Indirect Cost: ¥27,510,000); Fiscal Year 2022: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000); Fiscal Year 2021: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000); Fiscal Year 2020: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000); Fiscal Year 2019: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000); Fiscal Year 2018: ¥30,030,000 (Direct Cost: ¥23,100,000、Indirect Cost: ¥6,930,000)
• Keywords: 生殖細胞 / レパートリー / 細胞系譜 / バーコーディング

Outline of Annual Research Achievements

2020年度は以下のような研究を遂行し、生殖細胞の発生様式の異なる哺乳類と鳥類における配偶子インテグリティ成立プロセスの解析を進めた。まず、2019年度までに確立した、マウス生殖細胞特異的に多数の異なる配列（バーコード）を導入する実験系を用いて、胎児生殖細胞にバーコードを導入し、発生の進んだ段階の生殖細胞および精子を介して次世代に伝わったバーコードのデータ取得を進めた。また、本解析に必要な情報である、胎児期の生殖細胞数を、３次元顕微鏡画像より計測するアルゴリズムを共同開発した。これらのデータを用いて、海外研究協力者とともに解析手法を開発しながら、クローンレパートリー数の動態解析を行った。さらに、2019年度までにマウス精巣を異なる温度で培養することで明らかにした、深部体温程度の温度上昇によって、精子形成、とくに減数分裂の進行が障害を受ける分子メカニズムの解明を進め、DNA二重鎖切断の修復および相同染色体の対合が障害されること、障害を受けた細胞は減数分裂チェックポイントによって除去されることを明らかにした（以上は、研究代表者吉田が行った）。鳥類においては、ニワトリ生殖細胞の培養条件を改善し、遺伝子導入の条件の検討を進めた (以上は、研究分担者中村が行った)。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

上記のように本研究は進行している。2019年度にコロナウイルス感染症の影響などで、計画以上に時間を要したバーコード導入や培養条件の最適化については、最適化した実験条件を用いて信頼できるデータを網羅的に取得することができた。以上から、概ね順調に進展していると判断する。

Strategy for Future Research Activity

基本的には、現在進行している研究を推進することで、本研究の目的の達成を目指す。それを通して、本研究領域の研究の進展に貢献する。マウス生殖細胞のレパートリー動態については、本年度までに取得したバーコード頻度についてのデータの取得を完了する。海外研究協力者とともに数理統計解析を行い、個体に含まれる生殖細胞レパートリーの変遷を高い精度で推定することを実現する。レパートリーが減少する段階が同定されれば、その時期の生殖細胞の不均一性を検討する。さらに、細胞系譜と遺伝子発現の情報を紐づけて解析する実験系を導入する。マウス精子形成の高温感受性については、高温で障害を受ける減数分裂のステップと分子マシナリーを絞り込む。深部体温程度の温度上昇に感応するメカニズムの解明を目指して、候補分子の各種変異体の解析を行う。鳥類では、ニワトリ生殖細胞への遺伝子導入効率とその培養効率を改善することで、遺伝子改変した安定細胞株樹立の効率化を図る。また、Brainbow 2.1ならびにdead end MCMが導入された遺伝子改変ニワトリの作出を進め、生殖細胞を複数の蛍光タンパク質でランダムにパルス標識することで、生殖細胞系譜の運命を追跡する。

Research Products

• [Int'l Joint Research] ドイツがん研究センター（DKFZ）/ハイデルベルク大学(ドイツ)
• [Int'l Joint Research] ロスリン研究所/ケンブリッジ大学(英国)
• [Int'l Joint Research] シンシナティ大学(米国)
• [Int'l Joint Research] ミナス・ジェライス連邦大学(ブラジル)
• [Int'l Joint Research] ドイツがん研究センター（DKFZ）/ハイデルベルク大学(ドイツ)
• [Int'l Joint Research] ロスリン研究所/ケンブリッジ大学(英国)
• [Int'l Joint Research] シンシナティ大学(米国)
• [Int'l Joint Research] ミナス・ジェライス連邦大学(ブラジル)
• [Int'l Joint Research] ドイツがん研究センター（DKFZ）/ハイデルベルク大学(ドイツ)
• [Int'l Joint Research] ロスリン研究所(英国)
• [Journal Article] Regulation of spermatogenic stem cell homeostasis by mitogen competition in an open niche microenvironment (2022)
  • Author(s): Kitadate Yu、Yoshida Shosei
  • Journal Title: Genes & Genetic Systems Volume: 97 Issue: 1 Pages: 15-25
  • DOI: 10.1266/ggs.21-00062
  • NAID: 130008158203
  • ISSN: 1341-7568, 1880-5779
  • Year and Date: 2022-02-01
  • Peer Reviewed / Open Access
• [Journal Article] 特集 組織幹細胞の共通性と特殊性 Ⅰ.幹細胞の自律性とニッチ依存性:Q.幹細胞の自己複製能とは何か? 精子幹細胞のランダムな挙動からニッチの役割を考える (2021)
  • Author(s): 吉田 松生
  • Journal Title: 生体の科学 Volume: 72 Issue: 2 Pages: 100-104
  • DOI: 10.11477/mf.2425201322
  • ISSN: 0370-9531, 1883-5503
  • Year and Date: 2021-04-15
• [Journal Article] A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis (2021)
  • Author(s): Nakagawa Toshinori、Jorg David J.、Watanabe Hitomi、Mizuno Seiya、Han Seungmin、Ikeda Tatsuro、Omatsu Yoshiki、Nishimura Keiko、Fujita Miyako、Takahashi Satoru、Kondoh Gen、Simons Benjamin D.、Yoshida Shosei、Nagasawa Takashi
  • Journal Title: Cell Reports Volume: 37 Issue: 3 Pages: 109875-109875
  • DOI: 10.1016/j.celrep.2021.109875
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice (2021)
  • Author(s): Osawa Yuki, Murata Kazuya, Usui Miho, Kuba Yumeno, Le H Thu, Mikami Natsuki, Nakagawa Toshinori, Daitoku Yoko, Kato Kanako, Shawki H Hassan, Ikeda Yoshihisa, Kuno Akihiro, Morimoto Kento, Tanimoto Yoko, Dinh T T Huong, Yagami Ken-ichi, Ema Masatsugu, Yoshida Shosei, Takahashi Satoru, Mizuno Seiya, Sugiyama Fumihiro
  • Journal Title: eLife Volume: 10
  • DOI: 10.7554/elife.59759
  • NAID: 120007190954
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice (2021)
  • Author(s): Nakamura Y, Jorg DJ, Kon Y, Simons BD, Yoshida S
  • Journal Title: Cell Stem Cell Volume: 28 Issue: 8 Pages: 1-14
  • DOI: 10.1016/j.stem.2021.03.016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Stem Cell Populations as Self-Renewing Many-Particle Systems (2021)
  • Author(s): David J. Jorg, Yu Kitadate, Shosei Yoshida, and Benjamin D. Simons
  • Journal Title: Annual Review of Condensed Matter Physics Volume: 12 Issue: 1 Pages: 135-153
  • DOI: 10.1146/annurev-conmatphys-041720-125707
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye (2021)
  • Author(s): Y.H. Yeh, M. Hu, T. Nakagawa, A. Sakashita, S. Yoshida, S. Maezawa and S.H. Namekaw
  • Journal Title: J Vis Exp Volume: - Issue: 167
  • DOI: 10.3791/61666
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 精子幹細胞研究の展開：見えてきた個のランダム性と集団の安定性 (2021)
  • Author(s): 吉田松生
  • Journal Title: 生化学 Volume: -
• [Journal Article] A Growth Performance and Nonlinear Growth Curve Functions of Large- and Normal-Sized Japanese Quail (<i>Coturnix japonica</i>) (2021)
  • Author(s): Haqani Mohammad I.、Kawamura Kensuke、Takenouchi Atsushi、Kabir Mohammad H.、Nakamura Yoshiaki、Ishikawa Akira、Tsudzuki Masaoki
  • Journal Title: The Journal of Poultry Science Volume: 58 Issue: 2 Pages: 88-96
  • DOI: 10.2141/jpsa.0200020
  • NAID: 130008029804
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mapping of Quantitative Trait Loci Controlling Egg-Quality and -Production Traits in Japanese Quail (Coturnix japonica) Using Restriction-Site Associated DNA Sequencing (2021)
  • Author(s): Haqani Mohammad Ibrahim、Nomura Shigeru、Nakano Michiharu、Goto Tatsuhiko、Nagano Atsushi J.、Takenouchi Atsushi、Nakamura Yoshiaki、Ishikawa Akira、Tsudzuki Masaoki
  • Journal Title: Genes Volume: 12 Issue: 5 Pages: 735-735
  • DOI: 10.3390/genes12050735
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantitative trait loci for growth-related traits in Japanese quail (Coturnix japonica) using restriction-site associated DNA sequencing (2021)
  • Author(s): Haqani Mohammad Ibrahim、Nomura Shigeru、Nakano Michiharu、Goto Tatsuhiko、Nagano Atsushi J.、Takenouchi Atsushi、Nakamura Yoshiaki、Ishikawa Akira、Tsudzuki Masaoki
  • Journal Title: Molecular Genetics and Genomics Volume: 296 Issue: 5 Pages: 1147-1159
  • DOI: 10.1007/s00438-021-01806-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] EXOC1 regulates cell morphology of spermatogonia and spermatocytes in mice (2021)
  • Author(s): Osawa Y、Murata K、Usui M、Kuba Y、Le Hoai T、Mikami N、Nakagawa T、Daitoku Y、Kato K、Shawki Hossam H、Ikeda Y、Kuno A、Morimoto K、Tanimoto Y、Huong Dinh Tra T、Yagami K、Ema M、Yoshida S、Takahashi S、Mizuno S、Sugiyama F
  • Journal Title: eLife Volume: -
  • DOI: 10.1101/2020.06.07.139030
  • Open Access / Int'l Joint Research
• [Journal Article] 精子幹細胞のランダムな挙動からニッチの役割を考える (2021)
  • Author(s): 吉田松生
  • Journal Title: 生体の科学 Volume: 72 Pages: 1-5
• [Journal Article] Spermatogonial asynchrony in Tex14 mutant mice lacking intercellular bridges. (2020)
  • Author(s): Rezende-Melo, C.A., Caldeira-Brant, A.L. Drumond-Bock, , A.L. Buchold, , G.M., Shetty, G., Almeida, F.R.C.L. Matzuk, M.M., Hara, K.. Yoshida, S.. Meistrich, M.L. Chiarini-Garcia, H.:
  • Journal Title: Reproduction Volume: 160 Issue: 2 Pages: 205-215
  • DOI: 10.1530/rep-20-0118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tracing the cellular basis of islet specification in mouse pancreas (2020)
  • Author(s): Sznurkowska Magdalena K.、Hannezo Edouard、Azzarelli Roberta、Chatzeli Lemonia、Ikeda Tatsuro、Yoshida Shosei、Philpott Anna、Simons Benjamin D.
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 5037-5037
  • DOI: 10.1038/s41467-020-18837-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mouse spermatogenesis reflects the unity and diversity of tissue stem cell-niche systems. (2020)
  • Author(s): Yoshida, S.
  • Journal Title: Cold Spring Harbor Perspectives in Biology Volume: - Issue: 12 Pages: a036186-a036186
  • DOI: 10.1101/cshperspect.a036186
  • Peer Reviewed / Open Access
• [Journal Article] Heterogeneous, dynamic, and stochastic nature of mammalian spermatogenic stem cells. (2019)
  • Author(s): Yoshida, S.
  • Journal Title: Curr Top Dev Biol Volume: 135 Pages: 245-285
  • DOI: 10.1016/bs.ctdb.2019.04.008
  • ISBN: 9780128097526
  • Peer Reviewed / Open Access
• [Journal Article] Wnt produced by stretched roof-plate cells is required for the promotion of cell proliferation around the central canal of the spinal cord (2019)
  • Author(s): Shinozuka Takuma、Takada Ritsuko、Yoshida Shosei、Yonemura Shigenobu、Takada Shinji
  • Journal Title: Development Volume: 146 Issue: 2 Pages: 159343-159343
  • DOI: 10.1242/dev.159343
  • Peer Reviewed / Open Access
• [Journal Article] Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche (2019)
  • Author(s): Kitadate Y., Jorg DJ., Tokue M,. Maruyama A., Ishikawa R., Tsuchiya S., Segi-Nishida E., Nakagawa T., Uchida A., Kimura-Yoshida C., Mizuno S., Sugiyama F., Azami T., Ema M., Noda C., Kobayashi S., Matsuo I., Kanai Y., Nagasawa T., Sugimoto Y., Takahashi S., Simons BD., Yoshida S.
  • Journal Title: Cell Stem Cell Volume: 24 Issue: 1 Pages: 79-92
  • DOI: 10.1016/j.stem.2018.11.013
  • NAID: 120007128265
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Developmental stages of the blue-breasted quail (Coturnix chinensis). (2019)
  • Author(s): Y. Nakamura, Y. Nakane and M. Tsudzuki
  • Journal Title: Anim Sci J Volume: 90 Issue: 1 Pages: 35-48
  • DOI: 10.1111/asj.13119
  • NAID: 120006554901
  • Peer Reviewed / Open Access
• [Journal Article] Skeletal development in blue-breasted quail embryos. (2019)
  • Author(s): Y. Nakamura, Y. Nakane and M. Tsudzuki
  • Journal Title: Anim Sci J Volume: 90 Issue: 3 Pages: 353-365
  • DOI: 10.1111/asj.13159
  • Peer Reviewed / Open Access
• [Journal Article] mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility. (2018)
  • Author(s): S. Sakamoto, D. Thumkeo, H. Ohta, Z. Zhang, S.R. Huang, P. Kanchenawong, T. Fuu, S. Watanabe, K. Shimada, Y. Fujihara, S. Yoshida, M. Ikawa, N. Watanabe, M. Saitou and S. Narumiya
  • Journal Title: Plos Biology Volume: 16 Issue: 9 Pages: e2004874-e2004874
  • DOI: 10.1371/journal.pbio.2004874
  • NAID: 120006531249
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Open niche regulation of mouse spermatogenic stem cells (2018)
  • Author(s): S. Yoshida
  • Journal Title: Dev Growth Differ Volume: 60 Issue: 9 Pages: 542-552
  • DOI: 10.1111/dgd.12574
  • Peer Reviewed / Open Access
• [Journal Article] 精子幹細胞と精巣の開放型ニッチ 特集「幹細胞の恒常性維持とその破綻」 (2018)
  • Author(s): 吉田松生
  • Journal Title: 月刊細胞 Volume: 50 Pages: 15-18
  • NAID: 40021598195
  • Open Access
• [Presentation] Live-imaging the stem cells and spatiotemporal pattern of spermatogenesis in the mouse testis (2022)
  • Author(s): Shosei Yoshida
  • Organizer: ISSCR webinar series, Understanding Stem Cells & Cellular Processes Through Imaging
  • Int'l Joint Research / Invited
• [Presentation] マウス精子形成における減数分裂の温度感受性 (2022)
  • Author(s): 吉田松生
  • Organizer: 大阪大学蛋白質研究所セミナー 生殖細胞・減数分裂研究の最前線
  • Invited
• [Presentation] 精子形成の周期と波の可視化 (2022)
  • Author(s): 吉田松生
  • Organizer: 認識と形成2022 冬の討論会
• [Presentation] ジメチルスルホキシドを用いたニワトリ始原生殖細胞の凍結保存液の開発 (2022)
  • Author(s): 濵井奈津子，丹所祐貴，大岡雪乃，平野茉葉，都築政起，中村隼明
  • Organizer: 日本家禽学会2022年度春季大会
• [Presentation] 精子幹細胞: そのランダムな挙動と移植による妊孕性の回復 (2021)
  • Author(s): 吉田松生
  • Organizer: 第109回日本泌尿器科学会総会
  • Invited
• [Presentation] 機能的にヘテロジーニアスなマウス精子幹細胞集団を維持する、確率的な状態の転移 (2021)
  • Author(s): 中川俊徳、吉田松生
  • Organizer: 第114回日本繁殖生物学会
• [Presentation] Clonal dynamics of developing germ cells in murine testes (2021)
  • Author(s): Tatsuro Ikeda, Langhinrichs Maurice, Nizharadze Tamar, Hofer Thomas, Rodewald Hans-Reimer, Shosei Yoshida
  • Organizer: 92nd Annual Meeting of the ZSJ
  • Int'l Joint Research
• [Presentation] Regulation of Spermatogonial Stem Cell Dynamics in the Testis (2021)
  • Author(s): Shosei Yoshida
  • Organizer: CRH Reproduction Seminar, virtual seminar series on Germline Development and Function
  • Int'l Joint Research / Invited
• [Presentation] Dynamics and regulation of sperm stem cells in the mouse testis (2021)
  • Author(s): Shosei Yoshida
  • Organizer: RIMS Workshop "Biofluid Mechanics of Reproduction"
  • Int'l Joint Research / Invited
• [Presentation] Fertility restoration by tuning the stochastic fate of transplanted spermatogonial stem cells in mouse (2021)
  • Author(s): Yoshiaki Nakamura, David Jorg, Yayoi Kon, Benjamin Simons, and Shosei Yoshida
  • Organizer: 21st European Testis Workshop
  • Int'l Joint Research / Invited
• [Presentation] Multistate dynamics of stem cells in steady-state mouse spermatogenesis (2021)
  • Author(s): Toshinori Nakagawa, David Jorg, Benjamin Simons, and Shosei Yoshida
  • Organizer: 第18回幹細胞シンポジウム
• [Presentation] Restoration of fertility by tuning the post-transplantation fate of mouse spermatogonial stem cells (2021)
  • Author(s): Yoshiaki Nakamura
  • Organizer: 第18回幹細胞シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] ニワトリ始原生殖細胞の培養と凍結保存 (2021)
  • Author(s): 中村隼明
  • Organizer: 第5回HiHA Young Researchers Workshop (広島大学健康長寿拠点) NBRPオンラインワークショップ バイオリソースの先端技術まるわかり
  • Invited
• [Presentation] 精子幹細胞移植の進展と畜産への応用 (2021)
  • Author(s): 中村隼明
  • Organizer: 第130回日本畜産学会大会 パラレルシンポジウムII
  • Invited
• [Presentation] Cryobanking of chicken through manipulation of primordial gem cells (2021)
  • Author(s): 中村隼明
  • Organizer: Workshop on Avian Biodiversity (The East Asia Science and Innovation Area Joint Research Program)
  • Int'l Joint Research / Invited
• [Presentation] Manipulation of primordial germ cells for conservation and breeding of endangered birds (2021)
  • Author(s): Yoshiaki Nakamura
  • Organizer: Avian Genetic Rescue Meeting (Revive & Restore)
  • Int'l Joint Research / Invited
• [Presentation] 精子幹細胞研究の展開：見えてきた個のランダム性と集団の安定性 (2021)
  • Author(s): 吉田松生
  • Organizer: 第3回せとうちART研究会
  • Invited
• [Presentation] 龍神地鶏初期発生における始原生殖細胞数の調査 (2021)
  • Author(s): 小出茅洋, 濵井奈津子, 橋本典和, 都築政起, 中村隼明
  • Organizer: 2021年度日本家禽学会春季大会
• [Presentation] ニワトリ始原生殖細胞に適した凍結保存液の開発 (2021)
  • Author(s): 濵井奈津子, 小出茅洋, 都築政起, 中村 隼明
  • Organizer: 日本畜産学会第128回大会
• [Presentation] Maintenace of homeostasis and highjack by non-neutral stem cell clones in mouse spermatogenesis (2020)
  • Author(s): Shosei Yoshida
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells
  • Int'l Joint Research / Invited
• [Presentation] Sperm production with minimal mutations. S4: Developmental mechanisms underlying sexual reproduction (2020)
  • Author(s): Shosei Yoshida
  • Organizer: JSDB Online Trial Meeting 2020
  • Int'l Joint Research / Invited
• [Presentation] Maintenace of homeostasis and highjack by non-neutral stem cell clones in mouse spermatogenesis. (2020)
  • Author(s): 吉田松生
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells
  • Int'l Joint Research / Invited
• [Presentation] Sperm production with minimal mutations. S4: Developmental mechanisms underlying sexual reproduction (2020)
  • Author(s): 吉田松生
  • Organizer: JSDB Online Trial Meeting 2020
  • Int'l Joint Research / Invited
• [Presentation] 宿主精巣に移植した精子幹細胞はストカスティックな運命を辿る (2020)
  • Author(s): 中村隼明，David J Jorg,Benjamin D Simons,吉田松生
  • Organizer: 第113回日本繁殖生物学会
• [Presentation] マウス精子形成の温度チェックポイントのex vivo 培養による解明 (2019)
  • Author(s): 吉田松生
  • Organizer: Biothermology Workshop 2019’ Annual Workshop
  • Invited
• [Presentation] Robust spermatogenesis is supported by random behavior and simple regulation of stem cells (2019)
  • Author(s): 吉田松生
  • Organizer: 第42回日本分子生物学会シンポジウム 4S-11 “Robustness of germline lineage”
  • Int'l Joint Research / Invited
• [Presentation] Temperature sensitivity of mouse spermatogenesis. (2019)
  • Author(s): 吉田松生
  • Organizer: Memorial Conference for Dr. Goro Eguchi in Kumamoto
  • Invited
• [Presentation] 精子幹細胞の集団動態と次世代へ伝わる変異へのインパクト (2019)
  • Author(s): 吉田松生
  • Organizer: 日本遺伝学会第91回大会シンポジウム“遺伝学会・新学術「配偶子インテグリティ」共催シンポ：「個体を潜り抜けるための遺伝戦略」”
  • Invited
• [Presentation] マウス精子形成幹細胞の気ままな振る舞いとその制御 (2019)
  • Author(s): 吉田松生
  • Organizer: 2019遺伝研研究会
  • Invited
• [Presentation] ひとつひとつの幹細胞のランダムなふるまいが安定した精子形成を支える (2019)
  • Author(s): 吉田松生
  • Organizer: 第17回幹細胞シンポジウム
  • Invited
• [Presentation] Unique differentiation processes support the sharp seasonal response of Japanese quail spermatogenesis. (2019)
  • Author(s): 吉田松生
  • Organizer: The annual meeting of the Japanese Society of Developmental Biologists
  • Int'l Joint Research
• [Presentation] Open niche regulation of spermatogenic stem cells in the mouse testis. (2018)
  • Author(s): S. Yoshida
  • Organizer: The 5th Conference of Frontiers in Reproductive Biology,
  • Int'l Joint Research / Invited
• [Presentation] Rapid and robust seasonal breeding in Japanese quail supported by unique process of spermatogenesis. (2018)
  • Author(s): S. Yoshida
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells
  • Int'l Joint Research
• [Presentation] Towards a Better Understanding of Spermatogenic Stem Cells. (2018)
  • Author(s): S. Yoshida
  • Organizer: The Society for the Study of Reproduction 51st Annual Conference
  • Int'l Joint Research / Invited
• [Presentation] Sperm stem cells: their context-dependent behavior. (2018)
  • Author(s): S. Yoshida
  • Organizer: ISSCR 2018
  • Int'l Joint Research / Invited
• [Presentation] 精子形成幹細胞のホメオスタシス維持システムとその経時変化 (2018)
  • Author(s): 吉田松生
  • Organizer: 千里ライフサイエンスセミナーM1＜老化メカニズムと疾患制御＞
  • Invited
• [Presentation] Flexible and stochastic behavior of spermatogenic stem cells. (2018)
  • Author(s): S. Yoshida
  • Organizer: The 20th European Testis Workshop
  • Int'l Joint Research / Invited
• [Presentation] For a Better Understanding of Spermatogenic Stem Cell Dynamics Over Time. (2018)
  • Author(s): S. Yoshida
  • Organizer: 11th NYRA Meeting, preceding the 20th European Testis Workshop
  • Int'l Joint Research / Invited
• [Presentation] 精子を作る細胞たち：個のランダム性が集団を支える (2018)
  • Author(s): 吉田松生
  • Organizer: 大隅基礎科学創成財団「第二回創発セミナー」
  • Invited
• [Presentation] 生殖細胞の凍結・移植による家禽遺伝資源の長期保存 (2018)
  • Author(s): 中村隼明
  • Organizer: 日本農芸化学会2018年度大会 シンポジウム「鳥類の農芸化学」
  • Invited
• [Presentation] Embryonic Development of the Blue-Breasted Quail (Coturnix chinensis). (2018)
  • Author(s): Nakamura Y, Nakane Y, Tsudzuki M
  • Organizer: Asian Australasian Animal Production Congress 2018
  • Int'l Joint Research
• [Presentation] 「家禽類および齧歯類における生殖細胞の移植を用いたキメラ作製技法」 (2018)
  • Author(s): 中村隼明
  • Organizer: 第111回日本繁殖生物学会大会 大会企画シンポジウム1「キメラ動物作製技術の応用」
  • Invited
• [Book] 遺伝学の百科事典 (2022)
  • Author(s): 日本遺伝学会
  • Total Pages: 690
  • Publisher: 丸善出版
  • ISBN: 9784621306604
• [Book] SDGsに向けた生物生産学入門 (2021)
  • Author(s): 三本木 至宏、上田 晃弘、杉野 利久、鈴木 卓弥、冨山 毅、船戸 耕一
  • Total Pages: 256
  • Publisher: 共立出版
  • ISBN: 9784320058316
• [Book] Regulatory mechanism of spermatogenic stem cells in mice: their dynamic and context-dependent behavior: PartⅠDivesity in Reproductive Stragegies and Germ Cell Differentiation Mechanisms, Animal Diverdity and Gnerality, “Reproductive and Developmental Strategies” (2018)
  • Author(s): S. Yoshida
  • Total Pages: 789
  • Publisher: Springer Japan KK
• [Remarks] 基礎生物学研究所 吉田研究室
  • URL: http://www.nibb.ac.jp/germcell/