Measurement of organic metabolism using low-energy photons
Project Area | Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process |
Project/Area Number |
19056003
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Science and Engineering
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Research Institution | Osaka University |
Principal Investigator |
AWAZU Kunio 大阪大学, 大学院・工学研究科, 教授 (30324817)
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Co-Investigator(Kenkyū-buntansha) |
HAZAMA Hisanao 大阪大学, 大学院・工学研究科, 助教 (70437375)
ISHII Katsunori 大阪大学, 大学院・工学研究科, 助教 (20512073)
鈴木 幸子 大阪大学, 大学院・工学研究科, 助教 (20403157)
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Project Period (FY) |
2007 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥35,000,000 (Direct Cost: ¥35,000,000)
Fiscal Year 2011: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2010: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2009: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2008: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2007: ¥8,400,000 (Direct Cost: ¥8,400,000)
|
Keywords | 質量分析 / タンパク質 / イオン化メカニズム / 中赤外レーザー / MALDI / 試料溶媒 / 水 / 難溶性タンパク質 / CARS / 低エネルギーフォトン / 蛋白質分析 / イオン化 / 赤外分光法 / 再生医療 |
Research Abstract |
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is widely used in the analysis of biomolecules such as proteins. However, the mechanism of MALDI has not been fully understood. If the MALDI process is elucidated and controlled, further progress in the biomedical fields is expected. In this study, we have used a mid-infrared laser instead of a conventional ultraviolet laser for MALDI and have succeeded in ionizing an insoluble analyte which has been hard to ionize. In addition, we have found that the wavelength dependence of the ionization efficiency of peptides is correlated with the chemical structure of the matrix and that the water solvent has influence on the ionization with MALDI.
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Report
(7 results)
Research Products
(75 results)
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[Presentation] Observation of tissue sections using a stigmatic mass microscope with a multi-turn time-of-flight mass spectrometer2011
Author(s)
Hisanao Hazama, Hidetoshi Yoshimura, Jun Aoki, Hirofumi Nagao, Yasuhide Naito, Michisato Toyoda, Katsuyoshi Masuda, Kenichi Fujii, Toshio Tashima, Kunio Awazu
Organizer
59th ASMS Conference on Mass Spectrometry and Allied Topics
Place of Presentation
Denver, CO, USA
Year and Date
2011-06-07
Related Report
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[Presentation] Observation of tissue sections using a stigmatic mass microscope with a multi-turn time-of-flight mass spectrometer2011
Author(s)
Hisanao Hazama, Hidetoshi Yoshimura, Jun Aoki, Hirofumi Nagao, Yasuhide Naito, Michisato Toyoda, Katsuyoshi Masuda, Kenichi Fujii, Toshio Tashima, Kunio Awazu
Organizer
59th ASMS Conference on Mass Spectrometry and Allied Topics
Place of Presentation
Colorado Convention Center, Denver, CO, USA
Year and Date
2011-06-07
Related Report
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[Presentation] Development of a stigmatic imaging mass spectrometer using laser desorption/ionization2011
Author(s)
Kunio Awazu, Hisanao Hazama, Hirofumi Nagao, Hidetoshi Yoshimura, Jun Aoki, Kenichi Fuji, Katsuyoshi Masuda, Toshio Tashima, Michisato Toyoda, Yasuhide Naito
Organizer
SPIE Photonics West, BiOS 2011
Place of Presentation
San Francisco, CA, USA
Related Report
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[Presentation] Development of a stigmatic mass microscope with laser ionization and a multi-turn time-of-flight mass spectrometer2010
Author(s)
Hisanao Hazama, Hidetoshi Yoshimura, Jun Aoki, Hirofumi Nagao, Yasuhide Naito, Michisato Toyoda, Katsuyoshi Masuda, Kenichi Fujii, Toshio Tashima, Kunio Awazu
Organizer
Saratov Fall Meeting
Place of Presentation
Saratov, Russia(Invited)
Related Report
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[Presentation] Observation of tissue sections stained with dyes using a stigmatic imaging mass spectrometer2010
Author(s)
Hisanao Hazama, Hidetoshi Yoshimura, Jun Aoki, Hirofumi Nagao, Yasuhide Naito, Michisato Toyoda, Katsuyoshi Masuda, Kenichi Fujii, Toshio Tashima, Kunio Awazu
Organizer
58th ASMS Conference on Mass Spectrometry and Allied Topics
Place of Presentation
Salt Lake City, UT, USA
Related Report
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[Presentation] Mid Infrared Laser Therapy2010
Author(s)
Kunio Awazu, Katsunori Ishii, Hisanao Hazama
Organizer
The 7th Asia-Pacific Laser Symposium (APLS 2010)
Place of Presentation
Seogwipo KAL Hotel, Jeju Island, Korea(招待講演)
Related Report
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[Presentation] Measurement of organic metabolism using low-energy photons2009
Author(s)
Kunio Awazu, Satoshi Furukawa, Katsunori Ishii, Hisanao Hazama
Organizer
3rd International Symposium on Molecular Science for Supra Functional Systems-Development of Advanced Methods for Exploring Elementary Processes
Place of Presentation
Digital Multi-purpose Hall, Tokyo Institute of Technology
Year and Date
2009-06-04
Related Report
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