Study of membrane binding protein complex using total internal reflection fluorescence correlation spectroscopy
| Project Area | Protein community: organization and maintenance of protein functions |
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| Project/Area Number |
19058001
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hokkaido University |
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Principal Investigator |
KINJO Masataka 北海道大学, 大学院・先端生命科学研究院, 教授 (70177971)
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| Co-Investigator(Kenkyū-buntansha) |
MIKUNI Shintaro 北海道大学, 大学院・医学研究科, 特任助教 (40435954)
KITAMURA Akira 北海道大学, 大学院・先端生命科学研究院, 助教 (10580152)
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| Project Period (FY) |
2007 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥71,000,000 (Direct Cost: ¥71,000,000)
Fiscal Year 2011: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2010: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2009: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2008: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2007: ¥14,200,000 (Direct Cost: ¥14,200,000)
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| Keywords | 生物物理 / 生体分子 / 蛍光測定 / 分子集合体 / 一分子検出 / 凝集タンパク質 / 酵母プリオン / 1分子検出 / 蛍光相互相関分析 / タンパク質相互作用 / シャペロン / タンパク質凝集体 / 分布関数 |
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| Research Abstract |
The functional molecules move around in the cell and interact with other molecules, and then support higher function of the cell. Moreover, even aggregate prone protein acts as aggregate and disaggregate under specific condition, so that each element of these aggregate protein is exchanged with free its molecules in solution. FCS (fluorescence correlation spectroscopy) detects the fluorescence fluctuations, the diffusion coefficient, molecular concentration and molecular interactions of molecules. We developed a multipoint FCS system which was based on an objective-type total internal reflection-FCS (TIR-FCS) in order to analysis molecular interaction in living cell and system of protein community.
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Report
(7 results)
Research Products
(157 results)
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[Journal Article] STPR, a 23-amino acid tandem repeat domain, found in the human function-unknown protein ZNF8212010
Author(s)
Nonaka Y, Muto H, Aizawa T, Okabe E, Myoba S, Yokoyama T, Saito S, Tatami F, Kumaki Y, Kamiya M, Kikukawa T, Mizuguchi M, Takiya S, Kinjo M, Demura M, Kawano K.
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Journal Title
Biochemistry
Volume: 49(38)
Issue: 38
Pages: 8367-8375
DOI
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Peer Reviewed
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[Journal Article] The A-and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription2008
Author(s)
Shimi T, Pfleghaar K, Kojima S, Pack CG, Solovei I, Goldman AE, Adam SA, Shumaker DK, Kinjo M, Cremer T, Goldman RD.
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Journal Title
Genes Dev.
Volume: 22(24)
Issue: 24
Pages: 3409-3421
DOI
Related Report
Peer Reviewed
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[Journal Article] Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking2008
Author(s)
Noda Y, Horikawa S, Kanda E, Yamashita M, Meng H, Eto K, Li Y, Kuwahara M, Hirai K, Pack C, Kinjo M, Okabe S, Sasaki S.
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Journal Title
J Cell Biol.
Volume: 182(3)
Issue: 3
Pages: 587-601
DOI
Related Report
Peer Reviewed
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[Presentation] FCSとFCCS2009
Author(s)
金城政孝
Organizer
第5回ライブセルイメージング講習会
Place of Presentation
理化学研究所(和光市)
Year and Date
2009-10-06
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