Quality control of E. coli membrane proteins
Project Area | Protein community: organization and maintenance of protein functions |
Project/Area Number |
19058007
|
Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
|
Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
|
Research Institution | Kyoto University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
MORI Hiroyuki 京都大学, ウイルス研究所, 准教授 (10243271)
|
Project Period (FY) |
2007 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥72,000,000 (Direct Cost: ¥72,000,000)
Fiscal Year 2011: ¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2010: ¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2009: ¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2008: ¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2007: ¥14,400,000 (Direct Cost: ¥14,400,000)
|
Keywords | 膜タンパク質 / 品質管理 / 膜プロテアーゼ / ストレス応答 / タンパク質膜透過 / 膜内切断プロテアーゼ / ロンボイド / 基質特異性 / 大腸菌 / RIP / 表層ストレス応答 / SecDF / プロトン駆動力 / purotea-ze |
Research Abstract |
We studied the structures and functions of cellular factors participating in quality control of E. coli membrane proteins. We revealed the cellular functions and substrate recognition mechanisms of intramembrane cleaving proteases, RseP (S2P family) and GlpG (Rhomboid family). We also elucidated the structures of SecYE (translocon) and SecDF, membrane complexes involved in protein translocation, and proposed the mechanism of their actions. In addition, we identified proteins whose functions would be related to ‘membrane stress responses'.
|
Report
(7 results)
Research Products
(58 results)
-
-
[Journal Article] Nascentome analysis uncovers futile protein synthesis in Escherichia coli2011
Author(s)
Ito, K., Chadani, Y., Nakamori, K., Chiba, S., Akiyama, Y., and Abo, T.
-
Journal Title
Related Report
Peer Reviewed
-
[Journal Article] Post liberation cleavage of signal peptides is catalyzed by the S2P protease in bacteria2011
Author(s)
Saito, A., Hizukuri, Y., Matsuo, E.-i., Chiba, S., Mori, H., Nishimura, O., Ito, K., and Akiyama, Y.
-
Journal Title
Proc. Natl. Acad. Sci, USA
Volume: 108
Pages: 13740-13745
Related Report
Peer Reviewed
-
[Journal Article] Structure and function of SecDF, a protein export-enhancing membrane component2011
Author(s)
Tsukazaki, T., Mori, H. , Echizen, Y., Ishitani, R., Fukai, S., Tanaka, T, Perederina, A., Vassylyev, D. G., Kohno, T., Maturana, A. D., Ito, K. and Nureki, O.
-
Journal Title
Nature
Volume: 474
Pages: 235-238
Related Report
Peer Reviewed
-
[Journal Article] Recruitment of a species-specific arrest module to monitor different cellular processes2011
Author(s)
Chiba. S., Kanamori, T., Ueda, T., Akiyama, Y., Pogliano, K. and Ito, K.
-
Journal Title
Proc. Natl. Acad. Sci, USA
Volume: 108
Pages: 6073-6078
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
[Journal Article] Conformational transition of Sec machinery inferred from bacterial SecYE structures2008
Author(s)
Tsukazaki, T., Mori, H., Fukai, S., Ishitani, R., Mori, T., Dohmae, N., Perederina, A., Sugita, Y., Vassylyev, D. G., Ito, K. and Nureki, O.
-
Journal Title
Nature
Volume: 455
Pages: 988-991
NAID
Related Report
Peer Reviewed
-
[Journal Article] A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of E. coli.2008
Author(s)
Inaba, K., Suzuki, M., Maegawa, K., Akiyama, S., Ito, K., and Akiyama, Y.
-
Journal Title
J. Biol. Chem.
Volume: 283
Pages: 35042-35052
Related Report
Peer Reviewed
-
[Journal Article] Conformational transition of Sec machinery inferred from bacterial SecYE structures.2008
Author(s)
Tsukazaki, T., Mori, H., Fukai, S., Ishitani, R., Mori, T., Dohmae, N., Perederina, A., Sugita, Y., Vassylyev, D.G., Ito, K., Nureki O.
-
Journal Title
Nature 455
Pages: 988-991
NAID
Related Report
Peer Reviewed
-
[Journal Article] A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of E. coli.2008
Author(s)
Inaba, K., Suzuki, M., Maegawa, K., Akiyama, S., Ito, K., Akiyama, Y.
-
Journal Title
J. Biol. Chem. 283
Pages: 35042-35052
Related Report
Peer Reviewed
-
-
[Journal Article] A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of E. coli2008
Author(s)
Inaba, K., Suzuki, M., Maegawa, K., Akiyama, S., Ito, K., and Akiyama, Y.
-
Journal Title
J. Biol. Chem 283
Pages: 35042-35052
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-