| Budget Amount *help |
¥137,280,000 (Direct Cost: ¥105,600,000、Indirect Cost: ¥31,680,000)
Fiscal Year 2023: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2022: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2021: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2020: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2019: ¥38,090,000 (Direct Cost: ¥29,300,000、Indirect Cost: ¥8,790,000)
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| Outline of Final Research Achievements |
We’ve explored the links between reconstitution of nucleosome and Polycomb proteins during DNA replication by focusing on PRC1 and Enhancer of Polycomb (EpC). We revealed that in hematopoietic progenitor cells (HPCs), PCGF1-PRC1 localized in the vicinity of the replication fork to prevent aberrant accession of chromatin remodeling factors, thereby maintaing proper nucleosome densities immediately after the passage of the fork. Furthermore, by safeguarding nucleosome reconstitution, PCGF-PRC1 facilitates H3K27me3-mediated downregulation of myeloid-related genes to restrict myeloid properties in HPCs. It has been recognized that the balance between activators and repressors is critical to mediate normal differentiation and cell proliferation. Our findings highlight that this counteraction involving PCGF1-PRC1 occurs in the vicinity of the replication fork. Moreover, we also found that EpC contributed to the process of reconstitution of nucleosome.
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