|Budget Amount *help
¥102,600,000 (Direct Cost: ¥102,600,000)
Fiscal Year 2012: ¥19,700,000 (Direct Cost: ¥19,700,000)
Fiscal Year 2011: ¥19,700,000 (Direct Cost: ¥19,700,000)
Fiscal Year 2010: ¥23,700,000 (Direct Cost: ¥23,700,000)
Fiscal Year 2009: ¥23,700,000 (Direct Cost: ¥23,700,000)
Fiscal Year 2008: ¥15,800,000 (Direct Cost: ¥15,800,000)
Although it is well accepted that spermatogenesis and spermiogenesis are accompanied by sequential changes of chemical modifications of histone tails, their biological impacts and underlying mechanisms are poorly understood until now. In this study, we focus on the role of H3K9 methylation and H2A monoubiquitination mediated by Polycomb complexes during meiotic prophase and histone hyperacetylation in round spermatids. We found H3K9 methylation and its recognition played critical role to regulate centromere dynamics and chromosome pairing. Polycomb turned out to possess distinct labor for chromosome pairing by promoting telomere/nuclear envelope interaction. We finally demonstrated mammalian ortholog of yeast piccolo complexes were essential for global histone hyperacetylation in round spermatids and for histone/TP replacement.