|Budget Amount *help
¥56,550,000 (Direct Cost: ¥43,500,000、Indirect Cost: ¥13,050,000)
Fiscal Year 2012: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2011: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2010: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2009: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
We successfully developed a stem cell rolling column with a soft-interface bearing anti cell surface marker molecules antibody. A clued mesenchymal stem cell suspension was separated using these column and two different subpopulation of MSC was discovered in CD34 low and high franctions. We came up with a big problem about the non-specific cell entrapment on the antibody-immobilized column, and it reduces the efficiency of specific cell purification based on cell-rolling. To overcome this problem, newly modified surfaces were designed and evaluated for the cell-rolling surface. Hydrophilic polymer grafting, silane coupling reagents, and SAM on Au were selected and evaluated for the cell-rolling surfaces. As the results, zwitterionic telomer brush containing 2-methacryloyloxyethyl phosphorylcholine (MPC) efficiency suppressed the non-specific interaction between cells and antibody-immobilized surface. Finally, poly[MPC-co-n-butyl mechacrylate (nBMA)-co-N-vinylformamide (NVF)] copolymers were found to be very effective to suppress the interaction and achieved the very stable cell rolling and excellent cell separation property.