|Budget Amount *help
¥87,880,000 (Direct Cost: ¥67,600,000、Indirect Cost: ¥20,280,000)
Fiscal Year 2012: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2011: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2010: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2009: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2008: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
In this study, an improved method for saturation mutagenesis allowing the substitution with natural and nonnatural amino acids to investigate the effect of the mutagenesis on proteins was developed. In addition, we developed a method for structural and functional protein analysis by introducing fluorescent nonnatural amino acids. Practical application of nonnatural amino acid-containing proteins as analytical tools and large-scale production of nonnatural proteins were also investigated.