mRNA regulation in germ cell formation

• Project Area: Diversity and asymmetry achieved by RNA program
• Project/Area Number: 20112003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kobe University
• Principal Investigator: INOUE Kunio 神戸大学, 大学院・理学研究科, 教授 (40252415)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥108,810,000 (Direct Cost: ¥83,700,000、Indirect Cost: ¥25,110,000); Fiscal Year 2012: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2011: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2010: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2009: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2008: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
• Keywords: 生殖細胞 / 生殖顆粒 / mRNA 制御 / miRNA / RNA 結合蛋白質 / TALEN 法 / 発現制御 / 発生・分化 / mRNA / 翻訳制御 / TALEN法 / 生殖質 / mRNA制御 / RNA結合蛋白質

Research Abstract

In zebrafish, primordial germ cells (PGCs) are determined by a specialized maternal cytoplasm, germ plasm, which forms at the distal ends of the cleavage furrows in 4-cell embryos. During embryogenesis, miR-430 represses some germ plasm mRNAs incorporated into somatic cells. Here we showed that a germline-specific RNA-binding protein DAZL counteracts the miRNA-mediated repression of tdrd7 and dazl mRNAs. Also we showed that, in zebrafish embryos, miRNA inhibits translation of the target mRNA in a deadenylationand PABP-independent manner at early time points.

Research Products

• [Journal Article] Characterization of the compact bicistronic microRNA precursor, miR-1/miR-133, expressed specifically in Ciona muscle tissues (2013)
  • Author(s): Kusakabe, R., Tani, S., Nishitsuji, K., Shindo, M., Okamura, K., Miyamoto, Y., Nakai, K., Suzuki, Y., Kusakabe, T. G., and Inoue, K
  • Journal Title: Gene Exp. Patterns Volume: 13 Pages: 43-50
  • Peer Reviewed
• [Journal Article] A specific set of exon junction complex subunits is required for the nuclear retention of unspliced RNAs in Caenorhabditis elegans (2013)
  • Author(s): Shiimori, M., Inoue, K., and Sakamoto, H
  • Journal Title: Mol. Cell. Biol Volume: 33 Pages: 444-456
  • Peer Reviewed
• [Journal Article] A specific set of EJC subunits is required for the nuclear retention of unspliced RNAs in C. elegans (2013)
  • Author(s): Masami Shiimori, KunioInoue, Hiroshi Sakamoto
  • Journal Title: Molecular and Cellular Biology Volume: Jan ; 33(2) Issue: 2 Pages: 444-56
  • DOI: 10.1128/mcb.01298-12
  • Peer Reviewed
• [Journal Article] Characterization of the compact bicistronic microRNA precursor, miR-1/miR-133, expressed specifically in Ciona muscle tissues. (2013)
  • Author(s): Rie Kusakabe, Saori Tani, Koki Nishitsuji, Miyuki Shindo, Kohji Okamura, Yuki Miyamoto, Kenta Nakai, Yutaka Suzuki, Takehiro G Kusakabe, Kunio Inoue
  • Journal Title: Gene Expression Patterns Volume: 13 Issue: 1-2 Pages: 43-50
  • DOI: 10.1016/j.gep.2012.11.001
  • Peer Reviewed
• [Journal Article] Identification of MIWI-associated poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody (2012)
  • Author(s): Nishibu, T., Hayashida, Y., Tani, S., Kurono, S., Ukekawa, R., Kojima-Kita, K., Kurokawa, T., Kuramochi-Miyagawa, S., Nakano, T., Inoue, K., and Honda, S
  • Journal Title: BioScience Trends Volume: 6 Pages: 248-261
  • NAID: 130005054634
  • Peer Reviewed
• [Journal Article] Translational inhibition by deadenylation-independent mechanisms is central to microRNA-mediated silencing in zebrafish (2012)
  • Author(s): Mishima, Y., Fukao, A., Kishimoto, T., Sakamoto, H., Fujiwara, T., and Inoue, K
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Pages: 1104-1109
  • Peer Reviewed
• [Journal Article] Functional and direct interaction between the RNA binding protein HuD and active Akt1 (2012)
  • Author(s): Fujiwara, T., Fukao, A., Sasano, Y., Matsuzaki, H., Kikkawa, U., Imataka, A., Inoue, K., Endo, S., Sonenberg, N. Thoma, C., and Sakamoto, H
  • Journal Title: Nucleic Acids Res Volume: 40 Pages: 1944-1953
  • Peer Reviewed
• [Journal Article] Identification of MIWI-associated poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody. (2012)
  • Author(s): Nishibu, T. et al.
  • Journal Title: BioScience Trends Volume: 6 Pages: 248-261
  • Peer Reviewed
• [Journal Article] Translational inhibition by deadenylation-independent mechanismsis central to microRNA-mediated silencing in zebrafish (2012)
  • Author(s): Yuichiro Mishima
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 109(4) Issue: 4 Pages: 1104-1109
  • DOI: 10.1073/pnas.1113350109
  • Peer Reviewed
• [Journal Article] Functional and direct interaction between the RNA binding protein HuD and active Akt1 (2012)
  • Author(s): Toshinobu Fujiwara
  • Journal Title: Nucleic Acids Research Volume: 40(5) Issue: 5 Pages: 1944-1953
  • DOI: 10.1093/nar/gkr979
  • Peer Reviewed
• [Journal Article] Microtubule association of a neuronal RNA-binding protein HuD through its binding to the light chain of MAP1B (2011)
  • Author(s): Fujiwara, Y., Kasashima, K., Saito, K., Fukuda, M., Fukao, A., Sasano, Y., Inoue, K., Fujiwara, T., and Sakamoto, H
  • Journal Title: Biochimie Volume: 93 Pages: 817-822
  • Peer Reviewed
• [Journal Article] Microtubule association of a neuronal RNA-binding protein HuD through its binding to the light chain of MAP1B. (2011)
  • Author(s): Fujiwara, Yukiko.
  • Journal Title: Biochimie Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Genomic organization and embryonic expression of miR-430 in medaka (Oryzias latipes): insights into the post-transcriptional gene regulation in early development (2010)
  • Author(s): Tani, S., Kusakabe, R., Naruse, K., Sakamoto, H., and Inoue, K
  • Journal Title: Gene Volume: 449 Pages: 41-49
  • Peer Reviewed
• [Journal Article] Genomic organization and embryonic expression of miR-430 in medaka (Oryzias latipes) : insights into the post-transcriptional gene regulation in early development. (2010)
  • Author(s): Tani, S., Kusakabe, R., Naruse, K., Sakamoto, H., Inoue, K.
  • Journal Title: Gene 449 Pages: 41-49
  • Peer Reviewed
• [Journal Article] Genomic organization and embryonic expression of miR-430 in medaka (Oryzias Latipes) : insights into the post-transcriptional gene regulation in early devlopment. (2010)
  • Author(s): Tani, S., et al.
  • Journal Title: Gene 449 Pages: 41-49
  • Peer Reviewed
• [Journal Article] DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish (2009)
  • Author(s): Takeda, Y., Mishima, Y., Fujiwara, T., Sakamoto, H., and Inoue, K
  • Journal Title: PLoS ONE Volume: 4
  • Peer Reviewed
• [Journal Article] The ELAV protein HuD stimulates cap-dependent translation in a poly(A)- and eIF4A-dependent manner (2009)
  • Author(s): Fukao, A., Sasano, Y., Imataka, H., Inoue, K., Sakamoto, H., Sonenberg, N., Thoma, C. and Fujiwara, T
  • Journal Title: Mol. Cell Volume: 36 Pages: 1007-1017
  • Peer Reviewed
• [Journal Article] A useful approach to total analysis of RISC-associated RNA (2009)
  • Author(s): Hayashida, Y., Nishibu, T., Inoue, K., and Kurokawa, T
  • Journal Title: BMC Research Notes Volume: 2 Pages: 169-169
  • Peer Reviewed
• [Journal Article] Role and mechanism of U1-independent pre-mRNA splicing in the regulation of alternative splicing (2009)
  • Author(s): Fukumura, K. and Inoue, K
  • Journal Title: RNA Biol Volume: 6 Pages: 395-398
  • Peer Reviewed
• [Journal Article] A novel application of metabolomics in vertebrate development (2009)
  • Author(s): Hayashi, S., Akiyama, S., Tamaru, Y., Takeda, Y., Fujiwara, T., Inoue, K., Kobayashi, A., Maegawa, S., and Fukusaki, E
  • Journal Title: Biochem Biophys Res Commun Volume: 386 Pages: 268-272
  • Peer Reviewed
• [Journal Article] Localization of c-mos mRNA around the animal pole in the zebrafish oocyte with Zor-1/Zorba (2009)
  • Author(s): Suzuki, H., Tsukahara, T., and Inoue, K
  • Journal Title: BioScience Trends Volume: 3 Pages: 96-104
  • Peer Reviewed
• [Journal Article] U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing (2009)
  • Author(s): Fukumura, K., Taniguchi, I., Sakamoto, H., Ohno, M., and Inoue, K
  • Journal Title: Nucleic Acids Research Volume: 37 Pages: 1907-1914
  • Peer Reviewed
• [Journal Article] DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish. (2009)
  • Author(s): Takeda, Y., Mishima, Y., Fujiwara, T., Sakamoto, H., Inoue, K.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] The ELAV protein HuD stimulates cap-dependent translation in a poly(A)- and eIF4A-dependent manner. (2009)
  • Author(s): Fukao, A., Sasano, Y., Imataka, H., Inoue, K., Sakamoto, H., Sonenberg, N., Thoma, C., Fujiwara, T.
  • Journal Title: Mol.Cell 36 Pages: 1007-1017
  • Peer Reviewed
• [Journal Article] Localization of c-mos mRNA around the animal pole in the zebrafish oocyte with Zor-1/Zorba. (2009)
  • Author(s): Suzuki, H., Tsukahara, T., Inoue, K.
  • Journal Title: BioScience Trends 3 Pages: 96-104
  • Peer Reviewed
• [Journal Article] U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing. (2009)
  • Author(s): Fukumura, K., Taniguchi, I., Sakamoto, H., Ohno, M., Inoue, K.
  • Journal Title: Nucl.Acids Res. 37 Pages: 1907-1914
  • Peer Reviewed
• [Journal Article] DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly (A) tail length in zebrafish. (2009)
  • Author(s): Takeda, Y., et al.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] 生殖細胞と体細胞の分化とmiRNAの関与 (2008)
  • Author(s): 井上邦夫
  • Journal Title: 生体の科学 59 Pages: 356-357
• [Journal Article] R. Developmental expression and evolution of muscle-specific microRNAs conserved in vertebrates
  • Author(s): Tani, S., Kuraku, S., Sakamoto, H., Inoue, K., and Kusakabe, R
  • Journal Title: Evolution & Development Volume: (in press)
  • Peer Reviewed
• [Presentation] ゼブラフィッシュ胚発生を制御するmicroRNAシステムの分子基盤 (2012)
  • Author(s): 三嶋雄一郎
  • Organizer: 第３５回分子生物学会年会 ワークショップ「RNA制御の細胞生物学」
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] ゼブラフィッシュ生殖顆粒構成因子Granulitoタンパク質の機能解析 (2012)
  • Author(s): 稲川陽介
  • Organizer: 第３５回分子生物学会年会 ワークショップ「RNA制御の細胞生物学」
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] RNA regulation in zebrafish germ cell formation. (2012)
  • Author(s): Inoue, K.
  • Organizer: 2012 Taiwan-Japan Joint Symposium on cell signaling and gene regulation
  • Place of Presentation: 台南
  • Year and Date: 2012-11-11
  • Invited
• [Presentation] RNA regulation in zebrafish germ cell formation (2012)
  • Author(s): Inoue, K
  • Organizer: 2012 Taiwan-Japan Joint Symposium on cell signaling and gene regulation
  • Place of Presentation: 台南・台湾
• [Presentation] Molecular mechanisms and regulations of microRNA-mediated gene silencing inzebrafish (2011)
  • Author(s): Mishima, Y., and Inoue, K
  • Organizer: 第34回日本分子生物学会・ワークショップ
  • Place of Presentation: 横浜
• [Presentation] Molecular mechanisms and regulations of microRNA-mediated gene sile ncing in zebrafish (2011)
  • Author(s): Mishima, Yuichiro
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)(招待講演)
• [Presentation] Control of germline/somatic cell distinction by miRNA function in zebrafish. (2010)
  • Author(s): Inoue, Kunio
  • Organizer: A satellite symposium on Germ Cells in SDB-JSDB Joint Meeting 2010
  • Place of Presentation: Albuquerque Convention Center (New Mexico, USA)(招待講演)
  • Year and Date: 2010-08-05
• [Presentation] Control of germline/somatic cell distinction by miRNA function in zebrafish (2010)
  • Author(s): Inoue, K
  • Organizer: A satellite symposium on Germ Cells in SDB-JSDB Joint Meeting 2010
  • Place of Presentation: New Mexico.America
• [Presentation] Control of germline/somatic cell distinction by miRNA function in zebrafish. (2010)
  • Author(s): Inoue, K.
  • Organizer: A satellite symposium on Germ Cells in SDB-JSDB Joint Meeting 2010.
  • Place of Presentation: Albuquerque Convention Center (New Mexico, USA) 招待講演
• [Presentation] U1-independent splicing and the splicing regulation (2009)
  • Author(s): Inoue, K
  • Organizer: 第32回日本分子生物学会 ワークショップ「多様性を生むRNAプログラム」
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Control of germline/somatic cell distinctions by miRNA function (2009)
  • Author(s): Inoue, K
  • Organizer: The NIBB International Practical course "Developmental Genetics of zebrafish and medaka"
  • Place of Presentation: 基生研
  • Year and Date: 2009-01-29
• [Presentation] U1-independent pre-mRNA splicing contributes to the regulationof alternative splicing (2008)
  • Author(s): Inoue, K
  • Organizer: 国際シンポジウム "The expanding world of post-transcriptional regulation of gene expression"
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-12-13
• [Presentation] ゼブラフィッシュmiRNA制御システムによる生殖細胞形成機構 (2008)
  • Author(s): 井上邦夫
  • Organizer: 第41回日本発生生物学会・サテライトワークショップ
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-27
• [Remarks]
• [Remarks]
  • URL: http://www.org.kobe-u.ac.jp/rna/
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/fsci-rna/
• [Remarks] ホームページ
  • URL: http://www.research.kobe-u.ac.jp/fsci-rna/
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/fsci-rna/
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/fsci-rna/
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/fsci-rna/